Structured Review

Kaneka Corp t4 ligase
T4 Ligase, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase/product/Kaneka Corp
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t4 ligase - by Bioz Stars, 2020-05
92/100 stars

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Polymerase Chain Reaction:

Article Title: Immune Response to Chlamydophila abortus POMP91B Protein in the Context of Different Pathogen Associated Molecular Patterns (PAMP); Role of Antigen in the Orientation of Immune Response
Article Snippet: .. PCR products were purified, digested by the two restriction enzymes Nde I and Bam HI and ligated during 16 h at 4 °C with T4 ligase (Eurogentec) into the expression vector pET15b (Novagen) pre-digested with Nde I and Bam HI. .. This plasmid encodes six histidines tagged to the N -terminus of the expressed recombinant protein to be purified.

Expressing:

Article Title: Immune Response to Chlamydophila abortus POMP91B Protein in the Context of Different Pathogen Associated Molecular Patterns (PAMP); Role of Antigen in the Orientation of Immune Response
Article Snippet: .. PCR products were purified, digested by the two restriction enzymes Nde I and Bam HI and ligated during 16 h at 4 °C with T4 ligase (Eurogentec) into the expression vector pET15b (Novagen) pre-digested with Nde I and Bam HI. .. This plasmid encodes six histidines tagged to the N -terminus of the expressed recombinant protein to be purified.

Plasmid Preparation:

Article Title: Immune Response to Chlamydophila abortus POMP91B Protein in the Context of Different Pathogen Associated Molecular Patterns (PAMP); Role of Antigen in the Orientation of Immune Response
Article Snippet: .. PCR products were purified, digested by the two restriction enzymes Nde I and Bam HI and ligated during 16 h at 4 °C with T4 ligase (Eurogentec) into the expression vector pET15b (Novagen) pre-digested with Nde I and Bam HI. .. This plasmid encodes six histidines tagged to the N -terminus of the expressed recombinant protein to be purified.

Purification:

Article Title: Immune Response to Chlamydophila abortus POMP91B Protein in the Context of Different Pathogen Associated Molecular Patterns (PAMP); Role of Antigen in the Orientation of Immune Response
Article Snippet: .. PCR products were purified, digested by the two restriction enzymes Nde I and Bam HI and ligated during 16 h at 4 °C with T4 ligase (Eurogentec) into the expression vector pET15b (Novagen) pre-digested with Nde I and Bam HI. .. This plasmid encodes six histidines tagged to the N -terminus of the expressed recombinant protein to be purified.