superscriptiii reverse transciptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscriptiii reverse transciptase
    Superscriptiii Reverse Transciptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiii reverse transciptase/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscriptiii reverse transciptase - by Bioz Stars, 2020-04
    86/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Tuning Piezo ion channels to detect molecular-scale movements relevant for fine touch
    Article Snippet: Paragraph title: Real-time qPCR ... Two micrograms of RNA per reaction was reverse transcribed with SuperscriptIII Reverse Transciptase (Invitrogen) using random hexamers.

    Article Title: Viral Latency in Blood and Saliva of Simian Foamy Virus-Infected Humans
    Article Snippet: RT-qPCR conditions Quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the same standard as qPCR. .. Ten µl of enzyme mix including the SuperScriptIII® Reverse Transciptase (Invitrogen) were added to the RNA mixture and subjected to 3 steps: 25°C for 10 min, 45°C for 90 min and 9°C for 5 min. cDNA obtained was added to 10 µl of SYBR Green buffer and 150 nM of each GF5qpcr and GR5qpcr primers in a final volume of 20 µl.

    Sequencing:

    Article Title: Tuning Piezo ion channels to detect molecular-scale movements relevant for fine touch
    Article Snippet: Two micrograms of RNA per reaction was reverse transcribed with SuperscriptIII Reverse Transciptase (Invitrogen) using random hexamers. .. With this cDNA, qPCR reactions were performed on a 7900Abi Sequence Detection System (Applied Biosystems) using probes from the Universal Probe Library (Roche).

    Positive Control:

    Article Title: Viral Latency in Blood and Saliva of Simian Foamy Virus-Infected Humans
    Article Snippet: Ten µl of enzyme mix including the SuperScriptIII® Reverse Transciptase (Invitrogen) were added to the RNA mixture and subjected to 3 steps: 25°C for 10 min, 45°C for 90 min and 9°C for 5 min. cDNA obtained was added to 10 µl of SYBR Green buffer and 150 nM of each GF5qpcr and GR5qpcr primers in a final volume of 20 µl. .. Our positive control was RNA extracted from the human microglial cell line CHME productively infected with SFV strain from BAK74.

    Spectrophotometry:

    Article Title: Tuning Piezo ion channels to detect molecular-scale movements relevant for fine touch
    Article Snippet: RNA was quantified using a NanoDrop 2000 Spectrophotometer (Thermo Scientific). .. Two micrograms of RNA per reaction was reverse transcribed with SuperscriptIII Reverse Transciptase (Invitrogen) using random hexamers.

    SYBR Green Assay:

    Article Title: Viral Latency in Blood and Saliva of Simian Foamy Virus-Infected Humans
    Article Snippet: .. Ten µl of enzyme mix including the SuperScriptIII® Reverse Transciptase (Invitrogen) were added to the RNA mixture and subjected to 3 steps: 25°C for 10 min, 45°C for 90 min and 9°C for 5 min. cDNA obtained was added to 10 µl of SYBR Green buffer and 150 nM of each GF5qpcr and GR5qpcr primers in a final volume of 20 µl. ..

    Polymerase Chain Reaction:

    Article Title: Tuning Piezo ion channels to detect molecular-scale movements relevant for fine touch
    Article Snippet: Two micrograms of RNA per reaction was reverse transcribed with SuperscriptIII Reverse Transciptase (Invitrogen) using random hexamers. .. All PCR reactions were conducted in triplicate.

    Random Hexamer Labeling:

    Article Title: Viral Latency in Blood and Saliva of Simian Foamy Virus-Infected Humans
    Article Snippet: RNA samples quantified by ND 8000 (Nanodrop Technologies) were mixed with random hexamer primers (5µM final), dNTP (1mM final) and completed with DEPC-treated water until a final volume of 10 µl. .. Ten µl of enzyme mix including the SuperScriptIII® Reverse Transciptase (Invitrogen) were added to the RNA mixture and subjected to 3 steps: 25°C for 10 min, 45°C for 90 min and 9°C for 5 min. cDNA obtained was added to 10 µl of SYBR Green buffer and 150 nM of each GF5qpcr and GR5qpcr primers in a final volume of 20 µl.

    Infection:

    Article Title: Viral Latency in Blood and Saliva of Simian Foamy Virus-Infected Humans
    Article Snippet: Ten µl of enzyme mix including the SuperScriptIII® Reverse Transciptase (Invitrogen) were added to the RNA mixture and subjected to 3 steps: 25°C for 10 min, 45°C for 90 min and 9°C for 5 min. cDNA obtained was added to 10 µl of SYBR Green buffer and 150 nM of each GF5qpcr and GR5qpcr primers in a final volume of 20 µl. .. Our positive control was RNA extracted from the human microglial cell line CHME productively infected with SFV strain from BAK74.

    Quantitative RT-PCR:

    Article Title: Viral Latency in Blood and Saliva of Simian Foamy Virus-Infected Humans
    Article Snippet: Paragraph title: RT-qPCR conditions ... Ten µl of enzyme mix including the SuperScriptIII® Reverse Transciptase (Invitrogen) were added to the RNA mixture and subjected to 3 steps: 25°C for 10 min, 45°C for 90 min and 9°C for 5 min. cDNA obtained was added to 10 µl of SYBR Green buffer and 150 nM of each GF5qpcr and GR5qpcr primers in a final volume of 20 µl.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Viral Latency in Blood and Saliva of Simian Foamy Virus-Infected Humans
    Article Snippet: RT-qPCR conditions Quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the same standard as qPCR. .. Ten µl of enzyme mix including the SuperScriptIII® Reverse Transciptase (Invitrogen) were added to the RNA mixture and subjected to 3 steps: 25°C for 10 min, 45°C for 90 min and 9°C for 5 min. cDNA obtained was added to 10 µl of SYBR Green buffer and 150 nM of each GF5qpcr and GR5qpcr primers in a final volume of 20 µl.

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  • 99
    Thermo Fisher superscriptiii reverse transcriptase
    Superscriptiii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiii reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 158 article reviews
    Price from $9.99 to $1999.99
    superscriptiii reverse transcriptase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    96
    Thermo Fisher superscriptiv reverse transcriptase
    Superscriptiv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiv reverse transcriptase/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscriptiv reverse transcriptase - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

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