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SPL Life Sciences t25 flask
T25 Flask, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flask/product/SPL Life Sciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t25 flask - by Bioz Stars, 2020-04
94/100 stars

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Isolation:

Article Title: Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer
Article Snippet: Expansion and activation of NKL The isolated PBMCs from the first step were cultured in T25 flask (cat no. 70025; SPL Life Science, Pocheon, Korea) with 10 ml of NKL culture medium containing 10% autologous human plasma for a day or two (second step). .. The cells and supernatants in T25 flask were then transferred to T75 flask (cat no. 70075; SPL Life Science) and new NKL culture medium containing 10% autologous human plasma was added to the flask to make a final volume of 30 ml (third step).

Article Title: Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer
Article Snippet: .. Expansion and activation of NKL The isolated PBMCs from the first step were cultured in T25 flask (cat no. 70025; SPL Life Science, Pocheon, Korea) with 10 ml of NKL culture medium containing 10% autologous human plasma for a day or two (second step). .. The cells and supernatants in T25 flask were then transferred to T75 flask (cat no. 70075; SPL Life Science) and new NKL culture medium containing 10% autologous human plasma was added to the flask to make a final volume of 30 ml (third step).

Cell Culture:

Article Title: Voltage-Gated K+ Channel, Kv3.3 Is Involved in Hemin-Induced K562 Differentiation
Article Snippet: Paragraph title: 2.1. Cell culture and hemin-induced cell differentiation ... When sufficient growth was achieved, 1 x 105 cells were plated into a new T25 flask (SPL Life Sciences, Gyeonggi-do, Korea) and incubated with 50 μM hemin (Sigma, St. Louis, MO) to induce erythroid differentiation.

Article Title: Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer
Article Snippet: Expansion and activation of NKL The isolated PBMCs from the first step were cultured in T25 flask (cat no. 70025; SPL Life Science, Pocheon, Korea) with 10 ml of NKL culture medium containing 10% autologous human plasma for a day or two (second step). .. The cells and supernatants in T25 flask were then transferred to T75 flask (cat no. 70075; SPL Life Science) and new NKL culture medium containing 10% autologous human plasma was added to the flask to make a final volume of 30 ml (third step).

Article Title: Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer
Article Snippet: .. Expansion and activation of NKL The isolated PBMCs from the first step were cultured in T25 flask (cat no. 70025; SPL Life Science, Pocheon, Korea) with 10 ml of NKL culture medium containing 10% autologous human plasma for a day or two (second step). .. The cells and supernatants in T25 flask were then transferred to T75 flask (cat no. 70075; SPL Life Science) and new NKL culture medium containing 10% autologous human plasma was added to the flask to make a final volume of 30 ml (third step).

Cell Differentiation:

Article Title: Voltage-Gated K+ Channel, Kv3.3 Is Involved in Hemin-Induced K562 Differentiation
Article Snippet: Paragraph title: 2.1. Cell culture and hemin-induced cell differentiation ... When sufficient growth was achieved, 1 x 105 cells were plated into a new T25 flask (SPL Life Sciences, Gyeonggi-do, Korea) and incubated with 50 μM hemin (Sigma, St. Louis, MO) to induce erythroid differentiation.

Activation Assay:

Article Title: Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer
Article Snippet: Paragraph title: Expansion and activation of NKL ... The cells and supernatants in T25 flask were then transferred to T75 flask (cat no. 70075; SPL Life Science) and new NKL culture medium containing 10% autologous human plasma was added to the flask to make a final volume of 30 ml (third step).

Article Title: Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer
Article Snippet: .. Expansion and activation of NKL The isolated PBMCs from the first step were cultured in T25 flask (cat no. 70025; SPL Life Science, Pocheon, Korea) with 10 ml of NKL culture medium containing 10% autologous human plasma for a day or two (second step). .. The cells and supernatants in T25 flask were then transferred to T75 flask (cat no. 70075; SPL Life Science) and new NKL culture medium containing 10% autologous human plasma was added to the flask to make a final volume of 30 ml (third step).

Incubation:

Article Title: Voltage-Gated K+ Channel, Kv3.3 Is Involved in Hemin-Induced K562 Differentiation
Article Snippet: .. When sufficient growth was achieved, 1 x 105 cells were plated into a new T25 flask (SPL Life Sciences, Gyeonggi-do, Korea) and incubated with 50 μM hemin (Sigma, St. Louis, MO) to induce erythroid differentiation. .. Reverse transcription-polymerase chain reaction (RT-PCR) Total RNA was isolated using RNeasy Micro Kit (Quiagen, Valencia, CA) according to the manufacturer’s instructions.

Injection:

Article Title: Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer
Article Snippet: The cells and supernatants in T25 flask were then transferred to T75 flask (cat no. 70075; SPL Life Science) and new NKL culture medium containing 10% autologous human plasma was added to the flask to make a final volume of 30 ml (third step). .. After two or 3 days, the cells and supernatants in T175 flask were injected into gas-permeable culture bag (cat no. 1602502B; KOHJIN Bio) and 1000 mL of new NKL culture medium containing 0.5% autologous human plasma was added.

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  • 94
    SPL Life Sciences t25 flask
    T25 Flask, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/SPL Life Sciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    94
    SPL Life Sciences t25 flasks
    T25 Flasks, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/SPL Life Sciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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