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Boster Bio t25 flasks
T25 Flasks, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flasks/product/Boster Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t25 flasks - by Bioz Stars, 2020-04
86/100 stars

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Centrifugation:

Article Title: Adipose-derived stem cells from the breast
Article Snippet: [ ] After digestion with collagenase I (Invitrogen, Carlsbad, USA), centrifugation, and filtration, the obtained cell fractions were cultured with Dulbecco's Modified Eagle Medium (DMEM)/F12 (Boster Biotech, Wuhan, China) supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, USA), 100 U/ml of penicillin, and 100 mg/ml of streptomycin (Boster Biotech, Wuhan, China) at 37°C in the presence of 5% CO2 for 48 h. Then, the non-adherent fractions were removed and the remaining cells were washed twice with phosphate-buffered saline (PBS; Boster Biotech, Wuhan, China). .. When 80% confluence was reached, the cells were digested and reseeded into T25 flasks (Boster Biotech, Wuhan, China) at ratio of 1:2.

Filtration:

Article Title: Adipose-derived stem cells from the breast
Article Snippet: [ ] After digestion with collagenase I (Invitrogen, Carlsbad, USA), centrifugation, and filtration, the obtained cell fractions were cultured with Dulbecco's Modified Eagle Medium (DMEM)/F12 (Boster Biotech, Wuhan, China) supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, USA), 100 U/ml of penicillin, and 100 mg/ml of streptomycin (Boster Biotech, Wuhan, China) at 37°C in the presence of 5% CO2 for 48 h. Then, the non-adherent fractions were removed and the remaining cells were washed twice with phosphate-buffered saline (PBS; Boster Biotech, Wuhan, China). .. When 80% confluence was reached, the cells were digested and reseeded into T25 flasks (Boster Biotech, Wuhan, China) at ratio of 1:2.

Isolation:

Article Title: Adipose-derived stem cells from the breast
Article Snippet: Paragraph title: Isolation of bADSCs ... When 80% confluence was reached, the cells were digested and reseeded into T25 flasks (Boster Biotech, Wuhan, China) at ratio of 1:2.

Cell Culture:

Article Title: Adipose-derived stem cells from the breast
Article Snippet: [ ] After digestion with collagenase I (Invitrogen, Carlsbad, USA), centrifugation, and filtration, the obtained cell fractions were cultured with Dulbecco's Modified Eagle Medium (DMEM)/F12 (Boster Biotech, Wuhan, China) supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, USA), 100 U/ml of penicillin, and 100 mg/ml of streptomycin (Boster Biotech, Wuhan, China) at 37°C in the presence of 5% CO2 for 48 h. Then, the non-adherent fractions were removed and the remaining cells were washed twice with phosphate-buffered saline (PBS; Boster Biotech, Wuhan, China). .. When 80% confluence was reached, the cells were digested and reseeded into T25 flasks (Boster Biotech, Wuhan, China) at ratio of 1:2.

Modification:

Article Title: Adipose-derived stem cells from the breast
Article Snippet: [ ] After digestion with collagenase I (Invitrogen, Carlsbad, USA), centrifugation, and filtration, the obtained cell fractions were cultured with Dulbecco's Modified Eagle Medium (DMEM)/F12 (Boster Biotech, Wuhan, China) supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, USA), 100 U/ml of penicillin, and 100 mg/ml of streptomycin (Boster Biotech, Wuhan, China) at 37°C in the presence of 5% CO2 for 48 h. Then, the non-adherent fractions were removed and the remaining cells were washed twice with phosphate-buffered saline (PBS; Boster Biotech, Wuhan, China). .. When 80% confluence was reached, the cells were digested and reseeded into T25 flasks (Boster Biotech, Wuhan, China) at ratio of 1:2.

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    Boster Bio t25 flasks
    T25 Flasks, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Boster Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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