Structured Review

Avantor t25 flask
T25 Flask, supplied by Avantor, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flask/product/Avantor
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t25 flask - by Bioz Stars, 2020-04
94/100 stars

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Related Articles

Cell Isolation:

Article Title: Functional Enrichment and Analysis of Antigen-Specific Memory B Cell Antibody Repertoires in PBMCs
Article Snippet: The sample was resuspended in growth media and B cells were negatively selected using the EasySep Human B cell Isolation Kit according to the manufacturer's protocol (STEMCELL Technologies, Vancouver, BC). .. The day before PBMCs were thawed, 1.2 × 106 feeder cells were seeded in a T25 flask (VWR) in 4 ml of B cell growth media and cultured overnight in a humidified 37°C, 5% CO2 incubator.

Cell Culture:

Article Title: Functional Enrichment and Analysis of Antigen-Specific Memory B Cell Antibody Repertoires in PBMCs
Article Snippet: .. The day before PBMCs were thawed, 1.2 × 106 feeder cells were seeded in a T25 flask (VWR) in 4 ml of B cell growth media and cultured overnight in a humidified 37°C, 5% CO2 incubator. .. PBMCs were quickly thawed at 37°C, washed 1x in 10 ml of growth media, and resuspended in 4 ml of 2x B cell growth media containing 2 × ITS from 100x Insulin, Transferrin, Selenium (Thermo Fisher Scientific), 20 ng/ml IL-10, 2 ng/ml IL-2, 10 ng/ml IL-15, 10 ng/ml IL-6 (R & D Systems, Minneapolis, MN) and 4 μg/ml CpG (ODN 2006-G5, InvivoGen, San Diego, CA).

Purification:

Article Title: Functional Enrichment and Analysis of Antigen-Specific Memory B Cell Antibody Repertoires in PBMCs
Article Snippet: The final cells from negative enrichment were pelleted at 350 × g for 5 min, resuspended and lysed in Qiagen RLT buffer with beta-mercaptoethanol for 10 min, frozen on dry ice, and transferred to −80°C storage until RNA purification with the Qiagen AllPrep RNA/DNA kit (Qiagen, Hilden, Germany). .. The day before PBMCs were thawed, 1.2 × 106 feeder cells were seeded in a T25 flask (VWR) in 4 ml of B cell growth media and cultured overnight in a humidified 37°C, 5% CO2 incubator.