Structured Review

DNA-Gdansk t4 ligase
T4 Ligase, supplied by DNA-Gdansk, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase/product/DNA-Gdansk
Average 90 stars, based on 3 article reviews
Price from $9.99 to $1999.99
t4 ligase - by Bioz Stars, 2020-05
90/100 stars

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Related Articles

Sequencing:

Article Title: Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy
Article Snippet: .. Sequencing reaction mix contained 1 × phi29 reaction buffer, 250 nM ATP (Fermentas), 0.2 mg ml−1 BSA, 100 nM sequencing library each and 100 mU μl−1 T4 ligase (DNA GDansk). .. The reaction was incubated at room temperature (22 °C) for 1 h. The reaction was stopped by removing the reaction mix and washing three times in PBS-Tween 0.05%.

Incubation:

Article Title: Sensitive and inexpensive digital DNA analysis by microfluidic enrichment of rolling circle amplified single-molecules
Article Snippet: .. A ligation mix, composed of 10 nM PA padlock probe, 30 nM PA synthetic target, T4 ligase reaction buffer (66 mM Tris-HCl (pH 7.5), 10 mM DTT, 10 mM MgCl2 , DNA-Gdansk), 0.2 μg/μl BSA, 0.68 mM ATP (DNA-Gdansk) and 1 U T4 ligase (DNA-Gdansk) in 100 μl reaction volume, was incubated at 37°C for 20 min. .. The reaction was heat inactivated at 65°C for 2 min. Ligated circles were diluted and amplified by a target- primed RCA reaction composed of 100 pM ligated circles (concentration estimated on the initial padlock probe concentration), 0.2 μg/μl BSA, ϕ29 polymerase reaction buffer (33 mM Tris-acetate pH-7.9, 10 mM Mg-acetate, 66 mM K-acetate, 0.1% (v/v), Tween 20, 1 mM DTT, Thermo Scientific), 125 μM dNTPs (DNA Gdansk) and 0.2 U/μl ϕ29 polymerase (Olink, Sweden), which was incubated at 37°C for 60 min and 65°C for 2 min for enzyme heat inactivation.

Ligation:

Article Title: Next-generation bis-locked nucleic acids with stacking linker and 2′-glycylamino-LNA show enhanced DNA invasion into supercoiled duplexes
Article Snippet: .. Padlock probe ligation on the displaced DNA strand was performed in 1xT4 ligase buffer (DNA Gdansk) with 2 μM padlock probe, 1 mM ATP (DNA Gdansk), 0.25 M NaCl, 0.1 U/μl T4 Ligase (DNA Gdansk) for 2 h at 25°C. .. Slides were washed twice in 1xBuffer-B for 5 min at 25°C and ligated padlock probes were amplified by RCA.

Article Title: Sensitive and inexpensive digital DNA analysis by microfluidic enrichment of rolling circle amplified single-molecules
Article Snippet: .. A ligation mix, composed of 10 nM PA padlock probe, 30 nM PA synthetic target, T4 ligase reaction buffer (66 mM Tris-HCl (pH 7.5), 10 mM DTT, 10 mM MgCl2 , DNA-Gdansk), 0.2 μg/μl BSA, 0.68 mM ATP (DNA-Gdansk) and 1 U T4 ligase (DNA-Gdansk) in 100 μl reaction volume, was incubated at 37°C for 20 min. .. The reaction was heat inactivated at 65°C for 2 min. Ligated circles were diluted and amplified by a target- primed RCA reaction composed of 100 pM ligated circles (concentration estimated on the initial padlock probe concentration), 0.2 μg/μl BSA, ϕ29 polymerase reaction buffer (33 mM Tris-acetate pH-7.9, 10 mM Mg-acetate, 66 mM K-acetate, 0.1% (v/v), Tween 20, 1 mM DTT, Thermo Scientific), 125 μM dNTPs (DNA Gdansk) and 0.2 U/μl ϕ29 polymerase (Olink, Sweden), which was incubated at 37°C for 60 min and 65°C for 2 min for enzyme heat inactivation.

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  • 90
    DNA-Gdansk t4 ligase
    T4 Ligase, supplied by DNA-Gdansk, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase/product/DNA-Gdansk
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    t4 ligase - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    91
    DNA-Gdansk t4 ligase reaction buffer
    T4 Ligase Reaction Buffer, supplied by DNA-Gdansk, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase reaction buffer/product/DNA-Gdansk
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 ligase reaction buffer - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

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