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Fisher Scientific t25 flasks
T25 Flasks, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flasks/product/Fisher Scientific
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
t25 flasks - by Bioz Stars, 2020-04
93/100 stars

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Clone Assay:

Article Title: Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells
Article Snippet: Cultures were monitored for the emergence of hiPSC clones over the next 2 wk. iPSC colonies were identified morphologically and dissected using a pipet tip. .. Every third day, cultures were passaged with an in-house formulation of hypertonic citrate buffer of 1:2 to 1:3 while expanding into T25 flasks (Fischer Scientific).

In Vitro:

Article Title: Low Dose Inflammatory Potential of Silica Particles in Human-Derived THP-1 Macrophage Cell Culture Studies – Mechanism and Effects of Particle Size and Iron
Article Snippet: THP-1 cells (Human acute monocytic leukemia cell line) purchased from American Type Culture Collection, TIB-202, were cultured in T25 flasks (Fisher Scientific) with RPMI 1640 culture medium supplemented with 10% Fetal Bovine Serum (FBS) (Omega Scientific), β-mercaptoethanol, 100 μg/ml penicillin, and 100 μg/ml streptomycin at 37 °C in a humidified 5% CO2 atmosphere. .. In recent years, these cells have been widely established as an in vitro model for native monocyte-derived macrophages in studies of inflammatory disease by particle inhalation [ , ].

Article Title: Human Neural Stem Cell Transplantation Provides Long-Term Restoration of Neuronal Plasticity in the Irradiated Hippocampus
Article Snippet: The hNSCs were maintained as a monolayer in T25 flasks (Corning, Fisher Scientific, Pittsburgh, PA, USA) in neural expansion media (EMD-Millipore), expressed stem cells markers (Sox2, nestin), and displayed multilineage potential, as described previously ( ). .. For transplantation and identification of engrafted cells in the host brain, hNSCs (passages 5–9) were labeled in vitro with BrdU (5-bromo-2′-deoxyuridine, 4 μm, labeling index 90%; Sigma-Aldrich, St. Louis, MO, USA) as previously described ( ).

Isolation:

Article Title: Differential Processing of Amyloid-? Precursor Protein Directs Human Embryonic Stem Cell Proliferation and Differentiation into Neuronal Precursor Cells *
Article Snippet: .. The NPCs in the rosettes were selectively isolated through differential enzymatic treatment using dispase (0.5 mg/ml in DMEM-F-12) and incubated for 2 h in neural induction medium to allow the non-neural cells to differentially attach to the T25 flasks. ..

Article Title: The pregnancy hormones human chorionic gonadotropin and progesterone induce human embryonic stem cell proliferation and differentiation into neuroectodermal rosettes
Article Snippet: .. The NPCs in the rosettes were selectively isolated through differential enzymatic treatment by using Dispase (0.5 mg/ml in DMEM-F12) and incubated for 2 hours in neural induction medium to allow the nonneural cells to attach differentially attach to the T25 flasks. ..

Cell Culture:

Article Title: Low Dose Inflammatory Potential of Silica Particles in Human-Derived THP-1 Macrophage Cell Culture Studies – Mechanism and Effects of Particle Size and Iron
Article Snippet: .. THP-1 cells (Human acute monocytic leukemia cell line) purchased from American Type Culture Collection, TIB-202, were cultured in T25 flasks (Fisher Scientific) with RPMI 1640 culture medium supplemented with 10% Fetal Bovine Serum (FBS) (Omega Scientific), β-mercaptoethanol, 100 μg/ml penicillin, and 100 μg/ml streptomycin at 37 °C in a humidified 5% CO2 atmosphere. .. In recent years, these cells have been widely established as an in vitro model for native monocyte-derived macrophages in studies of inflammatory disease by particle inhalation [ , ].

Labeling:

Article Title: Human Neural Stem Cell Transplantation Provides Long-Term Restoration of Neuronal Plasticity in the Irradiated Hippocampus
Article Snippet: The hNSCs were maintained as a monolayer in T25 flasks (Corning, Fisher Scientific, Pittsburgh, PA, USA) in neural expansion media (EMD-Millipore), expressed stem cells markers (Sox2, nestin), and displayed multilineage potential, as described previously ( ). .. For transplantation and identification of engrafted cells in the host brain, hNSCs (passages 5–9) were labeled in vitro with BrdU (5-bromo-2′-deoxyuridine, 4 μm, labeling index 90%; Sigma-Aldrich, St. Louis, MO, USA) as previously described ( ).

Produced:

Article Title: Glia Contribute to the Purinergic Modulation of Inspiratory Rhythm-Generating Networks
Article Snippet: The supernatant was replaced with BME-glucose solution (2 ml) that was produced by adding to 1× basal medium Eagle solution (BME; Invitrogen): 3.3 m m glucose (final concentration of 8.8 m), 2 m m l -GLN (Sigma-Aldrich), penicillin/streptomycin (10,000 U of penicillin/10,000 μg of streptomycin per ml; Invitrogen), and 10% rat serum (RS). .. The tissue was centrifuged (1500 × g , 3 min), the supernatant replaced with BME-glucose (1 ml), and tissue homogenized through trituration and centrifuged with fresh BME-glucose at 1500 × g for 6 min and 3 min. Tissue was then resuspended in BME-glucose and cells were plated on T25 flasks (Flask TC Vent 25 cm, Fisher Scientific).

Concentration Assay:

Article Title: Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells
Article Snippet: Individual colonies were scraped into culture medium, and the medium transferred to tissue culture–treated 48-well plates (Fischer Scientific) coated with rhVTN (AF-140-09; PeproTech) at a concentration of 0.5 µg/cm2 at room temperature for 1 h. Cultures were fed daily. .. Every third day, cultures were passaged with an in-house formulation of hypertonic citrate buffer of 1:2 to 1:3 while expanding into T25 flasks (Fischer Scientific).

Article Title: Glia Contribute to the Purinergic Modulation of Inspiratory Rhythm-Generating Networks
Article Snippet: The supernatant was replaced with BME-glucose solution (2 ml) that was produced by adding to 1× basal medium Eagle solution (BME; Invitrogen): 3.3 m m glucose (final concentration of 8.8 m), 2 m m l -GLN (Sigma-Aldrich), penicillin/streptomycin (10,000 U of penicillin/10,000 μg of streptomycin per ml; Invitrogen), and 10% rat serum (RS). .. The tissue was centrifuged (1500 × g , 3 min), the supernatant replaced with BME-glucose (1 ml), and tissue homogenized through trituration and centrifuged with fresh BME-glucose at 1500 × g for 6 min and 3 min. Tissue was then resuspended in BME-glucose and cells were plated on T25 flasks (Flask TC Vent 25 cm, Fisher Scientific).

Incubation:

Article Title: Differential Processing of Amyloid-? Precursor Protein Directs Human Embryonic Stem Cell Proliferation and Differentiation into Neuronal Precursor Cells *
Article Snippet: .. The NPCs in the rosettes were selectively isolated through differential enzymatic treatment using dispase (0.5 mg/ml in DMEM-F-12) and incubated for 2 h in neural induction medium to allow the non-neural cells to differentially attach to the T25 flasks. ..

Article Title: Glia Contribute to the Purinergic Modulation of Inspiratory Rhythm-Generating Networks
Article Snippet: Ventral tissue was then incubated in digestion buffer (0.25% trypsin-EDTA in PBS; Invitrogen) for 5 min. .. The tissue was centrifuged (1500 × g , 3 min), the supernatant replaced with BME-glucose (1 ml), and tissue homogenized through trituration and centrifuged with fresh BME-glucose at 1500 × g for 6 min and 3 min. Tissue was then resuspended in BME-glucose and cells were plated on T25 flasks (Flask TC Vent 25 cm, Fisher Scientific).

Article Title: The pregnancy hormones human chorionic gonadotropin and progesterone induce human embryonic stem cell proliferation and differentiation into neuroectodermal rosettes
Article Snippet: .. T25 flasks (Fisher Scientific) were incubated with 5 ml of a 2% poly (2-hydroxyethyl methacrylate) (Poly-HEMA; Sigma-Aldrich Co.) solution for 5 minutes. ..

Article Title: The pregnancy hormones human chorionic gonadotropin and progesterone induce human embryonic stem cell proliferation and differentiation into neuroectodermal rosettes
Article Snippet: .. The NPCs in the rosettes were selectively isolated through differential enzymatic treatment by using Dispase (0.5 mg/ml in DMEM-F12) and incubated for 2 hours in neural induction medium to allow the nonneural cells to attach differentially attach to the T25 flasks. ..

Article Title: Differential Processing of Amyloid-? Precursor Protein Directs Human Embryonic Stem Cell Proliferation and Differentiation into Neuronal Precursor Cells *
Article Snippet: .. T25 flasks (Fisher Scientific) were incubated with 5 ml of a 2% poly(2-hydroxyethyl methacrylate) (Sigma-Aldrich) solution for 5 min. ..

other:

Article Title: Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells
Article Snippet: Two weeks posttransduction, colonies were identified morphologically, transferred to VTN-coated 48-well plates, and culture expanded into T25 flasks with hypertonic citrate as previously described ( ).

Article Title: The pregnancy hormones human chorionic gonadotropin and progesterone induce human embryonic stem cell proliferation and differentiation into neuroectodermal rosettes
Article Snippet: The hESC colonies were grown in T25 flasks in a special ES cell-growth medium (78.5% DMEM-F12, 20% KOSR, 1% NEAA, 1 mM L-glutamine, 0.1 mM 2-mercaptoethanol) for 4 days with daily replacement of media to form ESC aggregates.

Knock-Out:

Article Title: Differential Processing of Amyloid-? Precursor Protein Directs Human Embryonic Stem Cell Proliferation and Differentiation into Neuronal Precursor Cells *
Article Snippet: .. The hESC colonies were grown in T25 flasks in a special embryonic stem cell growth medium (78.5% DMEM-F-12, 20% Knock-out Serum Replacer, 1% non-essential amino acids, 1 m m l -glutamine, and 0.1 m m 2-mercaptoethanol) for 4 days with daily replacement of medium to form embryonic stem cell aggregates. .. Cells were then adhered to the culture surface where they formed monolayer colonies in a chemically defined neural induction medium (32.6% F-12 (Invitrogen), 65.2% DMEM, 1% N2 supplement (Invitrogen), 1% non-essential amino acids, 0.2% 1 mg/ml heparin (Sigma-Aldrich), and 10 ng/ml basic fibroblast growth factor).

Transplantation Assay:

Article Title: Human Neural Stem Cell Transplantation Provides Long-Term Restoration of Neuronal Plasticity in the Irradiated Hippocampus
Article Snippet: Paragraph title: Transplantation Surgery ... The hNSCs were maintained as a monolayer in T25 flasks (Corning, Fisher Scientific, Pittsburgh, PA, USA) in neural expansion media (EMD-Millipore), expressed stem cells markers (Sox2, nestin), and displayed multilineage potential, as described previously ( ).

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    Fisher Scientific t25 flasks
    T25 Flasks, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Fisher Scientific
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    92
    Fisher Scientific t25 non tissue culture treated flasks
    T25 Non Tissue Culture Treated Flasks, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 non tissue culture treated flasks/product/Fisher Scientific
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t25 non tissue culture treated flasks - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    92
    Fisher Scientific t25 flask
    T25 Flask, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Fisher Scientific
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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