Structured Review

Promega sars cov fragments
Measurement of <t>SARS-CoV</t> replication in the presence of SSYA10-001. (A) HEK 293T cells were mock transfected or transfected with the SARS-CoV replicon or a nonreplicative construct by use of Lipofectamine 2000 (Invitrogen) in the presence of various concentrations
Sars Cov Fragments, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sars cov fragments/product/Promega
Average 85 stars, based on 220 article reviews
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sars cov fragments - by Bioz Stars, 2021-01
85/100 stars

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1) Product Images from "Severe Acute Respiratory Syndrome Coronavirus Replication Inhibitor That Interferes with the Nucleic Acid Unwinding of the Viral Helicase"

Article Title: Severe Acute Respiratory Syndrome Coronavirus Replication Inhibitor That Interferes with the Nucleic Acid Unwinding of the Viral Helicase

Journal: Antimicrobial Agents and Chemotherapy

doi: 10.1128/AAC.00957-12

Measurement of SARS-CoV replication in the presence of SSYA10-001. (A) HEK 293T cells were mock transfected or transfected with the SARS-CoV replicon or a nonreplicative construct by use of Lipofectamine 2000 (Invitrogen) in the presence of various concentrations
Figure Legend Snippet: Measurement of SARS-CoV replication in the presence of SSYA10-001. (A) HEK 293T cells were mock transfected or transfected with the SARS-CoV replicon or a nonreplicative construct by use of Lipofectamine 2000 (Invitrogen) in the presence of various concentrations

Techniques Used: Transfection, Construct

Effect of SSYA10-001 on activity of other helicases and polymerases. (A) Plot of the fractions of unwound RNA for the HCV NS3h helicase (♢), dengue virus NS3 helicase (○), and SARS-CoV nsp13 helicase (■). Experiments were performed
Figure Legend Snippet: Effect of SSYA10-001 on activity of other helicases and polymerases. (A) Plot of the fractions of unwound RNA for the HCV NS3h helicase (♢), dengue virus NS3 helicase (○), and SARS-CoV nsp13 helicase (■). Experiments were performed

Techniques Used: Activity Assay

Related Articles

Clone Assay:

Article Title: Molecular Cloning and Mutagenesis of a DNA Locus Involved in Lipooligosaccharide Biosynthesis in Haemophilus somnus
Article Snippet: .. This fragment was cloned into the commercial vector pGEM-3Z to create pCAAT (Fig. ). ..

Functional Assay:

Article Title: Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and γδT Cells
Article Snippet: .. By sequencing analysis, we chose a cDNA clone for each of the TCRs that had a functional open reading frame, and subcloned them into Eco RI and Bam HI sites of pGEM-3Z vector (Promega). .. Human NKT-TCR used in this study is as follows, α-chain: TRAV10*01/TRAJ18*01/TRAC*01 (GenBank Accession # DQ341448); β-chain: TRBV25-1*01/TRBD1*01/TRBJ1-4*01/TRBC1*01, with CDR3β sequence being CASEQRGGVDEKL (V region; GenBank #DQ34146), J and C regions; Genbank #BC073930).

Polymerase Chain Reaction:

Article Title: Glial Cell Aquaporin-4 Overexpression in Transgenic Mice Accelerates Cytotoxic Brain Swelling *
Article Snippet: .. Transgenic Mice —A 1.54-kb fragment of mouse AQP4 cDNA (121–1661, GenBank™ accession number ), containing the full coding region (including the ATG translational start site and the TAG stop codon) and the 3′-untranslated and poly-A sequences, was PCR-amplified from mouse brain cDNA and subcloned into pGEM3Z (Promega). .. A cytomegalovirus enhancer sequence (∼0.3 kb, subcloned from pcDNA3, Invitrogen) and a PCR-amplified ∼2.2-kb GFAP promoter sequence (4–2177, GenBank accession number ) were inserted upstream of the AQP4 cDNA sequence (see ).

Isolation:

Article Title: Unique Characteristics of a Picornavirus Internal Ribosome Entry Site from the Porcine Teschovirus-1 Talfan
Article Snippet: .. The product was digested with Bam HI and Sph I, and the fragment, including the PTV 5′UTR and myc-tagged coding sequence, was isolated and ligated into similarly digested pGEM3Z (Promega) to create pGEM-IB (Fig. ). .. This plasmid directs the expression of the PTV RNA (nt 1 to 1544) under control of the T7 promoter.

Mouse Assay:

Article Title: Glial Cell Aquaporin-4 Overexpression in Transgenic Mice Accelerates Cytotoxic Brain Swelling *
Article Snippet: .. Transgenic Mice —A 1.54-kb fragment of mouse AQP4 cDNA (121–1661, GenBank™ accession number ), containing the full coding region (including the ATG translational start site and the TAG stop codon) and the 3′-untranslated and poly-A sequences, was PCR-amplified from mouse brain cDNA and subcloned into pGEM3Z (Promega). .. A cytomegalovirus enhancer sequence (∼0.3 kb, subcloned from pcDNA3, Invitrogen) and a PCR-amplified ∼2.2-kb GFAP promoter sequence (4–2177, GenBank accession number ) were inserted upstream of the AQP4 cDNA sequence (see ).

Transgenic Assay:

Article Title: Glial Cell Aquaporin-4 Overexpression in Transgenic Mice Accelerates Cytotoxic Brain Swelling *
Article Snippet: .. Transgenic Mice —A 1.54-kb fragment of mouse AQP4 cDNA (121–1661, GenBank™ accession number ), containing the full coding region (including the ATG translational start site and the TAG stop codon) and the 3′-untranslated and poly-A sequences, was PCR-amplified from mouse brain cDNA and subcloned into pGEM3Z (Promega). .. A cytomegalovirus enhancer sequence (∼0.3 kb, subcloned from pcDNA3, Invitrogen) and a PCR-amplified ∼2.2-kb GFAP promoter sequence (4–2177, GenBank accession number ) were inserted upstream of the AQP4 cDNA sequence (see ).

Sequencing:

Article Title: Unique Characteristics of a Picornavirus Internal Ribosome Entry Site from the Porcine Teschovirus-1 Talfan
Article Snippet: .. The product was digested with Bam HI and Sph I, and the fragment, including the PTV 5′UTR and myc-tagged coding sequence, was isolated and ligated into similarly digested pGEM3Z (Promega) to create pGEM-IB (Fig. ). .. This plasmid directs the expression of the PTV RNA (nt 1 to 1544) under control of the T7 promoter.

Article Title: Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and γδT Cells
Article Snippet: .. By sequencing analysis, we chose a cDNA clone for each of the TCRs that had a functional open reading frame, and subcloned them into Eco RI and Bam HI sites of pGEM-3Z vector (Promega). .. Human NKT-TCR used in this study is as follows, α-chain: TRAV10*01/TRAJ18*01/TRAC*01 (GenBank Accession # DQ341448); β-chain: TRBV25-1*01/TRBD1*01/TRBJ1-4*01/TRBC1*01, with CDR3β sequence being CASEQRGGVDEKL (V region; GenBank #DQ34146), J and C regions; Genbank #BC073930).

Purification:

Article Title: Unique Characteristics of a Picornavirus Internal Ribosome Entry Site from the Porcine Teschovirus-1 Talfan
Article Snippet: .. The fragments, of about 1.4, 1.3, and 1.05 kbp, respectively, were purified, digested with Bam HI and Sph I, and then ligated into similarly digested pGEM3Z to generate pGEM-T1B, pGEM-T2B, and pGEM-T3B, respectively (Fig. ). ..

Plasmid Preparation:

Article Title: Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and γδT Cells
Article Snippet: .. By sequencing analysis, we chose a cDNA clone for each of the TCRs that had a functional open reading frame, and subcloned them into Eco RI and Bam HI sites of pGEM-3Z vector (Promega). .. Human NKT-TCR used in this study is as follows, α-chain: TRAV10*01/TRAJ18*01/TRAC*01 (GenBank Accession # DQ341448); β-chain: TRBV25-1*01/TRBD1*01/TRBJ1-4*01/TRBC1*01, with CDR3β sequence being CASEQRGGVDEKL (V region; GenBank #DQ34146), J and C regions; Genbank #BC073930).

Article Title: Molecular Cloning and Mutagenesis of a DNA Locus Involved in Lipooligosaccharide Biosynthesis in Haemophilus somnus
Article Snippet: .. Probes were also made to a 630-bp Sal I- Bsg I fragment within lob-2A and to the vector pGEM-3Z (Promega Corp.) by using the entire 2.7-kb plasmid as a template. .. DNA hybridizations were performed at 68°C in 5× SSC containing 0.1% N -lauroylsarcosine, 0.025% sodium dodecyl sulfate (SDS), and 1% Genius blocking reagent.

Article Title: Molecular Cloning and Mutagenesis of a DNA Locus Involved in Lipooligosaccharide Biosynthesis in Haemophilus somnus
Article Snippet: .. This fragment was cloned into the commercial vector pGEM-3Z to create pCAAT (Fig. ). ..

Article Title: Molecular Cloning and Mutagenesis of a DNA Locus Involved in Lipooligosaccharide Biosynthesis in Haemophilus somnus
Article Snippet: .. In addition, probes to the Sal I- Bsg I 630-bp deletion from lob-2A and to vector pGEM-3Z failed to hybridize to 738-lob2A1::Km (data not shown). ..

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    Promega t7 driven pgem 3z transcription vector
    T7 Driven Pgem 3z Transcription Vector, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 driven pgem 3z transcription vector/product/Promega
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t7 driven pgem 3z transcription vector - by Bioz Stars, 2021-01
    97/100 stars
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