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Corning Life Sciences t25 flasks
T25 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flasks/product/Corning Life Sciences
Average 92 stars, based on 43 article reviews
Price from $9.99 to $1999.99
t25 flasks - by Bioz Stars, 2020-08
92/100 stars

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Cell Culture:

Article Title: Antibacterial activity of ceramide and ceramide analogs against pathogenic Neisseria
Article Snippet: .. HBMEC were cultured in T25 flasks (Corning Costar Corporation, Cambridge, MA, USA). .. The embryonic kidney cell line HEK293T and the hepatocellular carcinoma cell line HepG2 were purchased from the American Type Culture Collection, ATCC® CRL-3216™ and ATCC® HB-8065™ , respectively.

Purification:

Article Title: Macrophage colony-stimulating factor and its receptor signaling augment glycated albumin-induced retinal microglial inflammation in vitro
Article Snippet: .. GA-Induced Retinal Microglial Activation Purified microglial cells were seeded into T25 flasks. .. After 48 h of growth, the cells were incubated with serum-free DMEM/F-12 containing 0, 10, 50, 100, or 250 μg/mL human GA for 24 h. The GA used in this study (glycated human serum albumin from Sigma-Aldrich) was made less than 1 week prior as described by Baynes et al. [ ], and contained 2.7-3.5 mol of fructoselysine per mol of albumin.

Activation Assay:

Article Title: Macrophage colony-stimulating factor and its receptor signaling augment glycated albumin-induced retinal microglial inflammation in vitro
Article Snippet: .. GA-Induced Retinal Microglial Activation Purified microglial cells were seeded into T25 flasks. .. After 48 h of growth, the cells were incubated with serum-free DMEM/F-12 containing 0, 10, 50, 100, or 250 μg/mL human GA for 24 h. The GA used in this study (glycated human serum albumin from Sigma-Aldrich) was made less than 1 week prior as described by Baynes et al. [ ], and contained 2.7-3.5 mol of fructoselysine per mol of albumin.

Incubation:

Article Title: Contribution of HIF-1 and drug penetrance to oxaliplatin resistance in hypoxic colorectal cancer cells
Article Snippet: .. Cell viability and clonogenicity Cells were plated either in T25 flasks (5 ml media, Corning, Amsterdam, The Netherlands) for clonogenic assays or in 96-well plates (100 μ l media per well, Falcon, via Scientific Laboratory Supplies, Nottingham, UK), and were allowed to adhere for 16 h before incubation in standard atmospheric (5% CO2 /air) or hypoxic conditions for 8 h before drug treatment. .. Cells were maintained in hypoxic or standard atmospheric conditions and drugs administered for 16 h. Media were replaced and cells incubated under normoxia for 6 days before SRB assay as previously described , and the concentration that reduced growth by 50% (IC50 ) was determined.

other:

Article Title: Identification and Gene Expression Analysis of a Large Family of Transmembrane Kinases Related to the Gal/GalNAc Lectin in Entamoeba histolytica †
Article Snippet: For growth curve analysis, amebae were grown until they became nonadherent but still viable (144 h), seeded into T25 flasks with 300,000 ameba per flask (Corning Life Sciences, Corning, NY), and grown for 12 to 144 h.

Modification:

Article Title: Residues contributing to drug transport by ABCG2 are localised to multiple drug-binding pockets
Article Snippet: .. Unless stated otherwise, human embryonic kidney cells (HEK293T) were maintained in T25 flasks (Corning) at 37°C, 5% CO2 in Dulbecco's Modified Eagle Medium (DMEM, 4500 mg l -glucose, l -glutamine, sodium pyruvate, and sodium bicarbonate) supplemented with 10% (v/v) foetal calf serum (FCS; Gibco), 100 units/ml penicillin and 100 µg/ml streptomycin (Invitrogen). .. The cells were routinely monitored for their morphology and confluency and at 80–90% confluency the medium was removed and the cells were washed once with 2 ml of sterile phosphate-buffered saline (PBS) and then incubated (37°C) with 0.3 ml of trypsin/EDTA (Invitrogen) for 1–2 min to detach cells from the growing surface.

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    Corning Life Sciences t25 flasks
    T25 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Corning Life Sciences
    Average 92 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    91
    Corning Life Sciences t25 tissue culture flask
    T25 Tissue Culture Flask, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 tissue culture flask/product/Corning Life Sciences
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t25 tissue culture flask - by Bioz Stars, 2020-08
    91/100 stars
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    91
    Corning Life Sciences plastic closed cap t25 flasks
    Plastic Closed Cap T25 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plastic closed cap t25 flasks/product/Corning Life Sciences
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plastic closed cap t25 flasks - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

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