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Avantor t25 flasks
T25 Flasks, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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t25 flasks - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

DNA Extraction:

Article Title: A distinct epigenetic profile distinguishes stenotic from non-inflamed fibroblasts in the ileal mucosa of Crohn’s disease patients
Article Snippet: The fibroblasts were then plated in T25 flasks, after which samples in later passages were transferred to T75 flasks (VWR, Pennsylvania, USA; Tissue Culture Flasks 25 cm2 & 75 cm2) to allow for further expansion. .. For DNA isolation, fibroblasts were stored in PBS at -80°C, while cells for RNA isolation were stored in RNAlater (Thermo Fisher Scientific, Waltham, Massachusetts, USA) at 4°C overnight, after which the samples were transferred to -80°C as per manufacturer’s protocol.

RNA Extraction:

Article Title: Identification of cell-specific patterns of reference gene stability in quantitative reverse-transcriptase polymerase chain reaction studies of embryonic, placental and neural stem models of prenatal ethanol exposure
Article Snippet: Cells were cultured in T25 flasks, with a Parafilm (VWR) sealed lid to prevent ethanol evaporation. .. Neurospheres received fresh medium every 2–3 days while the growth medium for ES and TS cell cultures were replenished every 48 h. On Day 5 of treatment, cells were collected and RNA extraction was performed.

Article Title: Delta-Like 1 Homolog (Dlk1): A Marker for Rhabdomyosarcomas Implicated in Skeletal Muscle Regeneration
Article Snippet: C2C12 Cell Differentiation Assay DLK1-C2C12 and C2C12 control cells were seeded (2000 cells/cm2 ) in 6-well plates (Nunclon™, VWR, Rødovre, Denmark), T25 flasks (Nunclon™, VWR, Rødovre, Denmark) or in LabTek©CC2 4-well chamber slides (Nunclon™) and grown in proliferation medium: DMEM/high glucose (Gibco, Lifetechnologies, Taastrup, Denmark), 10% FBS (Gibco) and 1% Penicillin/Streptomycin (P/S) (Gibco) until 100% confluence (day 3), where after the cells were changed to differentiation medium: DMEM/high glucose with 2% Horse Serum (Gibco) and 1% P/S. .. Cells from 6-well plates were harvested for RNA extraction and qPCR in 1xNucleic Acid Purification Lysis Solution (Applied Biosystems).

RNA Sequencing Assay:

Article Title: A distinct epigenetic profile distinguishes stenotic from non-inflamed fibroblasts in the ileal mucosa of Crohn’s disease patients
Article Snippet: The fibroblasts were then plated in T25 flasks, after which samples in later passages were transferred to T75 flasks (VWR, Pennsylvania, USA; Tissue Culture Flasks 25 cm2 & 75 cm2) to allow for further expansion. .. Eighteen DNA samples were isolated from 10 unique patients for the methylation experiment, whereas 25 RNA samples were isolated from 15 unique patients for the RNA sequencing experiment.

Cell Differentiation:

Article Title: Delta-Like 1 Homolog (Dlk1): A Marker for Rhabdomyosarcomas Implicated in Skeletal Muscle Regeneration
Article Snippet: .. C2C12 Cell Differentiation Assay DLK1-C2C12 and C2C12 control cells were seeded (2000 cells/cm2 ) in 6-well plates (Nunclon™, VWR, Rødovre, Denmark), T25 flasks (Nunclon™, VWR, Rødovre, Denmark) or in LabTek©CC2 4-well chamber slides (Nunclon™) and grown in proliferation medium: DMEM/high glucose (Gibco, Lifetechnologies, Taastrup, Denmark), 10% FBS (Gibco) and 1% Penicillin/Streptomycin (P/S) (Gibco) until 100% confluence (day 3), where after the cells were changed to differentiation medium: DMEM/high glucose with 2% Horse Serum (Gibco) and 1% P/S. .. Cells from 6-well plates were harvested for RNA extraction and qPCR in 1xNucleic Acid Purification Lysis Solution (Applied Biosystems).

Methylation:

Article Title: A distinct epigenetic profile distinguishes stenotic from non-inflamed fibroblasts in the ileal mucosa of Crohn’s disease patients
Article Snippet: The fibroblasts were then plated in T25 flasks, after which samples in later passages were transferred to T75 flasks (VWR, Pennsylvania, USA; Tissue Culture Flasks 25 cm2 & 75 cm2) to allow for further expansion. .. Eighteen DNA samples were isolated from 10 unique patients for the methylation experiment, whereas 25 RNA samples were isolated from 15 unique patients for the RNA sequencing experiment.

Multiple Displacement Amplification:

Article Title: FGFR1 cleavage and nuclear translocation regulates breast cancer cell behavior
Article Snippet: .. Subcellular fractionation MCF-7 and MDA-MB-231 cells were plated in T25 flasks (VWR International), and subcellular fractionation was performed using a subcellular protein fractionation kit (PerkinElmer). .. In the case of FGF-10 and/or GrB treatment, fractionation was performed using a different kit (Universal Magnetic Co-IP kit; Active Motif) because of the discontinuation of the previous kit.

Isolation:

Article Title: A distinct epigenetic profile distinguishes stenotic from non-inflamed fibroblasts in the ileal mucosa of Crohn’s disease patients
Article Snippet: The fibroblasts were then plated in T25 flasks, after which samples in later passages were transferred to T75 flasks (VWR, Pennsylvania, USA; Tissue Culture Flasks 25 cm2 & 75 cm2) to allow for further expansion. .. For DNA isolation, fibroblasts were stored in PBS at -80°C, while cells for RNA isolation were stored in RNAlater (Thermo Fisher Scientific, Waltham, Massachusetts, USA) at 4°C overnight, after which the samples were transferred to -80°C as per manufacturer’s protocol.

Co-Immunoprecipitation Assay:

Article Title: FGFR1 cleavage and nuclear translocation regulates breast cancer cell behavior
Article Snippet: Subcellular fractionation MCF-7 and MDA-MB-231 cells were plated in T25 flasks (VWR International), and subcellular fractionation was performed using a subcellular protein fractionation kit (PerkinElmer). .. In the case of FGF-10 and/or GrB treatment, fractionation was performed using a different kit (Universal Magnetic Co-IP kit; Active Motif) because of the discontinuation of the previous kit.

Cell Culture:

Article Title: High Relaxivity Gd(III)–DNA Gold Nanostars: Investigation of Shape Effects on Proton Relaxation
Article Snippet: Paragraph title: Cell Culture ... Cells were grown in T25 flasks (VWR) at 37 °C and 5% CO2 .

Article Title: Identification of cell-specific patterns of reference gene stability in quantitative reverse-transcriptase polymerase chain reaction studies of embryonic, placental and neural stem models of prenatal ethanol exposure
Article Snippet: .. Cells were cultured in T25 flasks, with a Parafilm (VWR) sealed lid to prevent ethanol evaporation. .. Dosing for ethanol followed previously published studies , and utilized 60 mg/dL (13 mM), 120 mg/dL (26 mM), and 320 mg/dL (70 mM) of ethanol (Sigma).

Article Title: Enhanced HER2 Degradation in Breast Cancer Cells by Lysosome-Targeting Gold Nanoconstructs
Article Snippet: .. The cells were cultured at 37 C with 5% CO2 and plated in T25 flasks (VWR) with aforementioned media. .. After incubation of cells (1×104 cells/ml) with HApt-AuNS in a 96-well plate, the cell viability was measured using MTS assay solution (Promega).

Article Title: Alcohol induced epigenetic alterations to developmentally crucial genes regulating neural stemness and differentiation
Article Snippet: .. Neurospheres were cultured in T25 flasks, with a parafilm (VWR) sealed lid to prevent ethanol evaporation. .. Dosing for ethanol followed previously published studies , and utilized 60 mg/dL (13mM), 120 mg/dL (26mM), and 320 mg/dL (70mM) of ethanol (Sigma).

Article Title: Direct Observation of Nanoparticle-Cancer Cell Nucleus Interactions
Article Snippet: .. The cells were cultured at 37 °C with 5% CO2 and plated in T25 flasks (VWR) with aforementioned media. .. HeLa cells (2 × 104 cells/coverslip) were plated on poly-L-Lysine treated coverslips and maintained with Dulbecco modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS).

Evaporation:

Article Title: Identification of cell-specific patterns of reference gene stability in quantitative reverse-transcriptase polymerase chain reaction studies of embryonic, placental and neural stem models of prenatal ethanol exposure
Article Snippet: .. Cells were cultured in T25 flasks, with a Parafilm (VWR) sealed lid to prevent ethanol evaporation. .. Dosing for ethanol followed previously published studies , and utilized 60 mg/dL (13 mM), 120 mg/dL (26 mM), and 320 mg/dL (70 mM) of ethanol (Sigma).

Article Title: Alcohol induced epigenetic alterations to developmentally crucial genes regulating neural stemness and differentiation
Article Snippet: .. Neurospheres were cultured in T25 flasks, with a parafilm (VWR) sealed lid to prevent ethanol evaporation. .. Dosing for ethanol followed previously published studies , and utilized 60 mg/dL (13mM), 120 mg/dL (26mM), and 320 mg/dL (70mM) of ethanol (Sigma).

Real-time Polymerase Chain Reaction:

Article Title: Delta-Like 1 Homolog (Dlk1): A Marker for Rhabdomyosarcomas Implicated in Skeletal Muscle Regeneration
Article Snippet: C2C12 Cell Differentiation Assay DLK1-C2C12 and C2C12 control cells were seeded (2000 cells/cm2 ) in 6-well plates (Nunclon™, VWR, Rødovre, Denmark), T25 flasks (Nunclon™, VWR, Rødovre, Denmark) or in LabTek©CC2 4-well chamber slides (Nunclon™) and grown in proliferation medium: DMEM/high glucose (Gibco, Lifetechnologies, Taastrup, Denmark), 10% FBS (Gibco) and 1% Penicillin/Streptomycin (P/S) (Gibco) until 100% confluence (day 3), where after the cells were changed to differentiation medium: DMEM/high glucose with 2% Horse Serum (Gibco) and 1% P/S. .. Cells from 6-well plates were harvested for RNA extraction and qPCR in 1xNucleic Acid Purification Lysis Solution (Applied Biosystems).

Passaging:

Article Title: A distinct epigenetic profile distinguishes stenotic from non-inflamed fibroblasts in the ileal mucosa of Crohn’s disease patients
Article Snippet: Paragraph title: Passaging and harvesting of fibroblasts ... The fibroblasts were then plated in T25 flasks, after which samples in later passages were transferred to T75 flasks (VWR, Pennsylvania, USA; Tissue Culture Flasks 25 cm2 & 75 cm2) to allow for further expansion.

Protein Extraction:

Article Title: Delta-Like 1 Homolog (Dlk1): A Marker for Rhabdomyosarcomas Implicated in Skeletal Muscle Regeneration
Article Snippet: C2C12 Cell Differentiation Assay DLK1-C2C12 and C2C12 control cells were seeded (2000 cells/cm2 ) in 6-well plates (Nunclon™, VWR, Rødovre, Denmark), T25 flasks (Nunclon™, VWR, Rødovre, Denmark) or in LabTek©CC2 4-well chamber slides (Nunclon™) and grown in proliferation medium: DMEM/high glucose (Gibco, Lifetechnologies, Taastrup, Denmark), 10% FBS (Gibco) and 1% Penicillin/Streptomycin (P/S) (Gibco) until 100% confluence (day 3), where after the cells were changed to differentiation medium: DMEM/high glucose with 2% Horse Serum (Gibco) and 1% P/S. .. T25 flasks were harvested for protein extraction and western blotting.

Fractionation:

Article Title: FGFR1 cleavage and nuclear translocation regulates breast cancer cell behavior
Article Snippet: .. Subcellular fractionation MCF-7 and MDA-MB-231 cells were plated in T25 flasks (VWR International), and subcellular fractionation was performed using a subcellular protein fractionation kit (PerkinElmer). .. In the case of FGF-10 and/or GrB treatment, fractionation was performed using a different kit (Universal Magnetic Co-IP kit; Active Motif) because of the discontinuation of the previous kit.

Lysis:

Article Title: Delta-Like 1 Homolog (Dlk1): A Marker for Rhabdomyosarcomas Implicated in Skeletal Muscle Regeneration
Article Snippet: C2C12 Cell Differentiation Assay DLK1-C2C12 and C2C12 control cells were seeded (2000 cells/cm2 ) in 6-well plates (Nunclon™, VWR, Rødovre, Denmark), T25 flasks (Nunclon™, VWR, Rødovre, Denmark) or in LabTek©CC2 4-well chamber slides (Nunclon™) and grown in proliferation medium: DMEM/high glucose (Gibco, Lifetechnologies, Taastrup, Denmark), 10% FBS (Gibco) and 1% Penicillin/Streptomycin (P/S) (Gibco) until 100% confluence (day 3), where after the cells were changed to differentiation medium: DMEM/high glucose with 2% Horse Serum (Gibco) and 1% P/S. .. Cells from 6-well plates were harvested for RNA extraction and qPCR in 1xNucleic Acid Purification Lysis Solution (Applied Biosystems).

Modification:

Article Title: High Relaxivity Gd(III)–DNA Gold Nanostars: Investigation of Shape Effects on Proton Relaxation
Article Snippet: Pancreatic cancer cells (PANC-1, ATCC) were maintained in Dulbecco’s modified Eagle medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (Invitrogen). .. Cells were grown in T25 flasks (VWR) at 37 °C and 5% CO2 .

Western Blot:

Article Title: Delta-Like 1 Homolog (Dlk1): A Marker for Rhabdomyosarcomas Implicated in Skeletal Muscle Regeneration
Article Snippet: C2C12 Cell Differentiation Assay DLK1-C2C12 and C2C12 control cells were seeded (2000 cells/cm2 ) in 6-well plates (Nunclon™, VWR, Rødovre, Denmark), T25 flasks (Nunclon™, VWR, Rødovre, Denmark) or in LabTek©CC2 4-well chamber slides (Nunclon™) and grown in proliferation medium: DMEM/high glucose (Gibco, Lifetechnologies, Taastrup, Denmark), 10% FBS (Gibco) and 1% Penicillin/Streptomycin (P/S) (Gibco) until 100% confluence (day 3), where after the cells were changed to differentiation medium: DMEM/high glucose with 2% Horse Serum (Gibco) and 1% P/S. .. T25 flasks were harvested for protein extraction and western blotting.

Purification:

Article Title: Delta-Like 1 Homolog (Dlk1): A Marker for Rhabdomyosarcomas Implicated in Skeletal Muscle Regeneration
Article Snippet: C2C12 Cell Differentiation Assay DLK1-C2C12 and C2C12 control cells were seeded (2000 cells/cm2 ) in 6-well plates (Nunclon™, VWR, Rødovre, Denmark), T25 flasks (Nunclon™, VWR, Rødovre, Denmark) or in LabTek©CC2 4-well chamber slides (Nunclon™) and grown in proliferation medium: DMEM/high glucose (Gibco, Lifetechnologies, Taastrup, Denmark), 10% FBS (Gibco) and 1% Penicillin/Streptomycin (P/S) (Gibco) until 100% confluence (day 3), where after the cells were changed to differentiation medium: DMEM/high glucose with 2% Horse Serum (Gibco) and 1% P/S. .. Cells from 6-well plates were harvested for RNA extraction and qPCR in 1xNucleic Acid Purification Lysis Solution (Applied Biosystems).