Structured Review

Fisher Scientific t25 flask
T25 Flask, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flask/product/Fisher Scientific
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
t25 flask - by Bioz Stars, 2020-04
92/100 stars

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Related Articles

Cell Attachment Assay:

Article Title: Aligned fibers direct collective cell migration to engineer closing and nonclosing wound gaps
Article Snippet: Media from a T25 flask containing adherent NIH 3T3 fibroblasts was aspirated, and the adherent cells were rinsed with phosphate-buffered saline (PBS; Fisher Scientific, Pittsburgh, PA) twice. .. After cell attachment, 2 ml of cell culture media with 1% penicillin/streptomycin (HyClone, Logan, UT) was added to the well to facilitate further cell growth.

Ligation:

Article Title: The Structural Basis of Functional Improvement in Response to Human Umbilical Cord Blood Stem Cell Transplantation in Hearts with Post-Infarct LV Remodeling
Article Snippet: The seven rats that underwent MI surgery were randomly allocated into two groups: 1) LAD ligation without any further therapy (MI, n=3); 2) LAD ligation with nh-UCBSC injection (MI+Cell, n=4). .. The isolated cells were cultured in T25 flask (Fisher Scientific, Hampton, NH) in 7 ml Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (Ham) 1:1 (D-MEM/F12; Invitrogen) supplemented with 1xN2 supplement (Invitrogen), 10% fetal bovine serum (FBS; Invitrogen), 10 ng/ml recombinant human basic fibroblast growth factor (b-FGF; R & D Systems), 10 ng/ml recombinant human epidermal growth factor (EGF; R & D Systems), 100 U penicillin (Invitrogen), and 1000 U streptomycin (Invitrogen).

Isolation:

Article Title: The Structural Basis of Functional Improvement in Response to Human Umbilical Cord Blood Stem Cell Transplantation in Hearts with Post-Infarct LV Remodeling
Article Snippet: .. The isolated cells were cultured in T25 flask (Fisher Scientific, Hampton, NH) in 7 ml Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (Ham) 1:1 (D-MEM/F12; Invitrogen) supplemented with 1xN2 supplement (Invitrogen), 10% fetal bovine serum (FBS; Invitrogen), 10 ng/ml recombinant human basic fibroblast growth factor (b-FGF; R & D Systems), 10 ng/ml recombinant human epidermal growth factor (EGF; R & D Systems), 100 U penicillin (Invitrogen), and 1000 U streptomycin (Invitrogen). .. When cells were 70% – 80% confluent, they were detached with 0.25% trypsin-EDTA (Invitrogen) and split at 1:4 for future usage.

Cell Culture:

Article Title: Aligned fibers direct collective cell migration to engineer closing and nonclosing wound gaps
Article Snippet: Paragraph title: Cell culture and seeding ... Media from a T25 flask containing adherent NIH 3T3 fibroblasts was aspirated, and the adherent cells were rinsed with phosphate-buffered saline (PBS; Fisher Scientific, Pittsburgh, PA) twice.

Article Title: The Structural Basis of Functional Improvement in Response to Human Umbilical Cord Blood Stem Cell Transplantation in Hearts with Post-Infarct LV Remodeling
Article Snippet: .. The isolated cells were cultured in T25 flask (Fisher Scientific, Hampton, NH) in 7 ml Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (Ham) 1:1 (D-MEM/F12; Invitrogen) supplemented with 1xN2 supplement (Invitrogen), 10% fetal bovine serum (FBS; Invitrogen), 10 ng/ml recombinant human basic fibroblast growth factor (b-FGF; R & D Systems), 10 ng/ml recombinant human epidermal growth factor (EGF; R & D Systems), 100 U penicillin (Invitrogen), and 1000 U streptomycin (Invitrogen). .. When cells were 70% – 80% confluent, they were detached with 0.25% trypsin-EDTA (Invitrogen) and split at 1:4 for future usage.

Incubation:

Article Title: Aligned fibers direct collective cell migration to engineer closing and nonclosing wound gaps
Article Snippet: Media from a T25 flask containing adherent NIH 3T3 fibroblasts was aspirated, and the adherent cells were rinsed with phosphate-buffered saline (PBS; Fisher Scientific, Pittsburgh, PA) twice. .. They were then incubated with 500 µl 0.25% trypsin (HyClone, Logan, UT) for 5 min at 37°C and suspended in fresh cell culture media.

Modification:

Article Title: The Structural Basis of Functional Improvement in Response to Human Umbilical Cord Blood Stem Cell Transplantation in Hearts with Post-Infarct LV Remodeling
Article Snippet: .. The isolated cells were cultured in T25 flask (Fisher Scientific, Hampton, NH) in 7 ml Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (Ham) 1:1 (D-MEM/F12; Invitrogen) supplemented with 1xN2 supplement (Invitrogen), 10% fetal bovine serum (FBS; Invitrogen), 10 ng/ml recombinant human basic fibroblast growth factor (b-FGF; R & D Systems), 10 ng/ml recombinant human epidermal growth factor (EGF; R & D Systems), 100 U penicillin (Invitrogen), and 1000 U streptomycin (Invitrogen). .. When cells were 70% – 80% confluent, they were detached with 0.25% trypsin-EDTA (Invitrogen) and split at 1:4 for future usage.

Injection:

Article Title: The Structural Basis of Functional Improvement in Response to Human Umbilical Cord Blood Stem Cell Transplantation in Hearts with Post-Infarct LV Remodeling
Article Snippet: The seven rats that underwent MI surgery were randomly allocated into two groups: 1) LAD ligation without any further therapy (MI, n=3); 2) LAD ligation with nh-UCBSC injection (MI+Cell, n=4). .. The isolated cells were cultured in T25 flask (Fisher Scientific, Hampton, NH) in 7 ml Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (Ham) 1:1 (D-MEM/F12; Invitrogen) supplemented with 1xN2 supplement (Invitrogen), 10% fetal bovine serum (FBS; Invitrogen), 10 ng/ml recombinant human basic fibroblast growth factor (b-FGF; R & D Systems), 10 ng/ml recombinant human epidermal growth factor (EGF; R & D Systems), 100 U penicillin (Invitrogen), and 1000 U streptomycin (Invitrogen).

Recombinant:

Article Title: The Structural Basis of Functional Improvement in Response to Human Umbilical Cord Blood Stem Cell Transplantation in Hearts with Post-Infarct LV Remodeling
Article Snippet: .. The isolated cells were cultured in T25 flask (Fisher Scientific, Hampton, NH) in 7 ml Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (Ham) 1:1 (D-MEM/F12; Invitrogen) supplemented with 1xN2 supplement (Invitrogen), 10% fetal bovine serum (FBS; Invitrogen), 10 ng/ml recombinant human basic fibroblast growth factor (b-FGF; R & D Systems), 10 ng/ml recombinant human epidermal growth factor (EGF; R & D Systems), 100 U penicillin (Invitrogen), and 1000 U streptomycin (Invitrogen). .. When cells were 70% – 80% confluent, they were detached with 0.25% trypsin-EDTA (Invitrogen) and split at 1:4 for future usage.

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    Fisher Scientific t25 flasks
    T25 Flasks, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Fisher Scientific
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    92
    Fisher Scientific t25 non tissue culture treated flasks
    T25 Non Tissue Culture Treated Flasks, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 non tissue culture treated flasks/product/Fisher Scientific
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t25 non tissue culture treated flasks - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    92
    Fisher Scientific t25 flask
    T25 Flask, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Fisher Scientific
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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