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Sarstedt t25 flask
T25 Flask, supplied by Sarstedt, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flask/product/Sarstedt
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
t25 flask - by Bioz Stars, 2020-04
92/100 stars

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Related Articles

Infection:

Article Title: Actinobacillus pleuropneumoniae induces SJPL cell cycle arrest in G2/M-phase and inhibits porcine reproductive and respiratory syndrome virus replication
Article Snippet: .. Briefly, a T25 flask (Sarstedt Inc.) of confluent SJPL cells (0.5 × 106 cells) were infected or not with IAF-Klop PRRSV strain for 4 h (multiplicity of infection (MOI): 0.5) and then treated with AppΔapxIC ΔapxIIC culture supernatant or left untreated for 18 h. These conditions are known to block PRRSV replication [ ]. .. Cells were washed twice with Dulbecco's phosphate-buffered saline (DPBS; Life Technologies) and 500 μL of lysis buffer was added (20 mM MOPS (pH 7.0; Sigma-Aldrich), 2 mM EGTA (Sigma-Aldrich), 5 mM EDTA (Fisher Scientific, Fair Lawn, NJ, USA), 30 mM sodium fluoride (Sigma-Aldrich), 60 mM β-glycerophosphate (pH 7.2; Sigma-Aldrich), 20 mM sodium pyrophosphate (Sigma-Aldrich), 1 mM sodium orthovanadate (Sigma-Aldrich), 1 % (v/v) Triton X-100 (Sigma-Aldrich) and one protease inhibitor cocktail tablet Complete Mini EDTA-free (Roche Diagnostics GmbH, Mannheim, Germany), pH 7.2).

Article Title: Enhancing the Oncolytic Activity of CD133-Targeted Measles Virus: Receptor Extension or Chimerism with Vesicular Stomatitis Virus Are Most Effective
Article Snippet: Orthotopic Glioma Model 1 × 105 NCH644 tumor sphere cells either uninfected or infected at an MOI of 0.5 were dissociated and stereotactically implanted in 5 µl of PBS into the corpus striatum of the right hemisphere (1.7 mm lateral, 0.5 mm rostral to bregma at 3 mm depth) of 6- to 8-week-old NOD/SCID mice. .. 2 × 105 single cells were cultured in T25 flask (Sarstedt, Germany) in DMEM/F-12 medium containing 20% BIT serum-free supplement, basic fibroblast growth factor, and epidermal growth factor at 20 ng/mL (all Provitro, Germany).

Article Title: A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum
Article Snippet: .. Late-stationary-phase promastigotes transfected with the L. infantum genomic DNA cosmid library were added to 5 × 107 BMMs at a multiplicity of infection of 8:1 in a vented T25 flask (Sarstedt). .. At 24 h postinfection, 160 μg ml−1 sodium stibogluconate (SbV ) or 35 μM miltefosine (IC50 ) was added.

Transfection:

Article Title: A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum
Article Snippet: .. Late-stationary-phase promastigotes transfected with the L. infantum genomic DNA cosmid library were added to 5 × 107 BMMs at a multiplicity of infection of 8:1 in a vented T25 flask (Sarstedt). .. At 24 h postinfection, 160 μg ml−1 sodium stibogluconate (SbV ) or 35 μM miltefosine (IC50 ) was added.

Selection:

Article Title: A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum
Article Snippet: Paragraph title: Drug selection and recovery of cosmid DNA. ... Late-stationary-phase promastigotes transfected with the L. infantum genomic DNA cosmid library were added to 5 × 107 BMMs at a multiplicity of infection of 8:1 in a vented T25 flask (Sarstedt).

Sample Prep:

Article Title: Actinobacillus pleuropneumoniae induces SJPL cell cycle arrest in G2/M-phase and inhibits porcine reproductive and respiratory syndrome virus replication
Article Snippet: Protein profiling of SJPL cells Sample preparation was performed as described by Auger and collaborators [ ]. .. Briefly, a T25 flask (Sarstedt Inc.) of confluent SJPL cells (0.5 × 106 cells) were infected or not with IAF-Klop PRRSV strain for 4 h (multiplicity of infection (MOI): 0.5) and then treated with AppΔapxIC ΔapxIIC culture supernatant or left untreated for 18 h. These conditions are known to block PRRSV replication [ ].

Modification:

Article Title: A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes
Article Snippet: .. Luciferase Assay Mink Lung epithelial cells (MLECs—a gift from Prof D Rifkin, New York University) were maintained in T25 flask (Sarstedt) containing 5 ml of Dulbecco’s modified Eagle’s Media (DMEM) (Sigma-Aldrich) supplemented with 10% of heat inactivated fetal calf serum (Sigma-Aldrich), penicillin (100 U/ml), streptomycin (100 U/ml), L-glutamine and 200 μg/ml, G418 (Sigma-Aldrich). .. For use in luciferase measurements cells were used at a concentration of 1.6x106 cells/ml.

Protease Inhibitor:

Article Title: Actinobacillus pleuropneumoniae induces SJPL cell cycle arrest in G2/M-phase and inhibits porcine reproductive and respiratory syndrome virus replication
Article Snippet: Briefly, a T25 flask (Sarstedt Inc.) of confluent SJPL cells (0.5 × 106 cells) were infected or not with IAF-Klop PRRSV strain for 4 h (multiplicity of infection (MOI): 0.5) and then treated with AppΔapxIC ΔapxIIC culture supernatant or left untreated for 18 h. These conditions are known to block PRRSV replication [ ]. .. Cells were washed twice with Dulbecco's phosphate-buffered saline (DPBS; Life Technologies) and 500 μL of lysis buffer was added (20 mM MOPS (pH 7.0; Sigma-Aldrich), 2 mM EGTA (Sigma-Aldrich), 5 mM EDTA (Fisher Scientific, Fair Lawn, NJ, USA), 30 mM sodium fluoride (Sigma-Aldrich), 60 mM β-glycerophosphate (pH 7.2; Sigma-Aldrich), 20 mM sodium pyrophosphate (Sigma-Aldrich), 1 mM sodium orthovanadate (Sigma-Aldrich), 1 % (v/v) Triton X-100 (Sigma-Aldrich) and one protease inhibitor cocktail tablet Complete Mini EDTA-free (Roche Diagnostics GmbH, Mannheim, Germany), pH 7.2).

Diffusion-based Assay:

Article Title: Enhancing the Oncolytic Activity of CD133-Targeted Measles Virus: Receptor Extension or Chimerism with Vesicular Stomatitis Virus Are Most Effective
Article Snippet: After pausing for 5 min to allow the diffusion of the carrier fluid into the brain parenchyma, the injection needle was slowly extracted. .. 2 × 105 single cells were cultured in T25 flask (Sarstedt, Germany) in DMEM/F-12 medium containing 20% BIT serum-free supplement, basic fibroblast growth factor, and epidermal growth factor at 20 ng/mL (all Provitro, Germany).

Blocking Assay:

Article Title: Actinobacillus pleuropneumoniae induces SJPL cell cycle arrest in G2/M-phase and inhibits porcine reproductive and respiratory syndrome virus replication
Article Snippet: .. Briefly, a T25 flask (Sarstedt Inc.) of confluent SJPL cells (0.5 × 106 cells) were infected or not with IAF-Klop PRRSV strain for 4 h (multiplicity of infection (MOI): 0.5) and then treated with AppΔapxIC ΔapxIIC culture supernatant or left untreated for 18 h. These conditions are known to block PRRSV replication [ ]. .. Cells were washed twice with Dulbecco's phosphate-buffered saline (DPBS; Life Technologies) and 500 μL of lysis buffer was added (20 mM MOPS (pH 7.0; Sigma-Aldrich), 2 mM EGTA (Sigma-Aldrich), 5 mM EDTA (Fisher Scientific, Fair Lawn, NJ, USA), 30 mM sodium fluoride (Sigma-Aldrich), 60 mM β-glycerophosphate (pH 7.2; Sigma-Aldrich), 20 mM sodium pyrophosphate (Sigma-Aldrich), 1 mM sodium orthovanadate (Sigma-Aldrich), 1 % (v/v) Triton X-100 (Sigma-Aldrich) and one protease inhibitor cocktail tablet Complete Mini EDTA-free (Roche Diagnostics GmbH, Mannheim, Germany), pH 7.2).

Mouse Assay:

Article Title: Enhancing the Oncolytic Activity of CD133-Targeted Measles Virus: Receptor Extension or Chimerism with Vesicular Stomatitis Virus Are Most Effective
Article Snippet: Orthotopic Glioma Model 1 × 105 NCH644 tumor sphere cells either uninfected or infected at an MOI of 0.5 were dissociated and stereotactically implanted in 5 µl of PBS into the corpus striatum of the right hemisphere (1.7 mm lateral, 0.5 mm rostral to bregma at 3 mm depth) of 6- to 8-week-old NOD/SCID mice. .. 2 × 105 single cells were cultured in T25 flask (Sarstedt, Germany) in DMEM/F-12 medium containing 20% BIT serum-free supplement, basic fibroblast growth factor, and epidermal growth factor at 20 ng/mL (all Provitro, Germany).

Concentration Assay:

Article Title: A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes
Article Snippet: Luciferase Assay Mink Lung epithelial cells (MLECs—a gift from Prof D Rifkin, New York University) were maintained in T25 flask (Sarstedt) containing 5 ml of Dulbecco’s modified Eagle’s Media (DMEM) (Sigma-Aldrich) supplemented with 10% of heat inactivated fetal calf serum (Sigma-Aldrich), penicillin (100 U/ml), streptomycin (100 U/ml), L-glutamine and 200 μg/ml, G418 (Sigma-Aldrich). .. For use in luciferase measurements cells were used at a concentration of 1.6x106 cells/ml.

Article Title: A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum
Article Snippet: Late-stationary-phase promastigotes transfected with the L. infantum genomic DNA cosmid library were added to 5 × 107 BMMs at a multiplicity of infection of 8:1 in a vented T25 flask (Sarstedt). .. Late-stationary-phase promastigotes transfected with the L. infantum genomic DNA cosmid library were added to 5 × 107 BMMs at a multiplicity of infection of 8:1 in a vented T25 flask (Sarstedt).

Luciferase:

Article Title: A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes
Article Snippet: .. Luciferase Assay Mink Lung epithelial cells (MLECs—a gift from Prof D Rifkin, New York University) were maintained in T25 flask (Sarstedt) containing 5 ml of Dulbecco’s modified Eagle’s Media (DMEM) (Sigma-Aldrich) supplemented with 10% of heat inactivated fetal calf serum (Sigma-Aldrich), penicillin (100 U/ml), streptomycin (100 U/ml), L-glutamine and 200 μg/ml, G418 (Sigma-Aldrich). .. For use in luciferase measurements cells were used at a concentration of 1.6x106 cells/ml.

Cell Culture:

Article Title: Enhancing the Oncolytic Activity of CD133-Targeted Measles Virus: Receptor Extension or Chimerism with Vesicular Stomatitis Virus Are Most Effective
Article Snippet: .. 2 × 105 single cells were cultured in T25 flask (Sarstedt, Germany) in DMEM/F-12 medium containing 20% BIT serum-free supplement, basic fibroblast growth factor, and epidermal growth factor at 20 ng/mL (all Provitro, Germany). .. Cells were cultivated until spheres had formed, and a cell density of about 1 × 106 cells per T25 flask was reached.

Lysis:

Article Title: Actinobacillus pleuropneumoniae induces SJPL cell cycle arrest in G2/M-phase and inhibits porcine reproductive and respiratory syndrome virus replication
Article Snippet: Briefly, a T25 flask (Sarstedt Inc.) of confluent SJPL cells (0.5 × 106 cells) were infected or not with IAF-Klop PRRSV strain for 4 h (multiplicity of infection (MOI): 0.5) and then treated with AppΔapxIC ΔapxIIC culture supernatant or left untreated for 18 h. These conditions are known to block PRRSV replication [ ]. .. Cells were washed twice with Dulbecco's phosphate-buffered saline (DPBS; Life Technologies) and 500 μL of lysis buffer was added (20 mM MOPS (pH 7.0; Sigma-Aldrich), 2 mM EGTA (Sigma-Aldrich), 5 mM EDTA (Fisher Scientific, Fair Lawn, NJ, USA), 30 mM sodium fluoride (Sigma-Aldrich), 60 mM β-glycerophosphate (pH 7.2; Sigma-Aldrich), 20 mM sodium pyrophosphate (Sigma-Aldrich), 1 mM sodium orthovanadate (Sigma-Aldrich), 1 % (v/v) Triton X-100 (Sigma-Aldrich) and one protease inhibitor cocktail tablet Complete Mini EDTA-free (Roche Diagnostics GmbH, Mannheim, Germany), pH 7.2).

Ex Vivo:

Article Title: Enhancing the Oncolytic Activity of CD133-Targeted Measles Virus: Receptor Extension or Chimerism with Vesicular Stomatitis Virus Are Most Effective
Article Snippet: For ex vivo culturing of glioma spheres, the whole tumor was excised and enzymatically dissociated using the Miltenyi Brain tumor dissociation Kit according to the manufacturers’ instructions. .. 2 × 105 single cells were cultured in T25 flask (Sarstedt, Germany) in DMEM/F-12 medium containing 20% BIT serum-free supplement, basic fibroblast growth factor, and epidermal growth factor at 20 ng/mL (all Provitro, Germany).

Sonication:

Article Title: Actinobacillus pleuropneumoniae induces SJPL cell cycle arrest in G2/M-phase and inhibits porcine reproductive and respiratory syndrome virus replication
Article Snippet: Briefly, a T25 flask (Sarstedt Inc.) of confluent SJPL cells (0.5 × 106 cells) were infected or not with IAF-Klop PRRSV strain for 4 h (multiplicity of infection (MOI): 0.5) and then treated with AppΔapxIC ΔapxIIC culture supernatant or left untreated for 18 h. These conditions are known to block PRRSV replication [ ]. .. Sonication was performed at 180 Joules using an ultrasonic processor (Cole-Parmer, Vernin Hills, IL, USA) in order to lyse the cells.

Injection:

Article Title: Enhancing the Oncolytic Activity of CD133-Targeted Measles Virus: Receptor Extension or Chimerism with Vesicular Stomatitis Virus Are Most Effective
Article Snippet: Intracranial injection of virus was performed stereotactically in 5 µl PBS into the same coordinates. .. 2 × 105 single cells were cultured in T25 flask (Sarstedt, Germany) in DMEM/F-12 medium containing 20% BIT serum-free supplement, basic fibroblast growth factor, and epidermal growth factor at 20 ng/mL (all Provitro, Germany).

Cosmid DNA:

Article Title: A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum
Article Snippet: Paragraph title: Drug selection and recovery of cosmid DNA. ... Late-stationary-phase promastigotes transfected with the L. infantum genomic DNA cosmid library were added to 5 × 107 BMMs at a multiplicity of infection of 8:1 in a vented T25 flask (Sarstedt).

other:

Article Title: Enhancing the Oncolytic Activity of CD133-Targeted Measles Virus: Receptor Extension or Chimerism with Vesicular Stomatitis Virus Are Most Effective
Article Snippet: Cells were cultivated until spheres had formed, and a cell density of about 1 × 106 cells per T25 flask was reached.

Isolation:

Article Title: A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum
Article Snippet: Selected parasites were harvested for cosmid isolation. .. Late-stationary-phase promastigotes transfected with the L. infantum genomic DNA cosmid library were added to 5 × 107 BMMs at a multiplicity of infection of 8:1 in a vented T25 flask (Sarstedt).

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  • 95
    Sarstedt t25 flasks
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    T25 Flasks, supplied by Sarstedt, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Sarstedt
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    85
    Sarstedt • tissue culture flask t25
    <t>T25</t> cardboard cover design for AF calculation method. (a) Shows the cardboard cover design next to T25 flask. (b) and (c) show how cover is placed onto a T25 flask before photography.
    • Tissue Culture Flask T25, supplied by Sarstedt, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/• tissue culture flask t25/product/Sarstedt
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    • tissue culture flask t25 - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    Image Search Results


    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Journal: PLoS ONE

    Article Title: Cell colony counter called CoCoNut

    doi: 10.1371/journal.pone.0205823

    Figure Lengend Snippet: Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Article Snippet: The count for viable cells was used for plaiting cells in either T25 flasks (25 cm2 cell culture area; Sarstedt, Skanderborg, Denmark) with ventilated caps containing 6 ml complete medium or in 35 mm cell culture dishes (Sigma-Aldrich) with 3 ml complete medium, respectively.

    Techniques: Cell Culture

    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Journal: PLoS ONE

    Article Title: Cell colony counter called CoCoNut

    doi: 10.1371/journal.pone.0205823

    Figure Lengend Snippet: Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Article Snippet: The count for viable cells was used for plaiting cells in either T25 flasks (25 cm2 cell culture area; Sarstedt, Skanderborg, Denmark) with ventilated caps containing 6 ml complete medium or in 35 mm cell culture dishes (Sigma-Aldrich) with 3 ml complete medium, respectively.

    Techniques: Cell Culture

    T25 cardboard cover design for AF calculation method. (a) Shows the cardboard cover design next to T25 flask. (b) and (c) show how cover is placed onto a T25 flask before photography.

    Journal: MethodsX

    Article Title: Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines

    doi: 10.1016/j.mex.2014.11.002

    Figure Lengend Snippet: T25 cardboard cover design for AF calculation method. (a) Shows the cardboard cover design next to T25 flask. (b) and (c) show how cover is placed onto a T25 flask before photography.

    Article Snippet: • Tissue Culture Flask T25, with red vented cap (Sarstedt, Wexford, Ireland).

    Techniques:

    Correlation of AF method with cell counts using a haemocytometer. The average total cell count per T25 flask for each day of a four day growth curve is shown on the x -axis of each graph and the AF output average for the same T25 flask for each day is shown on the y -axis. For each graph error bars represent the standard deviation. (a) Shows a representative graph for OVCAR8. (b) Shows a representative graph for UPN251.

    Journal: MethodsX

    Article Title: Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines

    doi: 10.1016/j.mex.2014.11.002

    Figure Lengend Snippet: Correlation of AF method with cell counts using a haemocytometer. The average total cell count per T25 flask for each day of a four day growth curve is shown on the x -axis of each graph and the AF output average for the same T25 flask for each day is shown on the y -axis. For each graph error bars represent the standard deviation. (a) Shows a representative graph for OVCAR8. (b) Shows a representative graph for UPN251.

    Article Snippet: • Tissue Culture Flask T25, with red vented cap (Sarstedt, Wexford, Ireland).

    Techniques: Cell Counting, Standard Deviation