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SPL Life Sciences t25 flasks
T25 Flasks, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flasks/product/SPL Life Sciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t25 flasks - by Bioz Stars, 2020-04
94/100 stars

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Cell Isolation:

Article Title: IL-27 enhances IL-15/IL-18-mediated activation of human natural killer cells
Article Snippet: Paragraph title: Human natural killer (NK) cell isolation and ex vivo expansion ... After 7 days, the cells were transferred into T25 flasks, T75 flasks, and T175 flasks (SPL Life Sciences, KOR) with additional media containing cytokines for 21 days.

Inverted Microscopy:

Article Title: Individual expression features of GPX2, NQO1 andSQSTM1 transcript variants induced by hydrogen peroxide treatment in HeLa cells
Article Snippet: The cells were grown in T25 flasks, 24- and 96-well plates (SPL Lifesciences, South Korea) in GlutaMax DMEM medium (Thermo Fisher Scientific, USA) supplemented with 10% of fetal bovine serum (GE Healthcare, UK) and 0.05 μg/ml of gentamicin (Biokhimik JSC, Russia). .. Cell growth was controlled using the Premiere MIS-9000 inverted microscope (C & A, China).

Isolation:

Article Title: IL-27 enhances IL-15/IL-18-mediated activation of human natural killer cells
Article Snippet: PBMCs were isolated from whole blood by a density gradient with Ficoll-Paque Plus (GE Health Care, Piscataway, NJ) followed by purification using an NK cell isolation kit (Miltenyi Biotec, Germany). .. After 7 days, the cells were transferred into T25 flasks, T75 flasks, and T175 flasks (SPL Life Sciences, KOR) with additional media containing cytokines for 21 days.

Cell Culture:

Article Title: Individual expression features of GPX2, NQO1 andSQSTM1 transcript variants induced by hydrogen peroxide treatment in HeLa cells
Article Snippet: Paragraph title: Cell culture, hydrogen peroxide treatment and viability assay ... The cells were grown in T25 flasks, 24- and 96-well plates (SPL Lifesciences, South Korea) in GlutaMax DMEM medium (Thermo Fisher Scientific, USA) supplemented with 10% of fetal bovine serum (GE Healthcare, UK) and 0.05 μg/ml of gentamicin (Biokhimik JSC, Russia).

Article Title: Polyamidoamine Nanoparticles for the Oral Administration of Antimalarial Drugs
Article Snippet: Paragraph title: 2.3. Plasmodium falciparum Cell Culture and Parasite Growth Inhibition, Hemolysis and Unspecific Cytotoxicity Assays ... Parasites were grown at 37 °C in T25 flasks (SPL Life Sciences, Gyeonggi-do, Korea) containing human RBCs of blood group type B in a Roswell Park Memorial Institute (RPMI) medium supplemented with 5 g/L Albumax II and 2 mM glutamine (complete RPMI), following previously established conditions [ ].

Article Title: Low toxic maghemite nanoparticles for theranostic applications
Article Snippet: .. The cells were sub-cultured in T25 flasks and further cultured for the analysis in 96-well plates (SPL Life Sciences, Pocheon-si, South Korea) in the GlutaMax DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% of fetal bovine serum (GE Healthcare UK Ltd, Little Chalfont, UK) and 0.05 μg/mL of gentamicin (Biokhimik JSC, Saransk, Russia). .. The cells were kept at 37°C and 5% CO2 , with passive humidification in the Sanyo MCO-18AC incubator (Panasonic, Moriguchi, Japan).

Article Title: Voltage-Gated K+ Channel, Kv3.3 Is Involved in Hemin-Induced K562 Differentiation
Article Snippet: Paragraph title: 2.1. Cell culture and hemin-induced cell differentiation ... T25 flasks (SPL Life Sciences, Gyeonggi-do, Korea) were used for culturing the cells.

Purification:

Article Title: IL-27 enhances IL-15/IL-18-mediated activation of human natural killer cells
Article Snippet: PBMCs were isolated from whole blood by a density gradient with Ficoll-Paque Plus (GE Health Care, Piscataway, NJ) followed by purification using an NK cell isolation kit (Miltenyi Biotec, Germany). .. After 7 days, the cells were transferred into T25 flasks, T75 flasks, and T175 flasks (SPL Life Sciences, KOR) with additional media containing cytokines for 21 days.

Concentration Assay:

Article Title: Low toxic maghemite nanoparticles for theranostic applications
Article Snippet: The cells were sub-cultured in T25 flasks and further cultured for the analysis in 96-well plates (SPL Life Sciences, Pocheon-si, South Korea) in the GlutaMax DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% of fetal bovine serum (GE Healthcare UK Ltd, Little Chalfont, UK) and 0.05 μg/mL of gentamicin (Biokhimik JSC, Saransk, Russia). .. For the cell viability assay, the nanoparticle formulation was re-suspended in physiological saline, so as to reach the maximum possible concentration without forming a precipitate.

Incubation:

Article Title: Polyamidoamine Nanoparticles for the Oral Administration of Antimalarial Drugs
Article Snippet: Parasites were grown at 37 °C in T25 flasks (SPL Life Sciences, Gyeonggi-do, Korea) containing human RBCs of blood group type B in a Roswell Park Memorial Institute (RPMI) medium supplemented with 5 g/L Albumax II and 2 mM glutamine (complete RPMI), following previously established conditions [ ]. .. Two hundred microliters of these Plasmodium cultures were plated in 96-well plates and incubated for 48 h at 37 °C in the presence of free drugs and PAA-drug conjugates dissolved in RPMI.

Article Title: Voltage-Gated K+ Channel, Kv3.3 Is Involved in Hemin-Induced K562 Differentiation
Article Snippet: Cell culture and hemin-induced cell differentiation K562 cells obtained from Korean Cell Line Bank were cultured in RPMI1640 medium (Welgene, Daegu, Korea) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution (Sigma, St. Louis, MO) at 37°C incubation with 5% CO2 . .. T25 flasks (SPL Life Sciences, Gyeonggi-do, Korea) were used for culturing the cells.

Inhibition:

Article Title: Polyamidoamine Nanoparticles for the Oral Administration of Antimalarial Drugs
Article Snippet: Paragraph title: 2.3. Plasmodium falciparum Cell Culture and Parasite Growth Inhibition, Hemolysis and Unspecific Cytotoxicity Assays ... Parasites were grown at 37 °C in T25 flasks (SPL Life Sciences, Gyeonggi-do, Korea) containing human RBCs of blood group type B in a Roswell Park Memorial Institute (RPMI) medium supplemented with 5 g/L Albumax II and 2 mM glutamine (complete RPMI), following previously established conditions [ ].

Mass Spectrometry:

Article Title: Low Intensity and Frequency Pulsed Electromagnetic Fields Selectively Impair Breast Cancer Cell Viability
Article Snippet: PEMFs treatment MCF7 and MCF10 cells were seeded in T25 flasks (SPL Life Sciences, Korea) at concentrations of 6.5×105 cells/ml and 6.7×105 cells/ml, respectively. .. An asymmetric pulsed magnetic field of 6 ms interval at a repetition rate of 20 and 50 Hz were applied at flux densities of 2.0, 3.0 and 5.0 mT (peak-to-peak) for 1 hour/day for three days.

Ex Vivo:

Article Title: IL-27 enhances IL-15/IL-18-mediated activation of human natural killer cells
Article Snippet: Paragraph title: Human natural killer (NK) cell isolation and ex vivo expansion ... After 7 days, the cells were transferred into T25 flasks, T75 flasks, and T175 flasks (SPL Life Sciences, KOR) with additional media containing cytokines for 21 days.

Cell Differentiation:

Article Title: Voltage-Gated K+ Channel, Kv3.3 Is Involved in Hemin-Induced K562 Differentiation
Article Snippet: Paragraph title: 2.1. Cell culture and hemin-induced cell differentiation ... T25 flasks (SPL Life Sciences, Gyeonggi-do, Korea) were used for culturing the cells.

Viability Assay:

Article Title: Individual expression features of GPX2, NQO1 andSQSTM1 transcript variants induced by hydrogen peroxide treatment in HeLa cells
Article Snippet: Paragraph title: Cell culture, hydrogen peroxide treatment and viability assay ... The cells were grown in T25 flasks, 24- and 96-well plates (SPL Lifesciences, South Korea) in GlutaMax DMEM medium (Thermo Fisher Scientific, USA) supplemented with 10% of fetal bovine serum (GE Healthcare, UK) and 0.05 μg/ml of gentamicin (Biokhimik JSC, Russia).

Article Title: Low toxic maghemite nanoparticles for theranostic applications
Article Snippet: The cells were sub-cultured in T25 flasks and further cultured for the analysis in 96-well plates (SPL Life Sciences, Pocheon-si, South Korea) in the GlutaMax DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% of fetal bovine serum (GE Healthcare UK Ltd, Little Chalfont, UK) and 0.05 μg/mL of gentamicin (Biokhimik JSC, Saransk, Russia). .. For the cell viability assay, the nanoparticle formulation was re-suspended in physiological saline, so as to reach the maximum possible concentration without forming a precipitate.

In Vitro:

Article Title: Polyamidoamine Nanoparticles for the Oral Administration of Antimalarial Drugs
Article Snippet: P. falciparum 3D7 in vitro cultures were prepared as described elsewhere [ , ]. .. Parasites were grown at 37 °C in T25 flasks (SPL Life Sciences, Gyeonggi-do, Korea) containing human RBCs of blood group type B in a Roswell Park Memorial Institute (RPMI) medium supplemented with 5 g/L Albumax II and 2 mM glutamine (complete RPMI), following previously established conditions [ ].

Article Title: Low toxic maghemite nanoparticles for theranostic applications
Article Snippet: Toxicity and cell viability testing For in vitro cytotoxicity testing, we used HeLa cell line as the model for the cytotoxicity test. .. The cells were sub-cultured in T25 flasks and further cultured for the analysis in 96-well plates (SPL Life Sciences, Pocheon-si, South Korea) in the GlutaMax DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% of fetal bovine serum (GE Healthcare UK Ltd, Little Chalfont, UK) and 0.05 μg/mL of gentamicin (Biokhimik JSC, Saransk, Russia).

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  • 94
    SPL Life Sciences t25 flask
    T25 Flask, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/SPL Life Sciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    94
    SPL Life Sciences t25 flasks
    T25 Flasks, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/SPL Life Sciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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