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Bio-Rad sybrgreen i
Sybrgreen I, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 81/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sybrgreen i/product/Bio-Rad
Average 81 stars, based on 2 article reviews
Price from $9.99 to $1999.99
sybrgreen i - by Bioz Stars, 2019-10
81/100 stars

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Related Articles

Amplification:

Article Title: Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
Article Snippet: For circularization, the reactions were incubated at 55 °C for 4 h, followed by five cycles of 95 °C for 1 min, and 55 °C for 4 h. To digest linear DNA after circularization, 2 μl exonuclease mix (containing 10 U/μl exonuclease I and 100 U/μl exonuclease III; USB) was added to the reaction, and the reactions were incubated at 37 °C for 2 h and then inactivated at 95 °C for 5 min. .. 10-μl circularization products were amplified by PCR in 100 μl reactions with 200 nM AmpF6.2-SoL primer, 200 nM AmpR6.2-SoL primer, 0.4× SybrGreen I and 50 μl iProof High-Fidelity Master Mix (Bio-Rad) at 98 °C for 30 s, eight cycles of 98 °C for 10 s, 58 °C for 20 s, 72 °C for 20 s, 14 cycles of 98 °C for 10 s, 72 °C for 20 s and 72 °C for 3 min. .. The amplicons of the expected size range (344–394 bp) were purified with 6% PAGE (6% TBE gel; Invitrogen).

Article Title: Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
Article Snippet: Approximately 100–500 ng of the end-repaired DNA was ligated with 60 μM Solexa sequencing adaptors in 30 μl of 1× QuickLigase Buffer (NEB) with 1 μl QuickLigase for 15 min at 25 °C. .. Ligation products of 150~175 bp in size were size selected with 6% PAGE, and amplified by PCR in 100 μl reactions with 15 μl template, 200 nM Solexa PCR primers, 0.8× SybrGreen I and 50 μl iProof High-Fidelity Master Mix (Bio-Rad) at 98 °C for 30 s, 12 cycles of 98 °C for 10 s, 65 °C for 20 s, 72 °C for 20 and 72 °C for 3 min. .. The PCR amplicons were purified with Qiaquick PCR purification columns, and sequenced on Illumina Genome Analyzer.

Ligation:

Article Title: Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
Article Snippet: Approximately 100–500 ng of the end-repaired DNA was ligated with 60 μM Solexa sequencing adaptors in 30 μl of 1× QuickLigase Buffer (NEB) with 1 μl QuickLigase for 15 min at 25 °C. .. Ligation products of 150~175 bp in size were size selected with 6% PAGE, and amplified by PCR in 100 μl reactions with 15 μl template, 200 nM Solexa PCR primers, 0.8× SybrGreen I and 50 μl iProof High-Fidelity Master Mix (Bio-Rad) at 98 °C for 30 s, 12 cycles of 98 °C for 10 s, 65 °C for 20 s, 72 °C for 20 and 72 °C for 3 min. .. The PCR amplicons were purified with Qiaquick PCR purification columns, and sequenced on Illumina Genome Analyzer.

Purification:

Article Title: Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
Article Snippet: The fragmented DNA was column purified, and end repaired at 25 °C for 45 min in 25-μl reactions containing 2.5 μl 10× buffer, 2.5 μl dNTP mix (2.5 mMeach), 2.5 μl ATP (10 mM), 1 μl end-repair enzyme mix (Epicentre), and 15 μl DNA. .. Ligation products of 150~175 bp in size were size selected with 6% PAGE, and amplified by PCR in 100 μl reactions with 15 μl template, 200 nM Solexa PCR primers, 0.8× SybrGreen I and 50 μl iProof High-Fidelity Master Mix (Bio-Rad) at 98 °C for 30 s, 12 cycles of 98 °C for 10 s, 65 °C for 20 s, 72 °C for 20 and 72 °C for 3 min.

Polymerase Chain Reaction:

Article Title: Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
Article Snippet: For circularization, the reactions were incubated at 55 °C for 4 h, followed by five cycles of 95 °C for 1 min, and 55 °C for 4 h. To digest linear DNA after circularization, 2 μl exonuclease mix (containing 10 U/μl exonuclease I and 100 U/μl exonuclease III; USB) was added to the reaction, and the reactions were incubated at 37 °C for 2 h and then inactivated at 95 °C for 5 min. .. 10-μl circularization products were amplified by PCR in 100 μl reactions with 200 nM AmpF6.2-SoL primer, 200 nM AmpR6.2-SoL primer, 0.4× SybrGreen I and 50 μl iProof High-Fidelity Master Mix (Bio-Rad) at 98 °C for 30 s, eight cycles of 98 °C for 10 s, 58 °C for 20 s, 72 °C for 20 s, 14 cycles of 98 °C for 10 s, 72 °C for 20 s and 72 °C for 3 min. .. The amplicons of the expected size range (344–394 bp) were purified with 6% PAGE (6% TBE gel; Invitrogen).

Article Title: Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
Article Snippet: Approximately 100–500 ng of the end-repaired DNA was ligated with 60 μM Solexa sequencing adaptors in 30 μl of 1× QuickLigase Buffer (NEB) with 1 μl QuickLigase for 15 min at 25 °C. .. Ligation products of 150~175 bp in size were size selected with 6% PAGE, and amplified by PCR in 100 μl reactions with 15 μl template, 200 nM Solexa PCR primers, 0.8× SybrGreen I and 50 μl iProof High-Fidelity Master Mix (Bio-Rad) at 98 °C for 30 s, 12 cycles of 98 °C for 10 s, 65 °C for 20 s, 72 °C for 20 and 72 °C for 3 min. .. The PCR amplicons were purified with Qiaquick PCR purification columns, and sequenced on Illumina Genome Analyzer.

Polyacrylamide Gel Electrophoresis:

Article Title: Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
Article Snippet: Approximately 100–500 ng of the end-repaired DNA was ligated with 60 μM Solexa sequencing adaptors in 30 μl of 1× QuickLigase Buffer (NEB) with 1 μl QuickLigase for 15 min at 25 °C. .. Ligation products of 150~175 bp in size were size selected with 6% PAGE, and amplified by PCR in 100 μl reactions with 15 μl template, 200 nM Solexa PCR primers, 0.8× SybrGreen I and 50 μl iProof High-Fidelity Master Mix (Bio-Rad) at 98 °C for 30 s, 12 cycles of 98 °C for 10 s, 65 °C for 20 s, 72 °C for 20 and 72 °C for 3 min. .. The PCR amplicons were purified with Qiaquick PCR purification columns, and sequenced on Illumina Genome Analyzer.

Sequencing:

Article Title: Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
Article Snippet: Approximately 100–500 ng of the end-repaired DNA was ligated with 60 μM Solexa sequencing adaptors in 30 μl of 1× QuickLigase Buffer (NEB) with 1 μl QuickLigase for 15 min at 25 °C. .. Ligation products of 150~175 bp in size were size selected with 6% PAGE, and amplified by PCR in 100 μl reactions with 15 μl template, 200 nM Solexa PCR primers, 0.8× SybrGreen I and 50 μl iProof High-Fidelity Master Mix (Bio-Rad) at 98 °C for 30 s, 12 cycles of 98 °C for 10 s, 65 °C for 20 s, 72 °C for 20 and 72 °C for 3 min.

Shotgun Sequencing:

Article Title: Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
Article Snippet: Paragraph title: Shotgun sequencing library construction ... Ligation products of 150~175 bp in size were size selected with 6% PAGE, and amplified by PCR in 100 μl reactions with 15 μl template, 200 nM Solexa PCR primers, 0.8× SybrGreen I and 50 μl iProof High-Fidelity Master Mix (Bio-Rad) at 98 °C for 30 s, 12 cycles of 98 °C for 10 s, 65 °C for 20 s, 72 °C for 20 and 72 °C for 3 min.

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    Bio-Rad sybrgreen i
    Sybrgreen I, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 81/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen i/product/Bio-Rad
    Average 81 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    81/100 stars
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