sybr green polymerase chain reaction master mix  (Roche)

 
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    Structured Review

    Roche sybr green polymerase chain reaction master mix
    Sybr Green Polymerase Chain Reaction Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green polymerase chain reaction master mix/product/Roche
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr green polymerase chain reaction master mix - by Bioz Stars, 2020-08
    92/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: RNA Oncoimmune Phenotyping of HPV-Positive p16-Positive Oropharyngeal Squamous Cell Carcinomas by Nodal Status
    Article Snippet: .. Reverse-transcription reactions were performed with the Transcriptor First-Strand Synthesis kit (Roche AG) using 1 μg of total RNA, 60 μM of random hexamers, and 2.5 μM of oligo-dT primers, and expression of the indicated genes was analyzed by quantitative reverse transcription polymerase chain reaction using a QuantStudio 6 Flex Real Time PCR System (Applied Biosystems) using SYBR green polymerase chain reaction master mix (Roche AG). .. Each reaction mixture contained 1 × SYBR green master mix, complementary DNA from 1 μg of RNA, and 0.3 μM of each oligonucleotide primer in a total volume of 20 μL.

    Amplification:

    Article Title: IL-33-driven ILC2/eosinophil Axis in Fat Is Induced by Sympathetic Tone and Suppressed by Obesity
    Article Snippet: .. PCR amplification was detected using SYBR Green PCR master mixture on Roche 480 Real-time PCR system (Roche). .. The primer sequences were as follows: Arginase 1 forward primer 5′-CAGCTACCTGCTGGGAAGGAAGAA-3′ and reverse primer 5′-CCAAGAGTTGGGTTCACTTCCA-3′; CD206 forward primer 5′-TGTGGTGAGCTGAAAGGTGA-3′ and reverse primer 5′-CAGGTGTGGGCGCAGGTAG-3′; F4/80 forward primer 5′-CTTTGGCTATGGGCTTCCAGTC-3′ and reverse primer 5′-GCAAGGAGGACAGAGTTTATCGTG-3′; Nr3c2 forward primer 5′-GAAGAGCCCCTCTGTTTGCAG-3′ and reverse primer 5′-TCCTTGAGTGATGGGACTGTG-3′; IL33 forward primer 5′-TGAGACTCCGTTCTGGCCTC-3′ and reverse primer 5′-CTCTTCATGCTTGGTACCCGAT-3′; GAPDH forward primer 5′-CGTGCCGCCTGGAGAAA CC-3′ and reverse primer 5′-TGGAAGAGTGGGAGTTGCTGTTG-3′.

    Agarose Gel Electrophoresis:

    Article Title: Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress
    Article Snippet: .. PCR products were analyzed on a 2 % (w/v) agarose gel. qRT-PCR were performed at 95 °C for 10 min, and then 40 cycles of incubation at 95 °C for 10 s, 55–60 °C 10 s and 72 °C for 6 s in a LightCycler480 machine using SYBR Green PCR master mixture (Roche). .. A melting curve analysis was applied to check PCR specificity.

    Quantitative RT-PCR:

    Article Title: Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress
    Article Snippet: .. PCR products were analyzed on a 2 % (w/v) agarose gel. qRT-PCR were performed at 95 °C for 10 min, and then 40 cycles of incubation at 95 °C for 10 s, 55–60 °C 10 s and 72 °C for 6 s in a LightCycler480 machine using SYBR Green PCR master mixture (Roche). .. A melting curve analysis was applied to check PCR specificity.

    Article Title: Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis
    Article Snippet: .. Total RNA (1 µg) treated with DNaseI (New England Biolabs, Inc., Ipswich, MA, USA) was used for synthesizing first-stranded cDNA with the oligo-dT primer using reverse transcriptase (Roche, Basel, Germany) in accordance with the manufacturer’s instructions. qRT–PCR was performed using LightCycler 480 system (Roche Applied Science, Indianapolis, IN, USA) and SYBR Green PCR master mixture (Roche, Basel, Germany). .. Real-time PCR amplification efficiency of our qRT–PCR primers was at least 1.8.

    SYBR Green Assay:

    Article Title: RNA Oncoimmune Phenotyping of HPV-Positive p16-Positive Oropharyngeal Squamous Cell Carcinomas by Nodal Status
    Article Snippet: .. Reverse-transcription reactions were performed with the Transcriptor First-Strand Synthesis kit (Roche AG) using 1 μg of total RNA, 60 μM of random hexamers, and 2.5 μM of oligo-dT primers, and expression of the indicated genes was analyzed by quantitative reverse transcription polymerase chain reaction using a QuantStudio 6 Flex Real Time PCR System (Applied Biosystems) using SYBR green polymerase chain reaction master mix (Roche AG). .. Each reaction mixture contained 1 × SYBR green master mix, complementary DNA from 1 μg of RNA, and 0.3 μM of each oligonucleotide primer in a total volume of 20 μL.

    Article Title: Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress
    Article Snippet: .. PCR products were analyzed on a 2 % (w/v) agarose gel. qRT-PCR were performed at 95 °C for 10 min, and then 40 cycles of incubation at 95 °C for 10 s, 55–60 °C 10 s and 72 °C for 6 s in a LightCycler480 machine using SYBR Green PCR master mixture (Roche). .. A melting curve analysis was applied to check PCR specificity.

    Article Title: Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis
    Article Snippet: .. Total RNA (1 µg) treated with DNaseI (New England Biolabs, Inc., Ipswich, MA, USA) was used for synthesizing first-stranded cDNA with the oligo-dT primer using reverse transcriptase (Roche, Basel, Germany) in accordance with the manufacturer’s instructions. qRT–PCR was performed using LightCycler 480 system (Roche Applied Science, Indianapolis, IN, USA) and SYBR Green PCR master mixture (Roche, Basel, Germany). .. Real-time PCR amplification efficiency of our qRT–PCR primers was at least 1.8.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: RNA Oncoimmune Phenotyping of HPV-Positive p16-Positive Oropharyngeal Squamous Cell Carcinomas by Nodal Status
    Article Snippet: .. Reverse-transcription reactions were performed with the Transcriptor First-Strand Synthesis kit (Roche AG) using 1 μg of total RNA, 60 μM of random hexamers, and 2.5 μM of oligo-dT primers, and expression of the indicated genes was analyzed by quantitative reverse transcription polymerase chain reaction using a QuantStudio 6 Flex Real Time PCR System (Applied Biosystems) using SYBR green polymerase chain reaction master mix (Roche AG). .. Each reaction mixture contained 1 × SYBR green master mix, complementary DNA from 1 μg of RNA, and 0.3 μM of each oligonucleotide primer in a total volume of 20 μL.

    Incubation:

    Article Title: Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress
    Article Snippet: .. PCR products were analyzed on a 2 % (w/v) agarose gel. qRT-PCR were performed at 95 °C for 10 min, and then 40 cycles of incubation at 95 °C for 10 s, 55–60 °C 10 s and 72 °C for 6 s in a LightCycler480 machine using SYBR Green PCR master mixture (Roche). .. A melting curve analysis was applied to check PCR specificity.

    Expressing:

    Article Title: RNA Oncoimmune Phenotyping of HPV-Positive p16-Positive Oropharyngeal Squamous Cell Carcinomas by Nodal Status
    Article Snippet: .. Reverse-transcription reactions were performed with the Transcriptor First-Strand Synthesis kit (Roche AG) using 1 μg of total RNA, 60 μM of random hexamers, and 2.5 μM of oligo-dT primers, and expression of the indicated genes was analyzed by quantitative reverse transcription polymerase chain reaction using a QuantStudio 6 Flex Real Time PCR System (Applied Biosystems) using SYBR green polymerase chain reaction master mix (Roche AG). .. Each reaction mixture contained 1 × SYBR green master mix, complementary DNA from 1 μg of RNA, and 0.3 μM of each oligonucleotide primer in a total volume of 20 μL.

    Polymerase Chain Reaction:

    Article Title: RNA Oncoimmune Phenotyping of HPV-Positive p16-Positive Oropharyngeal Squamous Cell Carcinomas by Nodal Status
    Article Snippet: .. Reverse-transcription reactions were performed with the Transcriptor First-Strand Synthesis kit (Roche AG) using 1 μg of total RNA, 60 μM of random hexamers, and 2.5 μM of oligo-dT primers, and expression of the indicated genes was analyzed by quantitative reverse transcription polymerase chain reaction using a QuantStudio 6 Flex Real Time PCR System (Applied Biosystems) using SYBR green polymerase chain reaction master mix (Roche AG). .. Each reaction mixture contained 1 × SYBR green master mix, complementary DNA from 1 μg of RNA, and 0.3 μM of each oligonucleotide primer in a total volume of 20 μL.

    Article Title: Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress
    Article Snippet: .. PCR products were analyzed on a 2 % (w/v) agarose gel. qRT-PCR were performed at 95 °C for 10 min, and then 40 cycles of incubation at 95 °C for 10 s, 55–60 °C 10 s and 72 °C for 6 s in a LightCycler480 machine using SYBR Green PCR master mixture (Roche). .. A melting curve analysis was applied to check PCR specificity.

    Article Title: Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis
    Article Snippet: .. Total RNA (1 µg) treated with DNaseI (New England Biolabs, Inc., Ipswich, MA, USA) was used for synthesizing first-stranded cDNA with the oligo-dT primer using reverse transcriptase (Roche, Basel, Germany) in accordance with the manufacturer’s instructions. qRT–PCR was performed using LightCycler 480 system (Roche Applied Science, Indianapolis, IN, USA) and SYBR Green PCR master mixture (Roche, Basel, Germany). .. Real-time PCR amplification efficiency of our qRT–PCR primers was at least 1.8.

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    Roche faststart universal sybr green master rox mix
    Quantification of SRC-2 in the quail brain by quantitative PCR using the <t>FastStart</t> Universal <t>SYBR</t> Green Master (ROX) Mix. Bar graphs illustrate the SRC-2/GAPDH ratio in the medial preoptic nucleus (POM), cerebellum and optic lobes of male (white) and
    Faststart Universal Sybr Green Master Rox Mix, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/faststart universal sybr green master rox mix/product/Roche
    Average 89 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    faststart universal sybr green master rox mix - by Bioz Stars, 2020-08
    89/100 stars
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    89
    Roche sybr green qrt pcr master mix
    X4 gp120 induction of a-SMA and collagen I expression is CXCR4-dependent. (A) Passage #3 primary HSCs were plated at a density of 2×10 4 cells/well in 6-well plates, serum-starved for 24 hours, and then transfected with shCXCR4 or shControl. A 75% reduction in CXCR4 protein expression was noted 72 hours after transfection by Western blot. (B) 72 hours after shControl or sh CXCR4 transfection, primary HSCs were then challenged with X4 gp120 for 2 hours, RNA harvested, reverse transcribed and <t>qRT-PCR</t> performed for CXCR4, a-SMA, and coll I (a1). A 40–50% reduction in gp120-induced collagen (a1) and a-SMA mRNA levels was observed with CXCR4 knockdown. Data are expressed as the mean ± standard deviation of three independent experiments. (C) 72 hours after shCXCR4 knockdown, HSCs were challenged with HIV-IIIB for 8 hours and cell lysates used for Western blot where a 70% reduction in the protein expression of both a-SMA and collagen I was observed. (D) Human primary HSCs were pretreated with anti-CXCR4 antibody for 30 min (20 µg/ml) followed by treatment with gp120 (500 ng/mL) for 8 hours. Both X4 gp120-induced a-SMA and coll I protein expression were attenuated by anti-CXCR4 neutralizing antibody. ß-actin, a-tubulin, and GAPDH were used as loading controls. Representative Western blots with normalized densitometric arbitary units shown.
    Sybr Green Qrt Pcr Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green qrt pcr master mix/product/Roche
    Average 89 stars, based on 3 article reviews
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    sybr green qrt pcr master mix - by Bioz Stars, 2020-08
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    94
    Roche sybr green master mix
    PZ-235 suppresses liver expression of inflammatory cytokines in CCl 4 -treated mice receiving delayed pepducin treatment initiated at week 4. Livers were isolated from mice in at week 8, and quantitative <t>PCR</t> by <t>SYBR</t> Green (mean ± S.E., n
    Sybr Green Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green master mix/product/Roche
    Average 94 stars, based on 117 article reviews
    Price from $9.99 to $1999.99
    sybr green master mix - by Bioz Stars, 2020-08
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    93
    Roche sybr green quantitative pcr qpcr master mix
    YE, R5, and HC cells regulated the mRNA levels of cytokines-IL-10, IL-17A, IL-17F, and TNF-α-and transcription factors-Foxp3, and RORγ-in ConA-induced ALF mice. The regulatory effects of YE and R5 cells were significantly stronger than those of HC cells, with YE cells exhibiting the most optimal regulation. Quantitative <t>PCR</t> <t>(qPCR)</t> was used to detect the mRNA levels of IL-10 (A), IL-17A (B), IL-17F (C), TNF-α (D), Foxp3 (E), and RORγt (F) in the livers of Normal, ConA, YE, R5, and HC groups of mice. Data are presented as the mean ± SEM (n = 8; * P
    Sybr Green Quantitative Pcr Qpcr Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green quantitative pcr qpcr master mix/product/Roche
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr green quantitative pcr qpcr master mix - by Bioz Stars, 2020-08
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    Image Search Results


    Quantification of SRC-2 in the quail brain by quantitative PCR using the FastStart Universal SYBR Green Master (ROX) Mix. Bar graphs illustrate the SRC-2/GAPDH ratio in the medial preoptic nucleus (POM), cerebellum and optic lobes of male (white) and

    Journal: Journal of neurochemistry

    Article Title: STEROID RECEPTOR COACTIVATOR 2 (SRC-2) MODULATES STEROID-DEPENDENT MALE SEXUAL BEHAVIOR AND NEUROPLASTICITY IN JAPANESE QUAIL (COTURNIX JAPONICA)

    doi: 10.1111/j.1471-4159.2011.07438.x

    Figure Lengend Snippet: Quantification of SRC-2 in the quail brain by quantitative PCR using the FastStart Universal SYBR Green Master (ROX) Mix. Bar graphs illustrate the SRC-2/GAPDH ratio in the medial preoptic nucleus (POM), cerebellum and optic lobes of male (white) and

    Article Snippet: Isolated punches were then processed for RNA isolation in 1 ml of Trizol reagent according to the manufacturers protocol (Invitrogen). qRT-PCR was performed on a Westburg Rotor-Gene RG-3000 using the FastStart Universal SYBR Green Master (ROX) Mix (Roche Diagnostics, Mannheim, Germany) to quantify the transcription level of SRC-2 in the 3 brain areas in male and female quail.

    Techniques: Real-time Polymerase Chain Reaction, SYBR Green Assay

    X4 gp120 induction of a-SMA and collagen I expression is CXCR4-dependent. (A) Passage #3 primary HSCs were plated at a density of 2×10 4 cells/well in 6-well plates, serum-starved for 24 hours, and then transfected with shCXCR4 or shControl. A 75% reduction in CXCR4 protein expression was noted 72 hours after transfection by Western blot. (B) 72 hours after shControl or sh CXCR4 transfection, primary HSCs were then challenged with X4 gp120 for 2 hours, RNA harvested, reverse transcribed and qRT-PCR performed for CXCR4, a-SMA, and coll I (a1). A 40–50% reduction in gp120-induced collagen (a1) and a-SMA mRNA levels was observed with CXCR4 knockdown. Data are expressed as the mean ± standard deviation of three independent experiments. (C) 72 hours after shCXCR4 knockdown, HSCs were challenged with HIV-IIIB for 8 hours and cell lysates used for Western blot where a 70% reduction in the protein expression of both a-SMA and collagen I was observed. (D) Human primary HSCs were pretreated with anti-CXCR4 antibody for 30 min (20 µg/ml) followed by treatment with gp120 (500 ng/mL) for 8 hours. Both X4 gp120-induced a-SMA and coll I protein expression were attenuated by anti-CXCR4 neutralizing antibody. ß-actin, a-tubulin, and GAPDH were used as loading controls. Representative Western blots with normalized densitometric arbitary units shown.

    Journal: PLoS ONE

    Article Title: X4 Human Immunodeficiency Virus Type 1 gp120 Promotes Human Hepatic Stellate Cell Activation and Collagen I Expression through Interactions with CXCR4

    doi: 10.1371/journal.pone.0033659

    Figure Lengend Snippet: X4 gp120 induction of a-SMA and collagen I expression is CXCR4-dependent. (A) Passage #3 primary HSCs were plated at a density of 2×10 4 cells/well in 6-well plates, serum-starved for 24 hours, and then transfected with shCXCR4 or shControl. A 75% reduction in CXCR4 protein expression was noted 72 hours after transfection by Western blot. (B) 72 hours after shControl or sh CXCR4 transfection, primary HSCs were then challenged with X4 gp120 for 2 hours, RNA harvested, reverse transcribed and qRT-PCR performed for CXCR4, a-SMA, and coll I (a1). A 40–50% reduction in gp120-induced collagen (a1) and a-SMA mRNA levels was observed with CXCR4 knockdown. Data are expressed as the mean ± standard deviation of three independent experiments. (C) 72 hours after shCXCR4 knockdown, HSCs were challenged with HIV-IIIB for 8 hours and cell lysates used for Western blot where a 70% reduction in the protein expression of both a-SMA and collagen I was observed. (D) Human primary HSCs were pretreated with anti-CXCR4 antibody for 30 min (20 µg/ml) followed by treatment with gp120 (500 ng/mL) for 8 hours. Both X4 gp120-induced a-SMA and coll I protein expression were attenuated by anti-CXCR4 neutralizing antibody. ß-actin, a-tubulin, and GAPDH were used as loading controls. Representative Western blots with normalized densitometric arbitary units shown.

    Article Snippet: Reverse-Transcription and Real-time quantitative PCR (qRT-PCR) RNA was extracted from the cells and reverse transcribed into cDNA using RNeasy® kit (Qiagen, Valencia, CA) and Omniscript RT Kit (Qiagen), respectively. mRNA levels were analyzed by qRT-PCR using SYBR green qRT-PCR Master Mix (Roche) on the LightCycler®480 System (Roche).

    Techniques: Expressing, Transfection, Western Blot, Quantitative RT-PCR, Standard Deviation

    X4 HIV-1 gp120 induces fibrogenic gene expression in human stellate cells. (A) LX-2 cells were serum-starved for 24 hrs, treated with X4 HIV-1 gp120 at a final concentration of 500 ng/ml for 2 hours, RNA harvested, reverse transcribed, and qRT-PCR performed for TGF-ß1, type I TGF-ß receptor, a-SMA and coll I (a1) mRNA levels. To confirm X4 gp120 effects were CD4-independent, cells were pre-incubated with 25 µg/mL anti-CD4 30 minutes prior to challenge with X4 gp120. (B, C) Effect on collagen I (a1) was confirmed in primary HSCs with both X4 gp120 as well as AT-2 treated X4-tropic HIV-IIIB, which presents gp120 in its oligomeric confirmation. All data are expressed as means +/− standard deviation of at least three independent experiments.

    Journal: PLoS ONE

    Article Title: X4 Human Immunodeficiency Virus Type 1 gp120 Promotes Human Hepatic Stellate Cell Activation and Collagen I Expression through Interactions with CXCR4

    doi: 10.1371/journal.pone.0033659

    Figure Lengend Snippet: X4 HIV-1 gp120 induces fibrogenic gene expression in human stellate cells. (A) LX-2 cells were serum-starved for 24 hrs, treated with X4 HIV-1 gp120 at a final concentration of 500 ng/ml for 2 hours, RNA harvested, reverse transcribed, and qRT-PCR performed for TGF-ß1, type I TGF-ß receptor, a-SMA and coll I (a1) mRNA levels. To confirm X4 gp120 effects were CD4-independent, cells were pre-incubated with 25 µg/mL anti-CD4 30 minutes prior to challenge with X4 gp120. (B, C) Effect on collagen I (a1) was confirmed in primary HSCs with both X4 gp120 as well as AT-2 treated X4-tropic HIV-IIIB, which presents gp120 in its oligomeric confirmation. All data are expressed as means +/− standard deviation of at least three independent experiments.

    Article Snippet: Reverse-Transcription and Real-time quantitative PCR (qRT-PCR) RNA was extracted from the cells and reverse transcribed into cDNA using RNeasy® kit (Qiagen, Valencia, CA) and Omniscript RT Kit (Qiagen), respectively. mRNA levels were analyzed by qRT-PCR using SYBR green qRT-PCR Master Mix (Roche) on the LightCycler®480 System (Roche).

    Techniques: Expressing, Concentration Assay, Quantitative RT-PCR, Incubation, Standard Deviation

    PZ-235 suppresses liver expression of inflammatory cytokines in CCl 4 -treated mice receiving delayed pepducin treatment initiated at week 4. Livers were isolated from mice in at week 8, and quantitative PCR by SYBR Green (mean ± S.E., n

    Journal: The Journal of Biological Chemistry

    Article Title: Targeting Liver Fibrosis with a Cell-penetrating Protease-activated Receptor-2 (PAR2) Pepducin *

    doi: 10.1074/jbc.M116.732743

    Figure Lengend Snippet: PZ-235 suppresses liver expression of inflammatory cytokines in CCl 4 -treated mice receiving delayed pepducin treatment initiated at week 4. Livers were isolated from mice in at week 8, and quantitative PCR by SYBR Green (mean ± S.E., n

    Article Snippet: Total mRNA was extracted from the harvested liver samples using the RNeasy Mini kit (Qiagen). mRNA was reversed transcribed, and real time quantitative PCR was conducted using SYBR Green Master Mix (Roche Applied Science) and a 40-cycle thermocycling protocol.

    Techniques: Expressing, Mouse Assay, Isolation, Real-time Polymerase Chain Reaction, SYBR Green Assay

    YE, R5, and HC cells regulated the mRNA levels of cytokines-IL-10, IL-17A, IL-17F, and TNF-α-and transcription factors-Foxp3, and RORγ-in ConA-induced ALF mice. The regulatory effects of YE and R5 cells were significantly stronger than those of HC cells, with YE cells exhibiting the most optimal regulation. Quantitative PCR (qPCR) was used to detect the mRNA levels of IL-10 (A), IL-17A (B), IL-17F (C), TNF-α (D), Foxp3 (E), and RORγt (F) in the livers of Normal, ConA, YE, R5, and HC groups of mice. Data are presented as the mean ± SEM (n = 8; * P

    Journal: American Journal of Translational Research

    Article Title: Transplanted mouse liver stem cells at different stages of differentiation ameliorate concanavalin A-induced acute liver injury by modulating Tregs and Th17 cells in mice

    doi:

    Figure Lengend Snippet: YE, R5, and HC cells regulated the mRNA levels of cytokines-IL-10, IL-17A, IL-17F, and TNF-α-and transcription factors-Foxp3, and RORγ-in ConA-induced ALF mice. The regulatory effects of YE and R5 cells were significantly stronger than those of HC cells, with YE cells exhibiting the most optimal regulation. Quantitative PCR (qPCR) was used to detect the mRNA levels of IL-10 (A), IL-17A (B), IL-17F (C), TNF-α (D), Foxp3 (E), and RORγt (F) in the livers of Normal, ConA, YE, R5, and HC groups of mice. Data are presented as the mean ± SEM (n = 8; * P

    Article Snippet: The SYBR green quantitative PCR (qPCR) Master Mix (Annoron, Roche, Beijing, China) was used for qPCR in a LightCycler® 480 Real-Time PCR System (Annoron, Roche, Beijing, China).

    Techniques: Mouse Assay, Real-time Polymerase Chain Reaction