superscriptii rnase h reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscriptii rnase h reverse transcriptase
    Superscriptii Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptii rnase h reverse transcriptase/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscriptii rnase h reverse transcriptase - by Bioz Stars, 2020-02
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Targeting KV channels rescues retinal ganglion cells in vivo directly and by reducing inflammation
    Article Snippet: Total RNA (2 µg) was reverse transcribed in 20 µL volume using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 mM dNTPs (Invitrogen, Carlsbad, CA) and 0.5 µM oligo dT (Sigma-Aldrich, St. Louis, MO). .. Amplification was performed on an ABI PRISM 7900 Sequence Detection System (PE Biosystems, Foster City, CA) at 95°C for 10 min, followed by 40 cycles at 95°C for 15 s, 55°C for 15 s and 72°C for 30 s. For each gene, ‘no-template’ and ‘no-amplification’ controls were negative, and melt curves showed a single peak, confirming specific amplification.

    Article Title: The Ca2+-Activated K+ Channel KCNN4/KCa3.1 Contributes to Microglia Activation and Nitric Oxide-Dependent Neurodegeneration
    Article Snippet: A two-step reaction was performed according to the instructions of the manufacturer (Invitrogen): total RNA (2 μg) was reverse transcribed using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 m m dNTPs (Invitrogen) and 0.5 μ m oligo-dT (Sigma). .. Amplification was performed on an ABI PRISM 7700 Sequence Detection System (PE Biosystems, Foster City, CA) at 95°C for 10 min, followed by 40 cycles at 95°C for 15 s, 55°C for 15 s, and 72°C for 30 s. “No-template” and “no-amplification” controls ( ) were included for each gene.

    Isolation:

    Article Title: Targeting KV channels rescues retinal ganglion cells in vivo directly and by reducing inflammation
    Article Snippet: RNA was isolated (RNeasy; Qiagen, Inc., Mississauga, Ontario) after degrading any contaminating DNA with DNaseI (0.1 U/mL, 15 min, 37°C; Amersham Biosciences, Baie d'Urfe, Quebec). .. Total RNA (2 µg) was reverse transcribed in 20 µL volume using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 mM dNTPs (Invitrogen, Carlsbad, CA) and 0.5 µM oligo dT (Sigma-Aldrich, St. Louis, MO).

    Microscopy:

    Article Title: The Ca2+-Activated K+ Channel KCNN4/KCa3.1 Contributes to Microglia Activation and Nitric Oxide-Dependent Neurodegeneration
    Article Snippet: The neural retina was dissected, flat mounted in 1:1 glycerol/PBS, viewed at room temperature with a Zeiss LSM 510 confocal microscope using a 20× quartz objective, and analyzed with Zeiss LSM 510 software version 3.2 SP2 software provided with the microscope. .. A two-step reaction was performed according to the instructions of the manufacturer (Invitrogen): total RNA (2 μg) was reverse transcribed using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 m m dNTPs (Invitrogen) and 0.5 μ m oligo-dT (Sigma).

    Cell Counting:

    Article Title: The Ca2+-Activated K+ Channel KCNN4/KCa3.1 Contributes to Microglia Activation and Nitric Oxide-Dependent Neurodegeneration
    Article Snippet: Paragraph title: Optic nerve transection, drug injection, and cell counting. ... A two-step reaction was performed according to the instructions of the manufacturer (Invitrogen): total RNA (2 μg) was reverse transcribed using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 m m dNTPs (Invitrogen) and 0.5 μ m oligo-dT (Sigma).

    Quantitative RT-PCR:

    Article Title: Targeting KV channels rescues retinal ganglion cells in vivo directly and by reducing inflammation
    Article Snippet: Paragraph title: Real-time quantitative RT-PCR. ... Total RNA (2 µg) was reverse transcribed in 20 µL volume using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 mM dNTPs (Invitrogen, Carlsbad, CA) and 0.5 µM oligo dT (Sigma-Aldrich, St. Louis, MO).

    Expressing:

    Article Title: Targeting KV channels rescues retinal ganglion cells in vivo directly and by reducing inflammation
    Article Snippet: Changes in gene transcript expression were monitored by quantitative RT-PCR (qRT-PCR) in whole retinas harvested seven days after axotomy, as before.). .. Total RNA (2 µg) was reverse transcribed in 20 µL volume using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 mM dNTPs (Invitrogen, Carlsbad, CA) and 0.5 µM oligo dT (Sigma-Aldrich, St. Louis, MO).

    Sequencing:

    Article Title: Targeting KV channels rescues retinal ganglion cells in vivo directly and by reducing inflammation
    Article Snippet: Total RNA (2 µg) was reverse transcribed in 20 µL volume using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 mM dNTPs (Invitrogen, Carlsbad, CA) and 0.5 µM oligo dT (Sigma-Aldrich, St. Louis, MO). .. Amplification was performed on an ABI PRISM 7900 Sequence Detection System (PE Biosystems, Foster City, CA) at 95°C for 10 min, followed by 40 cycles at 95°C for 15 s, 55°C for 15 s and 72°C for 30 s. For each gene, ‘no-template’ and ‘no-amplification’ controls were negative, and melt curves showed a single peak, confirming specific amplification.

    Article Title: The Ca2+-Activated K+ Channel KCNN4/KCa3.1 Contributes to Microglia Activation and Nitric Oxide-Dependent Neurodegeneration
    Article Snippet: A two-step reaction was performed according to the instructions of the manufacturer (Invitrogen): total RNA (2 μg) was reverse transcribed using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 m m dNTPs (Invitrogen) and 0.5 μ m oligo-dT (Sigma). .. Amplification was performed on an ABI PRISM 7700 Sequence Detection System (PE Biosystems, Foster City, CA) at 95°C for 10 min, followed by 40 cycles at 95°C for 15 s, 55°C for 15 s, and 72°C for 30 s. “No-template” and “no-amplification” controls ( ) were included for each gene.

    Injection:

    Article Title: The Ca2+-Activated K+ Channel KCNN4/KCa3.1 Contributes to Microglia Activation and Nitric Oxide-Dependent Neurodegeneration
    Article Snippet: Paragraph title: Optic nerve transection, drug injection, and cell counting. ... A two-step reaction was performed according to the instructions of the manufacturer (Invitrogen): total RNA (2 μg) was reverse transcribed using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 m m dNTPs (Invitrogen) and 0.5 μ m oligo-dT (Sigma).

    Binding Assay:

    Article Title: The Ca2+-Activated K+ Channel KCNN4/KCa3.1 Contributes to Microglia Activation and Nitric Oxide-Dependent Neurodegeneration
    Article Snippet: A two-step reaction was performed according to the instructions of the manufacturer (Invitrogen): total RNA (2 μg) was reverse transcribed using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 m m dNTPs (Invitrogen) and 0.5 μ m oligo-dT (Sigma). .. Relative input RNA amounts were determined from a relative standard curve for each gene of interest and the housekeeping gene TATA-box binding protein .

    Software:

    Article Title: The Ca2+-Activated K+ Channel KCNN4/KCa3.1 Contributes to Microglia Activation and Nitric Oxide-Dependent Neurodegeneration
    Article Snippet: The neural retina was dissected, flat mounted in 1:1 glycerol/PBS, viewed at room temperature with a Zeiss LSM 510 confocal microscope using a 20× quartz objective, and analyzed with Zeiss LSM 510 software version 3.2 SP2 software provided with the microscope. .. A two-step reaction was performed according to the instructions of the manufacturer (Invitrogen): total RNA (2 μg) was reverse transcribed using 200 U of SuperScriptII RNase H-reverse transcriptase, with 0.5 m m dNTPs (Invitrogen) and 0.5 μ m oligo-dT (Sigma).

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  • 90
    Thermo Fisher superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 589 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii reverse transcriptase/product/Thermo Fisher
    Average 90 stars, based on 589 article reviews
    Price from $9.99 to $1999.99
    superscript ii reverse transcriptase - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher superscript iii rnase h reverse transcription
    Superscript Iii Rnase H Reverse Transcription, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii rnase h reverse transcription/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscript iii rnase h reverse transcription - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

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