sybrgreen chemistry  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sybrgreen chemistry
    Sybrgreen Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen chemistry/product/Thermo Fisher
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    sybrgreen chemistry - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Cyclophilin A (CypA) Interacts with NF-κB Subunit, p65/RelA, and Contributes to NF-κB Activation Signaling
    Article Snippet: .. Using the cDNA synthesized from the SuperScript III First-Strand Synthesis System as templates, individual specific primers (p65 F, 5′-GACGACTGTTCCCCCTC-3′ and R, 5′-CCTCGCACTTGTAGCGG-3′ ; p50 F, 5′-GCAAACCTGGGAATACTTCATGTGACTAAG-3′ and R, 5′-ATAGGCAA-CCTCAGAATGCACCAGAAGTCC-3′ ; IL-1β F, 5′- TGCGTGTTGAAAGATGATAA-G-3′ and R, 5′- TTGGGGAACTGGGCAGAC-3′ ; IL-6 F, 5′-CTCCAGAACAGATTT-GAGAGTAGTG-3′ and R, 5′-TTGTGGTTGGGTCAGGGGTG-3′ ; IL-8 F, 5′-GGGTT-GTGGAGAAGTTTTTG-3′ and R, 5′-GTTTCACTGGCATCTTCACTG-3′ ) were added to each template and mixed with the Power SYBR Green PCR Master Mix (Life Technologies, Gaitherburg, MD). .. The reactions were performed using the 7300 Real-Time PCR System instrument (Applied Biosystems, Foster City, CA).

    Article Title: CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells
    Article Snippet: .. Equal volumes of prepared cDNA were used for quantification of HSV-1 gene ICP0 and ICP4 transcripts with the Power SYBR Green PCR master mix (Applied biosystems) according to the manufacturer's protocol. ..

    Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis
    Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Power SYBR green PCR master mix (Thermo Fisher Scientific) and Qiagen QuantiTect primer assays for human hypoxanthine phosphoribosyltransferase (HPRT) (QT00059066), APOBEC3G (QT00070770), SLC20A1 (PIT-1) (QT00028763), or SLC19A2 (THTR1) (QT00007847) on an ABI 7500 real-time PCR system. .. Quantification was carried out relative to HEK293 cells, and values were normalized to HPRT expression.

    Article Title: MicroRNA modulate alveolar epithelial response to cyclic stretch
    Article Snippet: .. Real-time PCR was performed (Taqman universal master mix or Applied Biosystems power SYBR green PCR master mix). ..

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

    Article Title: Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells
    Article Snippet: .. For mRNA analysis, total RNA was extracted from cells by TRIzol (Invitrogen) according to the manufacturer’s protocol and reverse-transcribed into cDNA using the QuantiTect® reverse transcription kit. cDNA was quantitated using Power SYBR Green PCR (Applied Biosystems by Life Technologies, Warrington, UK) and the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Article Title: Estradiol Protects Proopiomelanocortin Neurons Against Insulin Resistance
    Article Snippet: .. The results were as follows: Pik3cb (p110 β ), m = –3.481, r 2 = 0.95, efficiency = 96%; Gapdh , m = –3.35, r 2 = 0.99, efficiency = 98.7%; Socs3 , m = –3.294, r 2 = 0.90, efficiency = 100%; Trpc5 , m = –3.161, r 2 = 0.95, efficiency = 100%; Stim1 , m = –3.407, r 2 = 0.98, efficiency = 97%. qPCR was performed on a Quantstudio 7 Flex Real-Time PCR System (Life Technologies, Grand Island, NY) using Power SYBR Green Master Mix (Life Technologies) according to established protocols ( ). ..

    Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition
    Article Snippet: .. For quantitative RT-PCR (qRT-PCR), reverse-transcribed cDNA was used for real-time PCR using Power SYBR Green Master Mix (Applied Biosystems) with the HT7900 Fast Real Time PCR machine (Applied Biosystems). .. Standard curves were generated using serial dilutions of cDNA with arbitrary copy numbers.

    Amplification:

    Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis
    Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Power SYBR green PCR master mix (Thermo Fisher Scientific) and Qiagen QuantiTect primer assays for human hypoxanthine phosphoribosyltransferase (HPRT) (QT00059066), APOBEC3G (QT00070770), SLC20A1 (PIT-1) (QT00028763), or SLC19A2 (THTR1) (QT00007847) on an ABI 7500 real-time PCR system. .. Quantification was carried out relative to HEK293 cells, and values were normalized to HPRT expression.

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

    Synthesized:

    Article Title: Cyclophilin A (CypA) Interacts with NF-κB Subunit, p65/RelA, and Contributes to NF-κB Activation Signaling
    Article Snippet: .. Using the cDNA synthesized from the SuperScript III First-Strand Synthesis System as templates, individual specific primers (p65 F, 5′-GACGACTGTTCCCCCTC-3′ and R, 5′-CCTCGCACTTGTAGCGG-3′ ; p50 F, 5′-GCAAACCTGGGAATACTTCATGTGACTAAG-3′ and R, 5′-ATAGGCAA-CCTCAGAATGCACCAGAAGTCC-3′ ; IL-1β F, 5′- TGCGTGTTGAAAGATGATAA-G-3′ and R, 5′- TTGGGGAACTGGGCAGAC-3′ ; IL-6 F, 5′-CTCCAGAACAGATTT-GAGAGTAGTG-3′ and R, 5′-TTGTGGTTGGGTCAGGGGTG-3′ ; IL-8 F, 5′-GGGTT-GTGGAGAAGTTTTTG-3′ and R, 5′-GTTTCACTGGCATCTTCACTG-3′ ) were added to each template and mixed with the Power SYBR Green PCR Master Mix (Life Technologies, Gaitherburg, MD). .. The reactions were performed using the 7300 Real-Time PCR System instrument (Applied Biosystems, Foster City, CA).

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

    Quantitative RT-PCR:

    Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition
    Article Snippet: .. For quantitative RT-PCR (qRT-PCR), reverse-transcribed cDNA was used for real-time PCR using Power SYBR Green Master Mix (Applied Biosystems) with the HT7900 Fast Real Time PCR machine (Applied Biosystems). .. Standard curves were generated using serial dilutions of cDNA with arbitrary copy numbers.

    Real-time Polymerase Chain Reaction:

    Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis
    Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Power SYBR green PCR master mix (Thermo Fisher Scientific) and Qiagen QuantiTect primer assays for human hypoxanthine phosphoribosyltransferase (HPRT) (QT00059066), APOBEC3G (QT00070770), SLC20A1 (PIT-1) (QT00028763), or SLC19A2 (THTR1) (QT00007847) on an ABI 7500 real-time PCR system. .. Quantification was carried out relative to HEK293 cells, and values were normalized to HPRT expression.

    Article Title: MicroRNA modulate alveolar epithelial response to cyclic stretch
    Article Snippet: .. Real-time PCR was performed (Taqman universal master mix or Applied Biosystems power SYBR green PCR master mix). ..

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

    Article Title: Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells
    Article Snippet: .. For mRNA analysis, total RNA was extracted from cells by TRIzol (Invitrogen) according to the manufacturer’s protocol and reverse-transcribed into cDNA using the QuantiTect® reverse transcription kit. cDNA was quantitated using Power SYBR Green PCR (Applied Biosystems by Life Technologies, Warrington, UK) and the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Article Title: Estradiol Protects Proopiomelanocortin Neurons Against Insulin Resistance
    Article Snippet: .. The results were as follows: Pik3cb (p110 β ), m = –3.481, r 2 = 0.95, efficiency = 96%; Gapdh , m = –3.35, r 2 = 0.99, efficiency = 98.7%; Socs3 , m = –3.294, r 2 = 0.90, efficiency = 100%; Trpc5 , m = –3.161, r 2 = 0.95, efficiency = 100%; Stim1 , m = –3.407, r 2 = 0.98, efficiency = 97%. qPCR was performed on a Quantstudio 7 Flex Real-Time PCR System (Life Technologies, Grand Island, NY) using Power SYBR Green Master Mix (Life Technologies) according to established protocols ( ). ..

    Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition
    Article Snippet: .. For quantitative RT-PCR (qRT-PCR), reverse-transcribed cDNA was used for real-time PCR using Power SYBR Green Master Mix (Applied Biosystems) with the HT7900 Fast Real Time PCR machine (Applied Biosystems). .. Standard curves were generated using serial dilutions of cDNA with arbitrary copy numbers.

    Polymerase Chain Reaction:

    Article Title: Cyclophilin A (CypA) Interacts with NF-κB Subunit, p65/RelA, and Contributes to NF-κB Activation Signaling
    Article Snippet: .. Using the cDNA synthesized from the SuperScript III First-Strand Synthesis System as templates, individual specific primers (p65 F, 5′-GACGACTGTTCCCCCTC-3′ and R, 5′-CCTCGCACTTGTAGCGG-3′ ; p50 F, 5′-GCAAACCTGGGAATACTTCATGTGACTAAG-3′ and R, 5′-ATAGGCAA-CCTCAGAATGCACCAGAAGTCC-3′ ; IL-1β F, 5′- TGCGTGTTGAAAGATGATAA-G-3′ and R, 5′- TTGGGGAACTGGGCAGAC-3′ ; IL-6 F, 5′-CTCCAGAACAGATTT-GAGAGTAGTG-3′ and R, 5′-TTGTGGTTGGGTCAGGGGTG-3′ ; IL-8 F, 5′-GGGTT-GTGGAGAAGTTTTTG-3′ and R, 5′-GTTTCACTGGCATCTTCACTG-3′ ) were added to each template and mixed with the Power SYBR Green PCR Master Mix (Life Technologies, Gaitherburg, MD). .. The reactions were performed using the 7300 Real-Time PCR System instrument (Applied Biosystems, Foster City, CA).

    Article Title: CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells
    Article Snippet: .. Equal volumes of prepared cDNA were used for quantification of HSV-1 gene ICP0 and ICP4 transcripts with the Power SYBR Green PCR master mix (Applied biosystems) according to the manufacturer's protocol. ..

    Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis
    Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Power SYBR green PCR master mix (Thermo Fisher Scientific) and Qiagen QuantiTect primer assays for human hypoxanthine phosphoribosyltransferase (HPRT) (QT00059066), APOBEC3G (QT00070770), SLC20A1 (PIT-1) (QT00028763), or SLC19A2 (THTR1) (QT00007847) on an ABI 7500 real-time PCR system. .. Quantification was carried out relative to HEK293 cells, and values were normalized to HPRT expression.

    Article Title: MicroRNA modulate alveolar epithelial response to cyclic stretch
    Article Snippet: .. Real-time PCR was performed (Taqman universal master mix or Applied Biosystems power SYBR green PCR master mix). ..

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

    Article Title: Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells
    Article Snippet: .. For mRNA analysis, total RNA was extracted from cells by TRIzol (Invitrogen) according to the manufacturer’s protocol and reverse-transcribed into cDNA using the QuantiTect® reverse transcription kit. cDNA was quantitated using Power SYBR Green PCR (Applied Biosystems by Life Technologies, Warrington, UK) and the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

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  • About
  • News
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  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Thermo Fisher sybrgreen chemistry
    Sybrgreen Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen chemistry/product/Thermo Fisher
    Average 91 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    sybrgreen chemistry - by Bioz Stars, 2020-08
    91/100 stars
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