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Bio Basic Canada t25 flasks
T25 Flasks, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flasks/product/Bio Basic Canada
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t25 flasks - by Bioz Stars, 2020-04
94/100 stars

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Transfection:

Article Title: Efficient proximity labeling in living cells and organisms with TurboID
Article Snippet: .. For preparation of lentiviruses, HEK 293T cells in T25 flasks (BioBasic) were transfected at ~60-70% confluency with the lentiviral vector pLX304 containing the gene of interest (2500 ng; ), and the lentiviral packaging plasmids pVSVG (250 ng; ) and Δ8.9 (2250 ng; ) with 30 μL Lipofectamine2000 in serum-free media for 3 hr, after which time the Lipofectamine-containing media was replaced with fresh serum-containing media. .. Approximately 60 hr after transfection, the cell medium containing the lentivirus was harvested and filtered through a 0.45-μm filter.

Selection:

Article Title: Efficient proximity labeling in living cells and organisms with TurboID
Article Snippet: For preparation of lentiviruses, HEK 293T cells in T25 flasks (BioBasic) were transfected at ~60-70% confluency with the lentiviral vector pLX304 containing the gene of interest (2500 ng; ), and the lentiviral packaging plasmids pVSVG (250 ng; ) and Δ8.9 (2250 ng; ) with 30 μL Lipofectamine2000 in serum-free media for 3 hr, after which time the Lipofectamine-containing media was replaced with fresh serum-containing media. .. To generate stable cell lines, HEK cells were then infected at ~50% confluency, followed by selection with 8 μg/mL blasticidin in growth medium for at least 7 days before further analysis ( and ).

Stable Transfection:

Article Title: Efficient proximity labeling in living cells and organisms with TurboID
Article Snippet: Paragraph title: Mammalian cell culture, transfection, and stable cell line generation ... For preparation of lentiviruses, HEK 293T cells in T25 flasks (BioBasic) were transfected at ~60-70% confluency with the lentiviral vector pLX304 containing the gene of interest (2500 ng; ), and the lentiviral packaging plasmids pVSVG (250 ng; ) and Δ8.9 (2250 ng; ) with 30 μL Lipofectamine2000 in serum-free media for 3 hr, after which time the Lipofectamine-containing media was replaced with fresh serum-containing media.

Cell Culture:

Article Title: Efficient proximity labeling in living cells and organisms with TurboID
Article Snippet: Paragraph title: Mammalian cell culture, transfection, and stable cell line generation ... For preparation of lentiviruses, HEK 293T cells in T25 flasks (BioBasic) were transfected at ~60-70% confluency with the lentiviral vector pLX304 containing the gene of interest (2500 ng; ), and the lentiviral packaging plasmids pVSVG (250 ng; ) and Δ8.9 (2250 ng; ) with 30 μL Lipofectamine2000 in serum-free media for 3 hr, after which time the Lipofectamine-containing media was replaced with fresh serum-containing media.

Labeling:

Article Title: Efficient proximity labeling in living cells and organisms with TurboID
Article Snippet: Labeling was stopped by placing cells on ice and washing five times with PBS ( ). .. For preparation of lentiviruses, HEK 293T cells in T25 flasks (BioBasic) were transfected at ~60-70% confluency with the lentiviral vector pLX304 containing the gene of interest (2500 ng; ), and the lentiviral packaging plasmids pVSVG (250 ng; ) and Δ8.9 (2250 ng; ) with 30 μL Lipofectamine2000 in serum-free media for 3 hr, after which time the Lipofectamine-containing media was replaced with fresh serum-containing media.

Infection:

Article Title: Efficient proximity labeling in living cells and organisms with TurboID
Article Snippet: For preparation of lentiviruses, HEK 293T cells in T25 flasks (BioBasic) were transfected at ~60-70% confluency with the lentiviral vector pLX304 containing the gene of interest (2500 ng; ), and the lentiviral packaging plasmids pVSVG (250 ng; ) and Δ8.9 (2250 ng; ) with 30 μL Lipofectamine2000 in serum-free media for 3 hr, after which time the Lipofectamine-containing media was replaced with fresh serum-containing media. .. To generate stable cell lines, HEK cells were then infected at ~50% confluency, followed by selection with 8 μg/mL blasticidin in growth medium for at least 7 days before further analysis ( and ).

Expressing:

Article Title: Efficient proximity labeling in living cells and organisms with TurboID
Article Snippet: TurboID and miniTurbo expressing-cells were typically labeled by supplementing 50 or 500 μM biotin for 10 min, approximately 36 hr after transfection; for longer time-points, labeling was initiated between 18-35 hr after transfection. .. For preparation of lentiviruses, HEK 293T cells in T25 flasks (BioBasic) were transfected at ~60-70% confluency with the lentiviral vector pLX304 containing the gene of interest (2500 ng; ), and the lentiviral packaging plasmids pVSVG (250 ng; ) and Δ8.9 (2250 ng; ) with 30 μL Lipofectamine2000 in serum-free media for 3 hr, after which time the Lipofectamine-containing media was replaced with fresh serum-containing media.

Plasmid Preparation:

Article Title: Efficient proximity labeling in living cells and organisms with TurboID
Article Snippet: .. For preparation of lentiviruses, HEK 293T cells in T25 flasks (BioBasic) were transfected at ~60-70% confluency with the lentiviral vector pLX304 containing the gene of interest (2500 ng; ), and the lentiviral packaging plasmids pVSVG (250 ng; ) and Δ8.9 (2250 ng; ) with 30 μL Lipofectamine2000 in serum-free media for 3 hr, after which time the Lipofectamine-containing media was replaced with fresh serum-containing media. .. Approximately 60 hr after transfection, the cell medium containing the lentivirus was harvested and filtered through a 0.45-μm filter.

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    Bio Basic Canada t25 flasks
    T25 Flasks, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Bio Basic Canada
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    94/100 stars
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