Structured Review

Enzymatics t4 ligase buffer
T4 Ligase Buffer, supplied by Enzymatics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase buffer/product/Enzymatics
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t4 ligase buffer - by Bioz Stars, 2020-05
90/100 stars

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Sequencing:

Article Title: Variable chromatin structure revealed by in situ spatially correlated DNA cleavage mapping
Article Snippet: .. Using a method adapted from Smith et al. , purified ssDNA fragments were ligated to sequencing adapters with six-random-nucleotide overhangs ( ) using 50 pmol of each adaptor, 1 × T4 Ligase Buffer (Enzymatics), and 450 U of T4 DNA Ligase (Enzymatics) at 15 °C for 16 h with mixing at 800 r.p.m. (Eppendorf Thermomixer) in a volume of 25 μL per 4.5 mm square plug equivalent. .. Excess adapters were removed with S-300-HR spin columns (Bio-Rad), MgCl2 was added to restore magnesium concentration to 10 mM, and the 3′ adaptor was extended using 0.02 U/μL Taq-B enzyme, 1 × PCR Buffer (both, Enzymatics) and 0.2 mM (each) dNTPs (New England Biolabs) at 65 °C for 30 min.

Incubation:

Article Title: Diverse motif ensembles specify non-redundant DNA binding activities of AP-1 family members in macrophages
Article Snippet: .. Library prep protocol dsDNA end repair: We mixed 40 μL of DNA from ChIP or RNA protocols with 2.9 μL of H2 O, 0.5 μL 1% Tween-20, 5 μL 10× T4 ligase buffer (Enzymatics cat# L6030-HC-L), 1 μL dNTP mix (10 mM Affymetrix 77119), 0.3 μL T4 DNA pol (Enzymatics P7080L), 0.3 μL T4 PNK (Enzymatics Y9040L), 0.06 μL Klenow (Enzymatics P7060L) and incubated for 30 min at 20 °C. .. 1 μL of Seradyn 3 EDAC SpeedBeads (Thermo 6515-2105-050250) in 93 μL 20% PEG8000/2.5 M NaCl (13% final) was added and incubated for 10 min. Bead clean-up: Beads were collected on a magnet and washed 2× with 80% ethanol.

Article Title: Diverse motif ensembles specify non-redundant DNA binding activities of AP-1 family members in macrophages
Article Snippet: .. dsDNA end repair: We mixed 40 μL of DNA from ChIP or RNA protocols with 2.9 μL of H2 O, 0.5 μL 1% Tween-20, 5 μL 10× T4 ligase buffer (Enzymatics cat# L6030-HC-L), 1 μL dNTP mix (10 mM Affymetrix 77119), 0.3 μL T4 DNA pol (Enzymatics P7080L), 0.3 μL T4 PNK (Enzymatics Y9040L), 0.06 μL Klenow (Enzymatics P7060L) and incubated for 30 min at 20 °C. .. 1 μL of Seradyn 3 EDAC SpeedBeads (Thermo 6515-2105-050250) in 93 μL 20% PEG8000/2.5 M NaCl (13% final) was added and incubated for 10 min. Bead clean-up: Beads were collected on a magnet and washed 2× with 80% ethanol.

Chromatin Immunoprecipitation:

Article Title: Diverse motif ensembles specify non-redundant DNA binding activities of AP-1 family members in macrophages
Article Snippet: .. Library prep protocol dsDNA end repair: We mixed 40 μL of DNA from ChIP or RNA protocols with 2.9 μL of H2 O, 0.5 μL 1% Tween-20, 5 μL 10× T4 ligase buffer (Enzymatics cat# L6030-HC-L), 1 μL dNTP mix (10 mM Affymetrix 77119), 0.3 μL T4 DNA pol (Enzymatics P7080L), 0.3 μL T4 PNK (Enzymatics Y9040L), 0.06 μL Klenow (Enzymatics P7060L) and incubated for 30 min at 20 °C. .. 1 μL of Seradyn 3 EDAC SpeedBeads (Thermo 6515-2105-050250) in 93 μL 20% PEG8000/2.5 M NaCl (13% final) was added and incubated for 10 min. Bead clean-up: Beads were collected on a magnet and washed 2× with 80% ethanol.

Article Title: Diverse motif ensembles specify non-redundant DNA binding activities of AP-1 family members in macrophages
Article Snippet: .. dsDNA end repair: We mixed 40 μL of DNA from ChIP or RNA protocols with 2.9 μL of H2 O, 0.5 μL 1% Tween-20, 5 μL 10× T4 ligase buffer (Enzymatics cat# L6030-HC-L), 1 μL dNTP mix (10 mM Affymetrix 77119), 0.3 μL T4 DNA pol (Enzymatics P7080L), 0.3 μL T4 PNK (Enzymatics Y9040L), 0.06 μL Klenow (Enzymatics P7060L) and incubated for 30 min at 20 °C. .. 1 μL of Seradyn 3 EDAC SpeedBeads (Thermo 6515-2105-050250) in 93 μL 20% PEG8000/2.5 M NaCl (13% final) was added and incubated for 10 min. Bead clean-up: Beads were collected on a magnet and washed 2× with 80% ethanol.

Purification:

Article Title: Variable chromatin structure revealed by in situ spatially correlated DNA cleavage mapping
Article Snippet: .. Using a method adapted from Smith et al. , purified ssDNA fragments were ligated to sequencing adapters with six-random-nucleotide overhangs ( ) using 50 pmol of each adaptor, 1 × T4 Ligase Buffer (Enzymatics), and 450 U of T4 DNA Ligase (Enzymatics) at 15 °C for 16 h with mixing at 800 r.p.m. (Eppendorf Thermomixer) in a volume of 25 μL per 4.5 mm square plug equivalent. .. Excess adapters were removed with S-300-HR spin columns (Bio-Rad), MgCl2 was added to restore magnesium concentration to 10 mM, and the 3′ adaptor was extended using 0.02 U/μL Taq-B enzyme, 1 × PCR Buffer (both, Enzymatics) and 0.2 mM (each) dNTPs (New England Biolabs) at 65 °C for 30 min.

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    Enzymatics 1x rapid t4 ligase buffer
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