sybrgreen chemistry (Thermo Fisher)
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Thermo Fisher
sybrgreen chemistry
Sybrgreen Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sybrgreen chemistry/product/Thermo Fisher
Average 99 stars, based on 36 article reviews
Price from $9.99 to $1999.99
Sybrgreen Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sybrgreen chemistry/product/Thermo Fisher
Average 99 stars, based on 36 article reviews
Price from $9.99 to $1999.99
sybrgreen chemistry - by Bioz Stars,
2020-08
99/100 stars
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SYBR Green Assay:Article Title: Cyclophilin A (CypA) Interacts with NF-κB Subunit, p65/RelA, and Contributes to NF-κB Activation Signaling Article Snippet: .. Using the cDNA synthesized from the SuperScript III First-Strand Synthesis System as templates, individual specific primers (p65 F, 5′-GACGACTGTTCCCCCTC-3′ and R, 5′-CCTCGCACTTGTAGCGG-3′ ; p50 F, 5′-GCAAACCTGGGAATACTTCATGTGACTAAG-3′ and R, 5′-ATAGGCAA-CCTCAGAATGCACCAGAAGTCC-3′ ; IL-1β F, 5′- TGCGTGTTGAAAGATGATAA-G-3′ and R, 5′- TTGGGGAACTGGGCAGAC-3′ ; IL-6 F, 5′-CTCCAGAACAGATTT-GAGAGTAGTG-3′ and R, 5′-TTGTGGTTGGGTCAGGGGTG-3′ ; IL-8 F, 5′-GGGTT-GTGGAGAAGTTTTTG-3′ and R, 5′-GTTTCACTGGCATCTTCACTG-3′ ) were added to each template and mixed with the Article Title: CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells Article Snippet: .. Equal volumes of prepared cDNA were used for quantification of HSV-1 gene ICP0 and ICP4 transcripts with the Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Article Title: MicroRNA modulate alveolar epithelial response to cyclic stretch Article Snippet: .. Real-time PCR was performed (Taqman universal master mix or Applied Biosystems Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Article Title: Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells Article Snippet: .. For mRNA analysis, total RNA was extracted from cells by TRIzol (Invitrogen) according to the manufacturer’s protocol and reverse-transcribed into cDNA using the QuantiTect® reverse transcription kit. cDNA was quantitated using Article Title: Estradiol Protects Proopiomelanocortin Neurons Against Insulin Resistance Article Snippet: .. The results were as follows: Pik3cb (p110 β ), m = –3.481, r 2 = 0.95, efficiency = 96%; Gapdh , m = –3.35, r 2 = 0.99, efficiency = 98.7%; Socs3 , m = –3.294, r 2 = 0.90, efficiency = 100%; Trpc5 , m = –3.161, r 2 = 0.95, efficiency = 100%; Stim1 , m = –3.407, r 2 = 0.98, efficiency = 97%. qPCR was performed on a Quantstudio 7 Flex Real-Time PCR System (Life Technologies, Grand Island, NY) using Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition Article Snippet: .. For quantitative RT-PCR (qRT-PCR), reverse-transcribed cDNA was used for real-time PCR using Amplification:Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Synthesized:Article Title: Cyclophilin A (CypA) Interacts with NF-κB Subunit, p65/RelA, and Contributes to NF-κB Activation Signaling Article Snippet: .. Using the cDNA synthesized from the SuperScript III First-Strand Synthesis System as templates, individual specific primers (p65 F, 5′-GACGACTGTTCCCCCTC-3′ and R, 5′-CCTCGCACTTGTAGCGG-3′ ; p50 F, 5′-GCAAACCTGGGAATACTTCATGTGACTAAG-3′ and R, 5′-ATAGGCAA-CCTCAGAATGCACCAGAAGTCC-3′ ; IL-1β F, 5′- TGCGTGTTGAAAGATGATAA-G-3′ and R, 5′- TTGGGGAACTGGGCAGAC-3′ ; IL-6 F, 5′-CTCCAGAACAGATTT-GAGAGTAGTG-3′ and R, 5′-TTGTGGTTGGGTCAGGGGTG-3′ ; IL-8 F, 5′-GGGTT-GTGGAGAAGTTTTTG-3′ and R, 5′-GTTTCACTGGCATCTTCACTG-3′ ) were added to each template and mixed with the Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Quantitative RT-PCR:Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition Article Snippet: .. For quantitative RT-PCR (qRT-PCR), reverse-transcribed cDNA was used for real-time PCR using Real-time Polymerase Chain Reaction:Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Article Title: MicroRNA modulate alveolar epithelial response to cyclic stretch Article Snippet: .. Real-time PCR was performed (Taqman universal master mix or Applied Biosystems Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Article Title: Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells Article Snippet: .. For mRNA analysis, total RNA was extracted from cells by TRIzol (Invitrogen) according to the manufacturer’s protocol and reverse-transcribed into cDNA using the QuantiTect® reverse transcription kit. cDNA was quantitated using Article Title: Estradiol Protects Proopiomelanocortin Neurons Against Insulin Resistance Article Snippet: .. The results were as follows: Pik3cb (p110 β ), m = –3.481, r 2 = 0.95, efficiency = 96%; Gapdh , m = –3.35, r 2 = 0.99, efficiency = 98.7%; Socs3 , m = –3.294, r 2 = 0.90, efficiency = 100%; Trpc5 , m = –3.161, r 2 = 0.95, efficiency = 100%; Stim1 , m = –3.407, r 2 = 0.98, efficiency = 97%. qPCR was performed on a Quantstudio 7 Flex Real-Time PCR System (Life Technologies, Grand Island, NY) using Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition Article Snippet: .. For quantitative RT-PCR (qRT-PCR), reverse-transcribed cDNA was used for real-time PCR using Polymerase Chain Reaction:Article Title: Cyclophilin A (CypA) Interacts with NF-κB Subunit, p65/RelA, and Contributes to NF-κB Activation Signaling Article Snippet: .. Using the cDNA synthesized from the SuperScript III First-Strand Synthesis System as templates, individual specific primers (p65 F, 5′-GACGACTGTTCCCCCTC-3′ and R, 5′-CCTCGCACTTGTAGCGG-3′ ; p50 F, 5′-GCAAACCTGGGAATACTTCATGTGACTAAG-3′ and R, 5′-ATAGGCAA-CCTCAGAATGCACCAGAAGTCC-3′ ; IL-1β F, 5′- TGCGTGTTGAAAGATGATAA-G-3′ and R, 5′- TTGGGGAACTGGGCAGAC-3′ ; IL-6 F, 5′-CTCCAGAACAGATTT-GAGAGTAGTG-3′ and R, 5′-TTGTGGTTGGGTCAGGGGTG-3′ ; IL-8 F, 5′-GGGTT-GTGGAGAAGTTTTTG-3′ and R, 5′-GTTTCACTGGCATCTTCACTG-3′ ) were added to each template and mixed with the Article Title: CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells Article Snippet: .. Equal volumes of prepared cDNA were used for quantification of HSV-1 gene ICP0 and ICP4 transcripts with the Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Article Title: MicroRNA modulate alveolar epithelial response to cyclic stretch Article Snippet: .. Real-time PCR was performed (Taqman universal master mix or Applied Biosystems Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Article Title: Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells Article Snippet: .. For mRNA analysis, total RNA was extracted from cells by TRIzol (Invitrogen) according to the manufacturer’s protocol and reverse-transcribed into cDNA using the QuantiTect® reverse transcription kit. cDNA was quantitated using Reverse Transcription Polymerase Chain Reaction:Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with |