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Corning Life Sciences t25 flask
T25 Flask, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flask/product/Corning Life Sciences
Average 95 stars, based on 6 article reviews
Price from $9.99 to $1999.99
t25 flask - by Bioz Stars, 2020-04
95/100 stars

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Transduction:

Article Title: The ULb? Region of the Human Cytomegalovirus Genome Confers an Increased Requirement for the Viral Protein Kinase UL97
Article Snippet: .. To transduce HFF, 4 × 105 HFF were seeded in a T25 flask (Corning, Inc.) and allowed to attach overnight. .. On the following day, 0.5 to 1 ml of lentivirus supernatant was applied in a final volume of 3 ml complete medium supplemented with 8 μg/ml hexadimethrine bromide (Polybrene; Santa Cruz Biotechnology, Santa Cruz, CA).

Article Title: 3K3A-APC stimulates post-ischemic neuronal repair by human neural stem cells in mice
Article Snippet: .. For transduction, 0.5 × 106 single cells in 300 μl of DMEM-F12 medium were inoculated with one viral stock ( > 2×109 IFUs) for 1 hour at 37 °C, 5% CO2 in a humidified atmosphere with agitation every 10–15 minutes, before transferred to T25 flask (Corning). .. Transduced neurospheres were collected after 3–4 d and dissociated with StemPro Accutase for transplantation.

Produced:

Article Title: The ULb? Region of the Human Cytomegalovirus Genome Confers an Increased Requirement for the Viral Protein Kinase UL97
Article Snippet: Lentivirus vector particles were produced and used to transduce HFF by following the Addgene pLKO.1 protocol ( ). .. To transduce HFF, 4 × 105 HFF were seeded in a T25 flask (Corning, Inc.) and allowed to attach overnight.

Transfection:

Article Title: The ULb? Region of the Human Cytomegalovirus Genome Confers an Increased Requirement for the Viral Protein Kinase UL97
Article Snippet: Transfection reagents were removed after overnight incubation and replaced with complete medium. .. To transduce HFF, 4 × 105 HFF were seeded in a T25 flask (Corning, Inc.) and allowed to attach overnight.

Article Title: Endothelial Cells Can Regulate Smooth Muscle Cells in Contractile Phenotype through the miR-206/ARF6 NCX1/Exosome Axis
Article Snippet: .. Briefly, HUVECs were grown to 60% confluence in a T25 flask (Corning Life Science, Tewksbury, MA, USA) and transfected with 200 pmol of siRNA or miRNA. ..

Article Title: A split horseradish peroxidase for detection of intercellular protein-protein interactions and sensitive visualization of synapses
Article Snippet: .. Production of lentiviruses HEK 293T cells in a T25 flask (Corning) were transfected at ~60–70% confluency with 3 mL of serum-free media containing the lentiviral vector containing the gene of interest (2.5 μg), the lentiviral packaging plasmids dR8.91 (2.25 μg) and pVSV-G (0.25 μg) , and 30 μL of 1 mg/mL Polyethylenimine “Max” (Polysciences) pH 7.1 for 3 h. Transfection media was replaced with fresh serum-containing media, and cell media containing lentivirus was harvested after 48 h and passed through a 0.45 μm filter to produce infectious media that was added directly to neuronal cultures, as described below. .. Production of adeno-associated viruses Adeno-associated viruses (AAVs) with a mixed serotype AAV1/AAV2 were produced according to a published procedure , except that the HEK293T cells were transfected differently.

In Vitro:

Article Title: Embryonic stem cell preconditioned microenvironment suppresses tumorigenic properties in breast cancer
Article Snippet: The medium for 4T1 cells was changed to 7 ml RPMI 1640 and 5 % FBS per T25 flask (Corning). .. As already described, ES cells can maintain the self-renewal and pluripotency properties in vitro, but need fibroblast feeder cells for support.

Isolation:

Article Title: Utilizing the nanosecond pulse technique to improve antigen intracellular delivery and presentation to treat tongue squamous cell carcinoma
Article Snippet: - Isolation and induction of mature DCs Freshly isolated PBMCs were resuspended in RPMI complete medium, and the cell density was adjusted to 4 × 106/mL (5 mL). .. Then, the cells were cultured in a T25 flask (Corning, Corning, New York, USA) (37 °C, 4 h, 5% CO2) in an incubator, and the culture supernatant and non-adherent cells were discarded by sterile straw aspiration.

Article Title: Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
Article Snippet: Paragraph title: Isolation and culture of cells ... The cells were suspended in a T25 flask (Corning, New York, USA) containing DMEM/F12 supplemented with 10% foetal bovine serum (FBS) (SiJiqing, China), streptomycin (100 mg/ml), and penicillin (100 U/ml) and then cultured in a humidified incubator at 37 °C in 5% CO2 .

Article Title: Endothelial Cells Can Regulate Smooth Muscle Cells in Contractile Phenotype through the miR-206/ARF6 NCX1/Exosome Axis
Article Snippet: Paragraph title: Exosome isolation ... Briefly, HUVECs were grown to 60% confluence in a T25 flask (Corning Life Science, Tewksbury, MA, USA) and transfected with 200 pmol of siRNA or miRNA.

Article Title: Development of a Novel CD4+ TCR Transgenic Line That Reveals a Dominant Role for CD8+ Dendritic Cells and CD40 Signaling in the Generation of Helper and CTL Responses to Blood-Stage Malaria
Article Snippet: Synchronous schizont-stage parasites were isolated from uninfected erythrocytes by passage through a magnetized column (Miltenyi Biotec) in PBS. .. Freshly egressed tachyzoites from a T25 flask (Corning) were passed through a 27-gauge needle to ensure complete release from HFFs, before centrifuging at 450 rpm for 5 min at room temperature to pellet host cell debris.

Transferring:

Article Title: Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip
Article Snippet: .. T25 flask with canted vented cap (Corning Inc., cat. no. 353108) Filter system 0.22 μm PES 500mL (Corning Inc., cat. no. 431097) Steriflip 0.22 μm PES (EMD Millipore, cat. no. SCGP00525) Tissue culture treated 6-well plates (Corning Inc., cat. no. 353046) Tissue culture treated 12-well plates (Corning Inc., cat. no. 353043) Falcom 150 mm cell culture dish (Corning Inc., cat. No. 353025) Wide beveled cell lifter (Corning Inc., cat. no. 3008) Cryogenic vial rack and tray (Corning Inc., cat. no. 430525) Conical centrifuge tube, 15 mL (Corning Inc., cat. no. 352097) Conical centrifuge tube, 50 mL (Corning Inc., cat. no. 352098) CryoTube vials (NUNC Inc., cat. no. 377267) Aspirating pipettes, individually wrapped (Corning Inc., cat. no. 29442–462) P10 precision barrier pipette tips (Denville Scientific, cat. no. P1096-FR) P20 barrier pipette tips (Denville Scientific, cat. no. P1121) P100 barrier pipette tips (Denville Scientific, cat. no. P1125) P200 barrier pipette tips (Denville Scientific, cat. no. P1122) P1000 barrier pipette tips (Denville Scientific, cat. no. P1126) Serological pipette, 5 mL, individually wrapped (Corning Inc., cat. no. 356543) Serological pipette, 10 mL, individually wrapped (Corning Inc., cat. no. 356551) Serological pipette, 25 mL, individually wrapped (Corning Inc., cat. no. 356525) Stainless steel disposable safety scalpels (Integra Miltrex, cat. no. 4–510) Kim wipes, small (VWR, cat. no. 21905–026) Kim wipes, large (VWR, cat. no. 21905–049) Kimberly-Clark nitrile gloves, long cuff (VWR, cat. no. 50601) Kimber-Clark basic plus lab coat (VWR, cat. no. 37000–922) Tyvek isoclean sleeves (VWR, cat. no. 89127–350) Ethanol solution, 70% (v/v), biotechnology grade (VWR, cat. no. 97065–058) ! ..

In Vivo Imaging:

Article Title: 3K3A-APC stimulates post-ischemic neuronal repair by human neural stem cells in mice
Article Snippet: Paragraph title: In vivo imaging of transplanted cells ... For transduction, 0.5 × 106 single cells in 300 μl of DMEM-F12 medium were inoculated with one viral stock ( > 2×109 IFUs) for 1 hour at 37 °C, 5% CO2 in a humidified atmosphere with agitation every 10–15 minutes, before transferred to T25 flask (Corning).

Centrifugation:

Article Title: Endothelial Cells Can Regulate Smooth Muscle Cells in Contractile Phenotype through the miR-206/ARF6 NCX1/Exosome Axis
Article Snippet: Exosome isolation Exosomes were isolated by a multi-step centrifugation process as described previously [ ]. .. Briefly, HUVECs were grown to 60% confluence in a T25 flask (Corning Life Science, Tewksbury, MA, USA) and transfected with 200 pmol of siRNA or miRNA.

Spectrophotometry:

Article Title: Shigella pathogenesis modeling with tissue culture assays
Article Snippet: .. Shigella glycerol freezer stock Tryptic soy agar plates with 0.01% w/v congo red (TSA + CR) 37 °C incubator Luria-Bertani broth (LB) Sodium deoxycholate MEM (see recipe) Henle-407 (ATCC CCL6) cultured in a T25 flask 37 °C humidified incubator with 5% CO2 6-well tissue-culture treated plate (Corning #3516) Trypsin 0.05% final concentration Spectrophotometer Centrifuge capable of spinning plates Phosphate buffered saline (PBS) Gentamicin sulfate 20 μg / mL final concentration Automated cell counter or hemocytometer ..

Mouse Assay:

Article Title: Development of a Novel CD4+ TCR Transgenic Line That Reveals a Dominant Role for CD8+ Dendritic Cells and CD40 Signaling in the Generation of Helper and CTL Responses to Blood-Stage Malaria
Article Snippet: Paragraph title: Mice, mosquitos, and parasites ... Freshly egressed tachyzoites from a T25 flask (Corning) were passed through a 27-gauge needle to ensure complete release from HFFs, before centrifuging at 450 rpm for 5 min at room temperature to pellet host cell debris.

Light Microscopy:

Article Title: Impaired Function of Bone Marrow Stromal Cells in Systemic Mastocytosis
Article Snippet: Briefly, 1× 105 nucleated cells were plated in T25 flask (Corning, Corning, NY) in duplicate and cultured for 13 days without changing the culture medium. .. Colonies were observed under low magnitude light microscope field (25 X).

Concentration Assay:

Article Title: Utilizing the nanosecond pulse technique to improve antigen intracellular delivery and presentation to treat tongue squamous cell carcinoma
Article Snippet: The cells were suspended in RPMI 1640 (Gibco, Grand Island, New York, USA) + 10% FBS (Gibco, Grand Island, New York, USA) complete medium, and the final concentration of the PBMCs was 2 × 105/mL. .. Then, the cells were cultured in a T25 flask (Corning, Corning, New York, USA) (37 °C, 4 h, 5% CO2) in an incubator, and the culture supernatant and non-adherent cells were discarded by sterile straw aspiration.

Article Title: Shigella pathogenesis modeling with tissue culture assays
Article Snippet: .. Shigella glycerol freezer stock Tryptic soy agar plates with 0.01% w/v congo red (TSA + CR) 37 °C incubator Luria-Bertani broth (LB) Sodium deoxycholate MEM (see recipe) Henle-407 (ATCC CCL6) cultured in a T25 flask 37 °C humidified incubator with 5% CO2 6-well tissue-culture treated plate (Corning #3516) Trypsin 0.05% final concentration Spectrophotometer Centrifuge capable of spinning plates Phosphate buffered saline (PBS) Gentamicin sulfate 20 μg / mL final concentration Automated cell counter or hemocytometer ..

Incubation:

Article Title: The ULb? Region of the Human Cytomegalovirus Genome Confers an Increased Requirement for the Viral Protein Kinase UL97
Article Snippet: Transfection reagents were removed after overnight incubation and replaced with complete medium. .. To transduce HFF, 4 × 105 HFF were seeded in a T25 flask (Corning, Inc.) and allowed to attach overnight.

other:

Article Title: Increasing radiosensitivity with the downregulation of cofilin-1 in U251 human glioma cells
Article Snippet: Establishment of radioresistant U251 cells (RR-U251) U251 cells were seeded at a density of 1×105 in a T25 flask (Corning Inc., Corning, NY, USA) in complete medium.

Cell Culture:

Article Title: Utilizing the nanosecond pulse technique to improve antigen intracellular delivery and presentation to treat tongue squamous cell carcinoma
Article Snippet: .. Then, the cells were cultured in a T25 flask (Corning, Corning, New York, USA) (37 °C, 4 h, 5% CO2) in an incubator, and the culture supernatant and non-adherent cells were discarded by sterile straw aspiration. ..

Article Title: Shigella pathogenesis modeling with tissue culture assays
Article Snippet: .. Shigella glycerol freezer stock Tryptic soy agar plates with 0.01% w/v congo red (TSA + CR) 37 °C incubator Luria-Bertani broth (LB) Sodium deoxycholate MEM (see recipe) Henle-407 (ATCC CCL6) cultured in a T25 flask 37 °C humidified incubator with 5% CO2 6-well tissue-culture treated plate (Corning #3516) Trypsin 0.05% final concentration Spectrophotometer Centrifuge capable of spinning plates Phosphate buffered saline (PBS) Gentamicin sulfate 20 μg / mL final concentration Automated cell counter or hemocytometer ..

Article Title: Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip
Article Snippet: .. T25 flask with canted vented cap (Corning Inc., cat. no. 353108) Filter system 0.22 μm PES 500mL (Corning Inc., cat. no. 431097) Steriflip 0.22 μm PES (EMD Millipore, cat. no. SCGP00525) Tissue culture treated 6-well plates (Corning Inc., cat. no. 353046) Tissue culture treated 12-well plates (Corning Inc., cat. no. 353043) Falcom 150 mm cell culture dish (Corning Inc., cat. No. 353025) Wide beveled cell lifter (Corning Inc., cat. no. 3008) Cryogenic vial rack and tray (Corning Inc., cat. no. 430525) Conical centrifuge tube, 15 mL (Corning Inc., cat. no. 352097) Conical centrifuge tube, 50 mL (Corning Inc., cat. no. 352098) CryoTube vials (NUNC Inc., cat. no. 377267) Aspirating pipettes, individually wrapped (Corning Inc., cat. no. 29442–462) P10 precision barrier pipette tips (Denville Scientific, cat. no. P1096-FR) P20 barrier pipette tips (Denville Scientific, cat. no. P1121) P100 barrier pipette tips (Denville Scientific, cat. no. P1125) P200 barrier pipette tips (Denville Scientific, cat. no. P1122) P1000 barrier pipette tips (Denville Scientific, cat. no. P1126) Serological pipette, 5 mL, individually wrapped (Corning Inc., cat. no. 356543) Serological pipette, 10 mL, individually wrapped (Corning Inc., cat. no. 356551) Serological pipette, 25 mL, individually wrapped (Corning Inc., cat. no. 356525) Stainless steel disposable safety scalpels (Integra Miltrex, cat. no. 4–510) Kim wipes, small (VWR, cat. no. 21905–026) Kim wipes, large (VWR, cat. no. 21905–049) Kimberly-Clark nitrile gloves, long cuff (VWR, cat. no. 50601) Kimber-Clark basic plus lab coat (VWR, cat. no. 37000–922) Tyvek isoclean sleeves (VWR, cat. no. 89127–350) Ethanol solution, 70% (v/v), biotechnology grade (VWR, cat. no. 97065–058) ! ..

Article Title: Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
Article Snippet: .. The cells were suspended in a T25 flask (Corning, New York, USA) containing DMEM/F12 supplemented with 10% foetal bovine serum (FBS) (SiJiqing, China), streptomycin (100 mg/ml), and penicillin (100 U/ml) and then cultured in a humidified incubator at 37 °C in 5% CO2 . ..

Article Title: Embryonic stem cell preconditioned microenvironment suppresses tumorigenic properties in breast cancer
Article Snippet: Collection of conditioned medium The mES cells, 4T1 cells, or SNL cells (purchased from ATCC) were cultured to 40 % confluence with each usual culture medium respectively, and the medium for ES cells and SNL cells was changed to 7 ml DMEM and 5 % FBS per T25 flask (Corning). .. The medium for 4T1 cells was changed to 7 ml RPMI 1640 and 5 % FBS per T25 flask (Corning).

Article Title: Impaired Function of Bone Marrow Stromal Cells in Systemic Mastocytosis
Article Snippet: .. Briefly, 1× 105 nucleated cells were plated in T25 flask (Corning, Corning, NY) in duplicate and cultured for 13 days without changing the culture medium. .. The colonies were fixed with methanol for 30 minutes and stained with saturated methyl-violet water solution for 20 minutes.

Article Title: Development of a Novel CD4+ TCR Transgenic Line That Reveals a Dominant Role for CD8+ Dendritic Cells and CD40 Signaling in the Generation of Helper and CTL Responses to Blood-Stage Malaria
Article Snippet: The P. falciparum 3D7 strain was cultured in human erythrocytes (Australian Red Cross Blood Bank, blood group O+ ) at 4% hematocrit in AlbuMAX II media (RPMI 1640–HEPES, 0.5% AlbuMAX II [Life Technologies], 0.2% NaHCO3 ) at 37°C as described previously ( ). .. Freshly egressed tachyzoites from a T25 flask (Corning) were passed through a 27-gauge needle to ensure complete release from HFFs, before centrifuging at 450 rpm for 5 min at room temperature to pellet host cell debris.

Transplantation Assay:

Article Title: 3K3A-APC stimulates post-ischemic neuronal repair by human neural stem cells in mice
Article Snippet: For transduction, 0.5 × 106 single cells in 300 μl of DMEM-F12 medium were inoculated with one viral stock ( > 2×109 IFUs) for 1 hour at 37 °C, 5% CO2 in a humidified atmosphere with agitation every 10–15 minutes, before transferred to T25 flask (Corning). .. Transduced neurospheres were collected after 3–4 d and dissociated with StemPro Accutase for transplantation.

Staining:

Article Title: Impaired Function of Bone Marrow Stromal Cells in Systemic Mastocytosis
Article Snippet: Briefly, 1× 105 nucleated cells were plated in T25 flask (Corning, Corning, NY) in duplicate and cultured for 13 days without changing the culture medium. .. The colonies were fixed with methanol for 30 minutes and stained with saturated methyl-violet water solution for 20 minutes.

Infection:

Article Title: Development of a Novel CD4+ TCR Transgenic Line That Reveals a Dominant Role for CD8+ Dendritic Cells and CD40 Signaling in the Generation of Helper and CTL Responses to Blood-Stage Malaria
Article Snippet: Unless otherwise stated, mice were infected i.v. with 104 PbA, P. berghei NK65, P. chabaudi , or P. yoelii 17XNL infected RBC (iRBC) in 0.2 ml of PBS. .. Freshly egressed tachyzoites from a T25 flask (Corning) were passed through a 27-gauge needle to ensure complete release from HFFs, before centrifuging at 450 rpm for 5 min at room temperature to pellet host cell debris.

Plasmid Preparation:

Article Title: The ULb? Region of the Human Cytomegalovirus Genome Confers an Increased Requirement for the Viral Protein Kinase UL97
Article Snippet: For each lentivirus vector, 24-h and 48-h supernatants were collected, pooled, spun for 5 min at 1,250 rpm, filtered through a 0.45-μm-pore-size syringe filter (Corning, Inc.), and frozen at −80°C until use. .. To transduce HFF, 4 × 105 HFF were seeded in a T25 flask (Corning, Inc.) and allowed to attach overnight.

Article Title: A split horseradish peroxidase for detection of intercellular protein-protein interactions and sensitive visualization of synapses
Article Snippet: .. Production of lentiviruses HEK 293T cells in a T25 flask (Corning) were transfected at ~60–70% confluency with 3 mL of serum-free media containing the lentiviral vector containing the gene of interest (2.5 μg), the lentiviral packaging plasmids dR8.91 (2.25 μg) and pVSV-G (0.25 μg) , and 30 μL of 1 mg/mL Polyethylenimine “Max” (Polysciences) pH 7.1 for 3 h. Transfection media was replaced with fresh serum-containing media, and cell media containing lentivirus was harvested after 48 h and passed through a 0.45 μm filter to produce infectious media that was added directly to neuronal cultures, as described below. .. Production of adeno-associated viruses Adeno-associated viruses (AAVs) with a mixed serotype AAV1/AAV2 were produced according to a published procedure , except that the HEK293T cells were transfected differently.

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  • 94
    Corning Life Sciences t25 flasks
    T25 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Corning Life Sciences
    Average 94 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    93
    Corning Life Sciences plastic closed cap t25 flasks
    Plastic Closed Cap T25 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plastic closed cap t25 flasks/product/Corning Life Sciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plastic closed cap t25 flasks - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    92
    Corning Life Sciences confluent t25 flask
    Confluent T25 Flask, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/confluent t25 flask/product/Corning Life Sciences
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    confluent t25 flask - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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