supersignal west pico chemiluminescent substrate  (Thermo Fisher)


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    Structured Review

    Thermo Fisher supersignal west pico chemiluminescent substrate
    Supersignal West Pico Chemiluminescent Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/supersignal west pico chemiluminescent substrate/product/Thermo Fisher
    Average 99 stars, based on 99 article reviews
    Price from $9.99 to $1999.99
    supersignal west pico chemiluminescent substrate - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: The p75 Neurotrophin Receptor Is an Essential Mediator of Impairments in Hippocampal-Dependent Associative Plasticity and Memory Induced by Sleep Deprivation
    Article Snippet: The tissues were then lysed in Tissue Protein Extraction reagent (T-PER; ThermoFisher Scientific) with protease and phosphatase inhibitor (ThermoFisher Scientific), followed by centrifugation for 5 min at 10,000 rpm at 4°C. .. Signals were generated by using the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific).

    Article Title: Contemporary Circulating Enterovirus D68 Strains Infect and Undergo Retrograde Axonal Transport in Spinal Motor Neurons Independent of Sialic Acid
    Article Snippet: For all samples, the cell debris was pelleted by centrifugation at 20,800 × g at 4°C for 20 min. .. Following five washes in TBST, the blots were incubated in secondary antibody in 5% dry milk in TBST for 1 h. After five washes in TBST, the blots were developed using SuperSignal West Pico chemiluminescent substrate (catalog number 34580; Thermo Fisher) and imaged using a FluorochemQ MultiImage III workstation.

    Blocking Assay:

    Article Title: Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice.
    Article Snippet: The protein extracts were separated by SDS-PAGE, transferred to Hybond-ECL nitrocellulose membrane (GE Healthcare, Chalfont St. Giles, United Kingdom), which was incubated with a blocking buffer (0.1% Tween and 5% non-fat dry milk in PBS), and then with anti-N and anti-P polyclonal antibodies previously obtained (Simabuco et al., 2007) . .. After three washes with washing solution, the membrane was treated with SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology, Rockford, USA), exposed to Hyperfilm (Amersham) and visualized.

    Article Title: The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology
    Article Snippet: Membranes were washed in blocking buffer lacking milk three times before the addition of horseradish peroxidase-conjugated secondary antibodies for 45 min at room temperature. .. Membranes were once again washed three times before development using SuperSignal West Pico Chemiluminescent substrate (ThermoFisher) and imaging using a LAS-3000 imager (Fujifilm).

    Article Title: Contemporary Circulating Enterovirus D68 Strains Infect and Undergo Retrograde Axonal Transport in Spinal Motor Neurons Independent of Sialic Acid
    Article Snippet: The proteins were transferred onto polyvinylidene difluoride (PVDF) blots and blocked with 5% dry milk powder in 1% Tris-buffered saline–Tween 20 (TBST) for 2 h. After blocking, the blots were incubated in primary antibody in 5% dry milk in TBST overnight at 4°C. .. Following five washes in TBST, the blots were incubated in secondary antibody in 5% dry milk in TBST for 1 h. After five washes in TBST, the blots were developed using SuperSignal West Pico chemiluminescent substrate (catalog number 34580; Thermo Fisher) and imaged using a FluorochemQ MultiImage III workstation.

    Article Title: Perturbation of base excision repair sensitizes breast cancer cells to APOBEC3 deaminase-mediated mutations
    Article Snippet: After blocking with 3% BSA (in TBST: 50 mM Tris-Cl, pH 7.5, 150 mM NaCl, and 0.06% Tween-20), membranes were probed with primary antibodies at 4°C overnight. .. The membranes were washed three times in TBST (with 0.06% Tween-20), incubated with anti-Rabbit or anti-Mouse IgG-Peroxidase at RT for 1.5 hr, then treated for 5 min with SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific, 34580) and exposed to X-ray film.

    Article Title: Artificial miRNAs Reduce Human Mutant Huntingtin Throughout the Striatum in a Transgenic Sheep Model of Huntington's Disease
    Article Snippet: .. Blots were washed in TBST, incubated in peroxidase labeled secondary antibodies (Jackson Immunoresearch), diluted 1:5,000 in blocking solution for 1 h at room temperature, washed in TBST, and incubated in SuperSignal West Pico Chemiluminescent Substrate (Pierce #34080). .. Images were obtained with a CCD imaging system (Alpha Innotech) and Hyperfilm ECL (GE Healthcare).

    Electrophoresis:

    Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
    Article Snippet: Equal aliquots (50 μg) of total protein for each sample were denatured with SDS-PAGE sample buffer and separated by electrophoresis on an SDS polyacrylamide gel. .. SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific Pierce, Rockford, IL) was used for detection, and photographic emulsion was used to identify the protein bands, which were subsequently quantified by densitometry.

    Incubation:

    Article Title: phlda3 overexpression impairs specification of hemangioblasts and vascular development
    Article Snippet: .. Signals were detected by incubation with a SuperSignal West Pico Chemiluminescent Substrate (Thermo) and ChemiDoc XRS (Bio-Rad). ..

    Article Title: Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice.
    Article Snippet: The membrane was washed three times with washing solution (Tween 0.1% in PBS) and incubated with an anti-mouse peroxidaseconjugated antibody (KPL, Washington, USA). .. After three washes with washing solution, the membrane was treated with SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology, Rockford, USA), exposed to Hyperfilm (Amersham) and visualized.

    Article Title: The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology
    Article Snippet: For immunoblot analysis, protein samples were transferred to 0.2 μm nitrocellulose membrane (Amersham Biosciences) after incubation in transfer buffer (10% (v/v) Tris-glycine-SDS buffer, 20% (v/v) ethanol) using a TransBlot SD semi-dry transfer cell (BioRad) at 24 V and 0.18 A for 44 min. Membranes were blocked using a solution of 5% (w/v) skimmed milk powder (Sigma), 50 mM Tris-HCl pH 8.0, 150 mM NaCl and 0.1% (v/v) Tween-20 for 1 h before the addition of primary antibodies. .. Membranes were once again washed three times before development using SuperSignal West Pico Chemiluminescent substrate (ThermoFisher) and imaging using a LAS-3000 imager (Fujifilm).

    Article Title: The p75 Neurotrophin Receptor Is an Essential Mediator of Impairments in Hippocampal-Dependent Associative Plasticity and Memory Induced by Sleep Deprivation
    Article Snippet: The gels were then transferred to PVDF membranes (Bio-Rad) in a semidry transfer cell (Bio-Rad) for 1 h at 15 V. Membranes were incubated with 5% w/v dry milk or bovine serum albumin in 1× TBST and immunoblotted with primary antibodies against phosphodiesterase (PDE4A5; 1:500; Abcam), p75NTR (1:1000, Neuromics), total or phospho-ERK (1:1000; Cell Signaling Technology), total or phospho-CREB (1:1000; Cell Signaling Technology), RhoA (1:1000, Abcam), ROCK2 (1:1000; Cell Signaling Technology), total LIMK1 (1:1000; Cell Signaling Technology), phospho-LIMK1 (1:1000; Abcam), total or phospho-cofilin (1:1000; Cell Signaling Technology), and tubulin (1:20,000; Sigma-Aldrich) followed by secondary peroxidase-conjugated antibodies directed against mouse (tubulin), rabbit (ERK, CREB, RhoA, ROCK2, LIMK1, and cofilin), and goat (p75NTR ). .. Signals were generated by using the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific).

    Article Title: Contemporary Circulating Enterovirus D68 Strains Infect and Undergo Retrograde Axonal Transport in Spinal Motor Neurons Independent of Sialic Acid
    Article Snippet: .. Following five washes in TBST, the blots were incubated in secondary antibody in 5% dry milk in TBST for 1 h. After five washes in TBST, the blots were developed using SuperSignal West Pico chemiluminescent substrate (catalog number 34580; Thermo Fisher) and imaged using a FluorochemQ MultiImage III workstation. .. Images were brightened linearly and equally using FluorochemQ image software.

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: Membrane was blocked with 5% dry milk in 1× Tris-buffered saline (TBS) buffer for 40 min, washed twice with washing solution (TBS + 0.06% Tween 20), and incubated with anti-ESAT-6 rabbit polyclonal antibody (Cat. No. PA1-19446, Thermo Fisher Sci., Waltham, MA, USA) for 50 min. Three washes (TBS + 0.06% Tween 20) were made followed by incubation with HRP-conjugated anti-rabbit IgG as the secondary antibody (Cat. No. A0545, Merck-Sigma-Aldrich, St. Louis, MI, USA) for 50 min. .. The bands were detected by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA).

    Article Title: Perturbation of base excision repair sensitizes breast cancer cells to APOBEC3 deaminase-mediated mutations
    Article Snippet: .. The membranes were washed three times in TBST (with 0.06% Tween-20), incubated with anti-Rabbit or anti-Mouse IgG-Peroxidase at RT for 1.5 hr, then treated for 5 min with SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific, 34580) and exposed to X-ray film. .. Immunofluorescence Hs578T cells seeded in 6-well plates were transfected with 1.5 μg phAPOBEC3B-HA (A3B-3HA) plasmid, and 24 hr post-transfection, the cells were seeded into Lab-Tek II Chamber Slide System (Nunc, 177380).

    Article Title: Long non-coding RNA PSMB8-AS1 regulates influenza virus replication
    Article Snippet: Membranes were washed with TRIS-buffered saline for 5 min × 3 times and incubated with species-appropriate horseradish peroxidase-labeled secondary antibody for 1 h at room temperature. .. Finally, the membranes were developed using SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Fischer, Waltham, MA, USA).

    Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
    Article Snippet: The membranes were washed with TBS containing 0.1%Tween-20 wash buffer after each antibody incubation cycle. .. SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific Pierce, Rockford, IL) was used for detection, and photographic emulsion was used to identify the protein bands, which were subsequently quantified by densitometry.

    Article Title: A Matricryptic Conformation of the Integrin-Binding Domain of Fibronectin Regulates Platelet-Derived Growth Factor-Induced Intracellular Calcium Release
    Article Snippet: .. Blots were washed with TBS-T, incubated with goat anti-rabbit (1:5000) or goat anti-mouse (1:20,000) horseradish peroxidase-linked secondary antibodies, and developed using SuperSignal® West Pico Chemiluminescent Substrate (Thermo Fisher Scientific). .. The average net band intensity for duplicate samples was determined by densitometry using Carestream Molecular Imaging Software (Rochester, NY, USA).

    Article Title: Artificial miRNAs Reduce Human Mutant Huntingtin Throughout the Striatum in a Transgenic Sheep Model of Huntington's Disease
    Article Snippet: .. Blots were washed in TBST, incubated in peroxidase labeled secondary antibodies (Jackson Immunoresearch), diluted 1:5,000 in blocking solution for 1 h at room temperature, washed in TBST, and incubated in SuperSignal West Pico Chemiluminescent Substrate (Pierce #34080). .. Images were obtained with a CCD imaging system (Alpha Innotech) and Hyperfilm ECL (GE Healthcare).

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: First, each membrane was blocked (5% dry milk in TBS 1× for 40 min) at room temperature, washed twice with TBS + 0.06% Tween 20, and incubated with anti-DnaK mouse polyclonal antibody (Cat. No. ADI-SPA-880, Enzo Life Sciences, Farmingdale, NY, USA). .. The immunoreactive proteins were revealed by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA).

    Stripping Membranes:

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: The bands were detected by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA). .. A home-made luminescent strip put on top of PVDF membrane for immunoreactive protein detection was used and arranged in the same place as the SDS-PAGE molecular weight marker.

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: The immunoreactive proteins were revealed by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA). .. A home-made luminescent strip put on top of PVDF membrane for immunoreactive protein detection was used and arranged in the same place as the SDS-PAGE molecular weight marker.

    Expressing:

    Article Title: An inter‐dimer allosteric switch controls NMDA receptor activity
    Article Snippet: Oocytes expressing “silent” mutant receptors were selected based on redox treatment of other oocytes of the same batch revealing high expression of “silent” receptors. .. Protein bands were visualized using secondary goat peroxidase‐linked anti‐mouse antibodies (1:10,000, Jackson ImmunoResearch catalog number #115‐035‐003), with the SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Scientific™).

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: Paragraph title: Analysis of recombinant protein expression by SDS-PAGE and Western blot ... The bands were detected by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA).

    BIA-KA:

    Article Title: Contemporary Circulating Enterovirus D68 Strains Infect and Undergo Retrograde Axonal Transport in Spinal Motor Neurons Independent of Sialic Acid
    Article Snippet: The supernatant was collected, and the final protein concentration was determined using a bicinchoninic acid (BCA) assay (catalog number 23225; Thermo Fisher). .. Following five washes in TBST, the blots were incubated in secondary antibody in 5% dry milk in TBST for 1 h. After five washes in TBST, the blots were developed using SuperSignal West Pico chemiluminescent substrate (catalog number 34580; Thermo Fisher) and imaged using a FluorochemQ MultiImage III workstation.

    Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
    Article Snippet: The concentration of protein was determined using the BCA Protein Assay Kit (Thermo Scientific Pierce, Rockford, IL). .. SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific Pierce, Rockford, IL) was used for detection, and photographic emulsion was used to identify the protein bands, which were subsequently quantified by densitometry.

    Western Blot:

    Article Title: phlda3 overexpression impairs specification of hemangioblasts and vascular development
    Article Snippet: Paragraph title: Western blot analysis ... Signals were detected by incubation with a SuperSignal West Pico Chemiluminescent Substrate (Thermo) and ChemiDoc XRS (Bio-Rad).

    Article Title: Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice.
    Article Snippet: Paragraph title: Western blot ... After three washes with washing solution, the membrane was treated with SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology, Rockford, USA), exposed to Hyperfilm (Amersham) and visualized.

    Article Title: An inter‐dimer allosteric switch controls NMDA receptor activity
    Article Snippet: Supernatants enriched in membrane proteins were collected and separated in two equal volumes for subsequent Western blotting experiments in non‐reducing and reducing (9% vol/vol β‐mercaptoethanol added in the loading buffer) conditions. .. Protein bands were visualized using secondary goat peroxidase‐linked anti‐mouse antibodies (1:10,000, Jackson ImmunoResearch catalog number #115‐035‐003), with the SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Scientific™).

    Article Title: The p75 Neurotrophin Receptor Is an Essential Mediator of Impairments in Hippocampal-Dependent Associative Plasticity and Memory Induced by Sleep Deprivation
    Article Snippet: Paragraph title: Western blotting. ... Signals were generated by using the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific).

    Article Title: Contemporary Circulating Enterovirus D68 Strains Infect and Undergo Retrograde Axonal Transport in Spinal Motor Neurons Independent of Sialic Acid
    Article Snippet: Paragraph title: Western blotting. ... Following five washes in TBST, the blots were incubated in secondary antibody in 5% dry milk in TBST for 1 h. After five washes in TBST, the blots were developed using SuperSignal West Pico chemiluminescent substrate (catalog number 34580; Thermo Fisher) and imaged using a FluorochemQ MultiImage III workstation.

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: Paragraph title: Analysis of recombinant protein expression by SDS-PAGE and Western blot ... The bands were detected by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA).

    Article Title: Perturbation of base excision repair sensitizes breast cancer cells to APOBEC3 deaminase-mediated mutations
    Article Snippet: Paragraph title: Western blotting ... The membranes were washed three times in TBST (with 0.06% Tween-20), incubated with anti-Rabbit or anti-Mouse IgG-Peroxidase at RT for 1.5 hr, then treated for 5 min with SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific, 34580) and exposed to X-ray film.

    Article Title: Long non-coding RNA PSMB8-AS1 regulates influenza virus replication
    Article Snippet: Paragraph title: Western blot analysis ... Finally, the membranes were developed using SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Fischer, Waltham, MA, USA).

    Article Title: A Matricryptic Conformation of the Integrin-Binding Domain of Fibronectin Regulates Platelet-Derived Growth Factor-Induced Intracellular Calcium Release
    Article Snippet: Immunoblots were blocked with 3% BSA (Heat-Shock Fraction V, pH = 7.5; Affymetrix, Cleveland, OH, USA) in Tris-buffered saline with Tween 20 (TBS-T) (20 mM Tris-HCl pH 7.6, 137 mM NaCl, 1% Tween 20) and incubated with anti-vinculin mAb (1:2000) or anti-phospho-PI3K pAb (1:2000) overnight at 4 °C. .. Blots were washed with TBS-T, incubated with goat anti-rabbit (1:5000) or goat anti-mouse (1:20,000) horseradish peroxidase-linked secondary antibodies, and developed using SuperSignal® West Pico Chemiluminescent Substrate (Thermo Fisher Scientific).

    Article Title: Artificial miRNAs Reduce Human Mutant Huntingtin Throughout the Striatum in a Transgenic Sheep Model of Huntington's Disease
    Article Snippet: Paragraph title: Western blotting ... Blots were washed in TBST, incubated in peroxidase labeled secondary antibodies (Jackson Immunoresearch), diluted 1:5,000 in blocking solution for 1 h at room temperature, washed in TBST, and incubated in SuperSignal West Pico Chemiluminescent Substrate (Pierce #34080).

    Transfection:

    Article Title: Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice.
    Article Snippet: Western blot After DNA transfection or HRSV infection, cells were washed with phosphate buffered saline (PBS), scraped and lysed with a SDS sample buffer (50 mM Tris-HCl, pH 6.8; 10 mM EDTA; 10% glycerol; 0.2% bromphenol blue; 2% SDS; 1% ␤-mercaptoethanol). .. After three washes with washing solution, the membrane was treated with SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology, Rockford, USA), exposed to Hyperfilm (Amersham) and visualized.

    Protease Inhibitor:

    Article Title: An inter‐dimer allosteric switch controls NMDA receptor activity
    Article Snippet: Each mix of 16 oocytes was then homogenized on ice by back and forth pipetting with 160 μl of lysis buffer (20 mM Tris pH 8.0, 50 mM NaCl, 1% DDM, 1/20 of a complete protease inhibitor cocktail tablet, Roche Complete, Mini) until a homogenous suspension was obtained. .. Protein bands were visualized using secondary goat peroxidase‐linked anti‐mouse antibodies (1:10,000, Jackson ImmunoResearch catalog number #115‐035‐003), with the SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Scientific™).

    Article Title: Perturbation of base excision repair sensitizes breast cancer cells to APOBEC3 deaminase-mediated mutations
    Article Snippet: Western blotting Cells were harvested and centrifuged at 2,000 ×g for 2 min. For nuclear proteins, cells were lysed in RIPA buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% NP-40, 1% Sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitor cocktail (Roche, 04693159001), and centrifuged at 13,000 ×g for 10 min at 4°C. .. The membranes were washed three times in TBST (with 0.06% Tween-20), incubated with anti-Rabbit or anti-Mouse IgG-Peroxidase at RT for 1.5 hr, then treated for 5 min with SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific, 34580) and exposed to X-ray film.

    Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
    Article Snippet: Liquid nitrogen flash-frozen lung tissue samples and fetal lung fibroblasts following treatment conditions were suspended in radioimmunoprecipitation assay buffer containing 1 mM EDTA and EGTA (Boston BioProducts, Ashland, MA) supplemented with 1 mM PMSF and a complete protease inhibitor mixture (Roche Diagnostics, Indianapolis, IN), sonicated, and centrifuged at 4°C for 10 min at 14,000 rpm. .. SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific Pierce, Rockford, IL) was used for detection, and photographic emulsion was used to identify the protein bands, which were subsequently quantified by densitometry.

    Infection:

    Article Title: Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice.
    Article Snippet: Western blot After DNA transfection or HRSV infection, cells were washed with phosphate buffered saline (PBS), scraped and lysed with a SDS sample buffer (50 mM Tris-HCl, pH 6.8; 10 mM EDTA; 10% glycerol; 0.2% bromphenol blue; 2% SDS; 1% ␤-mercaptoethanol). .. After three washes with washing solution, the membrane was treated with SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology, Rockford, USA), exposed to Hyperfilm (Amersham) and visualized.

    Generated:

    Article Title: The p75 Neurotrophin Receptor Is an Essential Mediator of Impairments in Hippocampal-Dependent Associative Plasticity and Memory Induced by Sleep Deprivation
    Article Snippet: .. Signals were generated by using the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific). .. The amount of protein was quantified by densitometric measurement of Western blots using ImageJ (NIH software).

    Imaging:

    Article Title: The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology
    Article Snippet: .. Membranes were once again washed three times before development using SuperSignal West Pico Chemiluminescent substrate (ThermoFisher) and imaging using a LAS-3000 imager (Fujifilm). .. Protein purification Purification of proteins was performed as described previously ( ).

    Article Title: A Matricryptic Conformation of the Integrin-Binding Domain of Fibronectin Regulates Platelet-Derived Growth Factor-Induced Intracellular Calcium Release
    Article Snippet: Blots were washed with TBS-T, incubated with goat anti-rabbit (1:5000) or goat anti-mouse (1:20,000) horseradish peroxidase-linked secondary antibodies, and developed using SuperSignal® West Pico Chemiluminescent Substrate (Thermo Fisher Scientific). .. The average net band intensity for duplicate samples was determined by densitometry using Carestream Molecular Imaging Software (Rochester, NY, USA).

    Article Title: Artificial miRNAs Reduce Human Mutant Huntingtin Throughout the Striatum in a Transgenic Sheep Model of Huntington's Disease
    Article Snippet: Blots were washed in TBST, incubated in peroxidase labeled secondary antibodies (Jackson Immunoresearch), diluted 1:5,000 in blocking solution for 1 h at room temperature, washed in TBST, and incubated in SuperSignal West Pico Chemiluminescent Substrate (Pierce #34080). .. Images were obtained with a CCD imaging system (Alpha Innotech) and Hyperfilm ECL (GE Healthcare).

    Protein Concentration:

    Article Title: The p75 Neurotrophin Receptor Is an Essential Mediator of Impairments in Hippocampal-Dependent Associative Plasticity and Memory Induced by Sleep Deprivation
    Article Snippet: The appropriate protein concentration was determined and the protein was added to the sample buffer, heated at 95°C for 10 min before loading onto SDS-polyacrylamide gels. .. Signals were generated by using the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific).

    Article Title: Contemporary Circulating Enterovirus D68 Strains Infect and Undergo Retrograde Axonal Transport in Spinal Motor Neurons Independent of Sialic Acid
    Article Snippet: The supernatant was collected, and the final protein concentration was determined using a bicinchoninic acid (BCA) assay (catalog number 23225; Thermo Fisher). .. Following five washes in TBST, the blots were incubated in secondary antibody in 5% dry milk in TBST for 1 h. After five washes in TBST, the blots were developed using SuperSignal West Pico chemiluminescent substrate (catalog number 34580; Thermo Fisher) and imaged using a FluorochemQ MultiImage III workstation.

    Sonication:

    Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
    Article Snippet: Liquid nitrogen flash-frozen lung tissue samples and fetal lung fibroblasts following treatment conditions were suspended in radioimmunoprecipitation assay buffer containing 1 mM EDTA and EGTA (Boston BioProducts, Ashland, MA) supplemented with 1 mM PMSF and a complete protease inhibitor mixture (Roche Diagnostics, Indianapolis, IN), sonicated, and centrifuged at 4°C for 10 min at 14,000 rpm. .. SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific Pierce, Rockford, IL) was used for detection, and photographic emulsion was used to identify the protein bands, which were subsequently quantified by densitometry.

    Injection:

    Article Title: Artificial miRNAs Reduce Human Mutant Huntingtin Throughout the Striatum in a Transgenic Sheep Model of Huntington's Disease
    Article Snippet: Blots were washed in TBST, incubated in peroxidase labeled secondary antibodies (Jackson Immunoresearch), diluted 1:5,000 in blocking solution for 1 h at room temperature, washed in TBST, and incubated in SuperSignal West Pico Chemiluminescent Substrate (Pierce #34080). .. Statistical analysis was performed using unpaired t -tests, and results were expressed as mean value for the injected side.

    Recombinant:

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: Paragraph title: Analysis of recombinant protein expression by SDS-PAGE and Western blot ... The bands were detected by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA).

    Molecular Weight:

    Article Title: The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology
    Article Snippet: Gels were prepared using Mini-PROTEAN Tetra handcast systems (BioRad) and the Precision Plus Protein Kaleidoscope Prestained Protein Standards marker (Bio-Rad) was loaded to show the migration of molecular weight standards. .. Membranes were once again washed three times before development using SuperSignal West Pico Chemiluminescent substrate (ThermoFisher) and imaging using a LAS-3000 imager (Fujifilm).

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: The bands were detected by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA). .. A home-made luminescent strip put on top of PVDF membrane for immunoreactive protein detection was used and arranged in the same place as the SDS-PAGE molecular weight marker.

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: The immunoreactive proteins were revealed by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA). .. A home-made luminescent strip put on top of PVDF membrane for immunoreactive protein detection was used and arranged in the same place as the SDS-PAGE molecular weight marker.

    Mutagenesis:

    Article Title: An inter‐dimer allosteric switch controls NMDA receptor activity
    Article Snippet: Oocytes expressing “silent” mutant receptors were selected based on redox treatment of other oocytes of the same batch revealing high expression of “silent” receptors. .. Protein bands were visualized using secondary goat peroxidase‐linked anti‐mouse antibodies (1:10,000, Jackson ImmunoResearch catalog number #115‐035‐003), with the SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Scientific™).

    Labeling:

    Article Title: Artificial miRNAs Reduce Human Mutant Huntingtin Throughout the Striatum in a Transgenic Sheep Model of Huntington's Disease
    Article Snippet: .. Blots were washed in TBST, incubated in peroxidase labeled secondary antibodies (Jackson Immunoresearch), diluted 1:5,000 in blocking solution for 1 h at room temperature, washed in TBST, and incubated in SuperSignal West Pico Chemiluminescent Substrate (Pierce #34080). .. Images were obtained with a CCD imaging system (Alpha Innotech) and Hyperfilm ECL (GE Healthcare).

    Protein Extraction:

    Article Title: The p75 Neurotrophin Receptor Is an Essential Mediator of Impairments in Hippocampal-Dependent Associative Plasticity and Memory Induced by Sleep Deprivation
    Article Snippet: The tissues were then lysed in Tissue Protein Extraction reagent (T-PER; ThermoFisher Scientific) with protease and phosphatase inhibitor (ThermoFisher Scientific), followed by centrifugation for 5 min at 10,000 rpm at 4°C. .. Signals were generated by using the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific).

    Article Title: Long non-coding RNA PSMB8-AS1 regulates influenza virus replication
    Article Snippet: Cells were lysed in M-PER™ Mammalian Protein Extraction Reagent (Thermo Fischer, Waltham, MA, USA). .. Finally, the membranes were developed using SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Fischer, Waltham, MA, USA).

    Bradford Protein Assay:

    Article Title: The p75 Neurotrophin Receptor Is an Essential Mediator of Impairments in Hippocampal-Dependent Associative Plasticity and Memory Induced by Sleep Deprivation
    Article Snippet: Signals were generated by using the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific). .. Signals were generated by using the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific).

    Staining:

    Article Title: The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology
    Article Snippet: Samples were stained with Coomassie Brilliant Blue R250 (Sigma) or subjected to immunoblotting. .. Membranes were once again washed three times before development using SuperSignal West Pico Chemiluminescent substrate (ThermoFisher) and imaging using a LAS-3000 imager (Fujifilm).

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: SDS-PAGE were stained with Coomassie Brilliant Blue R-250 (Bio-Rad, Hercules, CA, USA), and the excess dye was removed using a solution containing 10% acetic acid, 50% methanol, and water. .. The bands were detected by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA).

    SDS Page:

    Article Title: phlda3 overexpression impairs specification of hemangioblasts and vascular development
    Article Snippet: Protein extracts from 24 hpf stage zebrafish embryos (n=80) were prepared as previously described [ ], separated by 10% SDS-PAGE, and then transferred to PVDF membranes. .. Signals were detected by incubation with a SuperSignal West Pico Chemiluminescent Substrate (Thermo) and ChemiDoc XRS (Bio-Rad).

    Article Title: Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice.
    Article Snippet: The protein extracts were separated by SDS-PAGE, transferred to Hybond-ECL nitrocellulose membrane (GE Healthcare, Chalfont St. Giles, United Kingdom), which was incubated with a blocking buffer (0.1% Tween and 5% non-fat dry milk in PBS), and then with anti-N and anti-P polyclonal antibodies previously obtained (Simabuco et al., 2007) . .. After three washes with washing solution, the membrane was treated with SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology, Rockford, USA), exposed to Hyperfilm (Amersham) and visualized.

    Article Title: An inter‐dimer allosteric switch controls NMDA receptor activity
    Article Snippet: Samples were separated on 3–8% SDS–PAGE gradient gels (4 oocytes per lane), semi‐dry transferred to nitrocellulose membrane, and immunoblotted with anti‐GluN1 antibody (1:750, mouse monoclonal MAB1586 clone R1JHL, Millipore) or anti‐GluN2B antibody (1:500, mouse monoclonal 75‐101 clone N59/36, NeuroMab). .. Protein bands were visualized using secondary goat peroxidase‐linked anti‐mouse antibodies (1:10,000, Jackson ImmunoResearch catalog number #115‐035‐003), with the SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Scientific™).

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: Paragraph title: Analysis of recombinant protein expression by SDS-PAGE and Western blot ... The bands were detected by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA).

    Article Title: Long non-coding RNA PSMB8-AS1 regulates influenza virus replication
    Article Snippet: Proteins (30 µg/lane) were separated by 12% of SDS-PAGE and transferred onto nitrocellulose membranes using Trans-Blot® Turbo™ Transfer System (Bio-Rad, Hercules, CA, USA). .. Finally, the membranes were developed using SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Fischer, Waltham, MA, USA).

    Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
    Article Snippet: Equal aliquots (50 μg) of total protein for each sample were denatured with SDS-PAGE sample buffer and separated by electrophoresis on an SDS polyacrylamide gel. .. SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific Pierce, Rockford, IL) was used for detection, and photographic emulsion was used to identify the protein bands, which were subsequently quantified by densitometry.

    Article Title: A Matricryptic Conformation of the Integrin-Binding Domain of Fibronectin Regulates Platelet-Derived Growth Factor-Induced Intracellular Calcium Release
    Article Snippet: Immunoblotting Cells were solubilized in Laemmli buffer and lysates (~40 μg protein per lane) were analyzed by SDS-PAGE and immunoblotting, as described previously [ ]. .. Blots were washed with TBS-T, incubated with goat anti-rabbit (1:5000) or goat anti-mouse (1:20,000) horseradish peroxidase-linked secondary antibodies, and developed using SuperSignal® West Pico Chemiluminescent Substrate (Thermo Fisher Scientific).

    Article Title: Artificial miRNAs Reduce Human Mutant Huntingtin Throughout the Striatum in a Transgenic Sheep Model of Huntington's Disease
    Article Snippet: Equal concentrations of protein (25 μg) were separated by SDS-PAGE on 3–8% Tris-Acetate gels (Life Technologies; #EA03785BOX) and transferred to nitrocellulose using TransBlot Turbo (Bio-Rad). .. Blots were washed in TBST, incubated in peroxidase labeled secondary antibodies (Jackson Immunoresearch), diluted 1:5,000 in blocking solution for 1 h at room temperature, washed in TBST, and incubated in SuperSignal West Pico Chemiluminescent Substrate (Pierce #34080).

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: The immunoreactive proteins were revealed by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA). .. A home-made luminescent strip put on top of PVDF membrane for immunoreactive protein detection was used and arranged in the same place as the SDS-PAGE molecular weight marker.

    Software:

    Article Title: The p75 Neurotrophin Receptor Is an Essential Mediator of Impairments in Hippocampal-Dependent Associative Plasticity and Memory Induced by Sleep Deprivation
    Article Snippet: Signals were generated by using the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific). .. The amount of protein was quantified by densitometric measurement of Western blots using ImageJ (NIH software).

    Article Title: Contemporary Circulating Enterovirus D68 Strains Infect and Undergo Retrograde Axonal Transport in Spinal Motor Neurons Independent of Sialic Acid
    Article Snippet: Following five washes in TBST, the blots were incubated in secondary antibody in 5% dry milk in TBST for 1 h. After five washes in TBST, the blots were developed using SuperSignal West Pico chemiluminescent substrate (catalog number 34580; Thermo Fisher) and imaged using a FluorochemQ MultiImage III workstation. .. Images were brightened linearly and equally using FluorochemQ image software.

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: Quantification was done by densitometry in the SDS-PAGE using the Image-Lab™ software and Gel Doc™ EZ Imager (Bio-Rad, Hercules, CA, USA). .. The bands were detected by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA).

    Article Title: Long non-coding RNA PSMB8-AS1 regulates influenza virus replication
    Article Snippet: Finally, the membranes were developed using SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Fischer, Waltham, MA, USA). .. Protein levels were normalized to β-actin and quantitation was determined using Image Quant software from GE healthcare system.

    Article Title: A Matricryptic Conformation of the Integrin-Binding Domain of Fibronectin Regulates Platelet-Derived Growth Factor-Induced Intracellular Calcium Release
    Article Snippet: Blots were washed with TBS-T, incubated with goat anti-rabbit (1:5000) or goat anti-mouse (1:20,000) horseradish peroxidase-linked secondary antibodies, and developed using SuperSignal® West Pico Chemiluminescent Substrate (Thermo Fisher Scientific). .. The average net band intensity for duplicate samples was determined by densitometry using Carestream Molecular Imaging Software (Rochester, NY, USA).

    Article Title: Artificial miRNAs Reduce Human Mutant Huntingtin Throughout the Striatum in a Transgenic Sheep Model of Huntington's Disease
    Article Snippet: Blots were washed in TBST, incubated in peroxidase labeled secondary antibodies (Jackson Immunoresearch), diluted 1:5,000 in blocking solution for 1 h at room temperature, washed in TBST, and incubated in SuperSignal West Pico Chemiluminescent Substrate (Pierce #34080). .. Densitometry was performed on the digital images using ImageJ software (NIH).

    Radio Immunoprecipitation:

    Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
    Article Snippet: Liquid nitrogen flash-frozen lung tissue samples and fetal lung fibroblasts following treatment conditions were suspended in radioimmunoprecipitation assay buffer containing 1 mM EDTA and EGTA (Boston BioProducts, Ashland, MA) supplemented with 1 mM PMSF and a complete protease inhibitor mixture (Roche Diagnostics, Indianapolis, IN), sonicated, and centrifuged at 4°C for 10 min at 14,000 rpm. .. SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific Pierce, Rockford, IL) was used for detection, and photographic emulsion was used to identify the protein bands, which were subsequently quantified by densitometry.

    Quantitation Assay:

    Article Title: Long non-coding RNA PSMB8-AS1 regulates influenza virus replication
    Article Snippet: Finally, the membranes were developed using SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Fischer, Waltham, MA, USA). .. Protein levels were normalized to β-actin and quantitation was determined using Image Quant software from GE healthcare system.

    Concentration Assay:

    Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
    Article Snippet: The concentration of protein was determined using the BCA Protein Assay Kit (Thermo Scientific Pierce, Rockford, IL). .. SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific Pierce, Rockford, IL) was used for detection, and photographic emulsion was used to identify the protein bands, which were subsequently quantified by densitometry.

    Migration:

    Article Title: The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology
    Article Snippet: Gels were prepared using Mini-PROTEAN Tetra handcast systems (BioRad) and the Precision Plus Protein Kaleidoscope Prestained Protein Standards marker (Bio-Rad) was loaded to show the migration of molecular weight standards. .. Membranes were once again washed three times before development using SuperSignal West Pico Chemiluminescent substrate (ThermoFisher) and imaging using a LAS-3000 imager (Fujifilm).

    Marker:

    Article Title: The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology
    Article Snippet: Gels were prepared using Mini-PROTEAN Tetra handcast systems (BioRad) and the Precision Plus Protein Kaleidoscope Prestained Protein Standards marker (Bio-Rad) was loaded to show the migration of molecular weight standards. .. Membranes were once again washed three times before development using SuperSignal West Pico Chemiluminescent substrate (ThermoFisher) and imaging using a LAS-3000 imager (Fujifilm).

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: The bands were detected by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA). .. A home-made luminescent strip put on top of PVDF membrane for immunoreactive protein detection was used and arranged in the same place as the SDS-PAGE molecular weight marker.

    Article Title: Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies
    Article Snippet: The immunoreactive proteins were revealed by chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Sci., Waltham, MA, USA) and visualized using the C-DIGIT blot scanner (LI-COR, Lincoln, NE, USA). .. A home-made luminescent strip put on top of PVDF membrane for immunoreactive protein detection was used and arranged in the same place as the SDS-PAGE molecular weight marker.

    Lysis:

    Article Title: An inter‐dimer allosteric switch controls NMDA receptor activity
    Article Snippet: Each mix of 16 oocytes was then homogenized on ice by back and forth pipetting with 160 μl of lysis buffer (20 mM Tris pH 8.0, 50 mM NaCl, 1% DDM, 1/20 of a complete protease inhibitor cocktail tablet, Roche Complete, Mini) until a homogenous suspension was obtained. .. Protein bands were visualized using secondary goat peroxidase‐linked anti‐mouse antibodies (1:10,000, Jackson ImmunoResearch catalog number #115‐035‐003), with the SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Scientific™).

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    Thermo Fisher super signal west pico chemiluminescense substrate
    Super Signal West Pico Chemiluminescense Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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