superscriptiii rnase h reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscriptiii rnase h reverse transcriptase
    Superscriptiii Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiii rnase h reverse transcriptase/product/Thermo Fisher
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscriptiii rnase h reverse transcriptase - by Bioz Stars, 2020-02
    83/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1 [W]Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1 [W] [OA]
    Article Snippet: RNA was electrophoresed on 1% agarose gel to confirm integrity. cDNA was synthesized from 2 μ g of RNA with 100 units of SuperScriptIII RNase H− reverse transcriptase (Invitrogen) and 100 ng of random hexamers in a 20- μ L reaction. .. Quantitative real-time reverse transcription (RT)-PCR was performed in a 25- μ L reaction containing 12.5 μ L of 2 × SYBR Green Supermix (Bio-Rad), 100 pmol of the forward and reverse primer, and 10 ng of cDNA, except 0.1 ng of cDNA was used for Arabidopsis 18S rRNA.

    Amplification:

    Article Title: Activation of peroxisome proliferator-activated receptor gamma suppresses mast cell maturation involved in allergic diseases
    Article Snippet: Total RNA was extracted from the BMMCs, and first-strand cDNA was generated from 1 µg of RNA by using oligo (dT)12–18 primer and SuperScriptIII RNase H Reverse Transcriptase (Invitrogen) according to the manufacturer’s protocol. .. The reaction mixture was amplified with the oligonucleotides specific for HDC, MMCP-1, MMCP-2, MMCP-4 and β-actin ( – ).

    Agarose Gel Electrophoresis:

    Article Title: Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1 [W]Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1 [W] [OA]
    Article Snippet: .. RNA was electrophoresed on 1% agarose gel to confirm integrity. cDNA was synthesized from 2 μ g of RNA with 100 units of SuperScriptIII RNase H− reverse transcriptase (Invitrogen) and 100 ng of random hexamers in a 20- μ L reaction. .. Quantitative real-time reverse transcription (RT)-PCR was performed in a 25- μ L reaction containing 12.5 μ L of 2 × SYBR Green Supermix (Bio-Rad), 100 pmol of the forward and reverse primer, and 10 ng of cDNA, except 0.1 ng of cDNA was used for Arabidopsis 18S rRNA.

    Synthesized:

    Article Title: Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1 [W]Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1 [W] [OA]
    Article Snippet: .. RNA was electrophoresed on 1% agarose gel to confirm integrity. cDNA was synthesized from 2 μ g of RNA with 100 units of SuperScriptIII RNase H− reverse transcriptase (Invitrogen) and 100 ng of random hexamers in a 20- μ L reaction. .. Quantitative real-time reverse transcription (RT)-PCR was performed in a 25- μ L reaction containing 12.5 μ L of 2 × SYBR Green Supermix (Bio-Rad), 100 pmol of the forward and reverse primer, and 10 ng of cDNA, except 0.1 ng of cDNA was used for Arabidopsis 18S rRNA.

    Isolation:

    Article Title: Activation of peroxisome proliferator-activated receptor gamma suppresses mast cell maturation involved in allergic diseases
    Article Snippet: Paragraph title: RNA isolation and RT-PCR analysis ... Total RNA was extracted from the BMMCs, and first-strand cDNA was generated from 1 µg of RNA by using oligo (dT)12–18 primer and SuperScriptIII RNase H Reverse Transcriptase (Invitrogen) according to the manufacturer’s protocol.

    Real-time Polymerase Chain Reaction:

    Article Title: Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1 [W]Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1 [W] [OA]
    Article Snippet: RNA was electrophoresed on 1% agarose gel to confirm integrity. cDNA was synthesized from 2 μ g of RNA with 100 units of SuperScriptIII RNase H− reverse transcriptase (Invitrogen) and 100 ng of random hexamers in a 20- μ L reaction. .. A Bio-Rad MyiQ single-color real-time PCR detection system (Bio-Rad) was used with the following thermal profile for all genes: 3 min at 95°C, followed by 40 cycles of 30 s at 95° s, 30 s at 62°C, and 60 s at 72°C.

    Generated:

    Article Title: Activation of peroxisome proliferator-activated receptor gamma suppresses mast cell maturation involved in allergic diseases
    Article Snippet: .. Total RNA was extracted from the BMMCs, and first-strand cDNA was generated from 1 µg of RNA by using oligo (dT)12–18 primer and SuperScriptIII RNase H Reverse Transcriptase (Invitrogen) according to the manufacturer’s protocol. .. The reaction mixture was amplified with the oligonucleotides specific for HDC, MMCP-1, MMCP-2, MMCP-4 and β-actin ( – ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1 [W]Cytokinin Import Rate as a Signal for Photosynthetic Acclimation to Canopy Light Gradients 1 [W] [OA]
    Article Snippet: RNA was electrophoresed on 1% agarose gel to confirm integrity. cDNA was synthesized from 2 μ g of RNA with 100 units of SuperScriptIII RNase H− reverse transcriptase (Invitrogen) and 100 ng of random hexamers in a 20- μ L reaction. .. Quantitative real-time reverse transcription (RT)-PCR was performed in a 25- μ L reaction containing 12.5 μ L of 2 × SYBR Green Supermix (Bio-Rad), 100 pmol of the forward and reverse primer, and 10 ng of cDNA, except 0.1 ng of cDNA was used for Arabidopsis 18S rRNA.

    Article Title: Activation of peroxisome proliferator-activated receptor gamma suppresses mast cell maturation involved in allergic diseases
    Article Snippet: Paragraph title: RNA isolation and RT-PCR analysis ... Total RNA was extracted from the BMMCs, and first-strand cDNA was generated from 1 µg of RNA by using oligo (dT)12–18 primer and SuperScriptIII RNase H Reverse Transcriptase (Invitrogen) according to the manufacturer’s protocol.