t4 polynucleotide kinase  (Millipore)


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    Name:
    Polynucleotide Kinase
    Description:
    Polynucleotide kinase catalyzes the transfer of the terminal phosphate group of ATP to the 5 hydroxyl terminus of DNA or RNA It also can catalyze the exchange of 5 terminal phosphate groups In addition the enzyme has a 3 phosphatase activity only at low pH
    Catalog Number:
    pnk-ro
    Price:
    None
    Applications:
    Use this polynucleotide kinase to: . Phosphorylate 5' ends of DNA or RNA. Label 5'-hydroxyl ends of DNA or RNA with [gamma-32P]-ATP (either by direct phosphorylation or by phosphate exchange). Remove 3'-phosphates from RNA or DNA without altering their 5' ends (at low pH only)
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    Structured Review

    Millipore t4 polynucleotide kinase
    Polynucleotide kinase catalyzes the transfer of the terminal phosphate group of ATP to the 5 hydroxyl terminus of DNA or RNA It also can catalyze the exchange of 5 terminal phosphate groups In addition the enzyme has a 3 phosphatase activity only at low pH
    https://www.bioz.com/result/t4 polynucleotide kinase/product/Millipore
    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide kinase - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    Produced:

    Article Title: Xrcc4 physically links DNA end processing by polynucleotide kinase to DNA ligation by DNA ligase IV
    Article Snippet: .. PNK and Xrcc4 recombinant proteins were produced in Escherichia coli BL21(DE3)/pLysS (Novagen), according to the manufacturer's protocol. ..

    Concentration Assay:

    Article Title: Efficient RNA pseudouridylation by eukaryotic H/ACA ribonucleoproteins requires high affinity binding and correct positioning of guide RNA
    Article Snippet: .. In short, RNA was dephosphorylated with Calf Intestinal Alkaline Phosphatase (NEB, 0.1 U/μl), rephosphorylated with T4 polynucleotide kinase (0.5 U/μl) in the presence of [γ-32 P]ATP (5–10 μCi) and contaminating [γ-32 P]ATP was removed by using a SigmaPrep spin column (Sigma-Aldrich) containing Sephadex G-25 resin or using an EZ-10 Spin Column RNA Cleanup and Concentration Kit (Bio Basic Canada). ..

    Recombinant:

    Article Title: Xrcc4 physically links DNA end processing by polynucleotide kinase to DNA ligation by DNA ligase IV
    Article Snippet: .. PNK and Xrcc4 recombinant proteins were produced in Escherichia coli BL21(DE3)/pLysS (Novagen), according to the manufacturer's protocol. ..

    Labeling:

    Article Title: The Adsorption Protein Genes of Xanthomonas campestris Filamentous Phages Determining Host Specificity
    Article Snippet: .. The primer was labeled with [γ-32 P]ATP at the 5′ end by using T4 polynucleotide kinase and purified by passage through a Spin column-10 (Sigma). .. For annealing, the labeled primer (ca.

    Article Title: Multipronged regulatory functions of a novel endonuclease (TieA) from Helicobacter pylori
    Article Snippet: .. The annealed oligonucleotides were labeled at the 5′ end with [γ32 -P] ATP (BRIT, India) using T4 polynucleotide kinase (Sigma-Aldrich) and purified with microspin-G25 columns (GE Healthcare, Sweden). ..

    Purification:

    Article Title: The Adsorption Protein Genes of Xanthomonas campestris Filamentous Phages Determining Host Specificity
    Article Snippet: .. The primer was labeled with [γ-32 P]ATP at the 5′ end by using T4 polynucleotide kinase and purified by passage through a Spin column-10 (Sigma). .. For annealing, the labeled primer (ca.

    Article Title: Multipronged regulatory functions of a novel endonuclease (TieA) from Helicobacter pylori
    Article Snippet: .. The annealed oligonucleotides were labeled at the 5′ end with [γ32 -P] ATP (BRIT, India) using T4 polynucleotide kinase (Sigma-Aldrich) and purified with microspin-G25 columns (GE Healthcare, Sweden). ..

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  • 93
    Millipore t4 polynucleotide kinase
    T4 Polynucleotide Kinase, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide kinase/product/Millipore
    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide kinase - by Bioz Stars, 2020-07
    93/100 stars
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