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Agilent technologies sureselect xt human all exon v5
Sureselect Xt Human All Exon V5, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sureselect xt human all exon v5/product/Agilent technologies
Average 92 stars, based on 18 article reviews
Price from $9.99 to $1999.99
sureselect xt human all exon v5 - by Bioz Stars, 2019-10
92/100 stars

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Amplification:

Article Title: TP53 and OSBPL10 alterations in diffuse large B-cell lymphoma: prognostic markers identified via exome analysis of cases with extreme prognosis
Article Snippet: Screening for gene mutations was performed via WES, using a customized capture probe set based on SureSelect XT Human All Exon V5 (Agilent, Santa Clara, USA). .. Screening for gene mutations was performed via WES, using a customized capture probe set based on SureSelect XT Human All Exon V5 (Agilent, Santa Clara, USA).

Article Title: Molecular Characterization of Colorectal Signet-Ring Cell Carcinoma Using Whole-Exome and RNA Sequencing
Article Snippet: Genomic DNA (1 μg) from each sample was sheared using the Covaris S220 (Covaris, Woburn, MA) and used to construct a library using SureSelect XT Human All Exon V5 and a SureSelect XT Reagent Kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. Genomic DNA (1 μg) from each sample was sheared using the Covaris S220 (Covaris, Woburn, MA) and used to construct a library using SureSelect XT Human All Exon V5 and a SureSelect XT Reagent Kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol.

Article Title: Nonlinear tumor evolution from dysplastic nodules to hepatocellular carcinoma
Article Snippet: Genomic DNA from 12 nodules and their corresponding cirrhotic tissues was sheared by the Covaris S220 (Covaris, Woburn, MA, USA) and used for the construction of a library using SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer's protocol. .. Genomic DNA from 12 nodules and their corresponding cirrhotic tissues was sheared by the Covaris S220 (Covaris, Woburn, MA, USA) and used for the construction of a library using SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer's protocol.

Article Title: Exome and transcriptome sequencing identifies loss of PDLIM2 in metastatic colorectal cancers
Article Snippet: Genomic DNA (1 µg) from each FFPE sample was sheared by Covaris S220 (Covaris, MA, USA) and used for construction of a library with SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. Genomic DNA (1 µg) from each FFPE sample was sheared by Covaris S220 (Covaris, MA, USA) and used for construction of a library with SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol.

Article Title: Genomic Characterization and Comparison of Multi-Regional and Pooled Tumor Biopsy Specimens
Article Snippet: Genomic DNA (1 μg) from each tissue sample was sheared using a Covaris S220 (Covaris, MA, USA) and used for the construction of a library using the SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. Genomic DNA (1 μg) from each tissue sample was sheared using a Covaris S220 (Covaris, MA, USA) and used for the construction of a library using the SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol.

Sample Prep:

Article Title: Whole exome sequencing identifies an AMBN missense mutation causing severe autosomal-dominant amelogenesis imperfecta and dentin disorders
Article Snippet: Three micrograms of genomic DNA isolated from each of three individuals (IV:1, IV:2, and IV:9) were processed according to the TruSeq DNA Sample Prep Kit protocol (Illumina, San Diego, CA, USA). .. Agilent SureSelect XT Human All Exon V5 (Agilent Technologies, Santa Clara, CA, USA) was used as the capture reagent.

Ligation:

Article Title: Molecular Characterization of Colorectal Signet-Ring Cell Carcinoma Using Whole-Exome and RNA Sequencing
Article Snippet: Genomic DNA (1 μg) from each sample was sheared using the Covaris S220 (Covaris, Woburn, MA) and used to construct a library using SureSelect XT Human All Exon V5 and a SureSelect XT Reagent Kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. After enriched exome libraries were multiplexed, the libraries were sequenced using a HiSeq 2500 sequencing platform (Illumina, San Diego, CA).

Article Title: Nonlinear tumor evolution from dysplastic nodules to hepatocellular carcinoma
Article Snippet: Genomic DNA from 12 nodules and their corresponding cirrhotic tissues was sheared by the Covaris S220 (Covaris, Woburn, MA, USA) and used for the construction of a library using SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer's protocol. .. After the enriched exome libraries were multiplexed, the libraries were sequenced on HiSeq 2500 sequencing platform (Illumina, San Diego, CA, USA).

Article Title: Exome and transcriptome sequencing identifies loss of PDLIM2 in metastatic colorectal cancers
Article Snippet: Genomic DNA (1 µg) from each FFPE sample was sheared by Covaris S220 (Covaris, MA, USA) and used for construction of a library with SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. After the enriched exome libraries were multiplexed, the libraries were sequenced on a HiSeq 2500 sequencing platform (Illumina, San Diego, CA, USA).

Article Title: Genomic Characterization and Comparison of Multi-Regional and Pooled Tumor Biopsy Specimens
Article Snippet: Genomic DNA (1 μg) from each tissue sample was sheared using a Covaris S220 (Covaris, MA, USA) and used for the construction of a library using the SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. After enriched exome libraries were multiplexed, the libraries were sequenced on the HiSeq 2500 sequencing platform (Illumina).

Mutagenesis:

Article Title: Analysis of Candidate Idarubicin Drug Resistance Genes in MOLT-3 Cells Using Exome Nuclear DNA
Article Snippet: Exomes of DNA samples were enriched using previously captured platforms obtained using the SureSelect XT Reagent Kit and SureSelect XT Human All Exon V5 (Agilent Technologies, Santa Clara, CA, USA) and assembled using the SeqNovaCS Data Analysis System at Hokkaido System Science Co., Ltd. (Sapporo, Japan). .. Exomes of DNA samples were enriched using previously captured platforms obtained using the SureSelect XT Reagent Kit and SureSelect XT Human All Exon V5 (Agilent Technologies, Santa Clara, CA, USA) and assembled using the SeqNovaCS Data Analysis System at Hokkaido System Science Co., Ltd. (Sapporo, Japan).

Isolation:

Article Title: Whole exome sequencing identifies an AMBN missense mutation causing severe autosomal-dominant amelogenesis imperfecta and dentin disorders
Article Snippet: Three micrograms of genomic DNA isolated from each of three individuals (IV:1, IV:2, and IV:9) were processed according to the TruSeq DNA Sample Prep Kit protocol (Illumina, San Diego, CA, USA). .. Agilent SureSelect XT Human All Exon V5 (Agilent Technologies, Santa Clara, CA, USA) was used as the capture reagent.

Article Title: Exome Sequencing Identification of EP300 Mutation in a Proband with Coloboma and Imperforate Anus: Possible Expansion of the Phenotypic Spectrum of Rubinstein-Taybi Syndrome
Article Snippet: Genomic DNA samples of the proband and her parents were isolated from peripheral blood using the NucleoSpin Blood Kit (Macherey-Nagel, Düren, Germany). .. Exome capture was performed with Agilent SureSelect XT Human All Exon V5 (Agilent, Santa Clara, Calif., USA).

Construct:

Article Title: Molecular Characterization of Colorectal Signet-Ring Cell Carcinoma Using Whole-Exome and RNA Sequencing
Article Snippet: For RNA, concentration and purity were measured using the NanoDrop and Bioanalyzer (Agilent Technologies). .. Genomic DNA (1 μg) from each sample was sheared using the Covaris S220 (Covaris, Woburn, MA) and used to construct a library using SureSelect XT Human All Exon V5 and a SureSelect XT Reagent Kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. This kit is designed to enrich 335,756 exons of 21,058 genes, covering ~71 Mb of the human genome.

Article Title: GREM1 and POLE variants in hereditary colorectal cancer syndromes
Article Snippet: The samples were then analyzed on the Bioanalyzer (Agilent Technology, Santa Clara, CA) for correct fragment sizes. .. Libraries were constructed using the “Agilent SureSelect XT Human All Exon 50Mb library kit” or “Agilent SureSelect_XT_Human_All_Exon_V5” for Illumina Paired End Sequencing (v3 protocol version 1.4.1 or G7530 90000_SureSelect_IlluminaXTMultiplexed_1_5) (Agilent Technologies) according to instructions. .. The concentration of each library was determined by use of the Qubit and the Bioanalyzer.

Purification:

Article Title: Molecular Characterization of Colorectal Signet-Ring Cell Carcinoma Using Whole-Exome and RNA Sequencing
Article Snippet: Genomic DNA (1 μg) from each sample was sheared using the Covaris S220 (Covaris, Woburn, MA) and used to construct a library using SureSelect XT Human All Exon V5 and a SureSelect XT Reagent Kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. Briefly, a paired-end DNA sequencing library was prepared by gDNA shearing, end-repair, A-tailing, paired-end adaptor ligation, and amplification.

Article Title: Nonlinear tumor evolution from dysplastic nodules to hepatocellular carcinoma
Article Snippet: Genomic DNA from 12 nodules and their corresponding cirrhotic tissues was sheared by the Covaris S220 (Covaris, Woburn, MA, USA) and used for the construction of a library using SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer's protocol. .. Briefly, a paired-end DNA sequencing library was prepared by genomic DNA shearing, end-repair, A-tailing, paired-end adaptor ligation, and amplification.

Article Title: Exome and transcriptome sequencing identifies loss of PDLIM2 in metastatic colorectal cancers
Article Snippet: Genomic DNA (1 µg) from each FFPE sample was sheared by Covaris S220 (Covaris, MA, USA) and used for construction of a library with SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. Briefly, a paired-end DNA sequencing library was prepared through gDNA shearing, end-repair, A-tailing, paired-end adaptor ligation, and amplification.

Article Title: Genomic Characterization and Comparison of Multi-Regional and Pooled Tumor Biopsy Specimens
Article Snippet: Genomic DNA (1 μg) from each tissue sample was sheared using a Covaris S220 (Covaris, MA, USA) and used for the construction of a library using the SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. Briefly, a paired-end DNA sequencing library was prepared through gDNA shearing, end-repair, A-tailing, paired-end adaptor ligation, and amplification.

Sequencing:

Article Title: Genetic Factors of the Disease Course after Sepsis: A Genome-Wide Study for 28 Day Mortality
Article Snippet: 2.2.3 For the exome sequencing study, 2–3 μg DNA per sample was fragmented on a Covaris M220 focused ultra-sonicator. .. Exomes were enriched using Agilent SureSelect XT Human All Exon V5 + UTRs kits targeting 74,856,280 bp in the coding sequence and untranslated regions of 20,791 genes. .. The mean depth of sequence coverage was 91-fold (range: 52- to 159-fold).

Article Title: TP53 and OSBPL10 alterations in diffuse large B-cell lymphoma: prognostic markers identified via exome analysis of cases with extreme prognosis
Article Snippet: Paragraph title: Sequencing analysis ... Screening for gene mutations was performed via WES, using a customized capture probe set based on SureSelect XT Human All Exon V5 (Agilent, Santa Clara, USA).

Article Title: Whole exome sequencing identifies an AMBN missense mutation causing severe autosomal-dominant amelogenesis imperfecta and dentin disorders
Article Snippet: Paragraph title: Whole exome sequencing ... Agilent SureSelect XT Human All Exon V5 (Agilent Technologies, Santa Clara, CA, USA) was used as the capture reagent.

Article Title: Concurrent somatic KRAS mutation and germline 10q22.3-q23.2 deletion in a patient with juvenile myelomonocytic leukemia, developmental delay, and multiple malformations: a case report
Article Snippet: Briefly, 3 μg DNA was sheared to create fragments of 150–200 bp in size. .. An adaptor-ligated library was prepared using the paired-end sequencing library prep kit (Agilent Technologies, Santa Clara, CA, USA) and both the coding exons and flanking intronic regions were enriched with SureSelect XT Human All Exon V5 (Agilent Technologies). .. Then, clusters were generated by isothermal bridge amplification with an Illumina cBot station, and sequencing was performed with an Illumina HiSeq 2500 System (Illumina, San Diego, CA, USA).

Article Title: Genetic Factors of the Disease Course After Sepsis: Rare Deleterious Variants Are Predictive
Article Snippet: These individuals (81/87.1% females; 12/12.9% males; median age: 66; quantiles Q1: 62, Q3: 69) are part of the population-based cohort POPGEN ( ) and their WES data were recently used as control group data in an early-onset IBD case-control study ( ). .. 2.2 2–3 μg genomic DNA per sepsis patient was fragmented on a Covaris M220 focused ultra-sonicator and exomes were enriched by use of Agilent SureSelect XT Human All Exon V5 + UTRs kit, targeting 74,856,280 bp encompassing the coding sequence and untranslated regions of 20,791 human genes. .. After sequence capture target enrichment, individual libraries were prepared which were quantified and checked for quality by Agilent High Sensitivity DNA chip.

Article Title: Comparison of exome-based HLA class I genotyping tools: identification of platform-specific genotyping errors
Article Snippet: PCR amplicon-based high-resolution typing on MiSeq (Illumina, San Diego, CA, USA) was performed for HLA-A , HLA-B and HLA-C in Scisco Genetics Inc. (Seattle, WA, USA). .. DNA libraries were prepared using SureSelect XT Human All Exon V5 (Agilent Technologies, Santa Clara, CA, USA) and whole-exome sequencing was performed by 100-bp paired-end reads on HiSeq2500 (Illumina) according to the manufacturers' protocol. .. To compare with PCR-based approaches, we selected a total of 961 samples across 13 different populations in the 1000 Genomes Project database ( http://www.1000genomes.org/ ) that had both exome data, which were sequenced by paired-end reads on Illumina sequencer (Genome Analyzer II or HiSeq 2000), and HLA type information experimentally determined by Sanger sequencing ( ).

Article Title: Molecular Characterization of Colorectal Signet-Ring Cell Carcinoma Using Whole-Exome and RNA Sequencing
Article Snippet: Paragraph title: Whole-Exome Sequencing ... Genomic DNA (1 μg) from each sample was sheared using the Covaris S220 (Covaris, Woburn, MA) and used to construct a library using SureSelect XT Human All Exon V5 and a SureSelect XT Reagent Kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol.

Article Title: GREM1 and POLE variants in hereditary colorectal cancer syndromes
Article Snippet: The samples were then analyzed on the Bioanalyzer (Agilent Technology, Santa Clara, CA) for correct fragment sizes. .. Libraries were constructed using the “Agilent SureSelect XT Human All Exon 50Mb library kit” or “Agilent SureSelect_XT_Human_All_Exon_V5” for Illumina Paired End Sequencing (v3 protocol version 1.4.1 or G7530 90000_SureSelect_IlluminaXTMultiplexed_1_5) (Agilent Technologies) according to instructions. .. The concentration of each library was determined by use of the Qubit and the Bioanalyzer.

Article Title: Exome Sequencing Identification of EP300 Mutation in a Proband with Coloboma and Imperforate Anus: Possible Expansion of the Phenotypic Spectrum of Rubinstein-Taybi Syndrome
Article Snippet: Paragraph title: Exome Sequencing ... Exome capture was performed with Agilent SureSelect XT Human All Exon V5 (Agilent, Santa Clara, Calif., USA).

Article Title: Nonlinear tumor evolution from dysplastic nodules to hepatocellular carcinoma
Article Snippet: Genomic DNA from 12 nodules and their corresponding cirrhotic tissues was sheared by the Covaris S220 (Covaris, Woburn, MA, USA) and used for the construction of a library using SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer's protocol. .. Genomic DNA from 12 nodules and their corresponding cirrhotic tissues was sheared by the Covaris S220 (Covaris, Woburn, MA, USA) and used for the construction of a library using SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer's protocol.

Article Title: Comprehensive genomic profiling of IgM multiple myeloma identifies IRF4 as a prognostic marker
Article Snippet: Paragraph title: Whole exome sequencing and data analysis ... Genomic DNA (1 μg) from the bone marrow and matching blood samples was sheared by Covaris S220 (Covaris, MA, USA) and used for library construction with SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer's protocols.

Article Title: Exome and transcriptome sequencing identifies loss of PDLIM2 in metastatic colorectal cancers
Article Snippet: Paragraph title: Exome sequencing ... Genomic DNA (1 µg) from each FFPE sample was sheared by Covaris S220 (Covaris, MA, USA) and used for construction of a library with SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol.

Article Title: Genomic Characterization and Comparison of Multi-Regional and Pooled Tumor Biopsy Specimens
Article Snippet: Paragraph title: Whole exome sequencing ... Genomic DNA (1 μg) from each tissue sample was sheared using a Covaris S220 (Covaris, MA, USA) and used for the construction of a library using the SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol.

Microarray:

Article Title: Concurrent somatic KRAS mutation and germline 10q22.3-q23.2 deletion in a patient with juvenile myelomonocytic leukemia, developmental delay, and multiple malformations: a case report
Article Snippet: Paragraph title: Whole-exome sequencing and chromosomal microarray analysis ... An adaptor-ligated library was prepared using the paired-end sequencing library prep kit (Agilent Technologies, Santa Clara, CA, USA) and both the coding exons and flanking intronic regions were enriched with SureSelect XT Human All Exon V5 (Agilent Technologies).

Generated:

Article Title: Genetic Factors of the Disease Course After Sepsis: Rare Deleterious Variants Are Predictive
Article Snippet: 2.2 2–3 μg genomic DNA per sepsis patient was fragmented on a Covaris M220 focused ultra-sonicator and exomes were enriched by use of Agilent SureSelect XT Human All Exon V5 + UTRs kit, targeting 74,856,280 bp encompassing the coding sequence and untranslated regions of 20,791 human genes. .. 2.2 2–3 μg genomic DNA per sepsis patient was fragmented on a Covaris M220 focused ultra-sonicator and exomes were enriched by use of Agilent SureSelect XT Human All Exon V5 + UTRs kit, targeting 74,856,280 bp encompassing the coding sequence and untranslated regions of 20,791 human genes.

Article Title: Preclinical Development of Bivalent Chimeric Antigen Receptors Targeting Both CD19 and CD22
Article Snippet: Poly-adenylated RNA libraries were generated and sequenced using TruSeq 4.0 chemistry on a HiSeq 2500 (Illumina) platform. .. Whole-exome data were generated using Agilent SureSelect XT Human All Exon V5 and TruSeq V4 chemistry and sequenced to a median of 100× coverage using the HiSeq 2500 (Illumina) platform. .. Whole-exome and RNA-sequencing data were mapped and analyzed using the CCR Collaborative Bioinformatics Resource (CCBR) pipeline.

Formalin-fixed Paraffin-Embedded:

Article Title: Nonlinear tumor evolution from dysplastic nodules to hepatocellular carcinoma
Article Snippet: Paragraph title: Genomic DNA preparation from FFPE tissues for WES ... Genomic DNA from 12 nodules and their corresponding cirrhotic tissues was sheared by the Covaris S220 (Covaris, Woburn, MA, USA) and used for the construction of a library using SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer's protocol.

Article Title: Exome and transcriptome sequencing identifies loss of PDLIM2 in metastatic colorectal cancers
Article Snippet: To improve sequencing quality, we used strict sample quality thresholds for extracted DNA: 1) purity: 260/280 > 1.8, 260/230 > 1.8; 2) total amount > 250 ng; and 3) degradation: ΔCt value < 2.0 or DNA median size > 0.35 kb. .. Genomic DNA (1 µg) from each FFPE sample was sheared by Covaris S220 (Covaris, MA, USA) and used for construction of a library with SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. This kit is designed to enrich 335,756 exons of 21,058 genes, covering ~71 Mb of the human genome.

DNA Sequencing:

Article Title: Molecular Characterization of Colorectal Signet-Ring Cell Carcinoma Using Whole-Exome and RNA Sequencing
Article Snippet: Genomic DNA (1 μg) from each sample was sheared using the Covaris S220 (Covaris, Woburn, MA) and used to construct a library using SureSelect XT Human All Exon V5 and a SureSelect XT Reagent Kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. After enriched exome libraries were multiplexed, the libraries were sequenced using a HiSeq 2500 sequencing platform (Illumina, San Diego, CA).

Article Title: Nonlinear tumor evolution from dysplastic nodules to hepatocellular carcinoma
Article Snippet: Genomic DNA from 12 nodules and their corresponding cirrhotic tissues was sheared by the Covaris S220 (Covaris, Woburn, MA, USA) and used for the construction of a library using SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer's protocol. .. After the enriched exome libraries were multiplexed, the libraries were sequenced on HiSeq 2500 sequencing platform (Illumina, San Diego, CA, USA).

Article Title: Exome and transcriptome sequencing identifies loss of PDLIM2 in metastatic colorectal cancers
Article Snippet: Genomic DNA (1 µg) from each FFPE sample was sheared by Covaris S220 (Covaris, MA, USA) and used for construction of a library with SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. After the enriched exome libraries were multiplexed, the libraries were sequenced on a HiSeq 2500 sequencing platform (Illumina, San Diego, CA, USA).

Article Title: Genomic Characterization and Comparison of Multi-Regional and Pooled Tumor Biopsy Specimens
Article Snippet: Genomic DNA (1 μg) from each tissue sample was sheared using a Covaris S220 (Covaris, MA, USA) and used for the construction of a library using the SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. After enriched exome libraries were multiplexed, the libraries were sequenced on the HiSeq 2500 sequencing platform (Illumina).

Polymerase Chain Reaction:

Article Title: TP53 and OSBPL10 alterations in diffuse large B-cell lymphoma: prognostic markers identified via exome analysis of cases with extreme prognosis
Article Snippet: Screening for gene mutations was performed via WES, using a customized capture probe set based on SureSelect XT Human All Exon V5 (Agilent, Santa Clara, USA). .. Screening for gene mutations was performed via WES, using a customized capture probe set based on SureSelect XT Human All Exon V5 (Agilent, Santa Clara, USA).

Article Title: Comprehensive genomic profiling of IgM multiple myeloma identifies IRF4 as a prognostic marker
Article Snippet: Genomic DNA (1 μg) from the bone marrow and matching blood samples was sheared by Covaris S220 (Covaris, MA, USA) and used for library construction with SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer's protocols. .. Sequencing reads were aligned to the UCSC hg19 reference genome (downloaded from http://genome.ucsc.edu ) using Burrows-Wheeler Aligner (BWA) [ ], version0.6.2 with default settings.

Staining:

Article Title: Nonlinear tumor evolution from dysplastic nodules to hepatocellular carcinoma
Article Snippet: Ten formalin fixed paraffin embedded (FFPE) sections of 10 μm from each lesion were stained with eosin. .. Genomic DNA from 12 nodules and their corresponding cirrhotic tissues was sheared by the Covaris S220 (Covaris, Woburn, MA, USA) and used for the construction of a library using SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer's protocol.

Variant Assay:

Article Title: Whole exome sequencing identifies an AMBN missense mutation causing severe autosomal-dominant amelogenesis imperfecta and dentin disorders
Article Snippet: Agilent SureSelect XT Human All Exon V5 (Agilent Technologies, Santa Clara, CA, USA) was used as the capture reagent. .. Agilent SureSelect XT Human All Exon V5 (Agilent Technologies, Santa Clara, CA, USA) was used as the capture reagent.

Article Title: Concurrent somatic KRAS mutation and germline 10q22.3-q23.2 deletion in a patient with juvenile myelomonocytic leukemia, developmental delay, and multiple malformations: a case report
Article Snippet: An adaptor-ligated library was prepared using the paired-end sequencing library prep kit (Agilent Technologies, Santa Clara, CA, USA) and both the coding exons and flanking intronic regions were enriched with SureSelect XT Human All Exon V5 (Agilent Technologies). .. An adaptor-ligated library was prepared using the paired-end sequencing library prep kit (Agilent Technologies, Santa Clara, CA, USA) and both the coding exons and flanking intronic regions were enriched with SureSelect XT Human All Exon V5 (Agilent Technologies).

Article Title: Preclinical Development of Bivalent Chimeric Antigen Receptors Targeting Both CD19 and CD22
Article Snippet: Whole-exome data were generated using Agilent SureSelect XT Human All Exon V5 and TruSeq V4 chemistry and sequenced to a median of 100× coverage using the HiSeq 2500 (Illumina) platform. .. Whole-exome data were generated using Agilent SureSelect XT Human All Exon V5 and TruSeq V4 chemistry and sequenced to a median of 100× coverage using the HiSeq 2500 (Illumina) platform.

Software:

Article Title: Whole exome sequencing identifies an AMBN missense mutation causing severe autosomal-dominant amelogenesis imperfecta and dentin disorders
Article Snippet: Agilent SureSelect XT Human All Exon V5 (Agilent Technologies, Santa Clara, CA, USA) was used as the capture reagent. .. The read alignment and variant calling were performed by the BaseSpace BWA Enrichment v1.0 App.

Hybridization:

Article Title: Molecular Characterization of Colorectal Signet-Ring Cell Carcinoma Using Whole-Exome and RNA Sequencing
Article Snippet: Genomic DNA (1 μg) from each sample was sheared using the Covaris S220 (Covaris, Woburn, MA) and used to construct a library using SureSelect XT Human All Exon V5 and a SureSelect XT Reagent Kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. Briefly, a paired-end DNA sequencing library was prepared by gDNA shearing, end-repair, A-tailing, paired-end adaptor ligation, and amplification.

Article Title: Nonlinear tumor evolution from dysplastic nodules to hepatocellular carcinoma
Article Snippet: Genomic DNA from 12 nodules and their corresponding cirrhotic tissues was sheared by the Covaris S220 (Covaris, Woburn, MA, USA) and used for the construction of a library using SureSelect XT Human All Exon v5 and SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer's protocol. .. Briefly, a paired-end DNA sequencing library was prepared by genomic DNA shearing, end-repair, A-tailing, paired-end adaptor ligation, and amplification.

Article Title: Exome and transcriptome sequencing identifies loss of PDLIM2 in metastatic colorectal cancers
Article Snippet: Genomic DNA (1 µg) from each FFPE sample was sheared by Covaris S220 (Covaris, MA, USA) and used for construction of a library with SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. Briefly, a paired-end DNA sequencing library was prepared through gDNA shearing, end-repair, A-tailing, paired-end adaptor ligation, and amplification.

Article Title: Genomic Characterization and Comparison of Multi-Regional and Pooled Tumor Biopsy Specimens
Article Snippet: Genomic DNA (1 μg) from each tissue sample was sheared using a Covaris S220 (Covaris, MA, USA) and used for the construction of a library using the SureSelect XT Human All Exon v5 and the SureSelect XT reagent kit, HSQ (Agilent Technologies) according to the manufacturer’s protocol. .. Briefly, a paired-end DNA sequencing library was prepared through gDNA shearing, end-repair, A-tailing, paired-end adaptor ligation, and amplification.

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    Agilent technologies sureselect xt human all exon v5 kit
    Sureselect Xt Human All Exon V5 Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sureselect xt human all exon v5 kit/product/Agilent technologies
    Average 92 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    sureselect xt human all exon v5 kit - by Bioz Stars, 2019-10
    92/100 stars
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