Structured Review

Corning Life Sciences t25 flask
T25 Flask, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flask/product/Corning Life Sciences
Average 92 stars, based on 18 article reviews
Price from $9.99 to $1999.99
t25 flask - by Bioz Stars, 2020-07
92/100 stars

Images

Related Articles

Transduction:

Article Title: 3K3A-APC stimulates post-ischemic neuronal repair by human neural stem cells in mice
Article Snippet: .. For transduction, 0.5 × 106 single cells in 300 μl of DMEM-F12 medium were inoculated with one viral stock ( > 2×109 IFUs) for 1 hour at 37 °C, 5% CO2 in a humidified atmosphere with agitation every 10–15 minutes, before transferred to T25 flask (Corning). .. Transduced neurospheres were collected after 3–4 d and dissociated with StemPro Accutase for transplantation.

Transfection:

Article Title: Endothelial Cells Can Regulate Smooth Muscle Cells in Contractile Phenotype through the miR-206/ARF6 NCX1/Exosome Axis
Article Snippet: .. Briefly, HUVECs were grown to 60% confluence in a T25 flask (Corning Life Science, Tewksbury, MA, USA) and transfected with 200 pmol of siRNA or miRNA. ..

Article Title: A split horseradish peroxidase for detection of intercellular protein-protein interactions and sensitive visualization of synapses
Article Snippet: .. Production of lentiviruses HEK 293T cells in a T25 flask (Corning) were transfected at ~60–70% confluency with 3 mL of serum-free media containing the lentiviral vector containing the gene of interest (2.5 μg), the lentiviral packaging plasmids dR8.91 (2.25 μg) and pVSV-G (0.25 μg) , and 30 μL of 1 mg/mL Polyethylenimine “Max” (Polysciences) pH 7.1 for 3 h. Transfection media was replaced with fresh serum-containing media, and cell media containing lentivirus was harvested after 48 h and passed through a 0.45 μm filter to produce infectious media that was added directly to neuronal cultures, as described below. .. Production of adeno-associated viruses Adeno-associated viruses (AAVs) with a mixed serotype AAV1/AAV2 were produced according to a published procedure , except that the HEK293T cells were transfected differently.

Cell Culture:

Article Title: Utilizing the nanosecond pulse technique to improve antigen intracellular delivery and presentation to treat tongue squamous cell carcinoma
Article Snippet: .. Then, the cells were cultured in a T25 flask (Corning, Corning, New York, USA) (37 °C, 4 h, 5% CO2) in an incubator, and the culture supernatant and non-adherent cells were discarded by sterile straw aspiration. ..

Article Title: Shigella pathogenesis modeling with tissue culture assays
Article Snippet: .. Shigella glycerol freezer stock Tryptic soy agar plates with 0.01% w/v congo red (TSA + CR) 37 °C incubator Luria-Bertani broth (LB) Sodium deoxycholate MEM (see recipe) Henle-407 (ATCC CCL6) cultured in a T25 flask 37 °C humidified incubator with 5% CO2 6-well tissue-culture treated plate (Corning #3516) Trypsin 0.05% final concentration Spectrophotometer Centrifuge capable of spinning plates Phosphate buffered saline (PBS) Gentamicin sulfate 20 μg / mL final concentration Automated cell counter or hemocytometer ..

Article Title: Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
Article Snippet: .. The cells were suspended in a T25 flask (Corning, New York, USA) containing DMEM/F12 supplemented with 10% foetal bovine serum (FBS) (SiJiqing, China), streptomycin (100 mg/ml), and penicillin (100 U/ml) and then cultured in a humidified incubator at 37 °C in 5% CO2 . ..

Article Title: Impaired Function of Bone Marrow Stromal Cells in Systemic Mastocytosis
Article Snippet: .. Briefly, 1× 105 nucleated cells were plated in T25 flask (Corning, Corning, NY) in duplicate and cultured for 13 days without changing the culture medium. .. The colonies were fixed with methanol for 30 minutes and stained with saturated methyl-violet water solution for 20 minutes.

Concentration Assay:

Article Title: Shigella pathogenesis modeling with tissue culture assays
Article Snippet: .. Shigella glycerol freezer stock Tryptic soy agar plates with 0.01% w/v congo red (TSA + CR) 37 °C incubator Luria-Bertani broth (LB) Sodium deoxycholate MEM (see recipe) Henle-407 (ATCC CCL6) cultured in a T25 flask 37 °C humidified incubator with 5% CO2 6-well tissue-culture treated plate (Corning #3516) Trypsin 0.05% final concentration Spectrophotometer Centrifuge capable of spinning plates Phosphate buffered saline (PBS) Gentamicin sulfate 20 μg / mL final concentration Automated cell counter or hemocytometer ..

Spectrophotometry:

Article Title: Shigella pathogenesis modeling with tissue culture assays
Article Snippet: .. Shigella glycerol freezer stock Tryptic soy agar plates with 0.01% w/v congo red (TSA + CR) 37 °C incubator Luria-Bertani broth (LB) Sodium deoxycholate MEM (see recipe) Henle-407 (ATCC CCL6) cultured in a T25 flask 37 °C humidified incubator with 5% CO2 6-well tissue-culture treated plate (Corning #3516) Trypsin 0.05% final concentration Spectrophotometer Centrifuge capable of spinning plates Phosphate buffered saline (PBS) Gentamicin sulfate 20 μg / mL final concentration Automated cell counter or hemocytometer ..

Plasmid Preparation:

Article Title: A split horseradish peroxidase for detection of intercellular protein-protein interactions and sensitive visualization of synapses
Article Snippet: .. Production of lentiviruses HEK 293T cells in a T25 flask (Corning) were transfected at ~60–70% confluency with 3 mL of serum-free media containing the lentiviral vector containing the gene of interest (2.5 μg), the lentiviral packaging plasmids dR8.91 (2.25 μg) and pVSV-G (0.25 μg) , and 30 μL of 1 mg/mL Polyethylenimine “Max” (Polysciences) pH 7.1 for 3 h. Transfection media was replaced with fresh serum-containing media, and cell media containing lentivirus was harvested after 48 h and passed through a 0.45 μm filter to produce infectious media that was added directly to neuronal cultures, as described below. .. Production of adeno-associated viruses Adeno-associated viruses (AAVs) with a mixed serotype AAV1/AAV2 were produced according to a published procedure , except that the HEK293T cells were transfected differently.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Corning Life Sciences t25 flasks
    T25 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Corning Life Sciences
    Average 92 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Corning Life Sciences plastic closed cap t25 flasks
    Plastic Closed Cap T25 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plastic closed cap t25 flasks/product/Corning Life Sciences
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plastic closed cap t25 flasks - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    Corning Life Sciences confluent t25 flask
    Confluent T25 Flask, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/confluent t25 flask/product/Corning Life Sciences
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    confluent t25 flask - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results