sybr green master mixes  (Thermo Fisher)


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    Name:
    SYBR Green PCR Master Mix
    Description:
    Alternative Product Try PowerUp SYBR Green Master Mix our newest high performance SYBR dye based master mix for superior performance at a very competitive price With PowerUp SYBR Green Master Mix we ve taken the best of SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis Everything you need for SYBR Green dye based PCR amplification and detection in a convenient single tube format Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye AmpliTaq Gold DNA Polymerase dNTPs with dUTP Passive Reference 1 and optimized buffer in the convenience of a single vial • Premixed components stored at 2 8°C significantly reduce assay setup time• SYBR Green I dye detects double stranded DNA so specific probes are not required• AmpliTaq Gold DNA polymerase minimizes nonspecific product formation to achieve superior performance• dUTP significantly reduces carryover contamination when used in conjunction with uracil DNA glycosylase• Proprietary buffer enhancements ensure performance and reliabilityMaximum Flexibility and ConvenienceApplied Biosystems SYBR Green PCR Master Mix provides maximum flexibility at reduced cost because no target specific TaqMan probes are required SYBR Green I dye is a double stranded DNA binding dye that detects any double stranded DNA generated during PCR The hot start enzyme AmpliTaq Gold DNA Polymerase minimizes nonspecific product formation including primer dimers yielding superior performance and sensitivity Passive Internal Reference 1 is provided to normalize non PCR related fluorescence fluctuations This minimizes well to well variability that can result from a variety of causes such as pipetting error or sample evaporation SYBR Green I dye is ideal for target identification screening assays or when a limited number of assays is needed
    Catalog Number:
    4309155
    Price:
    None
    Applications:
    Enzymes & Master Mixes for Real-Time PCR|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Gene Expression Analysis & Genotyping
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher sybr green master mixes
    Alternative Product Try PowerUp SYBR Green Master Mix our newest high performance SYBR dye based master mix for superior performance at a very competitive price With PowerUp SYBR Green Master Mix we ve taken the best of SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis Everything you need for SYBR Green dye based PCR amplification and detection in a convenient single tube format Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye AmpliTaq Gold DNA Polymerase dNTPs with dUTP Passive Reference 1 and optimized buffer in the convenience of a single vial • Premixed components stored at 2 8°C significantly reduce assay setup time• SYBR Green I dye detects double stranded DNA so specific probes are not required• AmpliTaq Gold DNA polymerase minimizes nonspecific product formation to achieve superior performance• dUTP significantly reduces carryover contamination when used in conjunction with uracil DNA glycosylase• Proprietary buffer enhancements ensure performance and reliabilityMaximum Flexibility and ConvenienceApplied Biosystems SYBR Green PCR Master Mix provides maximum flexibility at reduced cost because no target specific TaqMan probes are required SYBR Green I dye is a double stranded DNA binding dye that detects any double stranded DNA generated during PCR The hot start enzyme AmpliTaq Gold DNA Polymerase minimizes nonspecific product formation including primer dimers yielding superior performance and sensitivity Passive Internal Reference 1 is provided to normalize non PCR related fluorescence fluctuations This minimizes well to well variability that can result from a variety of causes such as pipetting error or sample evaporation SYBR Green I dye is ideal for target identification screening assays or when a limited number of assays is needed
    https://www.bioz.com/result/sybr green master mixes/product/Thermo Fisher
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    sybr green master mixes - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Quantification of Multiple Gene Expression in Individual Cells
    Article Snippet: .. Real-time quantitative PCR was performed by adding 10 μL of 2× SYBR Green PCR Master Mix (Applied Biosystems) to each well containing 4 μL of template and 6 μL of a primer mix with 0.25 μM of each specific primer (see Supplemental materials I and II) in a 20-μL reaction volume using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems). .. After a denaturation step at 95°C for 10 min, the cycle profile used was 30 sec at 95°C, 30 sec at 60°C, and 45 sec at 72°C for 60 cycles of amplification.

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed using SYBR Green PCR Master Mix (#4309155, Applied Biosystems, Foster City, CA) with 5 μl of immunoprecipitated material, 0.2 μM of primer [HIV-1 LTR (−69 − +175) Forward 5′-CTGGGCGGGACTGGGGAG-3′ and Reverse 5′-TCACACAACAGACGGGCACAC-3′]. .. The antibodies used for immunoprecipitation were as follows: total RNAP II CTD (ab817, Abcam), pS10-H3 (Novex, Life Technologies), BRCA1 (sc-642, Santa Cruz), p-BRCA1 S1423 (sc-101647, Santa, Cruz), Sp1 (5931, Cell Signaling), (IgG (sc-2027, Santa Cruz) or V5 (AbD Serotec, Oxford, UK).

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix Applied Biosystems). ..

    Article Title: Synergistic effects of snail and quercetin on renal cell carcinoma Caki-2 by altering AKT/mTOR/ERK1/2 signaling pathways
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix (Thermo, F-415XL) on Applied Biosystems 7300 Fast Real-Time PCR System. ..

    Amplification:

    Article Title: CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use
    Article Snippet: .. An aliquot of 5 μL of the RT reaction was amplified in duplicate in a final volume of 30 μL of SYBR Green PCR Master mix (Applied Biosystems, Foster City, CA, USA). .. The sample target RNA copy numbers were calculated using SDS 1.9 Software (Applied Biosystems, Foster City, CA, USA).

    Article Title: RNase L restricts the mobility of engineered retrotransposons in cultured human cells
    Article Snippet: .. The resultant cDNA was amplified using Sybr Green PCR master mix (Gibco/Life Technologies) on a StepOnePlus system according to manufacturer’s protocol. ..

    Sequencing:

    Article Title: Quantification of Multiple Gene Expression in Individual Cells
    Article Snippet: .. Real-time quantitative PCR was performed by adding 10 μL of 2× SYBR Green PCR Master Mix (Applied Biosystems) to each well containing 4 μL of template and 6 μL of a primer mix with 0.25 μM of each specific primer (see Supplemental materials I and II) in a 20-μL reaction volume using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems). .. After a denaturation step at 95°C for 10 min, the cycle profile used was 30 sec at 95°C, 30 sec at 60°C, and 45 sec at 72°C for 60 cycles of amplification.

    SYBR Green Assay:

    Article Title: Quantification of Multiple Gene Expression in Individual Cells
    Article Snippet: .. Real-time quantitative PCR was performed by adding 10 μL of 2× SYBR Green PCR Master Mix (Applied Biosystems) to each well containing 4 μL of template and 6 μL of a primer mix with 0.25 μM of each specific primer (see Supplemental materials I and II) in a 20-μL reaction volume using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems). .. After a denaturation step at 95°C for 10 min, the cycle profile used was 30 sec at 95°C, 30 sec at 60°C, and 45 sec at 72°C for 60 cycles of amplification.

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed using SYBR Green PCR Master Mix (#4309155, Applied Biosystems, Foster City, CA) with 5 μl of immunoprecipitated material, 0.2 μM of primer [HIV-1 LTR (−69 − +175) Forward 5′-CTGGGCGGGACTGGGGAG-3′ and Reverse 5′-TCACACAACAGACGGGCACAC-3′]. .. The antibodies used for immunoprecipitation were as follows: total RNAP II CTD (ab817, Abcam), pS10-H3 (Novex, Life Technologies), BRCA1 (sc-642, Santa Cruz), p-BRCA1 S1423 (sc-101647, Santa, Cruz), Sp1 (5931, Cell Signaling), (IgG (sc-2027, Santa Cruz) or V5 (AbD Serotec, Oxford, UK).

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix Applied Biosystems). ..

    Article Title: CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use
    Article Snippet: .. An aliquot of 5 μL of the RT reaction was amplified in duplicate in a final volume of 30 μL of SYBR Green PCR Master mix (Applied Biosystems, Foster City, CA, USA). .. The sample target RNA copy numbers were calculated using SDS 1.9 Software (Applied Biosystems, Foster City, CA, USA).

    Article Title: RNase L restricts the mobility of engineered retrotransposons in cultured human cells
    Article Snippet: .. The resultant cDNA was amplified using Sybr Green PCR master mix (Gibco/Life Technologies) on a StepOnePlus system according to manufacturer’s protocol. ..

    Article Title: Synergistic effects of snail and quercetin on renal cell carcinoma Caki-2 by altering AKT/mTOR/ERK1/2 signaling pathways
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix (Thermo, F-415XL) on Applied Biosystems 7300 Fast Real-Time PCR System. ..

    Article Title: A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes
    Article Snippet: .. PCR was performed using the SYBR GREEN PCR Master Mix from Applied Biosystems. .. The target-gene messenger RNA (mRNA) expression was normalized to the expression of GAPDH, and relative mRNA fold changes were calculated by the ΔΔCt method.

    Article Title: hCAF1/CNOT7 regulates interferon signalling by targeting STAT1
    Article Snippet: .. After DNA purification, qPCRs were performed in triplicate in a 96-well optical reaction plate using SYBR Green PCR Master Mix (Life Technology). .. The –ΔΔCt values for each locus were calculated with respect to the ChIP input DNA, normalized to a reference locus (3′ downstream region of the GAPDH gene).

    Immunoprecipitation:

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed using SYBR Green PCR Master Mix (#4309155, Applied Biosystems, Foster City, CA) with 5 μl of immunoprecipitated material, 0.2 μM of primer [HIV-1 LTR (−69 − +175) Forward 5′-CTGGGCGGGACTGGGGAG-3′ and Reverse 5′-TCACACAACAGACGGGCACAC-3′]. .. The antibodies used for immunoprecipitation were as follows: total RNAP II CTD (ab817, Abcam), pS10-H3 (Novex, Life Technologies), BRCA1 (sc-642, Santa Cruz), p-BRCA1 S1423 (sc-101647, Santa, Cruz), Sp1 (5931, Cell Signaling), (IgG (sc-2027, Santa Cruz) or V5 (AbD Serotec, Oxford, UK).

    DNA Purification:

    Article Title: hCAF1/CNOT7 regulates interferon signalling by targeting STAT1
    Article Snippet: .. After DNA purification, qPCRs were performed in triplicate in a 96-well optical reaction plate using SYBR Green PCR Master Mix (Life Technology). .. The –ΔΔCt values for each locus were calculated with respect to the ChIP input DNA, normalized to a reference locus (3′ downstream region of the GAPDH gene).

    Polymerase Chain Reaction:

    Article Title: Quantification of Multiple Gene Expression in Individual Cells
    Article Snippet: .. Real-time quantitative PCR was performed by adding 10 μL of 2× SYBR Green PCR Master Mix (Applied Biosystems) to each well containing 4 μL of template and 6 μL of a primer mix with 0.25 μM of each specific primer (see Supplemental materials I and II) in a 20-μL reaction volume using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems). .. After a denaturation step at 95°C for 10 min, the cycle profile used was 30 sec at 95°C, 30 sec at 60°C, and 45 sec at 72°C for 60 cycles of amplification.

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed using SYBR Green PCR Master Mix (#4309155, Applied Biosystems, Foster City, CA) with 5 μl of immunoprecipitated material, 0.2 μM of primer [HIV-1 LTR (−69 − +175) Forward 5′-CTGGGCGGGACTGGGGAG-3′ and Reverse 5′-TCACACAACAGACGGGCACAC-3′]. .. The antibodies used for immunoprecipitation were as follows: total RNAP II CTD (ab817, Abcam), pS10-H3 (Novex, Life Technologies), BRCA1 (sc-642, Santa Cruz), p-BRCA1 S1423 (sc-101647, Santa, Cruz), Sp1 (5931, Cell Signaling), (IgG (sc-2027, Santa Cruz) or V5 (AbD Serotec, Oxford, UK).

    Article Title: BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix Applied Biosystems). ..

    Article Title: CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use
    Article Snippet: .. An aliquot of 5 μL of the RT reaction was amplified in duplicate in a final volume of 30 μL of SYBR Green PCR Master mix (Applied Biosystems, Foster City, CA, USA). .. The sample target RNA copy numbers were calculated using SDS 1.9 Software (Applied Biosystems, Foster City, CA, USA).

    Article Title: RNase L restricts the mobility of engineered retrotransposons in cultured human cells
    Article Snippet: .. The resultant cDNA was amplified using Sybr Green PCR master mix (Gibco/Life Technologies) on a StepOnePlus system according to manufacturer’s protocol. ..

    Article Title: Synergistic effects of snail and quercetin on renal cell carcinoma Caki-2 by altering AKT/mTOR/ERK1/2 signaling pathways
    Article Snippet: .. Quantitative PCR was performed with SYBR Green PCR Master Mix (Thermo, F-415XL) on Applied Biosystems 7300 Fast Real-Time PCR System. ..

    Article Title: A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes
    Article Snippet: .. PCR was performed using the SYBR GREEN PCR Master Mix from Applied Biosystems. .. The target-gene messenger RNA (mRNA) expression was normalized to the expression of GAPDH, and relative mRNA fold changes were calculated by the ΔΔCt method.

    Article Title: hCAF1/CNOT7 regulates interferon signalling by targeting STAT1
    Article Snippet: .. After DNA purification, qPCRs were performed in triplicate in a 96-well optical reaction plate using SYBR Green PCR Master Mix (Life Technology). .. The –ΔΔCt values for each locus were calculated with respect to the ChIP input DNA, normalized to a reference locus (3′ downstream region of the GAPDH gene).

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  • 89
    Thermo Fisher sybr green master mixes
    Sybr Green Master Mixes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green master mixes/product/Thermo Fisher
    Average 89 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    sybr green master mixes - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

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