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TaKaRa superscriptiii reverse transcriptase
Superscriptiii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscriptiii reverse transcriptase/product/TaKaRa
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
superscriptiii reverse transcriptase - by Bioz Stars, 2020-04
89/100 stars

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Related Articles

Real-time Polymerase Chain Reaction:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: The first cDNA strand was synthesized by SuperScriptIII Reverse Transcriptase (Takara), according to the manufacturer’s instructions. .. Quantitative real-time PCR (qRT-PCR) was performed using SYBR Premix Ex TaqTMII (Takara).

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: Paragraph title: RNA extraction, primer design and quantitative real-time PCR ... For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions.

RNA Extraction:

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: Paragraph title: RNA extraction, primer design and quantitative real-time PCR ... For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions.

Amplification:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: A 1 μL aliquot of the resulting RNA (containing about 600 ng) provided the template for the synthesis of the first cDNA strand by SuperScriptIII Reverse Transcriptase (Takara) with random hexamer primers. .. To obtain the full length cDNA prior to a RACE PCR, the sequences were first validated by amplification with a pair of gene-specific primers (SP-F/SP-R: sequences given in Supplementary Table ; Supplementary Figure ).

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: The gene’s open reading frame (ORF) was identified using www.ncbi.nlm.nih.gov/gorf/gorf.html and amplified using primers Full-F/Full-R (sequences given in Supplementary Table ). .. The first cDNA strand was synthesized by SuperScriptIII Reverse Transcriptase (Takara), according to the manufacturer’s instructions.

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions. .. To enhance specificity of the primer, most of them were cross-blasted the ORF and 3’UTR of VvbZIP genes, all of the primers were tested with PCR amplification, electrophoresis gels, melting curve analysis and the products of PCR amplification were sequenced at least three times, the maximum deviation of which were less than 3 base pairs.

Agarose Gel Electrophoresis:

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: The qualities and quantities of RNA were determined by agarose gel electrophoresis and Nanodrop ND-1000 Spectrophotometer (Thermo Fisher Scientific Inc.; USA). .. For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions.

Synthesized:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: A 1 μL aliquot of the resulting RNA (containing about 600 ng) provided the template for the synthesis of the first cDNA strand by SuperScriptIII Reverse Transcriptase (Takara) with random hexamer primers. .. For the 3′ RACE, the first cDNA strand was synthesized using an oligo (dT) primer incorporating the sequence of the adaptor primer, followed by a nested PCR using the gene-specific primer pair GSP3-1/3-2/3-3 and the adaptor primer (sequences given in Supplementary Table ).

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: .. The first cDNA strand was synthesized by SuperScriptIII Reverse Transcriptase (Takara), according to the manufacturer’s instructions. .. Quantitative real-time PCR (qRT-PCR) was performed using SYBR Premix Ex TaqTMII (Takara).

Isolation:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: Paragraph title: The Isolation of CnMET1 and CnDDM1 and Measurement of Transcript Abundance ... A 1 μL aliquot of the resulting RNA (containing about 600 ng) provided the template for the synthesis of the first cDNA strand by SuperScriptIII Reverse Transcriptase (Takara) with random hexamer primers.

Article Title: Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress
Article Snippet: .. RNA isolation and expression analysis of pepper CaHsp20 genes Total RNA was extracted using Total RNA kit (BioTeke, Beijing, China) and reverse-transcribed using SuperScriptIII Reverse Transcriptase (Takara, Dalian, China), the operational procedure followed the manufacturer's procedure, and the cDNA was diluted to 50 ng/μL with ddH2 O for further examination. .. For expression analysis of pepper CaHsp20s in different tissues, semi-quantitative reverse-transcription (RT) PCR reactions were employed.

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: RNA extraction, primer design and quantitative real-time PCR Total RNA was extracted using Huayueyang Quick RNA isolation Kit (Cat.No. .. For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions.

Polymerase Chain Reaction:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: A 1 μL aliquot of the resulting RNA (containing about 600 ng) provided the template for the synthesis of the first cDNA strand by SuperScriptIII Reverse Transcriptase (Takara) with random hexamer primers. .. The subsequent PCR used two degenerate primer pairs (DP1/DP2 and DP3/DP4: sequences given in Supplementary Table ) which targeted MET1 , designed from an alignment of the MET polypeptides of Medicago truncatula (XP_003619753.1), Hieracium pilosella (ACX83570.1), Nicotiana tabacum (BAF36443.1), Prunus persica (AAM96952.1), Elaeis guineensis (ABW96888.1), and Arabidopsis thaliana (NP_199727.1).

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions. .. To enhance specificity of the primer, most of them were cross-blasted the ORF and 3’UTR of VvbZIP genes, all of the primers were tested with PCR amplification, electrophoresis gels, melting curve analysis and the products of PCR amplification were sequenced at least three times, the maximum deviation of which were less than 3 base pairs.

Quantitative RT-PCR:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: The first cDNA strand was synthesized by SuperScriptIII Reverse Transcriptase (Takara), according to the manufacturer’s instructions. .. Quantitative real-time PCR (qRT-PCR) was performed using SYBR Premix Ex TaqTMII (Takara).

Electrophoresis:

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions. .. To enhance specificity of the primer, most of them were cross-blasted the ORF and 3’UTR of VvbZIP genes, all of the primers were tested with PCR amplification, electrophoresis gels, melting curve analysis and the products of PCR amplification were sequenced at least three times, the maximum deviation of which were less than 3 base pairs.

Sequencing:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: A 1 μL aliquot of the resulting RNA (containing about 600 ng) provided the template for the synthesis of the first cDNA strand by SuperScriptIII Reverse Transcriptase (Takara) with random hexamer primers. .. For the 3′ RACE, the first cDNA strand was synthesized using an oligo (dT) primer incorporating the sequence of the adaptor primer, followed by a nested PCR using the gene-specific primer pair GSP3-1/3-2/3-3 and the adaptor primer (sequences given in Supplementary Table ).

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: A multiple sequence alignment of the predicted gene product with homologs present in M. truncatula, H. pilosella, N. tabacum, P. persica, E. guineensis, A. thaliana, Daucus carota (AAC39355.1), H. piloselloides (ACX83569.1), N. sylvestris (CAQ18900.1), Oryza sativa (BAG15930.1), Pisum sativum (AAC49931.1), Populus trichocarpa (XP_002305346.1), Ricinus communis (XP_002518029.1), Solanum lycopersicum (NP_001234748.1), and Zea mays (NP_001105186.1) was carried out using DNAMAN software v5.2.2 (Lynnon Biosoft, Canada), and a subsequent phylogenetic analysis was carried out using MEGA 5.0 software2 . .. The first cDNA strand was synthesized by SuperScriptIII Reverse Transcriptase (Takara), according to the manufacturer’s instructions.

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions. .. Gene-specific primers for the VvbZIP genes were designed according to the predicted mRNA sequence using Beacon Designer 7.0 software (Premier Biosoft International, USA).

Random Hexamer Labeling:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: .. A 1 μL aliquot of the resulting RNA (containing about 600 ng) provided the template for the synthesis of the first cDNA strand by SuperScriptIII Reverse Transcriptase (Takara) with random hexamer primers. .. The subsequent PCR used two degenerate primer pairs (DP1/DP2 and DP3/DP4: sequences given in Supplementary Table ) which targeted MET1 , designed from an alignment of the MET polypeptides of Medicago truncatula (XP_003619753.1), Hieracium pilosella (ACX83570.1), Nicotiana tabacum (BAF36443.1), Prunus persica (AAM96952.1), Elaeis guineensis (ABW96888.1), and Arabidopsis thaliana (NP_199727.1).

Selection:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: The first cDNA strand was synthesized by SuperScriptIII Reverse Transcriptase (Takara), according to the manufacturer’s instructions. .. The reactions were first denatured (95°C/2 min), then subjected to 40 cycles of 95°C/30 s, 55°C/30 s, 72°C/30 s. A previous study suggests that when making cross-species comparison of transcript abundance involving different ploidy levels, care needs to be taken in the selection of reference gene(s) , especially in Chrysanthemum sp. ( ).

Spectrophotometry:

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: The qualities and quantities of RNA were determined by agarose gel electrophoresis and Nanodrop ND-1000 Spectrophotometer (Thermo Fisher Scientific Inc.; USA). .. For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions.

Expressing:

Article Title: Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress
Article Snippet: .. RNA isolation and expression analysis of pepper CaHsp20 genes Total RNA was extracted using Total RNA kit (BioTeke, Beijing, China) and reverse-transcribed using SuperScriptIII Reverse Transcriptase (Takara, Dalian, China), the operational procedure followed the manufacturer's procedure, and the cDNA was diluted to 50 ng/μL with ddH2 O for further examination. .. For expression analysis of pepper CaHsp20s in different tissues, semi-quantitative reverse-transcription (RT) PCR reactions were employed.

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions. .. The sequences of primers and their products were listed in Additional file , and noted that primers for VvbZIP28 , 49 , 52 and 53 were not very specific, so it was just used in one or two treatment for determining the gene expression.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress
Article Snippet: RNA isolation and expression analysis of pepper CaHsp20 genes Total RNA was extracted using Total RNA kit (BioTeke, Beijing, China) and reverse-transcribed using SuperScriptIII Reverse Transcriptase (Takara, Dalian, China), the operational procedure followed the manufacturer's procedure, and the cDNA was diluted to 50 ng/μL with ddH2 O for further examination. .. For expression analysis of pepper CaHsp20s in different tissues, semi-quantitative reverse-transcription (RT) PCR reactions were employed.

Chloramphenicol Acetyltransferase Assay:

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: To make sure that there have no DNA contamination, the traditional digestion protocol was used to eliminate DNA in the sample (rDNase®, Cat.No. .. For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions.

Binding Assay:

Article Title: Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress
Article Snippet: RNA isolation and expression analysis of pepper CaHsp20 genes Total RNA was extracted using Total RNA kit (BioTeke, Beijing, China) and reverse-transcribed using SuperScriptIII Reverse Transcriptase (Takara, Dalian, China), the operational procedure followed the manufacturer's procedure, and the cDNA was diluted to 50 ng/μL with ddH2 O for further examination. .. Ubiquitin binding protein gene CaUBI-3 was used as the reference genes (Wan et al., ).

Nested PCR:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: A 1 μL aliquot of the resulting RNA (containing about 600 ng) provided the template for the synthesis of the first cDNA strand by SuperScriptIII Reverse Transcriptase (Takara) with random hexamer primers. .. For the 3′ RACE, the first cDNA strand was synthesized using an oligo (dT) primer incorporating the sequence of the adaptor primer, followed by a nested PCR using the gene-specific primer pair GSP3-1/3-2/3-3 and the adaptor primer (sequences given in Supplementary Table ).

Software:

Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy
Article Snippet: A multiple sequence alignment of the predicted gene product with homologs present in M. truncatula, H. pilosella, N. tabacum, P. persica, E. guineensis, A. thaliana, Daucus carota (AAC39355.1), H. piloselloides (ACX83569.1), N. sylvestris (CAQ18900.1), Oryza sativa (BAG15930.1), Pisum sativum (AAC49931.1), Populus trichocarpa (XP_002305346.1), Ricinus communis (XP_002518029.1), Solanum lycopersicum (NP_001234748.1), and Zea mays (NP_001105186.1) was carried out using DNAMAN software v5.2.2 (Lynnon Biosoft, Canada), and a subsequent phylogenetic analysis was carried out using MEGA 5.0 software2 . .. The first cDNA strand was synthesized by SuperScriptIII Reverse Transcriptase (Takara), according to the manufacturer’s instructions.

Article Title: Genome-wide analysis and expression profile of the bZIP transcription factor gene family in grapevine (Vitis vinifera)
Article Snippet: For cDNA synthesis, 500 ng high quality total RNA was reverse transcribed with oligdT and random primers using SuperScriptIII Reverse Transcriptase (TaKaRa, Dalian, China) according to the manufacturer’s instructions. .. Gene-specific primers for the VvbZIP genes were designed according to the predicted mRNA sequence using Beacon Designer 7.0 software (Premier Biosoft International, USA).

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  • 89
    TaKaRa superscriptiii reverse transcriptase
    Superscriptiii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiii reverse transcriptase/product/TaKaRa
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscriptiii reverse transcriptase - by Bioz Stars, 2020-04
    89/100 stars
      Buy from Supplier

    91
    TaKaRa superscriptii reverse transcriptase
    Superscriptii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptii reverse transcriptase/product/TaKaRa
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscriptii reverse transcriptase - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

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