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PAA Laboratories t25 flasks
T25 Flasks, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flasks/product/PAA Laboratories
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t25 flasks - by Bioz Stars, 2020-04
92/100 stars

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Flow Cytometry:

Article Title: Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons
Article Snippet: .. Flow cytometric measurements of reactive oxygen species (ROS) Cells were seeded in T25 flasks (PAA Laboratories GmbH, Cölbe, Germany), at 0.6 × 106 for A549 cells and at 1 × 106 for 16HBE14o- cells. .. Subconfluent cultures were exposed to CBNP at a concentration of 10 μg/ml or 50 μg/ml for 24 h. The culture medium was subsequently removed and the cell monolayers were washed twice with 5 ml pre-warmed phosphate buffered saline (PBS).

Isolation:

Article Title: Cellular trafficking determines the exon skipping activity of Pip6a-PMO in mdx skeletal and cardiac muscle cells
Article Snippet: .. Briefly, freshly isolated cells were seeded in T25 flasks to allow selective adhesion of cardiac fibroblasts ( ) in plating medium: 250 ml DMEM (PAA laboratories), 250 ml M199 (Sigma-Aldrich), 5 ml glutamax (100X, Gibco®, Life Technologies), 5 ml PS, 50 ml horse serum (HS, Sigma-Aldrich) and 25 ml FBS (PAA laboratories). .. Cardiomyocytes remaining in the supernatant were seeded in well culture plates coated with 0.01% gelatin (Sigma-Aldrich).

Cell Culture:

Article Title: Local viscoelasticity of living cells measured by rotational magnetic spectroscopy
Article Snippet: .. Cell culture NIH/3T3 fibroblast (ATCC-CRL-1658) and HeLa (ATCC-CCL-2) cells were grown in T25-flasks as a monolayer in DMEM with high glucose (4.5 g l−1 ) and stable glutamine (PAA Laboratories GmbH, Austria). .. The medium was supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (PAA Laboratories GmbH), referred to as cell culture medium.

Article Title: Cellular trafficking determines the exon skipping activity of Pip6a-PMO in mdx skeletal and cardiac muscle cells
Article Snippet: Paragraph title: Cell culture ... Briefly, freshly isolated cells were seeded in T25 flasks to allow selective adhesion of cardiac fibroblasts ( ) in plating medium: 250 ml DMEM (PAA laboratories), 250 ml M199 (Sigma-Aldrich), 5 ml glutamax (100X, Gibco®, Life Technologies), 5 ml PS, 50 ml horse serum (HS, Sigma-Aldrich) and 25 ml FBS (PAA laboratories).

Mouse Assay:

Article Title: Cellular trafficking determines the exon skipping activity of Pip6a-PMO in mdx skeletal and cardiac muscle cells
Article Snippet: Primary cardiomyocytes were isolated from the ventricles of 1- to 2-day-old newborn wild-type C57BL/6 J mice (Charles River) or mdx mice (provided by M. Wood) by enzymatic digestion with type-4 collagenase (Serlabo Technologies) and pancreatin (Sigma-Aldrich), as described previously ( ). .. Briefly, freshly isolated cells were seeded in T25 flasks to allow selective adhesion of cardiac fibroblasts ( ) in plating medium: 250 ml DMEM (PAA laboratories), 250 ml M199 (Sigma-Aldrich), 5 ml glutamax (100X, Gibco®, Life Technologies), 5 ml PS, 50 ml horse serum (HS, Sigma-Aldrich) and 25 ml FBS (PAA laboratories).

Concentration Assay:

Article Title: Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons
Article Snippet: Flow cytometric measurements of reactive oxygen species (ROS) Cells were seeded in T25 flasks (PAA Laboratories GmbH, Cölbe, Germany), at 0.6 × 106 for A549 cells and at 1 × 106 for 16HBE14o- cells. .. Subconfluent cultures were exposed to CBNP at a concentration of 10 μg/ml or 50 μg/ml for 24 h. The culture medium was subsequently removed and the cell monolayers were washed twice with 5 ml pre-warmed phosphate buffered saline (PBS).

Incubation:

Article Title: Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons
Article Snippet: Flow cytometric measurements of reactive oxygen species (ROS) Cells were seeded in T25 flasks (PAA Laboratories GmbH, Cölbe, Germany), at 0.6 × 106 for A549 cells and at 1 × 106 for 16HBE14o- cells. .. The fluorescent dye dichloro-dihydro-fluorescein diacetate (DCFH-DA) was applied to the cells in 5 ml fresh medium, with the resulting mixture incubated for 30 min at 37 °C.

Quantitative RT-PCR:

Article Title: Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons
Article Snippet: .. Quantitative RT-PCR A549 cells were seeded in T25 flasks at 0.6 × 106 . .. Calu-3 cells and 16HBE14o- cells were seeded in 12-well plates (Costar, Corning, NY, USA) at 5 × 105 cells and 1.6 × 105 cells, respectively.

Modification:

Article Title: Cellular trafficking determines the exon skipping activity of Pip6a-PMO in mdx skeletal and cardiac muscle cells
Article Snippet: Cell culture Murine H2k mdx myoblasts were cultured in gelatin (0.01%)-coated flasks at 33°C, under 10% CO2 , in Dulbecco’s modified Eagles medium (DMEM PAA laboratories) supplemented with 20% heat-inactivated fetal bovine serum (FBS Gold, PAA laboratories), 2% chicken embryo extract (Seralab), 1% penicillin-streptomycin-neomycin antibiotic mixture (PSN, Gibco) and 3 pg/µl γ-interferon (PeproTech).Murine C2C12 myoblasts (ATCC: CRL–1772™) were grown in DMEM supplemented with 10% heat inactivated FBS (PAA laboratories) and 1% PSN. .. Briefly, freshly isolated cells were seeded in T25 flasks to allow selective adhesion of cardiac fibroblasts ( ) in plating medium: 250 ml DMEM (PAA laboratories), 250 ml M199 (Sigma-Aldrich), 5 ml glutamax (100X, Gibco®, Life Technologies), 5 ml PS, 50 ml horse serum (HS, Sigma-Aldrich) and 25 ml FBS (PAA laboratories).

Western Blot:

Article Title: Cellular trafficking determines the exon skipping activity of Pip6a-PMO in mdx skeletal and cardiac muscle cells
Article Snippet: The differentiation of H2k mdx and C2C12 cells were monitored by measuring the Troponin T level by ‘western blot’ (data not shown). .. Briefly, freshly isolated cells were seeded in T25 flasks to allow selective adhesion of cardiac fibroblasts ( ) in plating medium: 250 ml DMEM (PAA laboratories), 250 ml M199 (Sigma-Aldrich), 5 ml glutamax (100X, Gibco®, Life Technologies), 5 ml PS, 50 ml horse serum (HS, Sigma-Aldrich) and 25 ml FBS (PAA laboratories).

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    PAA Laboratories t25 cell culture flasks
    T25 Cell Culture Flasks, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 cell culture flasks/product/PAA Laboratories
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    t25 cell culture flasks - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    86
    PAA Laboratories t25 cell culture flask
    T25 Cell Culture Flask, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 cell culture flask/product/PAA Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t25 cell culture flask - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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