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Promega t4 ligase kit
T4 Ligase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase kit/product/Promega
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t4 ligase kit - by Bioz Stars, 2020-05
88/100 stars

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Clone Assay:

Article Title: c-MYC Generates Repair Errors via Increased Transcription of Alternative-NHEJ Factors, LIG3 and PARP1, in Tyrosine Kinase-activated Leukemias
Article Snippet: .. The cloned promoter was digested with NheI and EcoRV restriction enzymes (New England Biolabs, Ipswich, MA) and inserted into a digested pGL4.10 vector using a T4 ligase kit (Promega) according to the manufacturer’s instructions. .. The PARP1 promoter-reporter construct was obtained from Switch Gear Genomics (Carlsbad, CA).

Amplification:

Article Title: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration
Article Snippet: .. The subcloning of control mCherry construct into the AAV vector was made with the following modifications: The primers used for the PCR for DNA amplification were as follows: forward (containing EcoRI site), 5′-CCGGGAATTCACCATGGTGAGCAAGGGCGA-3′; reverse (containing XbaI site), 5′-CCGGTCTAGACTTGTACAGCTCGTCCAT-3′; which produced a DNA fragment of ∼700 bp. mCherry and kindlin-1-mCherry inserts and AAV vector were ligated using a T4 ligase kit (Promega), and the resulting ligation products were then transformed into bacteria. .. A single clone containing the correct sequence was then amplified and purified using a Megaprep Kit (Invitrogen).

Ligation:

Article Title: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration
Article Snippet: .. The subcloning of control mCherry construct into the AAV vector was made with the following modifications: The primers used for the PCR for DNA amplification were as follows: forward (containing EcoRI site), 5′-CCGGGAATTCACCATGGTGAGCAAGGGCGA-3′; reverse (containing XbaI site), 5′-CCGGTCTAGACTTGTACAGCTCGTCCAT-3′; which produced a DNA fragment of ∼700 bp. mCherry and kindlin-1-mCherry inserts and AAV vector were ligated using a T4 ligase kit (Promega), and the resulting ligation products were then transformed into bacteria. .. A single clone containing the correct sequence was then amplified and purified using a Megaprep Kit (Invitrogen).

Subcloning:

Article Title: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration
Article Snippet: .. The subcloning of control mCherry construct into the AAV vector was made with the following modifications: The primers used for the PCR for DNA amplification were as follows: forward (containing EcoRI site), 5′-CCGGGAATTCACCATGGTGAGCAAGGGCGA-3′; reverse (containing XbaI site), 5′-CCGGTCTAGACTTGTACAGCTCGTCCAT-3′; which produced a DNA fragment of ∼700 bp. mCherry and kindlin-1-mCherry inserts and AAV vector were ligated using a T4 ligase kit (Promega), and the resulting ligation products were then transformed into bacteria. .. A single clone containing the correct sequence was then amplified and purified using a Megaprep Kit (Invitrogen).

Construct:

Article Title: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration
Article Snippet: .. The subcloning of control mCherry construct into the AAV vector was made with the following modifications: The primers used for the PCR for DNA amplification were as follows: forward (containing EcoRI site), 5′-CCGGGAATTCACCATGGTGAGCAAGGGCGA-3′; reverse (containing XbaI site), 5′-CCGGTCTAGACTTGTACAGCTCGTCCAT-3′; which produced a DNA fragment of ∼700 bp. mCherry and kindlin-1-mCherry inserts and AAV vector were ligated using a T4 ligase kit (Promega), and the resulting ligation products were then transformed into bacteria. .. A single clone containing the correct sequence was then amplified and purified using a Megaprep Kit (Invitrogen).

Produced:

Article Title: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration
Article Snippet: .. The subcloning of control mCherry construct into the AAV vector was made with the following modifications: The primers used for the PCR for DNA amplification were as follows: forward (containing EcoRI site), 5′-CCGGGAATTCACCATGGTGAGCAAGGGCGA-3′; reverse (containing XbaI site), 5′-CCGGTCTAGACTTGTACAGCTCGTCCAT-3′; which produced a DNA fragment of ∼700 bp. mCherry and kindlin-1-mCherry inserts and AAV vector were ligated using a T4 ligase kit (Promega), and the resulting ligation products were then transformed into bacteria. .. A single clone containing the correct sequence was then amplified and purified using a Megaprep Kit (Invitrogen).

Polymerase Chain Reaction:

Article Title: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration
Article Snippet: .. The subcloning of control mCherry construct into the AAV vector was made with the following modifications: The primers used for the PCR for DNA amplification were as follows: forward (containing EcoRI site), 5′-CCGGGAATTCACCATGGTGAGCAAGGGCGA-3′; reverse (containing XbaI site), 5′-CCGGTCTAGACTTGTACAGCTCGTCCAT-3′; which produced a DNA fragment of ∼700 bp. mCherry and kindlin-1-mCherry inserts and AAV vector were ligated using a T4 ligase kit (Promega), and the resulting ligation products were then transformed into bacteria. .. A single clone containing the correct sequence was then amplified and purified using a Megaprep Kit (Invitrogen).

Transformation Assay:

Article Title: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration
Article Snippet: .. The subcloning of control mCherry construct into the AAV vector was made with the following modifications: The primers used for the PCR for DNA amplification were as follows: forward (containing EcoRI site), 5′-CCGGGAATTCACCATGGTGAGCAAGGGCGA-3′; reverse (containing XbaI site), 5′-CCGGTCTAGACTTGTACAGCTCGTCCAT-3′; which produced a DNA fragment of ∼700 bp. mCherry and kindlin-1-mCherry inserts and AAV vector were ligated using a T4 ligase kit (Promega), and the resulting ligation products were then transformed into bacteria. .. A single clone containing the correct sequence was then amplified and purified using a Megaprep Kit (Invitrogen).

Plasmid Preparation:

Article Title: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration
Article Snippet: .. The subcloning of control mCherry construct into the AAV vector was made with the following modifications: The primers used for the PCR for DNA amplification were as follows: forward (containing EcoRI site), 5′-CCGGGAATTCACCATGGTGAGCAAGGGCGA-3′; reverse (containing XbaI site), 5′-CCGGTCTAGACTTGTACAGCTCGTCCAT-3′; which produced a DNA fragment of ∼700 bp. mCherry and kindlin-1-mCherry inserts and AAV vector were ligated using a T4 ligase kit (Promega), and the resulting ligation products were then transformed into bacteria. .. A single clone containing the correct sequence was then amplified and purified using a Megaprep Kit (Invitrogen).

Article Title: c-MYC Generates Repair Errors via Increased Transcription of Alternative-NHEJ Factors, LIG3 and PARP1, in Tyrosine Kinase-activated Leukemias
Article Snippet: .. The cloned promoter was digested with NheI and EcoRV restriction enzymes (New England Biolabs, Ipswich, MA) and inserted into a digested pGL4.10 vector using a T4 ligase kit (Promega) according to the manufacturer’s instructions. .. The PARP1 promoter-reporter construct was obtained from Switch Gear Genomics (Carlsbad, CA).

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    Promega t4 ligase kit
    T4 Ligase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase kit/product/Promega
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 ligase kit - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

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