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TransGen biotech co t4 ligase
T4 Ligase, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase/product/TransGen biotech co
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t4 ligase - by Bioz Stars, 2020-05
92/100 stars

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Article Title:
Article Snippet: Thus, the primers p8 and p9 were used for a round PCR2 and the product was ligated with T4 ligase to create the plasmid pNZ8048-SPusp45 -promtg in which the recombinant pro-MTG-6His will be initiated by the nisin-inducible promoter PnisA ( ).

Article Title:
Article Snippet: Briefly, 2 μl (300 ng/μl) of gDNA of P. litchii (wild-type isolate 38) was digested with 1 μl of Eco R I (Thermo Scitific, Shanghai, China) in a total volume of 20 μl at 37 °C for overnight; the enzyme activity was inactivated by heating at 65 °C for 20 min. Circularization was conducted in a total reaction volume of 200 μl containing 4 μl of digesting DNA products, 5 μl of T4 ligase (TransGen Biotech, Beijing, China), 20 μl of 10× T4 DNA ligase buffer (TransGen Biotech, Beijing, China), and 171 μl of ddH2 O.

Article Title:
Article Snippet: PCR products were then digested by DpnI (Thermofisher, Waltham, MA) and ligated with T4 ligase (TransGen Biotech, Beijing, China), thereby substituting the eGFP tag sequence with the HA sequence.

Article Title:
Article Snippet: Then the resulting two fragments Bam HI- pNZ8048-mature-mtg-Nco I and Nco I- SPusp45 -proregion-Bam HI were digested using Nco I and Bam HI and ligated using T4 ligase as above (TransGen Biotech, Beijing, China), generating the expression vector pNZ8048-SPusp45 (K2A) -promtg .

Article Title:
Article Snippet: T4 ligase was purchased from TransGen Biotech.

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    TransGen biotech co t4 dna ligase
    T4 Dna Ligase, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/TransGen biotech co
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-05
    94/100 stars
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