t4 ligase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher t4 ligase kit
    T4 Ligase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase kit/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 ligase kit - by Bioz Stars, 2020-05
    88/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Chlamydia trachomatis recombinant MOMP encapsulated in PLGA nanoparticles triggers primarily T helper 1 cellular and antibody immune responses in mice: a desirable candidate nanovaccine
    Article Snippet: .. Cloning, expression, and purification of rMOMP Polymerase chain reaction amplification of the full-length MOMP was performed following previously published methods using Phusion Taq DNA polymerase (New England Biolabs, Ipswich, MA, USA) and the following primers: forward 5′-CGAACAGATTGGAGGTAAAAAACTCTT-GAAATCGGTATTAG-3′ and reverse 5′-CACGCGGCCGCT-TAGAAGCGGAATTGTGC-3′ and ligated in pESUMO plasmid using T4 ligase kit (Life Technologies, Grand Isle, NY, USA). .. The plasmid was sequenced (Auburn University Genomics and Sequencing Laboratory, Auburn, AL, USA) and subsequently transformed into Rosetta (DE3)Escherichia coli- competent cells for protein expression.

    Amplification:

    Article Title: Chlamydia trachomatis recombinant MOMP encapsulated in PLGA nanoparticles triggers primarily T helper 1 cellular and antibody immune responses in mice: a desirable candidate nanovaccine
    Article Snippet: .. Cloning, expression, and purification of rMOMP Polymerase chain reaction amplification of the full-length MOMP was performed following previously published methods using Phusion Taq DNA polymerase (New England Biolabs, Ipswich, MA, USA) and the following primers: forward 5′-CGAACAGATTGGAGGTAAAAAACTCTT-GAAATCGGTATTAG-3′ and reverse 5′-CACGCGGCCGCT-TAGAAGCGGAATTGTGC-3′ and ligated in pESUMO plasmid using T4 ligase kit (Life Technologies, Grand Isle, NY, USA). .. The plasmid was sequenced (Auburn University Genomics and Sequencing Laboratory, Auburn, AL, USA) and subsequently transformed into Rosetta (DE3)Escherichia coli- competent cells for protein expression.

    Ligation:

    Article Title: Genome-Wide Screen in Francisella novicida for Genes Required for Pulmonary and Systemic Infection in Mice ▿
    Article Snippet: .. Ligation was performed using the T4 ligase kit (Invitrogen) on 200 ng of digested genomic DNA with a total of 74 pmol of the Y-linker, using primers TA1 and TA2 at 16°C for 12 h as described previously ( ). ..

    Purification:

    Article Title: Chlamydia trachomatis recombinant MOMP encapsulated in PLGA nanoparticles triggers primarily T helper 1 cellular and antibody immune responses in mice: a desirable candidate nanovaccine
    Article Snippet: .. Cloning, expression, and purification of rMOMP Polymerase chain reaction amplification of the full-length MOMP was performed following previously published methods using Phusion Taq DNA polymerase (New England Biolabs, Ipswich, MA, USA) and the following primers: forward 5′-CGAACAGATTGGAGGTAAAAAACTCTT-GAAATCGGTATTAG-3′ and reverse 5′-CACGCGGCCGCT-TAGAAGCGGAATTGTGC-3′ and ligated in pESUMO plasmid using T4 ligase kit (Life Technologies, Grand Isle, NY, USA). .. The plasmid was sequenced (Auburn University Genomics and Sequencing Laboratory, Auburn, AL, USA) and subsequently transformed into Rosetta (DE3)Escherichia coli- competent cells for protein expression.

    Expressing:

    Article Title: Chlamydia trachomatis recombinant MOMP encapsulated in PLGA nanoparticles triggers primarily T helper 1 cellular and antibody immune responses in mice: a desirable candidate nanovaccine
    Article Snippet: .. Cloning, expression, and purification of rMOMP Polymerase chain reaction amplification of the full-length MOMP was performed following previously published methods using Phusion Taq DNA polymerase (New England Biolabs, Ipswich, MA, USA) and the following primers: forward 5′-CGAACAGATTGGAGGTAAAAAACTCTT-GAAATCGGTATTAG-3′ and reverse 5′-CACGCGGCCGCT-TAGAAGCGGAATTGTGC-3′ and ligated in pESUMO plasmid using T4 ligase kit (Life Technologies, Grand Isle, NY, USA). .. The plasmid was sequenced (Auburn University Genomics and Sequencing Laboratory, Auburn, AL, USA) and subsequently transformed into Rosetta (DE3)Escherichia coli- competent cells for protein expression.

    Polymerase Chain Reaction:

    Article Title: Chlamydia trachomatis recombinant MOMP encapsulated in PLGA nanoparticles triggers primarily T helper 1 cellular and antibody immune responses in mice: a desirable candidate nanovaccine
    Article Snippet: .. Cloning, expression, and purification of rMOMP Polymerase chain reaction amplification of the full-length MOMP was performed following previously published methods using Phusion Taq DNA polymerase (New England Biolabs, Ipswich, MA, USA) and the following primers: forward 5′-CGAACAGATTGGAGGTAAAAAACTCTT-GAAATCGGTATTAG-3′ and reverse 5′-CACGCGGCCGCT-TAGAAGCGGAATTGTGC-3′ and ligated in pESUMO plasmid using T4 ligase kit (Life Technologies, Grand Isle, NY, USA). .. The plasmid was sequenced (Auburn University Genomics and Sequencing Laboratory, Auburn, AL, USA) and subsequently transformed into Rosetta (DE3)Escherichia coli- competent cells for protein expression.

    Plasmid Preparation:

    Article Title: Chlamydia trachomatis recombinant MOMP encapsulated in PLGA nanoparticles triggers primarily T helper 1 cellular and antibody immune responses in mice: a desirable candidate nanovaccine
    Article Snippet: .. Cloning, expression, and purification of rMOMP Polymerase chain reaction amplification of the full-length MOMP was performed following previously published methods using Phusion Taq DNA polymerase (New England Biolabs, Ipswich, MA, USA) and the following primers: forward 5′-CGAACAGATTGGAGGTAAAAAACTCTT-GAAATCGGTATTAG-3′ and reverse 5′-CACGCGGCCGCT-TAGAAGCGGAATTGTGC-3′ and ligated in pESUMO plasmid using T4 ligase kit (Life Technologies, Grand Isle, NY, USA). .. The plasmid was sequenced (Auburn University Genomics and Sequencing Laboratory, Auburn, AL, USA) and subsequently transformed into Rosetta (DE3)Escherichia coli- competent cells for protein expression.

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    Thermo Fisher t4 dna ligase kit
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