superscriptiii transcriptase  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 87

    Structured Review

    Thermo Fisher superscriptiii transcriptase
    Superscriptiii Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiii transcriptase/product/Thermo Fisher
    Average 87 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    superscriptiii transcriptase - by Bioz Stars, 2019-12
    87/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.)
    Article Snippet: cDNAs were synthesized with SuperScriptIII transcriptase (Invitrogen, Carlsbad, CA, USA) and an adapter primer from total RNA (1 μg as quantified by UV spectrometer) from fruits of each tree. .. cDNAs were synthesized with SuperScriptIII transcriptase (Invitrogen, Carlsbad, CA, USA) and an adapter primer from total RNA (1 μg as quantified by UV spectrometer) from fruits of each tree.

    Transfection:

    Article Title: The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation
    Article Snippet: Transfection efficiency was corrected using the 2051 kb Tfam promoter firefly luciferase construct (0.4 μg/well) and normalized to day 0 of Tfam promoter luciferase activity. .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions.

    Luciferase:

    Article Title: The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation
    Article Snippet: Transfection efficiency was corrected using the 2051 kb Tfam promoter firefly luciferase construct (0.4 μg/well) and normalized to day 0 of Tfam promoter luciferase activity. .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions.

    Synthesized:

    Article Title: Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.)
    Article Snippet: The cDNAs obtained from the screening were subcloned into a pBluescript KS vector and their sequences were determined as described above. .. cDNAs were synthesized with SuperScriptIII transcriptase (Invitrogen, Carlsbad, CA, USA) and an adapter primer from total RNA (1 μg as quantified by UV spectrometer) from fruits of each tree. .. Sequences of primer pairs used for amplification are listed in Supplementary Table S2.

    Article Title: Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit
    Article Snippet: Hybridization temperature was set within a range of 53°C to 65°C to isolate heterogenous orthologs. .. Total RNA was isolated from each frozen organ by the hot-borate method ( ). cDNA was synthesized from 1 μg of total RNA using SuperScriptIII transcriptase (Invitrogen) and an adopter primer. .. Expression analysis was performed by quantitative real-time (qRT)-PCR analysis.

    Isolation:

    Article Title: Circadian Regulation of the Na+/K+-Atpase Alpha Subunit in the Visual System Is Mediated by the Pacemaker and by Retina Photoreceptors in Drosophila Melanogaster
    Article Snippet: Retinas were cut off manually at ZT1 and total RNA was isolated using NucleoSpin RNA XS kit (Macherey-Nagel Germany) according to the manufacturer's protocol. .. 2 μg of total RNA was used for reverse transcription using a poly-T oligo and SuperScriptIII transcriptase (Invitrogen).

    Article Title: Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.)
    Article Snippet: cDNAs were synthesized with SuperScriptIII transcriptase (Invitrogen, Carlsbad, CA, USA) and an adapter primer from total RNA (1 μg as quantified by UV spectrometer) from fruits of each tree. .. Primers for SCPL were from the cDNA sequence reported previously (Ikegami et al. ).

    Article Title: Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit
    Article Snippet: Hybridization temperature was set within a range of 53°C to 65°C to isolate heterogenous orthologs. .. Total RNA was isolated from each frozen organ by the hot-borate method ( ). cDNA was synthesized from 1 μg of total RNA using SuperScriptIII transcriptase (Invitrogen) and an adopter primer. .. Expression analysis was performed by quantitative real-time (qRT)-PCR analysis.

    Article Title: Angiogenic properties of aged adipose derived mesenchymal stem cells after hypoxic conditioning
    Article Snippet: RNA was isolated according to instructions (RNA/DNA/Protein Purification Kit, Norgen, Canada). .. One μg of total RNA was used to synthesize cDNA with SuperscriptIII-transcriptase (70 U/1 μg RNA; Invitrogen) and Oligo(dT)18-Primers (Fermentas, see Table for the different primer sequences used).

    Sequencing:

    Article Title: Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.)
    Article Snippet: cDNAs were synthesized with SuperScriptIII transcriptase (Invitrogen, Carlsbad, CA, USA) and an adapter primer from total RNA (1 μg as quantified by UV spectrometer) from fruits of each tree. .. Primers for GST, Prx, and F3GT were designed based on the sequences of the full-coding cDNA clones, which were obtained as described above.

    Pyrolysis Gas Chromatography:

    Article Title: Acute exercise induces tumour suppressor protein p53 translocation to the mitochondria and promotes a p53–Tfam–mitochondrial DNA complex in skeletal muscle
    Article Snippet: The mRNA expression of PPAR-γ co-activator 1-α ( PGC-1α ), mitochondrial transcription factor A ( Tfam ), nuclear respiratory factor-1 ( NRF-1 ), cytochrome c oxidase subunits I and IV ( COX-I, COX-IV ), citrate synthase ( CS ), glyceraldehye phosphate dehydrogenase ( GAPDH ) and p53 was quantified using a 7500 Real-Time PCR System (Applied Biosystems Inc., Foster City, CA, USA) and SYBR Green chemistry (PerfeCT a SYBR Green Supermix, ROX, Quanta BioSciences, Gaithersburg, MD, USA). .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to the manufacturer's directions.

    Construct:

    Article Title: The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation
    Article Snippet: Transfection efficiency was corrected using the 2051 kb Tfam promoter firefly luciferase construct (0.4 μg/well) and normalized to day 0 of Tfam promoter luciferase activity. .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions.

    Quantitative RT-PCR:

    Article Title: Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.)
    Article Snippet: Paragraph title: qRT-PCR analysis ... cDNAs were synthesized with SuperScriptIII transcriptase (Invitrogen, Carlsbad, CA, USA) and an adapter primer from total RNA (1 μg as quantified by UV spectrometer) from fruits of each tree.

    SYBR Green Assay:

    Article Title: The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation
    Article Snippet: The mRNA expression of mitochondrial transcription factor A (Tfam) over days of differentiation was quantified using 7300 StepOnePlus™ Real-time PCR System (Applied Biosystems Inc.) and SYBR® Green chemistry (PerfeCT a SYBR® Green Supermix, ROX, Quanta BioSciences). .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions.

    Article Title: Acute exercise induces tumour suppressor protein p53 translocation to the mitochondria and promotes a p53–Tfam–mitochondrial DNA complex in skeletal muscle
    Article Snippet: The mRNA expression of PPAR-γ co-activator 1-α ( PGC-1α ), mitochondrial transcription factor A ( Tfam ), nuclear respiratory factor-1 ( NRF-1 ), cytochrome c oxidase subunits I and IV ( COX-I, COX-IV ), citrate synthase ( CS ), glyceraldehye phosphate dehydrogenase ( GAPDH ) and p53 was quantified using a 7500 Real-Time PCR System (Applied Biosystems Inc., Foster City, CA, USA) and SYBR Green chemistry (PerfeCT a SYBR Green Supermix, ROX, Quanta BioSciences, Gaithersburg, MD, USA). .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to the manufacturer's directions.

    Article Title: Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit
    Article Snippet: Total RNA was isolated from each frozen organ by the hot-borate method ( ). cDNA was synthesized from 1 μg of total RNA using SuperScriptIII transcriptase (Invitrogen) and an adopter primer. .. An aliquot of 1:5 diluted pool cDNA was used as a template for PCR.

    Concentration Assay:

    Article Title: Angiogenic properties of aged adipose derived mesenchymal stem cells after hypoxic conditioning
    Article Snippet: RNA concentration was measured using the NanoDrop (Peqlab). .. One μg of total RNA was used to synthesize cDNA with SuperscriptIII-transcriptase (70 U/1 μg RNA; Invitrogen) and Oligo(dT)18-Primers (Fermentas, see Table for the different primer sequences used).

    Generated:

    Article Title: The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation
    Article Snippet: First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions. .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions.

    Article Title: Acute exercise induces tumour suppressor protein p53 translocation to the mitochondria and promotes a p53–Tfam–mitochondrial DNA complex in skeletal muscle
    Article Snippet: First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to the manufacturer's directions. .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to the manufacturer's directions.

    Amplification:

    Article Title: The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation
    Article Snippet: First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions. .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions.

    Article Title: Circadian Regulation of the Na+/K+-Atpase Alpha Subunit in the Visual System Is Mediated by the Pacemaker and by Retina Photoreceptors in Drosophila Melanogaster
    Article Snippet: 2 μg of total RNA was used for reverse transcription using a poly-T oligo and SuperScriptIII transcriptase (Invitrogen). .. 2 μg of total RNA was used for reverse transcription using a poly-T oligo and SuperScriptIII transcriptase (Invitrogen).

    Article Title: Acute exercise induces tumour suppressor protein p53 translocation to the mitochondria and promotes a p53–Tfam–mitochondrial DNA complex in skeletal muscle
    Article Snippet: First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to the manufacturer's directions. .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to the manufacturer's directions.

    Article Title: Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit
    Article Snippet: Total RNA was isolated from each frozen organ by the hot-borate method ( ). cDNA was synthesized from 1 μg of total RNA using SuperScriptIII transcriptase (Invitrogen) and an adopter primer. .. Expression analysis was performed by quantitative real-time (qRT)-PCR analysis.

    Activity Assay:

    Article Title: The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation
    Article Snippet: Transfection efficiency was corrected using the 2051 kb Tfam promoter firefly luciferase construct (0.4 μg/well) and normalized to day 0 of Tfam promoter luciferase activity. .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions.

    Labeling:

    Article Title: Circadian Regulation of the Na+/K+-Atpase Alpha Subunit in the Visual System Is Mediated by the Pacemaker and by Retina Photoreceptors in Drosophila Melanogaster
    Article Snippet: 2 μg of total RNA was used for reverse transcription using a poly-T oligo and SuperScriptIII transcriptase (Invitrogen). .. The resulting cDNA was diluted 1∶8 and then used for quantitative PCR.

    Expressing:

    Article Title: The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation
    Article Snippet: The mRNA expression of mitochondrial transcription factor A (Tfam) over days of differentiation was quantified using 7300 StepOnePlus™ Real-time PCR System (Applied Biosystems Inc.) and SYBR® Green chemistry (PerfeCT a SYBR® Green Supermix, ROX, Quanta BioSciences). .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions.

    Article Title: Circadian Regulation of the Na+/K+-Atpase Alpha Subunit in the Visual System Is Mediated by the Pacemaker and by Retina Photoreceptors in Drosophila Melanogaster
    Article Snippet: The expression of UAS-cry-RNAi in photoreceptor cells using the gmr-GAL4 driver resulted in a reduction of 36% in cry mRNA compared to the gmr-GAL4 driver control (set to 1). .. 2 μg of total RNA was used for reverse transcription using a poly-T oligo and SuperScriptIII transcriptase (Invitrogen).

    Article Title: Acute exercise induces tumour suppressor protein p53 translocation to the mitochondria and promotes a p53–Tfam–mitochondrial DNA complex in skeletal muscle
    Article Snippet: Paragraph title: mRNA expression analyses ... First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to the manufacturer's directions.

    Article Title: Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit
    Article Snippet: Paragraph title: Expression Analysis ... Total RNA was isolated from each frozen organ by the hot-borate method ( ). cDNA was synthesized from 1 μg of total RNA using SuperScriptIII transcriptase (Invitrogen) and an adopter primer.

    Polymerase Chain Reaction:

    Article Title: Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit
    Article Snippet: Total RNA was isolated from each frozen organ by the hot-borate method ( ). cDNA was synthesized from 1 μg of total RNA using SuperScriptIII transcriptase (Invitrogen) and an adopter primer. .. Total RNA was isolated from each frozen organ by the hot-borate method ( ). cDNA was synthesized from 1 μg of total RNA using SuperScriptIII transcriptase (Invitrogen) and an adopter primer.

    Real-time Polymerase Chain Reaction:

    Article Title: The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation
    Article Snippet: The mRNA expression of mitochondrial transcription factor A (Tfam) over days of differentiation was quantified using 7300 StepOnePlus™ Real-time PCR System (Applied Biosystems Inc.) and SYBR® Green chemistry (PerfeCT a SYBR® Green Supermix, ROX, Quanta BioSciences). .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to manufacturer's directions.

    Article Title: Circadian Regulation of the Na+/K+-Atpase Alpha Subunit in the Visual System Is Mediated by the Pacemaker and by Retina Photoreceptors in Drosophila Melanogaster
    Article Snippet: Quantification was carried out by reverse transcription real time PCR as described below. .. 2 μg of total RNA was used for reverse transcription using a poly-T oligo and SuperScriptIII transcriptase (Invitrogen).

    Article Title: Acute exercise induces tumour suppressor protein p53 translocation to the mitochondria and promotes a p53–Tfam–mitochondrial DNA complex in skeletal muscle
    Article Snippet: The mRNA expression of PPAR-γ co-activator 1-α ( PGC-1α ), mitochondrial transcription factor A ( Tfam ), nuclear respiratory factor-1 ( NRF-1 ), cytochrome c oxidase subunits I and IV ( COX-I, COX-IV ), citrate synthase ( CS ), glyceraldehye phosphate dehydrogenase ( GAPDH ) and p53 was quantified using a 7500 Real-Time PCR System (Applied Biosystems Inc., Foster City, CA, USA) and SYBR Green chemistry (PerfeCT a SYBR Green Supermix, ROX, Quanta BioSciences, Gaithersburg, MD, USA). .. First-strand cDNA synthesis from 2 μg of total RNA was performed with primers using SuperscriptIII transcriptase (Invitrogen) according to the manufacturer's directions.

    Article Title: Angiogenic properties of aged adipose derived mesenchymal stem cells after hypoxic conditioning
    Article Snippet: Paragraph title: Real-time PCR ... One μg of total RNA was used to synthesize cDNA with SuperscriptIII-transcriptase (70 U/1 μg RNA; Invitrogen) and Oligo(dT)18-Primers (Fermentas, see Table for the different primer sequences used).

    Purification:

    Article Title: Angiogenic properties of aged adipose derived mesenchymal stem cells after hypoxic conditioning
    Article Snippet: RNA was isolated according to instructions (RNA/DNA/Protein Purification Kit, Norgen, Canada). .. One μg of total RNA was used to synthesize cDNA with SuperscriptIII-transcriptase (70 U/1 μg RNA; Invitrogen) and Oligo(dT)18-Primers (Fermentas, see Table for the different primer sequences used).

    Derivative Assay:

    Article Title: Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.)
    Article Snippet: cDNAs were synthesized with SuperScriptIII transcriptase (Invitrogen, Carlsbad, CA, USA) and an adapter primer from total RNA (1 μg as quantified by UV spectrometer) from fruits of each tree. .. Primers for SCPL were from the cDNA sequence reported previously (Ikegami et al. ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher superscriptiii reverse transcriptase
    Superscriptiii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiii reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 126 article reviews
    Price from $9.99 to $1999.99
    superscriptiii reverse transcriptase - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher superscriptii reverse transcriptase
    Superscriptii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptii reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    superscriptii reverse transcriptase - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

    Image Search Results