Structured Review

Millipore t25 flask
T25 Flask, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flask/product/Millipore
Average 94 stars, based on 8 article reviews
Price from $9.99 to $1999.99
t25 flask - by Bioz Stars, 2020-07
94/100 stars

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Modification:

Article Title: Development and Characterization of Novel Porous 3D Alginate-Cockle Shell Powder Nanobiocomposite Bone Scaffold
Article Snippet: .. The cells from a single vial were seeded into a T25 culture flask containing Dulbecco's modified Eagle medium (DMEM: PAA E15-810) supplemented with 10% fetal bovine serum (FBS: Sigma F4135) and 1% penicillin/streptomycin (PAA P11-010) at 37°C temperature and 5% CO2 . .. The cells were detached using trypsin/EDTA (0.25% w/v trypsin/0.02% EDTA, Sigma), concentrated by centrifugation at 800 rpm for 5 minutes, and resuspended in fresh medium.

Article Title: Long-term Brillouin imaging of live cells with reduced absorption-mediated damage at 660 nm wavelength
Article Snippet: .. They were grown in T25 cell culture flasks in the standard 3T3 medium: Dulbecco’s modified Eagle medium (DMEM) supplemented with 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate (Sigma-Aldrich, catalog no. D5796). .. The DMEM formulation was supplemented with 10% (v/v) bovine calf serum (ATCC 30-2030), and 50 U/mL penicillin and 50 µg/ml streptomycin (Thermo Fisher, catalog no. 15070063).

Isolation:

Article Title: mir-124-5p Regulates Phagocytosis of Human Macrophages by Targeting the Actin Cytoskeleton via the ARP2/3 Complex
Article Snippet: .. Peripheral blood mononuclear cells were isolated by density gradient centrifugation using Histopaque®-1077 (Sigma) as described previously ( ) and 4–5 × 107 cells were seeded into T25 cell culture flasks and incubated 1 h in RPMI 1640 containing 1% human serum (Sigma) at 37°C and 5% CO2 . .. Non-adherent cells were removed by washing twice with PBS (Sigma).

Incubation:

Article Title: mir-124-5p Regulates Phagocytosis of Human Macrophages by Targeting the Actin Cytoskeleton via the ARP2/3 Complex
Article Snippet: .. Peripheral blood mononuclear cells were isolated by density gradient centrifugation using Histopaque®-1077 (Sigma) as described previously ( ) and 4–5 × 107 cells were seeded into T25 cell culture flasks and incubated 1 h in RPMI 1640 containing 1% human serum (Sigma) at 37°C and 5% CO2 . .. Non-adherent cells were removed by washing twice with PBS (Sigma).

Gradient Centrifugation:

Article Title: mir-124-5p Regulates Phagocytosis of Human Macrophages by Targeting the Actin Cytoskeleton via the ARP2/3 Complex
Article Snippet: .. Peripheral blood mononuclear cells were isolated by density gradient centrifugation using Histopaque®-1077 (Sigma) as described previously ( ) and 4–5 × 107 cells were seeded into T25 cell culture flasks and incubated 1 h in RPMI 1640 containing 1% human serum (Sigma) at 37°C and 5% CO2 . .. Non-adherent cells were removed by washing twice with PBS (Sigma).

Cell Culture:

Article Title: Hexane soluble extract of Mallotus philippensis (Lam.) Muell. Arg. root possesses anti-leukaemic activity
Article Snippet: .. HL-60 cells (0.1 × 106 /ml) were seeded in T25 cell culture flasks and exposed to increasing concentrations of M. philippensis hexane extract for 48 h. Hoechst 33258 (HO) and propidium iodide (PI, both Sigma, St Louis, MO) were added directly to the cells to final concentrations of 5 and 2 mg/ml, respectively. .. After incubation for 60 min at 37°C, cells were examined under fluorescence microscope (Axiovert, Zeiss) equipped with a filter and a camera.

Article Title: mir-124-5p Regulates Phagocytosis of Human Macrophages by Targeting the Actin Cytoskeleton via the ARP2/3 Complex
Article Snippet: .. Peripheral blood mononuclear cells were isolated by density gradient centrifugation using Histopaque®-1077 (Sigma) as described previously ( ) and 4–5 × 107 cells were seeded into T25 cell culture flasks and incubated 1 h in RPMI 1640 containing 1% human serum (Sigma) at 37°C and 5% CO2 . .. Non-adherent cells were removed by washing twice with PBS (Sigma).

Article Title: A Tumor-Promoting Phorbol Ester Causes a Large Increase in APOBEC3A Expression and a Moderate Increase in APOBEC3B Expression in a Normal Human Keratinocyte Cell Line without Increasing Genomic Uracils
Article Snippet: .. One million cells were grown in T25 cell culture flasks for 24 h. Different concentrations of PMA (Sigma) alone or in combination with 10 ng/ml TNF-α (Stem Cell Technologies) were added to the media, and the cells grown further for the indicated lengths of time. ..

Article Title: Long-term Brillouin imaging of live cells with reduced absorption-mediated damage at 660 nm wavelength
Article Snippet: .. They were grown in T25 cell culture flasks in the standard 3T3 medium: Dulbecco’s modified Eagle medium (DMEM) supplemented with 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate (Sigma-Aldrich, catalog no. D5796). .. The DMEM formulation was supplemented with 10% (v/v) bovine calf serum (ATCC 30-2030), and 50 U/mL penicillin and 50 µg/ml streptomycin (Thermo Fisher, catalog no. 15070063).

Article Title: Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny
Article Snippet: .. Karyotyping of the hinny, stallion, and Jack donkey Small pieces of testis parenchyma were cultured in T25 cell culture flasks (Sigma–Aldrich, St Louis, MO, USA) and maintained at 37°C in a humidified 5% CO2 atmosphere. .. The culture medium used was Alpha Minimal Essential Medium (MEM-Apha, GIBCO, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; HyClone, Logan, UT, USA) and 1% penicillin/streptomycin (GIBCO, USA); the medium was changed every 2 to 3 days.

Article Title: Macrophage Immune Response Suppression by Recombinant Mycobacterium tuberculosis Antigens, the ESAT-6, CFP-10, and ESAT-6/CFP-10 Fusion Proteins
Article Snippet: .. In all the experiments, THP-1 (Pasteur Institute, Tehran, Iran) monocytic cells (1×106 cells) were differentiated into macrophages in a T25 Cell Culture Flask containing 3 mL of an RPMI 1640 medium with 5 ng/mL of phorbol 12-myristate 13-acetate (PMA) (Sigma, Dorset, P8139, UK) over 48 hours. .. In vitro Experiments Differentiated THP-1 macrophages have previously been advocated as an in vitro model of human macrophages.

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    Millipore t25 flasks
    T25 Flasks, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore corning biocoat flask t25 pdl canted vented 50cas
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    Millipore tpp tissue culture flasks
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