t4 ligase prepared  (Millipore)


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    Millipore t4 ligase prepared
    T4 Ligase Prepared, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase prepared/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 ligase prepared - by Bioz Stars, 2020-05
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Molecular phylogenic location of the Plagiorchis muris (Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome C oxidase subunit I
    Article Snippet: .. Amplified PCR products were extracted and purified using a gel extraction kit and a PCR purification kit (QIAGEN Co., Germany) and were cloned into a pT7Blue Perfectly Blunt cloning vector using T4 DNA ligase and transformed into E.coli Nova Blue competent cells, according to the supplier's protocol (Novagen Co. USA). .. Positive recombinant clones were picked, and grown in 2 ml of LB broth (in the presence of 50 g/ml ampicillin) overnight at 37℃.

    Article Title: Flagellar Radial Spoke Protein 2 Is a Calmodulin Binding Protein Required for Motility in Chlamydomonas reinhardtii
    Article Snippet: .. The three fragments were ligated with T4 DNA ligase and cloned into the Nco I and Sal I sites in the expression vector pET28a (Novagen). .. Based upon sequencing and Western blotting with antibodies to RSP2, the construct expressed the authentic RSP2 (see Results and Discussion).

    Amplification:

    Article Title: Antibiotic binding of STY3178, a yfdX protein from Salmonella Typhi
    Article Snippet: .. The amplified PCR product is then inserted into TA vector using T4 DNA ligase and transformed into Top10 E.coli cells (Novagen). .. The transformed bacteria with the desired gene are confirmed by screening the blue/white colonies followed by colony PCR.

    Article Title: Nucleoside analog studies indicate mechanistic differences between RNA-editing adenosine deaminases
    Article Snippet: .. Reagents for in vitro transcription, in vitro editing, PCR amplification, radiolabeling and preparation of RNAs for splinted ligation were purchased from: Perkin-Elmer Life Sciences: γ-[32 P]ATP (6000 Ci/mmol); GE Healthcare: MicroSpin G-25 columns; Promega: RQ1 RNase free DNase, yeast tRNAPhe , RNasin, Access RT-PCR kit, ribonucleotides; New England Biolabs: T4 polynucleotide kinase, T4 DNA ligase, BamHI, acyclonucleotides, deoxynucleotides, VentR® exo(−) DNA Polymerase, RNase Inhibitor, SacI, XbaI, Quick Ligase Kit, DNase I; Sigma Aldrich: Nuclease P1, glycerol and phenol:chloroform; Life Technologies: DNA oligonucleotides; University of Utah DNA/Core Peptide Facility: RNA oligonucleotides; Axxora: 7-deazaadenosine 5′-O -triphosphate; Glen Research: 2′-O -methyladenosine, 8-aza-7-deazaadenosine and 7-deazaadenosine ribonucleoside phosphoramidites; Qiagen: Gel Extraction Kit, PCR Purification Kit. .. Radioactive gels and TLC plates were imaged using storage phosphor imaging plates from Molecular Dynamics on a Molecular Dynamics 9400 Typhoon phosphorimager.

    Article Title: Molecular phylogenic location of the Plagiorchis muris (Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome C oxidase subunit I
    Article Snippet: .. Amplified PCR products were extracted and purified using a gel extraction kit and a PCR purification kit (QIAGEN Co., Germany) and were cloned into a pT7Blue Perfectly Blunt cloning vector using T4 DNA ligase and transformed into E.coli Nova Blue competent cells, according to the supplier's protocol (Novagen Co. USA). .. Positive recombinant clones were picked, and grown in 2 ml of LB broth (in the presence of 50 g/ml ampicillin) overnight at 37℃.

    Agarose Gel Electrophoresis:

    Article Title: Signaling mechanism by theStaphylococcus aureus two-component system LytSR: role of acetyl phosphate in bypassing the cell membrane electrical potential sensor LytS
    Article Snippet: .. The digestion products were gel purified from 1% agarose gel using the QIAquick Gel Extraction Kit (Qiagen) and subjected to ligation using T4 DNA ligase (NOVAGEN). .. The subsequent construct was referred to aspET26b::lytR and was used to transformE. coli NovaBlue cells for further amplification.

    In Vitro:

    Article Title: Nucleoside analog studies indicate mechanistic differences between RNA-editing adenosine deaminases
    Article Snippet: .. Reagents for in vitro transcription, in vitro editing, PCR amplification, radiolabeling and preparation of RNAs for splinted ligation were purchased from: Perkin-Elmer Life Sciences: γ-[32 P]ATP (6000 Ci/mmol); GE Healthcare: MicroSpin G-25 columns; Promega: RQ1 RNase free DNase, yeast tRNAPhe , RNasin, Access RT-PCR kit, ribonucleotides; New England Biolabs: T4 polynucleotide kinase, T4 DNA ligase, BamHI, acyclonucleotides, deoxynucleotides, VentR® exo(−) DNA Polymerase, RNase Inhibitor, SacI, XbaI, Quick Ligase Kit, DNase I; Sigma Aldrich: Nuclease P1, glycerol and phenol:chloroform; Life Technologies: DNA oligonucleotides; University of Utah DNA/Core Peptide Facility: RNA oligonucleotides; Axxora: 7-deazaadenosine 5′-O -triphosphate; Glen Research: 2′-O -methyladenosine, 8-aza-7-deazaadenosine and 7-deazaadenosine ribonucleoside phosphoramidites; Qiagen: Gel Extraction Kit, PCR Purification Kit. .. Radioactive gels and TLC plates were imaged using storage phosphor imaging plates from Molecular Dynamics on a Molecular Dynamics 9400 Typhoon phosphorimager.

    Ligation:

    Article Title: Nucleoside analog studies indicate mechanistic differences between RNA-editing adenosine deaminases
    Article Snippet: .. Reagents for in vitro transcription, in vitro editing, PCR amplification, radiolabeling and preparation of RNAs for splinted ligation were purchased from: Perkin-Elmer Life Sciences: γ-[32 P]ATP (6000 Ci/mmol); GE Healthcare: MicroSpin G-25 columns; Promega: RQ1 RNase free DNase, yeast tRNAPhe , RNasin, Access RT-PCR kit, ribonucleotides; New England Biolabs: T4 polynucleotide kinase, T4 DNA ligase, BamHI, acyclonucleotides, deoxynucleotides, VentR® exo(−) DNA Polymerase, RNase Inhibitor, SacI, XbaI, Quick Ligase Kit, DNase I; Sigma Aldrich: Nuclease P1, glycerol and phenol:chloroform; Life Technologies: DNA oligonucleotides; University of Utah DNA/Core Peptide Facility: RNA oligonucleotides; Axxora: 7-deazaadenosine 5′-O -triphosphate; Glen Research: 2′-O -methyladenosine, 8-aza-7-deazaadenosine and 7-deazaadenosine ribonucleoside phosphoramidites; Qiagen: Gel Extraction Kit, PCR Purification Kit. .. Radioactive gels and TLC plates were imaged using storage phosphor imaging plates from Molecular Dynamics on a Molecular Dynamics 9400 Typhoon phosphorimager.

    Article Title: Signaling mechanism by theStaphylococcus aureus two-component system LytSR: role of acetyl phosphate in bypassing the cell membrane electrical potential sensor LytS
    Article Snippet: .. The digestion products were gel purified from 1% agarose gel using the QIAquick Gel Extraction Kit (Qiagen) and subjected to ligation using T4 DNA ligase (NOVAGEN). .. The subsequent construct was referred to aspET26b::lytR and was used to transformE. coli NovaBlue cells for further amplification.

    Purification:

    Article Title: Nucleoside analog studies indicate mechanistic differences between RNA-editing adenosine deaminases
    Article Snippet: .. Reagents for in vitro transcription, in vitro editing, PCR amplification, radiolabeling and preparation of RNAs for splinted ligation were purchased from: Perkin-Elmer Life Sciences: γ-[32 P]ATP (6000 Ci/mmol); GE Healthcare: MicroSpin G-25 columns; Promega: RQ1 RNase free DNase, yeast tRNAPhe , RNasin, Access RT-PCR kit, ribonucleotides; New England Biolabs: T4 polynucleotide kinase, T4 DNA ligase, BamHI, acyclonucleotides, deoxynucleotides, VentR® exo(−) DNA Polymerase, RNase Inhibitor, SacI, XbaI, Quick Ligase Kit, DNase I; Sigma Aldrich: Nuclease P1, glycerol and phenol:chloroform; Life Technologies: DNA oligonucleotides; University of Utah DNA/Core Peptide Facility: RNA oligonucleotides; Axxora: 7-deazaadenosine 5′-O -triphosphate; Glen Research: 2′-O -methyladenosine, 8-aza-7-deazaadenosine and 7-deazaadenosine ribonucleoside phosphoramidites; Qiagen: Gel Extraction Kit, PCR Purification Kit. .. Radioactive gels and TLC plates were imaged using storage phosphor imaging plates from Molecular Dynamics on a Molecular Dynamics 9400 Typhoon phosphorimager.

    Article Title: Molecular phylogenic location of the Plagiorchis muris (Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome C oxidase subunit I
    Article Snippet: .. Amplified PCR products were extracted and purified using a gel extraction kit and a PCR purification kit (QIAGEN Co., Germany) and were cloned into a pT7Blue Perfectly Blunt cloning vector using T4 DNA ligase and transformed into E.coli Nova Blue competent cells, according to the supplier's protocol (Novagen Co. USA). .. Positive recombinant clones were picked, and grown in 2 ml of LB broth (in the presence of 50 g/ml ampicillin) overnight at 37℃.

    Article Title: Signaling mechanism by theStaphylococcus aureus two-component system LytSR: role of acetyl phosphate in bypassing the cell membrane electrical potential sensor LytS
    Article Snippet: .. The digestion products were gel purified from 1% agarose gel using the QIAquick Gel Extraction Kit (Qiagen) and subjected to ligation using T4 DNA ligase (NOVAGEN). .. The subsequent construct was referred to aspET26b::lytR and was used to transformE. coli NovaBlue cells for further amplification.

    Polymerase Chain Reaction:

    Article Title: Antibiotic binding of STY3178, a yfdX protein from Salmonella Typhi
    Article Snippet: .. The amplified PCR product is then inserted into TA vector using T4 DNA ligase and transformed into Top10 E.coli cells (Novagen). .. The transformed bacteria with the desired gene are confirmed by screening the blue/white colonies followed by colony PCR.

    Article Title: Nucleoside analog studies indicate mechanistic differences between RNA-editing adenosine deaminases
    Article Snippet: .. Reagents for in vitro transcription, in vitro editing, PCR amplification, radiolabeling and preparation of RNAs for splinted ligation were purchased from: Perkin-Elmer Life Sciences: γ-[32 P]ATP (6000 Ci/mmol); GE Healthcare: MicroSpin G-25 columns; Promega: RQ1 RNase free DNase, yeast tRNAPhe , RNasin, Access RT-PCR kit, ribonucleotides; New England Biolabs: T4 polynucleotide kinase, T4 DNA ligase, BamHI, acyclonucleotides, deoxynucleotides, VentR® exo(−) DNA Polymerase, RNase Inhibitor, SacI, XbaI, Quick Ligase Kit, DNase I; Sigma Aldrich: Nuclease P1, glycerol and phenol:chloroform; Life Technologies: DNA oligonucleotides; University of Utah DNA/Core Peptide Facility: RNA oligonucleotides; Axxora: 7-deazaadenosine 5′-O -triphosphate; Glen Research: 2′-O -methyladenosine, 8-aza-7-deazaadenosine and 7-deazaadenosine ribonucleoside phosphoramidites; Qiagen: Gel Extraction Kit, PCR Purification Kit. .. Radioactive gels and TLC plates were imaged using storage phosphor imaging plates from Molecular Dynamics on a Molecular Dynamics 9400 Typhoon phosphorimager.

    Article Title: Molecular phylogenic location of the Plagiorchis muris (Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome C oxidase subunit I
    Article Snippet: .. Amplified PCR products were extracted and purified using a gel extraction kit and a PCR purification kit (QIAGEN Co., Germany) and were cloned into a pT7Blue Perfectly Blunt cloning vector using T4 DNA ligase and transformed into E.coli Nova Blue competent cells, according to the supplier's protocol (Novagen Co. USA). .. Positive recombinant clones were picked, and grown in 2 ml of LB broth (in the presence of 50 g/ml ampicillin) overnight at 37℃.

    Radioactivity:

    Article Title: Nucleoside analog studies indicate mechanistic differences between RNA-editing adenosine deaminases
    Article Snippet: .. Reagents for in vitro transcription, in vitro editing, PCR amplification, radiolabeling and preparation of RNAs for splinted ligation were purchased from: Perkin-Elmer Life Sciences: γ-[32 P]ATP (6000 Ci/mmol); GE Healthcare: MicroSpin G-25 columns; Promega: RQ1 RNase free DNase, yeast tRNAPhe , RNasin, Access RT-PCR kit, ribonucleotides; New England Biolabs: T4 polynucleotide kinase, T4 DNA ligase, BamHI, acyclonucleotides, deoxynucleotides, VentR® exo(−) DNA Polymerase, RNase Inhibitor, SacI, XbaI, Quick Ligase Kit, DNase I; Sigma Aldrich: Nuclease P1, glycerol and phenol:chloroform; Life Technologies: DNA oligonucleotides; University of Utah DNA/Core Peptide Facility: RNA oligonucleotides; Axxora: 7-deazaadenosine 5′-O -triphosphate; Glen Research: 2′-O -methyladenosine, 8-aza-7-deazaadenosine and 7-deazaadenosine ribonucleoside phosphoramidites; Qiagen: Gel Extraction Kit, PCR Purification Kit. .. Radioactive gels and TLC plates were imaged using storage phosphor imaging plates from Molecular Dynamics on a Molecular Dynamics 9400 Typhoon phosphorimager.

    Expressing:

    Article Title: Flagellar Radial Spoke Protein 2 Is a Calmodulin Binding Protein Required for Motility in Chlamydomonas reinhardtii
    Article Snippet: .. The three fragments were ligated with T4 DNA ligase and cloned into the Nco I and Sal I sites in the expression vector pET28a (Novagen). .. Based upon sequencing and Western blotting with antibodies to RSP2, the construct expressed the authentic RSP2 (see Results and Discussion).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Nucleoside analog studies indicate mechanistic differences between RNA-editing adenosine deaminases
    Article Snippet: .. Reagents for in vitro transcription, in vitro editing, PCR amplification, radiolabeling and preparation of RNAs for splinted ligation were purchased from: Perkin-Elmer Life Sciences: γ-[32 P]ATP (6000 Ci/mmol); GE Healthcare: MicroSpin G-25 columns; Promega: RQ1 RNase free DNase, yeast tRNAPhe , RNasin, Access RT-PCR kit, ribonucleotides; New England Biolabs: T4 polynucleotide kinase, T4 DNA ligase, BamHI, acyclonucleotides, deoxynucleotides, VentR® exo(−) DNA Polymerase, RNase Inhibitor, SacI, XbaI, Quick Ligase Kit, DNase I; Sigma Aldrich: Nuclease P1, glycerol and phenol:chloroform; Life Technologies: DNA oligonucleotides; University of Utah DNA/Core Peptide Facility: RNA oligonucleotides; Axxora: 7-deazaadenosine 5′-O -triphosphate; Glen Research: 2′-O -methyladenosine, 8-aza-7-deazaadenosine and 7-deazaadenosine ribonucleoside phosphoramidites; Qiagen: Gel Extraction Kit, PCR Purification Kit. .. Radioactive gels and TLC plates were imaged using storage phosphor imaging plates from Molecular Dynamics on a Molecular Dynamics 9400 Typhoon phosphorimager.

    Gel Extraction:

    Article Title: Nucleoside analog studies indicate mechanistic differences between RNA-editing adenosine deaminases
    Article Snippet: .. Reagents for in vitro transcription, in vitro editing, PCR amplification, radiolabeling and preparation of RNAs for splinted ligation were purchased from: Perkin-Elmer Life Sciences: γ-[32 P]ATP (6000 Ci/mmol); GE Healthcare: MicroSpin G-25 columns; Promega: RQ1 RNase free DNase, yeast tRNAPhe , RNasin, Access RT-PCR kit, ribonucleotides; New England Biolabs: T4 polynucleotide kinase, T4 DNA ligase, BamHI, acyclonucleotides, deoxynucleotides, VentR® exo(−) DNA Polymerase, RNase Inhibitor, SacI, XbaI, Quick Ligase Kit, DNase I; Sigma Aldrich: Nuclease P1, glycerol and phenol:chloroform; Life Technologies: DNA oligonucleotides; University of Utah DNA/Core Peptide Facility: RNA oligonucleotides; Axxora: 7-deazaadenosine 5′-O -triphosphate; Glen Research: 2′-O -methyladenosine, 8-aza-7-deazaadenosine and 7-deazaadenosine ribonucleoside phosphoramidites; Qiagen: Gel Extraction Kit, PCR Purification Kit. .. Radioactive gels and TLC plates were imaged using storage phosphor imaging plates from Molecular Dynamics on a Molecular Dynamics 9400 Typhoon phosphorimager.

    Article Title: Molecular phylogenic location of the Plagiorchis muris (Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome C oxidase subunit I
    Article Snippet: .. Amplified PCR products were extracted and purified using a gel extraction kit and a PCR purification kit (QIAGEN Co., Germany) and were cloned into a pT7Blue Perfectly Blunt cloning vector using T4 DNA ligase and transformed into E.coli Nova Blue competent cells, according to the supplier's protocol (Novagen Co. USA). .. Positive recombinant clones were picked, and grown in 2 ml of LB broth (in the presence of 50 g/ml ampicillin) overnight at 37℃.

    Article Title: Signaling mechanism by theStaphylococcus aureus two-component system LytSR: role of acetyl phosphate in bypassing the cell membrane electrical potential sensor LytS
    Article Snippet: .. The digestion products were gel purified from 1% agarose gel using the QIAquick Gel Extraction Kit (Qiagen) and subjected to ligation using T4 DNA ligase (NOVAGEN). .. The subsequent construct was referred to aspET26b::lytR and was used to transformE. coli NovaBlue cells for further amplification.

    Transformation Assay:

    Article Title: Antibiotic binding of STY3178, a yfdX protein from Salmonella Typhi
    Article Snippet: .. The amplified PCR product is then inserted into TA vector using T4 DNA ligase and transformed into Top10 E.coli cells (Novagen). .. The transformed bacteria with the desired gene are confirmed by screening the blue/white colonies followed by colony PCR.

    Article Title: Molecular phylogenic location of the Plagiorchis muris (Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome C oxidase subunit I
    Article Snippet: .. Amplified PCR products were extracted and purified using a gel extraction kit and a PCR purification kit (QIAGEN Co., Germany) and were cloned into a pT7Blue Perfectly Blunt cloning vector using T4 DNA ligase and transformed into E.coli Nova Blue competent cells, according to the supplier's protocol (Novagen Co. USA). .. Positive recombinant clones were picked, and grown in 2 ml of LB broth (in the presence of 50 g/ml ampicillin) overnight at 37℃.

    other:

    Article Title: Molecular and Chemical Analysis of the Lipopolysaccharide from Aeromonas hydrophila Strain AH-1 (Serotype O11)
    Article Snippet: DNA restriction endonucleases, T4 DNA ligase, E. coli DNA polymerase (Klenow fragment), and al kaline phosphatase were used as recommended by the Sigma-Aldrich (St Louis, MI, USA).

    Plasmid Preparation:

    Article Title: Antibiotic binding of STY3178, a yfdX protein from Salmonella Typhi
    Article Snippet: .. The amplified PCR product is then inserted into TA vector using T4 DNA ligase and transformed into Top10 E.coli cells (Novagen). .. The transformed bacteria with the desired gene are confirmed by screening the blue/white colonies followed by colony PCR.

    Article Title: Molecular phylogenic location of the Plagiorchis muris (Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome C oxidase subunit I
    Article Snippet: .. Amplified PCR products were extracted and purified using a gel extraction kit and a PCR purification kit (QIAGEN Co., Germany) and were cloned into a pT7Blue Perfectly Blunt cloning vector using T4 DNA ligase and transformed into E.coli Nova Blue competent cells, according to the supplier's protocol (Novagen Co. USA). .. Positive recombinant clones were picked, and grown in 2 ml of LB broth (in the presence of 50 g/ml ampicillin) overnight at 37℃.

    Article Title: Flagellar Radial Spoke Protein 2 Is a Calmodulin Binding Protein Required for Motility in Chlamydomonas reinhardtii
    Article Snippet: .. The three fragments were ligated with T4 DNA ligase and cloned into the Nco I and Sal I sites in the expression vector pET28a (Novagen). .. Based upon sequencing and Western blotting with antibodies to RSP2, the construct expressed the authentic RSP2 (see Results and Discussion).

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  • 94
    Millipore t4 dna ligase
    T4 Dna Ligase, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/Millipore
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-05
    94/100 stars
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