Structured Review

Toyobo t4 ligase
T4 Ligase, supplied by Toyobo, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase/product/Toyobo
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
t4 ligase - by Bioz Stars, 2020-05
93/100 stars

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Related Articles

Clone Assay:

Article Title: Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs invitro
Article Snippet: .. Construction of NSs over-expression plasmid Open reading frame(ORF) encoding NSs was amplified by reverse transcription-PCR(RT-PCR) from SFTSV genomic RNA, and two restriction enzymes Xhol and PremI (New England Biolabs, USA) were used to cloned the NSs segment into expression vector pc-DNA3.1 with three tag (Flag, His, StrepII) using T4 ligase (Toyobo, Japan). .. The primers for SFTSV NSs plasmid construction were: forward: 5’-TTAGACCTCCTTCGGGAGGTCA-3’ ; reverse: 5’-TGGAGCATTTGCTCAG CGACAT-3’ .

Amplification:

Article Title: TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism
Article Snippet: .. Amplified fragments were treated with T4 polynucleotide kinase (Takara, Japan), ligated by T4 ligase (ToYoBo, Japan) with pcDNA3.1 (+) (Life Technologies, USA), and pretreated with Eco R V-HF (NEB, USA) and CIP (Takara, Japan). ..

Article Title: Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs invitro
Article Snippet: .. Construction of NSs over-expression plasmid Open reading frame(ORF) encoding NSs was amplified by reverse transcription-PCR(RT-PCR) from SFTSV genomic RNA, and two restriction enzymes Xhol and PremI (New England Biolabs, USA) were used to cloned the NSs segment into expression vector pc-DNA3.1 with three tag (Flag, His, StrepII) using T4 ligase (Toyobo, Japan). .. The primers for SFTSV NSs plasmid construction were: forward: 5’-TTAGACCTCCTTCGGGAGGTCA-3’ ; reverse: 5’-TGGAGCATTTGCTCAG CGACAT-3’ .

Inverse PCR:

Article Title: Multiple invasions of an infectious retrovirus in cat genomes
Article Snippet: .. Inverse PCR Genomic DNA was digested with Eco RI or Sph I and 100 ng of digested genomes were ligated by T4 ligase (TOYOBO, Osaka, Japan) at 16°C overnight. .. Ligated DNA was amplified with specific primers listed in using PrimeSTAR GXL polymerase (TaKaRa).

Article Title: Idaten Is a New Cold-Inducible Transposon of Volvox carteri That Can Be Used for Tagging Developmentally Important Genes
Article Snippet: .. After self-ligation of 9.5 ng of the extracted DNA with T4 ligase (TOYOBO) at 37° for 1 hr in a total volume of 450 μl, 5 μl of the ligation mixture were used as a template for inverse PCR in a total volume of 50 μl. .. The primers used were IF02 (5′-TGC TGA ATC TGC CAA GTA GGC TCT) and IR02 (5′-TGG CTG TTA TGC CAC GTT GAC AAC).

Ligation:

Article Title: Idaten Is a New Cold-Inducible Transposon of Volvox carteri That Can Be Used for Tagging Developmentally Important Genes
Article Snippet: .. After self-ligation of 9.5 ng of the extracted DNA with T4 ligase (TOYOBO) at 37° for 1 hr in a total volume of 450 μl, 5 μl of the ligation mixture were used as a template for inverse PCR in a total volume of 50 μl. .. The primers used were IF02 (5′-TGC TGA ATC TGC CAA GTA GGC TCT) and IR02 (5′-TGG CTG TTA TGC CAC GTT GAC AAC).

Construct:

Article Title: Construction of Corynebacterium glutamicum cells as containers encapsulating dsRNA overexpressed for agricultural pest control
Article Snippet: .. Finally, this DNA fragment and pVH2-KX-Pf1rev were treated with restriction enzymes Xho I and Kpn I, and the DNA fragments were ligated with T4 ligase (Toyobo, Osaka, Japan) to construct plasmid pVH2-HvIap-1 (Fig. ). .. Thus, an expression system for diap1* -dsRNA was constructed in which two F1 promoters faced each other across the partial diap1 coding fragment.

Expressing:

Article Title: Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs invitro
Article Snippet: .. Construction of NSs over-expression plasmid Open reading frame(ORF) encoding NSs was amplified by reverse transcription-PCR(RT-PCR) from SFTSV genomic RNA, and two restriction enzymes Xhol and PremI (New England Biolabs, USA) were used to cloned the NSs segment into expression vector pc-DNA3.1 with three tag (Flag, His, StrepII) using T4 ligase (Toyobo, Japan). .. The primers for SFTSV NSs plasmid construction were: forward: 5’-TTAGACCTCCTTCGGGAGGTCA-3’ ; reverse: 5’-TGGAGCATTTGCTCAG CGACAT-3’ .

Over Expression:

Article Title: Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs invitro
Article Snippet: .. Construction of NSs over-expression plasmid Open reading frame(ORF) encoding NSs was amplified by reverse transcription-PCR(RT-PCR) from SFTSV genomic RNA, and two restriction enzymes Xhol and PremI (New England Biolabs, USA) were used to cloned the NSs segment into expression vector pc-DNA3.1 with three tag (Flag, His, StrepII) using T4 ligase (Toyobo, Japan). .. The primers for SFTSV NSs plasmid construction were: forward: 5’-TTAGACCTCCTTCGGGAGGTCA-3’ ; reverse: 5’-TGGAGCATTTGCTCAG CGACAT-3’ .

Plasmid Preparation:

Article Title: Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs invitro
Article Snippet: .. Construction of NSs over-expression plasmid Open reading frame(ORF) encoding NSs was amplified by reverse transcription-PCR(RT-PCR) from SFTSV genomic RNA, and two restriction enzymes Xhol and PremI (New England Biolabs, USA) were used to cloned the NSs segment into expression vector pc-DNA3.1 with three tag (Flag, His, StrepII) using T4 ligase (Toyobo, Japan). .. The primers for SFTSV NSs plasmid construction were: forward: 5’-TTAGACCTCCTTCGGGAGGTCA-3’ ; reverse: 5’-TGGAGCATTTGCTCAG CGACAT-3’ .

Article Title: Construction of Corynebacterium glutamicum cells as containers encapsulating dsRNA overexpressed for agricultural pest control
Article Snippet: .. Finally, this DNA fragment and pVH2-KX-Pf1rev were treated with restriction enzymes Xho I and Kpn I, and the DNA fragments were ligated with T4 ligase (Toyobo, Osaka, Japan) to construct plasmid pVH2-HvIap-1 (Fig. ). .. Thus, an expression system for diap1* -dsRNA was constructed in which two F1 promoters faced each other across the partial diap1 coding fragment.

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  • 94
    Toyobo t4 dna ligase
    T4 Dna Ligase, supplied by Toyobo, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/Toyobo
    Average 94 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    93
    Toyobo t4 ligase
    T4 Ligase, supplied by Toyobo, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase/product/Toyobo
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t4 ligase - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

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