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tetrodotoxin citrate  (Alomone Labs)


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    Alomone Labs tetrodotoxin citrate
    Tetrodotoxin Citrate, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t-550/custom%40t-550%4010%2E64898%2F2026%2E05%2E01%2E719272?v=Alomone+Labs
    Average 95 stars, based on 205 article reviews
    tetrodotoxin citrate - by Bioz Stars, 2026-07
    95/100 stars

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    Determination of the limit of detection (LOD) and lower limit of quantification (LLOQ) for the two-step method. CAR-T cell absolute counts were measured in 31 negative control samples from patients not treated with CD19 CAR-T cells. The mean background signal is represented by the blue line. LOD was defined as mean + 3 standard deviations (SD), and LLOQ as mean + 10 SD.

    Journal: Frontiers in Oncology

    Article Title: Clinical implementation of a one-step no-wash flow cytometry method allows for real-time monitoring of patients treated with autologous CAR-T cells

    doi: 10.3389/fonc.2026.1774431

    Figure Lengend Snippet: Determination of the limit of detection (LOD) and lower limit of quantification (LLOQ) for the two-step method. CAR-T cell absolute counts were measured in 31 negative control samples from patients not treated with CD19 CAR-T cells. The mean background signal is represented by the blue line. LOD was defined as mean + 3 standard deviations (SD), and LLOQ as mean + 10 SD.

    Article Snippet: 100 μL of blood sample were stained with 5μL of CD19 CAR-T detection reagent (130-129–550 Miltenyi Biotec, Bergisch Gladbach, Germany) during 10 minutes at Room Temperature (RT) in the dark.

    Techniques: Negative Control

    Gating strategy for the single-step method. Absolute counting beads were excluded based on scatter and fluorescence properties. Dead cells were excluded using 7-aminoactinomycin D (7-AAD). CD45-positive leukocytes were selected, and lymphocytes were identified according to side scatter (SSC) characteristics. CD3-positive T cells were gated, and CAR-T cells were defined as viable CD45+/CD3+/CAR+ events using directly fluorochrome-conjugated CAR detection reagents (CD19 or BCMA). CD4 and CD8 subpopulations were subsequently identified within the CAR-positive T-cell compartment. Absolute quantification was calculated using TruCount beads according to the manufacturer’s formula.

    Journal: Frontiers in Oncology

    Article Title: Clinical implementation of a one-step no-wash flow cytometry method allows for real-time monitoring of patients treated with autologous CAR-T cells

    doi: 10.3389/fonc.2026.1774431

    Figure Lengend Snippet: Gating strategy for the single-step method. Absolute counting beads were excluded based on scatter and fluorescence properties. Dead cells were excluded using 7-aminoactinomycin D (7-AAD). CD45-positive leukocytes were selected, and lymphocytes were identified according to side scatter (SSC) characteristics. CD3-positive T cells were gated, and CAR-T cells were defined as viable CD45+/CD3+/CAR+ events using directly fluorochrome-conjugated CAR detection reagents (CD19 or BCMA). CD4 and CD8 subpopulations were subsequently identified within the CAR-positive T-cell compartment. Absolute quantification was calculated using TruCount beads according to the manufacturer’s formula.

    Article Snippet: 100 μL of blood sample were stained with 5μL of CD19 CAR-T detection reagent (130-129–550 Miltenyi Biotec, Bergisch Gladbach, Germany) during 10 minutes at Room Temperature (RT) in the dark.

    Techniques: Fluorescence, Quantitative Proteomics

    Determination of LOD and LLOQ for the single-step method. CAR-T cell absolute counts were measured in 10 negative control samples from patients not treated with CD19 or BCMA CAR-T cells. The blue line represents the mean background signal. LOD was defined as mean + 3 SD and LLOQ (green dashed line) as mean + 10 SD.

    Journal: Frontiers in Oncology

    Article Title: Clinical implementation of a one-step no-wash flow cytometry method allows for real-time monitoring of patients treated with autologous CAR-T cells

    doi: 10.3389/fonc.2026.1774431

    Figure Lengend Snippet: Determination of LOD and LLOQ for the single-step method. CAR-T cell absolute counts were measured in 10 negative control samples from patients not treated with CD19 or BCMA CAR-T cells. The blue line represents the mean background signal. LOD was defined as mean + 3 SD and LLOQ (green dashed line) as mean + 10 SD.

    Article Snippet: 100 μL of blood sample were stained with 5μL of CD19 CAR-T detection reagent (130-129–550 Miltenyi Biotec, Bergisch Gladbach, Germany) during 10 minutes at Room Temperature (RT) in the dark.

    Techniques: Negative Control