Structured Review

Enzymatics t4 rna ligase 2
T4 Rna Ligase 2, supplied by Enzymatics, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 rna ligase 2/product/Enzymatics
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
t4 rna ligase 2 - by Bioz Stars, 2020-09
92/100 stars

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Electrophoresis:

Article Title: Dynamic protein–RNA interactions in mediating splicing catalysis
Article Snippet: .. The mixtures were incubated at 65°C for 20 min, shifted to 37°C for 15 min, and then 25°C for 20 min. After addition of 11.5 μl dH2 O, 1.25 μl of 16 mM ATP, 7.5 μl of 50% PEG-8000, 1 μl of RNasin, and 5 μl of T4 RNA ligase 2, the mixtures were incubated at 37°C for 2 h. The ligated products were purified by electrophoresis on 5% denaturing polyacrylamide gels. .. Immunoprecipitation and immunodepletion Immunoprecipitation of the spliceosome was performed as described ( ).

Incubation:

Article Title: Dynamic protein–RNA interactions in mediating splicing catalysis
Article Snippet: .. The mixtures were incubated at 65°C for 20 min, shifted to 37°C for 15 min, and then 25°C for 20 min. After addition of 11.5 μl dH2 O, 1.25 μl of 16 mM ATP, 7.5 μl of 50% PEG-8000, 1 μl of RNasin, and 5 μl of T4 RNA ligase 2, the mixtures were incubated at 37°C for 2 h. The ligated products were purified by electrophoresis on 5% denaturing polyacrylamide gels. .. Immunoprecipitation and immunodepletion Immunoprecipitation of the spliceosome was performed as described ( ).

Ligation:

Article Title: Barcoding bias in high-throughput multiplex sequencing of miRNA
Article Snippet: .. Ligation of the 3′ adapter was conducted by incubating 1 μg of total RNA from the desired samples with 10 pmol of 3′ adenylated oligonucleotide, 10% DMSO, 20 U of RNaseInhibitor (Enzymatics Y924L), and 300 U of T4 RNA ligase 2 truncated (Enzymatics), for 1 h at 22°C. .. Following incubation, 10 pmol of 5′ adapter was added alongside 8 μM ATP (Enzymatics N207-10-L) and 20 U of T4 RNA Ligase 1 (Enzymatics L605L) and was incubated for 1 h at 20°C.

Purification:

Article Title: Dynamic protein–RNA interactions in mediating splicing catalysis
Article Snippet: .. The mixtures were incubated at 65°C for 20 min, shifted to 37°C for 15 min, and then 25°C for 20 min. After addition of 11.5 μl dH2 O, 1.25 μl of 16 mM ATP, 7.5 μl of 50% PEG-8000, 1 μl of RNasin, and 5 μl of T4 RNA ligase 2, the mixtures were incubated at 37°C for 2 h. The ligated products were purified by electrophoresis on 5% denaturing polyacrylamide gels. .. Immunoprecipitation and immunodepletion Immunoprecipitation of the spliceosome was performed as described ( ).

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    Enzymatics t4 rna ligase 2
    T4 Rna Ligase 2, supplied by Enzymatics, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase 2/product/Enzymatics
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase 2 - by Bioz Stars, 2020-09
    92/100 stars
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