Journal: bioRxiv
Article Title: Pre-Sensory Spontaneous Activity Accelerates Coordinated Maturation of Synaptic Partners and Drives Transition to the Mature Physiological Phenotype
doi: 10.1101/2025.08.28.672917
Figure Lengend Snippet: TeNT reduces frequency and slows kinetics of spontaneous (s)EPSC in MNTB PNs. (A), Exemplary voltage-clamp traces of P6 PN sEPSCs from non-injected control mouse (grey traces) and from the iMNTB (non-transduced control, black traces) and cMNTB (transduced, red traces) of unilaterally injected animals. Cells clamped at holding potential of −73 mV. Recordings were made after bath application of Gabazine (GABA A receptor antagonist, 10 μM) and strychnine (glycine receptor antagonist, 2 μM). Averaged sEPSC waveforms from each cell shown at bottom with expanded time scale and color-coordinated. (B-E), Plots show reduced frequency, increased average decay time constant (τ) and rise time, and near constant sEPSC amplitude in cMNTB PNs across age. Non-injected control recordings from MNTB PNs (grey circles; P4, n = 10; P6, n =15; P9, n = 13; P14, n = 12) were compared to PNs in the iMNTB (black circles, Control; P4, n = 20; P6, n =20; P9, n = 19; P14, n = 15) and cMNTB (red circles, TeNT; P4, n = 21; P6, n =21; P9, n = 17; P14, n = 11) following unilateral viral injection. Mean (horizontal gray lines) and standard deviation (error bars). Significance levels are indicated according to the convention: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and not significant (ns). Statistical results for panels (B-E) are reported in Table S6.
Article Snippet: For all recordings the glycine receptor antagonist Strychnine (2 μM) (European Pharmacopoeia) and GABA A receptor antagonist SR 95531 hydrobromide (gabazine; 10 μM) (Alomone Labs, RRID:SCR_013570) were added to the extracellular solution to block inhibitory glycinergic and GABAergic currents, respectively .
Techniques: Injection, Control, Standard Deviation
Alomone Labs is a verified supplier 
Alomone Labs manufactures this product 
