Structured Review

tiangen biotech co t4 ligase
T4 Ligase, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase/product/tiangen biotech co
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
t4 ligase - by Bioz Stars, 2020-05
92/100 stars

Images

Related Articles

DNA Extraction:

Article Title: Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Ganglia of Human Gastrointestinal Tract
Article Snippet: .. Five μ g of the product was separated on 2% agarose gel by electrophoresis, extracted from the gel using the Gel DNA Extraction kit (Tiangen Biotech, Beijing, China), and then subcloned into the pGM-T vector by T4 ligase (Tiangen Biotech). .. The plasmid was then transfected into Escherichia coli XLl-Blue.

Clone Assay:

Article Title: Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Article Snippet: .. The amplicon and pET-15b cloning/expression vector (Novagen) were digested by the corresponding restriction enzymes (Nde I and Bam HI) and the ligation reaction was set up at 1:1 molar ratio to insert the double-digested amplicon downstream of the vector-derived 6xHis-tag of pET15b in correct ORF using T4-ligase (Tiangen, China), according to the manufacturer’s instructions. .. The recombinant pET-15b plasmid containing LtSti1 construct was confirmed (by restriction analyses and DNA sequencing) and was transformed into Escherichia coli BL21 (DE3) for protein expression.

Article Title: Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Article Snippet: .. The amplicon and pET-15b cloning/expression vector (Novagen) were digested by the corresponding restriction enzymes ( Nde I and Bam HI) and the ligation reaction was set up at 1:1 molar ratio to insert the double-digested amplicon downstream of the vector-derived 6xHis-tag of pET15b in correct ORF using T4-ligase (Tiangen, China), according to the manufacturer’s instructions. .. The recombinant pET-15b plasmid containing LtSti1 construct was confirmed (by restriction analyses and DNA sequencing) and was transformed into Escherichia coli BL21 (DE3) for protein expression.

Article Title: Characterization of the human mucin 5AC promoter and its regulation by the histone acetyltransferase P300
Article Snippet: .. The vector and the target fragments were linked using a T4 ligase; competent DH5α cells (Tiangen Biotech Co., Ltd.) were transformed, positive clones were selected, and verification was conducted using restriction endonuclease analysis and DNA sequencing. .. The successfully constructed plasmids were named pGL-1300/+48, P2: pGL-935/+48, P3: pGL-583/+48, P4: pGL-205/+48, P5: pGL-116/+48, and P6: pGL-23/+48.

Amplification:

Article Title: Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Article Snippet: .. The amplicon and pET-15b cloning/expression vector (Novagen) were digested by the corresponding restriction enzymes (Nde I and Bam HI) and the ligation reaction was set up at 1:1 molar ratio to insert the double-digested amplicon downstream of the vector-derived 6xHis-tag of pET15b in correct ORF using T4-ligase (Tiangen, China), according to the manufacturer’s instructions. .. The recombinant pET-15b plasmid containing LtSti1 construct was confirmed (by restriction analyses and DNA sequencing) and was transformed into Escherichia coli BL21 (DE3) for protein expression.

Article Title: Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Article Snippet: .. The amplicon and pET-15b cloning/expression vector (Novagen) were digested by the corresponding restriction enzymes ( Nde I and Bam HI) and the ligation reaction was set up at 1:1 molar ratio to insert the double-digested amplicon downstream of the vector-derived 6xHis-tag of pET15b in correct ORF using T4-ligase (Tiangen, China), according to the manufacturer’s instructions. .. The recombinant pET-15b plasmid containing LtSti1 construct was confirmed (by restriction analyses and DNA sequencing) and was transformed into Escherichia coli BL21 (DE3) for protein expression.

Agarose Gel Electrophoresis:

Article Title: Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Ganglia of Human Gastrointestinal Tract
Article Snippet: .. Five μ g of the product was separated on 2% agarose gel by electrophoresis, extracted from the gel using the Gel DNA Extraction kit (Tiangen Biotech, Beijing, China), and then subcloned into the pGM-T vector by T4 ligase (Tiangen Biotech). .. The plasmid was then transfected into Escherichia coli XLl-Blue.

Ligation:

Article Title: Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Article Snippet: .. The amplicon and pET-15b cloning/expression vector (Novagen) were digested by the corresponding restriction enzymes (Nde I and Bam HI) and the ligation reaction was set up at 1:1 molar ratio to insert the double-digested amplicon downstream of the vector-derived 6xHis-tag of pET15b in correct ORF using T4-ligase (Tiangen, China), according to the manufacturer’s instructions. .. The recombinant pET-15b plasmid containing LtSti1 construct was confirmed (by restriction analyses and DNA sequencing) and was transformed into Escherichia coli BL21 (DE3) for protein expression.

Article Title: Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Article Snippet: .. The amplicon and pET-15b cloning/expression vector (Novagen) were digested by the corresponding restriction enzymes ( Nde I and Bam HI) and the ligation reaction was set up at 1:1 molar ratio to insert the double-digested amplicon downstream of the vector-derived 6xHis-tag of pET15b in correct ORF using T4-ligase (Tiangen, China), according to the manufacturer’s instructions. .. The recombinant pET-15b plasmid containing LtSti1 construct was confirmed (by restriction analyses and DNA sequencing) and was transformed into Escherichia coli BL21 (DE3) for protein expression.

Article Title: An Asp7Gly Substitution in PPARG Is Associated with Decreased Transcriptional Activation Activity
Article Snippet: .. After double digestion with Eco RI and Kpn I, ligation with T4 ligase and screening in competent Escherichia coli Top10 cells (Tiangen, Beijing, China), the vectors pEGFP-C1-PPARG-WT and pEGFP-C1-PPARG-MT were validated by double digestion and sequencing. ..

Electrophoresis:

Article Title: Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Ganglia of Human Gastrointestinal Tract
Article Snippet: .. Five μ g of the product was separated on 2% agarose gel by electrophoresis, extracted from the gel using the Gel DNA Extraction kit (Tiangen Biotech, Beijing, China), and then subcloned into the pGM-T vector by T4 ligase (Tiangen Biotech). .. The plasmid was then transfected into Escherichia coli XLl-Blue.

Sequencing:

Article Title: An Asp7Gly Substitution in PPARG Is Associated with Decreased Transcriptional Activation Activity
Article Snippet: .. After double digestion with Eco RI and Kpn I, ligation with T4 ligase and screening in competent Escherichia coli Top10 cells (Tiangen, Beijing, China), the vectors pEGFP-C1-PPARG-WT and pEGFP-C1-PPARG-MT were validated by double digestion and sequencing. ..

Positron Emission Tomography:

Article Title: Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Article Snippet: .. The amplicon and pET-15b cloning/expression vector (Novagen) were digested by the corresponding restriction enzymes (Nde I and Bam HI) and the ligation reaction was set up at 1:1 molar ratio to insert the double-digested amplicon downstream of the vector-derived 6xHis-tag of pET15b in correct ORF using T4-ligase (Tiangen, China), according to the manufacturer’s instructions. .. The recombinant pET-15b plasmid containing LtSti1 construct was confirmed (by restriction analyses and DNA sequencing) and was transformed into Escherichia coli BL21 (DE3) for protein expression.

Article Title: Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Article Snippet: .. The amplicon and pET-15b cloning/expression vector (Novagen) were digested by the corresponding restriction enzymes ( Nde I and Bam HI) and the ligation reaction was set up at 1:1 molar ratio to insert the double-digested amplicon downstream of the vector-derived 6xHis-tag of pET15b in correct ORF using T4-ligase (Tiangen, China), according to the manufacturer’s instructions. .. The recombinant pET-15b plasmid containing LtSti1 construct was confirmed (by restriction analyses and DNA sequencing) and was transformed into Escherichia coli BL21 (DE3) for protein expression.

Article Title: Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study
Article Snippet: .. The PCR product, encoding the mature lipBJ10, and plasmid pET-22b(+) were both digested with restriction enzymes Nde I and Xho I, and then the fragments were ligated with T4 ligase (TIANGEN Biotech (Beijing) Co., Ltd.) at 16 °C overnight. .. The resulting plasmid, without a signal peptide, was named pET-SigPFL00.

Article Title: Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study
Article Snippet: .. The plasmid pET-SigPFL was subsequently digested with restriction enzymes Nde I and Eco RI and then ligated with six different signal peptide nucleotide sequences at 16 °C overnight using T4 ligase. .. The resulting plasmids, each with different signal peptide, were named pET-SigPFL01, pET-SigPFL02, pET-SigPFL03, pET-SigPFL04, pET-SigPFL05 and pET-SigPFL06 (Table ).

DNA Sequencing:

Article Title: Characterization of the human mucin 5AC promoter and its regulation by the histone acetyltransferase P300
Article Snippet: .. The vector and the target fragments were linked using a T4 ligase; competent DH5α cells (Tiangen Biotech Co., Ltd.) were transformed, positive clones were selected, and verification was conducted using restriction endonuclease analysis and DNA sequencing. .. The successfully constructed plasmids were named pGL-1300/+48, P2: pGL-935/+48, P3: pGL-583/+48, P4: pGL-205/+48, P5: pGL-116/+48, and P6: pGL-23/+48.

Polymerase Chain Reaction:

Article Title: Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study
Article Snippet: .. The PCR product, encoding the mature lipBJ10, and plasmid pET-22b(+) were both digested with restriction enzymes Nde I and Xho I, and then the fragments were ligated with T4 ligase (TIANGEN Biotech (Beijing) Co., Ltd.) at 16 °C overnight. .. The resulting plasmid, without a signal peptide, was named pET-SigPFL00.

Transformation Assay:

Article Title: Characterization of the human mucin 5AC promoter and its regulation by the histone acetyltransferase P300
Article Snippet: .. The vector and the target fragments were linked using a T4 ligase; competent DH5α cells (Tiangen Biotech Co., Ltd.) were transformed, positive clones were selected, and verification was conducted using restriction endonuclease analysis and DNA sequencing. .. The successfully constructed plasmids were named pGL-1300/+48, P2: pGL-935/+48, P3: pGL-583/+48, P4: pGL-205/+48, P5: pGL-116/+48, and P6: pGL-23/+48.

Plasmid Preparation:

Article Title: Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Article Snippet: .. The amplicon and pET-15b cloning/expression vector (Novagen) were digested by the corresponding restriction enzymes (Nde I and Bam HI) and the ligation reaction was set up at 1:1 molar ratio to insert the double-digested amplicon downstream of the vector-derived 6xHis-tag of pET15b in correct ORF using T4-ligase (Tiangen, China), according to the manufacturer’s instructions. .. The recombinant pET-15b plasmid containing LtSti1 construct was confirmed (by restriction analyses and DNA sequencing) and was transformed into Escherichia coli BL21 (DE3) for protein expression.

Article Title: Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Article Snippet: .. The amplicon and pET-15b cloning/expression vector (Novagen) were digested by the corresponding restriction enzymes ( Nde I and Bam HI) and the ligation reaction was set up at 1:1 molar ratio to insert the double-digested amplicon downstream of the vector-derived 6xHis-tag of pET15b in correct ORF using T4-ligase (Tiangen, China), according to the manufacturer’s instructions. .. The recombinant pET-15b plasmid containing LtSti1 construct was confirmed (by restriction analyses and DNA sequencing) and was transformed into Escherichia coli BL21 (DE3) for protein expression.

Article Title: Characterization of the human mucin 5AC promoter and its regulation by the histone acetyltransferase P300
Article Snippet: .. The vector and the target fragments were linked using a T4 ligase; competent DH5α cells (Tiangen Biotech Co., Ltd.) were transformed, positive clones were selected, and verification was conducted using restriction endonuclease analysis and DNA sequencing. .. The successfully constructed plasmids were named pGL-1300/+48, P2: pGL-935/+48, P3: pGL-583/+48, P4: pGL-205/+48, P5: pGL-116/+48, and P6: pGL-23/+48.

Article Title: Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study
Article Snippet: .. The PCR product, encoding the mature lipBJ10, and plasmid pET-22b(+) were both digested with restriction enzymes Nde I and Xho I, and then the fragments were ligated with T4 ligase (TIANGEN Biotech (Beijing) Co., Ltd.) at 16 °C overnight. .. The resulting plasmid, without a signal peptide, was named pET-SigPFL00.

Article Title: Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study
Article Snippet: .. The plasmid pET-SigPFL was subsequently digested with restriction enzymes Nde I and Eco RI and then ligated with six different signal peptide nucleotide sequences at 16 °C overnight using T4 ligase. .. The resulting plasmids, each with different signal peptide, were named pET-SigPFL01, pET-SigPFL02, pET-SigPFL03, pET-SigPFL04, pET-SigPFL05 and pET-SigPFL06 (Table ).

Article Title: Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Ganglia of Human Gastrointestinal Tract
Article Snippet: .. Five μ g of the product was separated on 2% agarose gel by electrophoresis, extracted from the gel using the Gel DNA Extraction kit (Tiangen Biotech, Beijing, China), and then subcloned into the pGM-T vector by T4 ligase (Tiangen Biotech). .. The plasmid was then transfected into Escherichia coli XLl-Blue.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    tiangen biotech co t4 dna ligase
    T4 Dna Ligase, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/tiangen biotech co
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    Image Search Results