symphony peptide synthesizer  (Gyros Protein Technologies)


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    Structured Review

    Gyros Protein Technologies symphony peptide synthesizer
    Symphony Peptide Synthesizer, supplied by Gyros Protein Technologies, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/symphony peptide synthesizer/product/Gyros Protein Technologies
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    symphony peptide synthesizer - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: ERAP1 enzyme-mediated trimming and structural analyses of MHC I–bound precursor peptides yield novel insights into antigen processing and presentation
    Article Snippet: .. Synthetic peptides Peptides were synthesized by the solid-phase methodology on a Symphony synthesizer (Protein Technologies Inc.) and purified by reverse-phase HPLC on a C18 Agilent column. ..

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA). .. The peptides were oxidized overnight in 0.1 M ammonium bicarbonate buffer at pH 8.5 and purified using reverse-phase HPLC (solvent A: H2 O, 0.05% (v /v ) trifluoroacetic acid (TFA), solvent B: 90% (v /v ) acetonitrile, 0.05% (v /v ) TFA) until the desired purity of > 95%.

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. Peptides were purified using reversed-phase high performance liquid chromatography (RP-HPLC) and the mass was confirmed using mass spectrometry.

    Mass Spectrometry:

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. Peptides were purified using reversed-phase high performance liquid chromatography (RP-HPLC) and the mass was confirmed using mass spectrometry.

    Ligation:

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.). .. The θ-defensin analogues were synthesized from C to N terminus, with the starting point for synthesis chosen to give an N-terminal cysteine residue (Cys14 for BTD-2, [Aba3,16 ]BTD-2, [Aba3,7,12,16 ]BTD-2, and l-BTD-2, and Cys16 for [Aba5,14 ]BTD-2 and [Aba5,7,12,14 ]BTD-2), to allow for cyclization by native chemical ligation ( ).

    Synthesized:

    Article Title: Novel Bcl-2 Homology-3 Domain-like Sequences Identified from Screening Randomized Peptide Libraries for Inhibitors of the Pro-survival Bcl-2 Proteins *
    Article Snippet: .. All regular peptides were synthesized on The Symphony® (Protein Technologies, Inc). ..

    Article Title: Interaction of Tarantula Venom Peptide ProTx-II with Lipid Membranes Is a Prerequisite for Its Inhibition of Human Voltage-gated Sodium Channel NaV1.7 *
    Article Snippet: .. ProTx-II and analogues ( ) were synthesized using Fmoc ( N -(9-fluorenyl)methoxycarbonyl) chemistry at 0.25-mmol scale on 2-chlorotrityl resin using an automatic synthesizer (Symphony, Protein Technologies Inc.). ..

    Article Title: ERAP1 enzyme-mediated trimming and structural analyses of MHC I–bound precursor peptides yield novel insights into antigen processing and presentation
    Article Snippet: .. Synthetic peptides Peptides were synthesized by the solid-phase methodology on a Symphony synthesizer (Protein Technologies Inc.) and purified by reverse-phase HPLC on a C18 Agilent column. ..

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: .. Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA). ..

    Article Title: Tag-Mass: Specific Molecular Imaging of Transcriptome and Proteome by Mass Spectrometry Based on Photocleavable Tag
    Article Snippet: .. The peptide is synthesized on Symphony (Protein Technologies, Inc.) and purified on a Delta-Pak C18, 15 μ m, 100 Å column (Waters). ..

    Article Title: Semienzymatic Cyclization of Disulfide-rich Peptides Using Sortase A *
    Article Snippet: .. The linear peptides [GGG]kB1[TGG], [G]Vc1.1[GLPETGGS], and [GG]SFTI-1[LPETGG] were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony®, Protein Technologies, Inc.) following standard Fmoc chemistry on a 2-chlorotrityl chloride resin. ..

    Article Title: Immunogenicity in mice and non-human primates of the Group A Streptococcal J8 peptide vaccine candidate conjugated to CRM197
    Article Snippet: .. Briefly, the peptide was synthesized by solid phase chemistry on a NovaPEG Rink Amide resin (0.62 mmeq/g, EMD Biosciences) using a Symphony Synthesizer (Protein Technologies, Inc.). ..

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: .. Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. 2-Chlorotrityl chloride resin was used for [GGG] PVIIA[LPETGG], whereas rink amide resin was used for linear κ-PVIIA to achieve C-terminal amidation. κ-PVIIA with N-terminal acetylation was produced by treating the resin with 6% acetic acid/0.2 M DIPEA in DMF following completion of elongation of the peptide.

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: .. The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.). .. The peptide chain was assembled on 2-chlorotrityl chloride resin (Novabiochem), piperidine was used for the deprotection step, and coupling of the Fmoc-protected amino acids was carried out as described for Boc synthesis.

    Purification:

    Article Title: ERAP1 enzyme-mediated trimming and structural analyses of MHC I–bound precursor peptides yield novel insights into antigen processing and presentation
    Article Snippet: .. Synthetic peptides Peptides were synthesized by the solid-phase methodology on a Symphony synthesizer (Protein Technologies Inc.) and purified by reverse-phase HPLC on a C18 Agilent column. ..

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: Paragraph title: 4.1. Peptide Synthesis, Folding and Purification ... Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA).

    Article Title: Tag-Mass: Specific Molecular Imaging of Transcriptome and Proteome by Mass Spectrometry Based on Photocleavable Tag
    Article Snippet: .. The peptide is synthesized on Symphony (Protein Technologies, Inc.) and purified on a Delta-Pak C18, 15 μ m, 100 Å column (Waters). ..

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. Peptides were purified using reversed-phase high performance liquid chromatography (RP-HPLC) and the mass was confirmed using mass spectrometry.

    Article Title: In Vivo Efficacy of Anuran Trypsin Inhibitory Peptides against Staphylococcal Skin Infection and the Impact of Peptide Cyclization
    Article Snippet: Paragraph title: Peptide synthesis and purification. ... 9-Fluorenylmethoxycarbonyl (Fmoc)-based chemistry was used to synthesize linear peptides on an automated synthesizer (Symphony; Protein Technologies, Inc.).

    Produced:

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. 2-Chlorotrityl chloride resin was used for [GGG] PVIIA[LPETGG], whereas rink amide resin was used for linear κ-PVIIA to achieve C-terminal amidation. κ-PVIIA with N-terminal acetylation was produced by treating the resin with 6% acetic acid/0.2 M DIPEA in DMF following completion of elongation of the peptide.

    Activation Assay:

    Article Title: Chemical Synthesis, Proper Folding, Nav Channel Selectivity Profile and Analgesic Properties of the Spider Peptide Phlotoxin 1
    Article Snippet: Chemical Synthesis of PhlTx1 Linear PhlTx1 was assembled stepwise using fmoc solid-phase chemistry on a Symphony Synthesizer (Protein Technologies Inc., Tucson, AZ, USA) at a 0.1 mmol scale on 2-chlorotrityl chloride resin (substitution approx. .. Fmoc protecting groups were removed using 20% piperidine in dimetilformamid (DMF) and free amine was coupled using tenfold excess of Fmoc amino acids and HCTU/DIEA activation in NMP/DMF (3 times 15 min).

    Nuclear Magnetic Resonance:

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA). .. The correct peptide mass was confirmed with ESI-MS, while native disulfide connectivity was inferred from the dispersion of peaks in the 1D NMR spectra using a Bruker Avance 600 MHz spectrometer (Billerica, USA).

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: Paragraph title: Peptide Synthesis and NMR Spectroscopy ... The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.).

    Spectroscopy:

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: Paragraph title: Peptide Synthesis and NMR Spectroscopy ... The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.).

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  • 99
    Gyros Protein Technologies symphony peptide synthesizer
    Symphony Peptide Synthesizer, supplied by Gyros Protein Technologies, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/symphony peptide synthesizer/product/Gyros Protein Technologies
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    symphony peptide synthesizer - by Bioz Stars, 2020-04
    99/100 stars
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