superscriptiii first strand synthesis system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscriptiii first strand synthesis system
    Superscriptiii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiii first strand synthesis system/product/Thermo Fisher
    Average 97 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    superscriptiii first strand synthesis system - by Bioz Stars, 2020-04
    97/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Loss of DUX causes minor defects in zygotic genome activation and is compatible with mouse development
    Article Snippet: Paragraph title: RNA isolation, reverse transcription, and quantitative PCR ... Following RQ1 DNase (Promega) treatment, RNA was used as template to synthesize cDNA with the use of SuperScriptIII First-Strand Synthesis System (Invitrogen).

    Article Title: MicroRNA-Based Separation of Cortico-Fugal Projection Neuron-Like Cells Derived From Embryonic Stem Cells
    Article Snippet: .. Reverse Transcription and Quantitative PCR To detect the mRNA transcripts, purified RNA was reverse transcribed using the SuperScriptIII First-Strand Synthesis System (Invitrogen). .. Quantitative PCR reactions were carried out with Power SYBR (Applied Biosystems) according to the manufacturer’s instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Chronic Ultraviolet B Irradiation Causes Loss of Hyaluronic Acid from Mouse Dermis Because of Down-Regulation of Hyaluronic Acid Synthases
    Article Snippet: .. Aliquots of total RNA (100 ng) were applied for cDNA synthesis using SuperscriptIII first-strand synthesis system for reverse transcriptase-polymerase chain reaction (RT-PCR) (Invitrogen, Karlsruhe, Germany). ..

    Isolation:

    Article Title: Chronic Ultraviolet B Irradiation Causes Loss of Hyaluronic Acid from Mouse Dermis Because of Down-Regulation of Hyaluronic Acid Synthases
    Article Snippet: Paragraph title: RNA Isolation and Quantification of Gene Expression ... Aliquots of total RNA (100 ng) were applied for cDNA synthesis using SuperscriptIII first-strand synthesis system for reverse transcriptase-polymerase chain reaction (RT-PCR) (Invitrogen, Karlsruhe, Germany).

    Article Title: Loss of DUX causes minor defects in zygotic genome activation and is compatible with mouse development
    Article Snippet: Paragraph title: RNA isolation, reverse transcription, and quantitative PCR ... Following RQ1 DNase (Promega) treatment, RNA was used as template to synthesize cDNA with the use of SuperScriptIII First-Strand Synthesis System (Invitrogen).

    Purification:

    Article Title: MicroRNA-Based Separation of Cortico-Fugal Projection Neuron-Like Cells Derived From Embryonic Stem Cells
    Article Snippet: .. Reverse Transcription and Quantitative PCR To detect the mRNA transcripts, purified RNA was reverse transcribed using the SuperScriptIII First-Strand Synthesis System (Invitrogen). .. Quantitative PCR reactions were carried out with Power SYBR (Applied Biosystems) according to the manufacturer’s instructions.

    SYBR Green Assay:

    Article Title: Loss of DUX causes minor defects in zygotic genome activation and is compatible with mouse development
    Article Snippet: Following RQ1 DNase (Promega) treatment, RNA was used as template to synthesize cDNA with the use of SuperScriptIII First-Strand Synthesis System (Invitrogen). .. SYBR green gene expression assay (Invitrogen) were used to determine Dux transcript abundance in a ViiA 7 Real-Time PCR System (ThermoFisher Scientific).

    Concentration Assay:

    Article Title: Chronic Ultraviolet B Irradiation Causes Loss of Hyaluronic Acid from Mouse Dermis Because of Down-Regulation of Hyaluronic Acid Synthases
    Article Snippet: The RNA concentration was determined via photometric measurement at 260/280. .. Aliquots of total RNA (100 ng) were applied for cDNA synthesis using SuperscriptIII first-strand synthesis system for reverse transcriptase-polymerase chain reaction (RT-PCR) (Invitrogen, Karlsruhe, Germany).

    Expressing:

    Article Title: Chronic Ultraviolet B Irradiation Causes Loss of Hyaluronic Acid from Mouse Dermis Because of Down-Regulation of Hyaluronic Acid Synthases
    Article Snippet: Paragraph title: RNA Isolation and Quantification of Gene Expression ... Aliquots of total RNA (100 ng) were applied for cDNA synthesis using SuperscriptIII first-strand synthesis system for reverse transcriptase-polymerase chain reaction (RT-PCR) (Invitrogen, Karlsruhe, Germany).

    Article Title: Loss of DUX causes minor defects in zygotic genome activation and is compatible with mouse development
    Article Snippet: Following RQ1 DNase (Promega) treatment, RNA was used as template to synthesize cDNA with the use of SuperScriptIII First-Strand Synthesis System (Invitrogen). .. SYBR green gene expression assay (Invitrogen) were used to determine Dux transcript abundance in a ViiA 7 Real-Time PCR System (ThermoFisher Scientific).

    Article Title: MicroRNA-Based Separation of Cortico-Fugal Projection Neuron-Like Cells Derived From Embryonic Stem Cells
    Article Snippet: Reverse Transcription and Quantitative PCR To detect the mRNA transcripts, purified RNA was reverse transcribed using the SuperScriptIII First-Strand Synthesis System (Invitrogen). .. The expression level of each gene was normalized to that of GAPDH using the ΔΔ-Ct method.

    Sequencing:

    Article Title: Chronic Ultraviolet B Irradiation Causes Loss of Hyaluronic Acid from Mouse Dermis Because of Down-Regulation of Hyaluronic Acid Synthases
    Article Snippet: Aliquots of total RNA (100 ng) were applied for cDNA synthesis using SuperscriptIII first-strand synthesis system for reverse transcriptase-polymerase chain reaction (RT-PCR) (Invitrogen, Karlsruhe, Germany). .. For analysis of mRNA expression in human fibroblasts, a specific primer pair was designed by Primer Express 2.0 software (Applied Biosystems, Darmstadt, Germany) based on the cDNA sequence published as indicated.

    Software:

    Article Title: Chronic Ultraviolet B Irradiation Causes Loss of Hyaluronic Acid from Mouse Dermis Because of Down-Regulation of Hyaluronic Acid Synthases
    Article Snippet: Aliquots of total RNA (100 ng) were applied for cDNA synthesis using SuperscriptIII first-strand synthesis system for reverse transcriptase-polymerase chain reaction (RT-PCR) (Invitrogen, Karlsruhe, Germany). .. For analysis of mRNA expression in human fibroblasts, a specific primer pair was designed by Primer Express 2.0 software (Applied Biosystems, Darmstadt, Germany) based on the cDNA sequence published as indicated.

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    Thermo Fisher superscript iii first strand synthesis system
    Superscript Iii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 739 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii first strand synthesis system/product/Thermo Fisher
    Average 99 stars, based on 739 article reviews
    Price from $9.99 to $1999.99
    superscript iii first strand synthesis system - by Bioz Stars, 2020-04
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    Thermo Fisher superscript iv first strand synthesis system
    Superscript Iv First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iv first strand synthesis system/product/Thermo Fisher
    Average 99 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    superscript iv first strand synthesis system - by Bioz Stars, 2020-04
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    Thermo Fisher superscriptiii first strand cdna synthesis kit
    Superscriptiii First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiii first strand cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscriptiii first strand cdna synthesis kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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