superscriptiii reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscriptiii reverse transcriptase kit
    Superscriptiii Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiii reverse transcriptase kit/product/Thermo Fisher
    Average 94 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    superscriptiii reverse transcriptase kit - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Dysregulation of the Mitochondrial Proteome Occurs in Mice Lacking Adiponectin Receptor 1
    Article Snippet: Paragraph title: Quantitative Real-Time PCR ... Total RNA was isolated from each tissue using TRIzol reagent (Invitrogen, Carlsbad,CA), purified with the RNEasy Kit (Qiagen, Hilden, Germany), and reverse transcribed using the SuperScriptIII Reverse Transcriptase Kit (Invitrogen, Carlsbad, CA) ( ).

    Article Title: H3.3 Barcoding of Nucleus Accumbens Transcriptional Activity Identifies Novel Molecular Cascades Associated with Cocaine Self-administration in Mice
    Article Snippet: Paragraph title: RNA extraction and qPCR. ... Generation of cDNA was performed using the SuperScriptIII Reverse Transcriptase kit (Thermo Fisher Scientific) with 80 ng of RNA as template.

    RNA Extraction:

    Article Title: H3.3 Barcoding of Nucleus Accumbens Transcriptional Activity Identifies Novel Molecular Cascades Associated with Cocaine Self-administration in Mice
    Article Snippet: Paragraph title: RNA extraction and qPCR. ... Generation of cDNA was performed using the SuperScriptIII Reverse Transcriptase kit (Thermo Fisher Scientific) with 80 ng of RNA as template.

    Amplification:

    Article Title: Dysregulation of the Mitochondrial Proteome Occurs in Mice Lacking Adiponectin Receptor 1
    Article Snippet: Total RNA was isolated from each tissue using TRIzol reagent (Invitrogen, Carlsbad,CA), purified with the RNEasy Kit (Qiagen, Hilden, Germany), and reverse transcribed using the SuperScriptIII Reverse Transcriptase Kit (Invitrogen, Carlsbad, CA) ( ). .. SYBR-green (Invitrogen, Carlsbad, CA) was used as a probe, and amplification was monitored using the CF X96 Real-Time PCR system (Bio Rad, Munich, Germany).

    Isolation:

    Article Title: Dysregulation of the Mitochondrial Proteome Occurs in Mice Lacking Adiponectin Receptor 1
    Article Snippet: .. Total RNA was isolated from each tissue using TRIzol reagent (Invitrogen, Carlsbad,CA), purified with the RNEasy Kit (Qiagen, Hilden, Germany), and reverse transcribed using the SuperScriptIII Reverse Transcriptase Kit (Invitrogen, Carlsbad, CA) ( ). .. SYBR-green (Invitrogen, Carlsbad, CA) was used as a probe, and amplification was monitored using the CF X96 Real-Time PCR system (Bio Rad, Munich, Germany).

    Spectrophotometry:

    Article Title: H3.3 Barcoding of Nucleus Accumbens Transcriptional Activity Identifies Novel Molecular Cascades Associated with Cocaine Self-administration in Mice
    Article Snippet: RNA concentration and purity were quantified by NanoDrop spectrophotometry (Thermo Fisher Scientific). .. Generation of cDNA was performed using the SuperScriptIII Reverse Transcriptase kit (Thermo Fisher Scientific) with 80 ng of RNA as template.

    Mouse Assay:

    Article Title: Dysregulation of the Mitochondrial Proteome Occurs in Mice Lacking Adiponectin Receptor 1
    Article Snippet: Quantitative Real-Time PCR Mice were euthanized using thiopental (200 mg/kg), and hearts, kidneys and livers were removed immediately and snap frozen in liquid nitrogen. .. Total RNA was isolated from each tissue using TRIzol reagent (Invitrogen, Carlsbad,CA), purified with the RNEasy Kit (Qiagen, Hilden, Germany), and reverse transcribed using the SuperScriptIII Reverse Transcriptase Kit (Invitrogen, Carlsbad, CA) ( ).

    SYBR Green Assay:

    Article Title: Dysregulation of the Mitochondrial Proteome Occurs in Mice Lacking Adiponectin Receptor 1
    Article Snippet: Total RNA was isolated from each tissue using TRIzol reagent (Invitrogen, Carlsbad,CA), purified with the RNEasy Kit (Qiagen, Hilden, Germany), and reverse transcribed using the SuperScriptIII Reverse Transcriptase Kit (Invitrogen, Carlsbad, CA) ( ). .. SYBR-green (Invitrogen, Carlsbad, CA) was used as a probe, and amplification was monitored using the CF X96 Real-Time PCR system (Bio Rad, Munich, Germany).

    Concentration Assay:

    Article Title: H3.3 Barcoding of Nucleus Accumbens Transcriptional Activity Identifies Novel Molecular Cascades Associated with Cocaine Self-administration in Mice
    Article Snippet: RNA concentration and purity were quantified by NanoDrop spectrophotometry (Thermo Fisher Scientific). .. Generation of cDNA was performed using the SuperScriptIII Reverse Transcriptase kit (Thermo Fisher Scientific) with 80 ng of RNA as template.

    Incubation:

    Article Title: H3.3 Barcoding of Nucleus Accumbens Transcriptional Activity Identifies Novel Molecular Cascades Associated with Cocaine Self-administration in Mice
    Article Snippet: Generation of cDNA was performed using the SuperScriptIII Reverse Transcriptase kit (Thermo Fisher Scientific) with 80 ng of RNA as template. .. Reactions were performed in the Step One Plus with an initial incubation at 95°C for 10 min, and 40 subsequent cycles of 95°C for 15 s, 60°C for 30 s. The TaqMan probes used in these experiments were as follows: Npas4, Mm01227866_g1; Nptx2, Mm00479438_m1; Pmepa1, Mm00452230_g1; Vgf, Mm01204485_s1; Fosb, Mm00500401_m1; Gapdh, Mm99999915_g1.

    Expressing:

    Article Title: Dysregulation of the Mitochondrial Proteome Occurs in Mice Lacking Adiponectin Receptor 1
    Article Snippet: Total RNA was isolated from each tissue using TRIzol reagent (Invitrogen, Carlsbad,CA), purified with the RNEasy Kit (Qiagen, Hilden, Germany), and reverse transcribed using the SuperScriptIII Reverse Transcriptase Kit (Invitrogen, Carlsbad, CA) ( ). .. Data were normalized to the levels of the invariant transcript α-tubulin and are presented as arbitrary units normalized to wildtype expression levels.

    Article Title: H3.3 Barcoding of Nucleus Accumbens Transcriptional Activity Identifies Novel Molecular Cascades Associated with Cocaine Self-administration in Mice
    Article Snippet: Generation of cDNA was performed using the SuperScriptIII Reverse Transcriptase kit (Thermo Fisher Scientific) with 80 ng of RNA as template. .. ΔCt values were corrected using housekeeping gene expression levels for each sample, and fold change was calculated as 2exp(−ΔΔCt ).

    Polymerase Chain Reaction:

    Article Title: H3.3 Barcoding of Nucleus Accumbens Transcriptional Activity Identifies Novel Molecular Cascades Associated with Cocaine Self-administration in Mice
    Article Snippet: Generation of cDNA was performed using the SuperScriptIII Reverse Transcriptase kit (Thermo Fisher Scientific) with 80 ng of RNA as template. .. Reactions were prepared in 96-well optical reaction plates (ABI) with optical adhesive covers (ABI) using TaqMan Fast Universal PCR Master Mix (Invitrogen).

    Purification:

    Article Title: Dysregulation of the Mitochondrial Proteome Occurs in Mice Lacking Adiponectin Receptor 1
    Article Snippet: .. Total RNA was isolated from each tissue using TRIzol reagent (Invitrogen, Carlsbad,CA), purified with the RNEasy Kit (Qiagen, Hilden, Germany), and reverse transcribed using the SuperScriptIII Reverse Transcriptase Kit (Invitrogen, Carlsbad, CA) ( ). .. SYBR-green (Invitrogen, Carlsbad, CA) was used as a probe, and amplification was monitored using the CF X96 Real-Time PCR system (Bio Rad, Munich, Germany).

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    Thermo Fisher superscript iv first strand synthesis system
    Superscript Iv First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iv first strand synthesis system/product/Thermo Fisher
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    Thermo Fisher superscriptii reverse transcriptase kit
    Superscriptii Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptii reverse transcriptase kit/product/Thermo Fisher
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    superscriptii reverse transcriptase kit - by Bioz Stars, 2020-04
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      Buy from Supplier

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