t4 dna ligase hc  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    T4 DNA Ligase
    Description:
    Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA The enzyme repairs single strand nicks in duplex DNA RNA or DNA RNA hybrids It also joins DNA fragments with either cohesive or blunt termini but has no activity on single stranded nucleic acids T4 DNA Ligase requires ATP as a cofactor Highlights• Active in Themo Scientific restriction enzyme PCR and RT buffers when supplemented with ATP • Fast sticky end ligation is completed in 10 minutes at room temperature• Supplied with PEG solution for efficient blunt end ligationApplications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Joining of double stranded oligonucleotide linkers or adaptors to DNA• Site directed mutagenesis• Amplified fragment length polymorphism AFLP • Ligase mediated RNA detection see Reference 3 • Nick repair in duplex DNA RNA or DNA RNA hybrids• Self circularization of linear DNA Includes• T4 DNA Ligase• 10X T4 DNA Ligase Buffer• 50 PEG SolutionNotes• Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels To avoid this incubate samples with 6X DNA Loading Dye SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis • The volume of the ligation reaction mixture should not exceed 10 of the competent cell volume in the transformation process • Prior to electro transformation remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction The extracted DNA can be further precipitated with ethanol
    Catalog Number:
    el0013
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher t4 dna ligase hc
    Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA The enzyme repairs single strand nicks in duplex DNA RNA or DNA RNA hybrids It also joins DNA fragments with either cohesive or blunt termini but has no activity on single stranded nucleic acids T4 DNA Ligase requires ATP as a cofactor Highlights• Active in Themo Scientific restriction enzyme PCR and RT buffers when supplemented with ATP • Fast sticky end ligation is completed in 10 minutes at room temperature• Supplied with PEG solution for efficient blunt end ligationApplications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Joining of double stranded oligonucleotide linkers or adaptors to DNA• Site directed mutagenesis• Amplified fragment length polymorphism AFLP • Ligase mediated RNA detection see Reference 3 • Nick repair in duplex DNA RNA or DNA RNA hybrids• Self circularization of linear DNA Includes• T4 DNA Ligase• 10X T4 DNA Ligase Buffer• 50 PEG SolutionNotes• Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels To avoid this incubate samples with 6X DNA Loading Dye SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis • The volume of the ligation reaction mixture should not exceed 10 of the competent cell volume in the transformation process • Prior to electro transformation remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction The extracted DNA can be further precipitated with ethanol
    https://www.bioz.com/result/t4 dna ligase hc/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase hc - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Reverse transcriptase and endonuclease activities encoded by Penelope-like retroelements
    Article Snippet: .. After addition of 0.5 Weiss unit of T4 DNA ligase (Fermentas, Hanover, MD), reactions were incubated at 16°C and analyzed by denaturing gel electrophoresis as above. ..

    Synthesized:

    Article Title: Practical Synthesis of Cap‐4 RNA
    Article Snippet: .. The 38‐nt T. cruzi cap‐4 RNA was prepared by splinted enzymatic ligation of an 11‐nt cap‐4 RNA and a chemically synthesized 5′‐phosphorylated 27‐nt RNA by using T4 DNA ligase (Thermo Fisher) in analogy to ref. . ..

    Ligation:

    Article Title: Identification of recognition residues for ligation-based detection and quantitation of pseudouridine and N6-methyladenosine
    Article Snippet: .. The optimized ligation reactions were carried out with 0.15 µM 30-mer RNA with or without Ψ or m6 A modifications, 0.5 µM floater and 0.38 µM of 5′-32 P-labeled anchor in 66 mM Tris–HCl, pH 7.6, 0.5 mM ZnCl2 , 10 mM DTT, 66 µM ATP, 15% DMSO and 0.25 U/µl T4 DNA ligase (USB Inc.). .. All components were mixed and incubated at 16°C for 16 h, and the ligation products separated on denaturing polyacrylamide gels containing 7 M urea.

    Article Title: Practical Synthesis of Cap‐4 RNA
    Article Snippet: .. The 38‐nt T. cruzi cap‐4 RNA was prepared by splinted enzymatic ligation of an 11‐nt cap‐4 RNA and a chemically synthesized 5′‐phosphorylated 27‐nt RNA by using T4 DNA ligase (Thermo Fisher) in analogy to ref. . ..

    Article Title: Profiling non-lysyl tRNAs in HIV-1
    Article Snippet: .. The ligation reaction was carried out overnight (∼16 h) at 16°C with 0.13 μg/μL total RNA in 1X T4 DNA ligase buffer, 0.5 U/μL T4 DNA ligase (USB Corporation, 70042X), 15% DMSO, and 4.5 μM labeling oligonucleotide. .. Hybridization was performed at 60°C overnight (∼16 h) with 1–2 μg each of Cy3- or Cy5-labeled total RNA as previously described ( ).

    Labeling:

    Article Title: Profiling non-lysyl tRNAs in HIV-1
    Article Snippet: .. The ligation reaction was carried out overnight (∼16 h) at 16°C with 0.13 μg/μL total RNA in 1X T4 DNA ligase buffer, 0.5 U/μL T4 DNA ligase (USB Corporation, 70042X), 15% DMSO, and 4.5 μM labeling oligonucleotide. .. Hybridization was performed at 60°C overnight (∼16 h) with 1–2 μg each of Cy3- or Cy5-labeled total RNA as previously described ( ).

    Purification:

    Article Title: FSH1 regulates the phenotype and pathogenicity of the pathogenic dermatophyte Microsporum canis
    Article Snippet: .. First, the purified product of PCR for the FSH1 gene was ligated into pUC-PUT following DNA digestion with Xho I and Hin dIII, and ligated by T4 DNA ligase (Invitrogen; Thermo Fisher Scientific, Inc.). ..

    Incubation:

    Article Title: Autophosphorylation-dependent remodeling of the DNA-dependent protein kinase catalytic subunit regulates ligation of DNA ends
    Article Snippet: .. Reactions were started by the addition of 0.5 U of T4 DNA ligase (Invitrogen, Carlsbad, CA) and incubated at 37°C. ..

    Article Title: Reverse transcriptase and endonuclease activities encoded by Penelope-like retroelements
    Article Snippet: .. After addition of 0.5 Weiss unit of T4 DNA ligase (Fermentas, Hanover, MD), reactions were incubated at 16°C and analyzed by denaturing gel electrophoresis as above. ..

    Polymerase Chain Reaction:

    Article Title: FSH1 regulates the phenotype and pathogenicity of the pathogenic dermatophyte Microsporum canis
    Article Snippet: .. First, the purified product of PCR for the FSH1 gene was ligated into pUC-PUT following DNA digestion with Xho I and Hin dIII, and ligated by T4 DNA ligase (Invitrogen; Thermo Fisher Scientific, Inc.). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher t4 dna ligase hc
    T4 Dna Ligase Hc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase hc/product/Thermo Fisher
    Average 99 stars, based on 557 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase hc - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results