superscriptase ii moloney murine leukemia virus reverse transcriptase  (Thermo Fisher)


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    Name:
    SuperScript II Reverse Transcriptase
    Description:
    Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase RT with reduced RNase H activity and increased thermal stability compared to wild type MMLV RT Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first strand cDNA synthesis which results in higher yields of full length cDNA SuperScript RTs are the most highly trusted and widely used RTs with over 50 000 citations reviews and publications to date Note The latest member of the SuperScript RT family SuperScript IV Reverse Transcriptase features enhanced thermostability processivity yields and performance with any RNA samples including those of suboptimal purity or integrity
    Catalog Number:
    18064014
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher superscriptase ii moloney murine leukemia virus reverse transcriptase
    Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase RT with reduced RNase H activity and increased thermal stability compared to wild type MMLV RT Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first strand cDNA synthesis which results in higher yields of full length cDNA SuperScript RTs are the most highly trusted and widely used RTs with over 50 000 citations reviews and publications to date Note The latest member of the SuperScript RT family SuperScript IV Reverse Transcriptase features enhanced thermostability processivity yields and performance with any RNA samples including those of suboptimal purity or integrity
    https://www.bioz.com/result/superscriptase ii moloney murine leukemia virus reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscriptase ii moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-12
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells
    Article Snippet: .. Cloning of transcribed L1Rn sequences from RCL cells Total and poly(A)+ RNA were prepared from control and irradiated RCL cells with the RNeasy and Oligotex mRNA kits (Qiagen), respectively. cDNA was synthesized from poly(A)+ RNA employing SuperScript™ II reverse transcriptase (Invitrogen) and oligo(dT) primer. .. About 6 kb long L1Rn sequences encompassing ORF1 and ORF2 were amplified from cDNA with the Taq/Pwo Proof Mix (AGS-Hybaid) and primers Pr-L1Rn1 (5′-GGA AGA GAC CAC CAA CAC TGC TCA C-3′, pos.

    Article Title: Structure and expression of two nuclear receptor genes in marsupials: insights into the evolution of the antisense overlap between the ?-thyroid hormone receptor and Rev-erb?
    Article Snippet: .. Nucleic acid sequencing and cloning DNA was sequenced from the overlap regions spanning the TRα and Rev-erbα genes of Didelphis virginiana and Potorous tridactylus following amplification of total DNA using primers complementary to conserved sites within exon 9 of TRα1 and exons 7 and 8 of Rev-erbα. mRNA sequencing was similarly carried out with cDNA obtained by randomly primed reverse transcription of total RNA (Superscript II; Invitrogen) following homogenization of tissue in TRIzol according to manufacturer's instructions (Invitrogen). .. Amplified genomic products were also cloned into pGEM-T vector (Promega; Madison, WI) and subcloned into pErbA construct as previously described [ ].

    Amplification:

    Article Title: Corky, a gypsy-like retrotransposon is differentially transcribed in Quercus suber tissues
    Article Snippet: .. RNA samples were treated with RQ1 RNase-Free DNase (Promega, Madison, WI). cDNA was synthesized from 2 μg of total RNA using random hexamers and Superscript II RNase H- reverse transcriptase (Invitrogen®, Carlsbad, CA), according to the manufacturer’s recommendations followed by PCR amplification using specific primers for the RVT and a region between Integrase and the chromodomain of Corky (Figure ). .. As expected, amplification products were not obtained in RNA samples not yielded to reverse transcription prior to PCR. cDNA was stored at −20°C.

    Article Title: Structure and expression of two nuclear receptor genes in marsupials: insights into the evolution of the antisense overlap between the ?-thyroid hormone receptor and Rev-erb?
    Article Snippet: .. Nucleic acid sequencing and cloning DNA was sequenced from the overlap regions spanning the TRα and Rev-erbα genes of Didelphis virginiana and Potorous tridactylus following amplification of total DNA using primers complementary to conserved sites within exon 9 of TRα1 and exons 7 and 8 of Rev-erbα. mRNA sequencing was similarly carried out with cDNA obtained by randomly primed reverse transcription of total RNA (Superscript II; Invitrogen) following homogenization of tissue in TRIzol according to manufacturer's instructions (Invitrogen). .. Amplified genomic products were also cloned into pGEM-T vector (Promega; Madison, WI) and subcloned into pErbA construct as previously described [ ].

    Homogenization:

    Article Title: Structure and expression of two nuclear receptor genes in marsupials: insights into the evolution of the antisense overlap between the ?-thyroid hormone receptor and Rev-erb?
    Article Snippet: .. Nucleic acid sequencing and cloning DNA was sequenced from the overlap regions spanning the TRα and Rev-erbα genes of Didelphis virginiana and Potorous tridactylus following amplification of total DNA using primers complementary to conserved sites within exon 9 of TRα1 and exons 7 and 8 of Rev-erbα. mRNA sequencing was similarly carried out with cDNA obtained by randomly primed reverse transcription of total RNA (Superscript II; Invitrogen) following homogenization of tissue in TRIzol according to manufacturer's instructions (Invitrogen). .. Amplified genomic products were also cloned into pGEM-T vector (Promega; Madison, WI) and subcloned into pErbA construct as previously described [ ].

    Isolation:

    Article Title: Mef2c is an essential regulatory element required for unique expression of the cardiac-specific CARK gene
    Article Snippet: .. Total RNAs were isolated using Trizol reagent (Invitrogen) and treated with DNase I (Ambion) according to the manufacturer's instructions. cDNAs were generated using oligo(dT)12-18 as a primer and SuperScript II RT-PCR kit (Invitrogen).cDNA was added to 25 μl of reaction volume containing 12.5 μl of 2× SYBR Green Premix EX Taq (TaKaRa, Otsu, Japan), and 400 nmol/L CARK gene-specific primers (forward: 5′-GACAAAGCAGCCAGGGAA; reverse: 5′-AGCAGGCAATCGGAAGAA). .. To confirm amplification specificity, the PCR products were subjected to a melting curve analysis.

    Synthesized:

    Article Title: Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells
    Article Snippet: .. Cloning of transcribed L1Rn sequences from RCL cells Total and poly(A)+ RNA were prepared from control and irradiated RCL cells with the RNeasy and Oligotex mRNA kits (Qiagen), respectively. cDNA was synthesized from poly(A)+ RNA employing SuperScript™ II reverse transcriptase (Invitrogen) and oligo(dT) primer. .. About 6 kb long L1Rn sequences encompassing ORF1 and ORF2 were amplified from cDNA with the Taq/Pwo Proof Mix (AGS-Hybaid) and primers Pr-L1Rn1 (5′-GGA AGA GAC CAC CAA CAC TGC TCA C-3′, pos.

    Article Title: Corky, a gypsy-like retrotransposon is differentially transcribed in Quercus suber tissues
    Article Snippet: .. RNA samples were treated with RQ1 RNase-Free DNase (Promega, Madison, WI). cDNA was synthesized from 2 μg of total RNA using random hexamers and Superscript II RNase H- reverse transcriptase (Invitrogen®, Carlsbad, CA), according to the manufacturer’s recommendations followed by PCR amplification using specific primers for the RVT and a region between Integrase and the chromodomain of Corky (Figure ). .. As expected, amplification products were not obtained in RNA samples not yielded to reverse transcription prior to PCR. cDNA was stored at −20°C.

    Irradiation:

    Article Title: Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells
    Article Snippet: .. Cloning of transcribed L1Rn sequences from RCL cells Total and poly(A)+ RNA were prepared from control and irradiated RCL cells with the RNeasy and Oligotex mRNA kits (Qiagen), respectively. cDNA was synthesized from poly(A)+ RNA employing SuperScript™ II reverse transcriptase (Invitrogen) and oligo(dT) primer. .. About 6 kb long L1Rn sequences encompassing ORF1 and ORF2 were amplified from cDNA with the Taq/Pwo Proof Mix (AGS-Hybaid) and primers Pr-L1Rn1 (5′-GGA AGA GAC CAC CAA CAC TGC TCA C-3′, pos.

    SYBR Green Assay:

    Article Title: Mef2c is an essential regulatory element required for unique expression of the cardiac-specific CARK gene
    Article Snippet: .. Total RNAs were isolated using Trizol reagent (Invitrogen) and treated with DNase I (Ambion) according to the manufacturer's instructions. cDNAs were generated using oligo(dT)12-18 as a primer and SuperScript II RT-PCR kit (Invitrogen).cDNA was added to 25 μl of reaction volume containing 12.5 μl of 2× SYBR Green Premix EX Taq (TaKaRa, Otsu, Japan), and 400 nmol/L CARK gene-specific primers (forward: 5′-GACAAAGCAGCCAGGGAA; reverse: 5′-AGCAGGCAATCGGAAGAA). .. To confirm amplification specificity, the PCR products were subjected to a melting curve analysis.

    Polymerase Chain Reaction:

    Article Title: Corky, a gypsy-like retrotransposon is differentially transcribed in Quercus suber tissues
    Article Snippet: .. RNA samples were treated with RQ1 RNase-Free DNase (Promega, Madison, WI). cDNA was synthesized from 2 μg of total RNA using random hexamers and Superscript II RNase H- reverse transcriptase (Invitrogen®, Carlsbad, CA), according to the manufacturer’s recommendations followed by PCR amplification using specific primers for the RVT and a region between Integrase and the chromodomain of Corky (Figure ). .. As expected, amplification products were not obtained in RNA samples not yielded to reverse transcription prior to PCR. cDNA was stored at −20°C.

    Article Title: Developmental arrest of T cells in RpL22-deficient mice is dependent upon multiple p53 effectors 1
    Article Snippet: .. Purified mRNA was converted to cDNA using Superscript II reverse transcription PCR with oligo dT(12–18) primers (Invitrogen, Carlsbad, CA). .. Quantitative real-time PCR was performed on the Prism 7700 thermocycler (Applied Biosystems, Carlsbad, CA) using Taqman Real-time PCR primer/probe sets specific to murine PUMA/ Bbc3 (Mm00519268_m1), Noxa/ Pmaip1 (Mm00451763_m1), and Bim/( Bcl2l-11 ) (Mm01333921_m1).

    Generated:

    Article Title: Mef2c is an essential regulatory element required for unique expression of the cardiac-specific CARK gene
    Article Snippet: .. Total RNAs were isolated using Trizol reagent (Invitrogen) and treated with DNase I (Ambion) according to the manufacturer's instructions. cDNAs were generated using oligo(dT)12-18 as a primer and SuperScript II RT-PCR kit (Invitrogen).cDNA was added to 25 μl of reaction volume containing 12.5 μl of 2× SYBR Green Premix EX Taq (TaKaRa, Otsu, Japan), and 400 nmol/L CARK gene-specific primers (forward: 5′-GACAAAGCAGCCAGGGAA; reverse: 5′-AGCAGGCAATCGGAAGAA). .. To confirm amplification specificity, the PCR products were subjected to a melting curve analysis.

    Sequencing:

    Article Title: Structure and expression of two nuclear receptor genes in marsupials: insights into the evolution of the antisense overlap between the ?-thyroid hormone receptor and Rev-erb?
    Article Snippet: .. Nucleic acid sequencing and cloning DNA was sequenced from the overlap regions spanning the TRα and Rev-erbα genes of Didelphis virginiana and Potorous tridactylus following amplification of total DNA using primers complementary to conserved sites within exon 9 of TRα1 and exons 7 and 8 of Rev-erbα. mRNA sequencing was similarly carried out with cDNA obtained by randomly primed reverse transcription of total RNA (Superscript II; Invitrogen) following homogenization of tissue in TRIzol according to manufacturer's instructions (Invitrogen). .. Amplified genomic products were also cloned into pGEM-T vector (Promega; Madison, WI) and subcloned into pErbA construct as previously described [ ].

    Purification:

    Article Title: Developmental arrest of T cells in RpL22-deficient mice is dependent upon multiple p53 effectors 1
    Article Snippet: .. Purified mRNA was converted to cDNA using Superscript II reverse transcription PCR with oligo dT(12–18) primers (Invitrogen, Carlsbad, CA). .. Quantitative real-time PCR was performed on the Prism 7700 thermocycler (Applied Biosystems, Carlsbad, CA) using Taqman Real-time PCR primer/probe sets specific to murine PUMA/ Bbc3 (Mm00519268_m1), Noxa/ Pmaip1 (Mm00451763_m1), and Bim/( Bcl2l-11 ) (Mm01333921_m1).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Template switching causes artificial junction formation and false identification of circular RNAs
    Article Snippet: .. RT-PCR analyses of linear and circular RNAs Cellular RNAs or synthetic control linear or circular RNAs were reverse transcribed using MonsterScript (Cat# MS041050, Epicentre) and SuperScript II (Cat# 18064014, Thermo Fisher) in a reaction containing the following reagents: 4μl 5xBuffer (supplementing 10mM DTT for SuperScript II), 1μl dNTP, 1μl reverse transcriptase, 1μl control RNA, 1μl Smarter IIA oligo (10μM), 1μl gene-specific primer (10uM), 11μl water. .. PCR was performed using GoTaq DNA polymerase (Cat# M3001, Promega) and convergent/divergent primers at the annealing temperature of 55°C.

    Article Title: Mef2c is an essential regulatory element required for unique expression of the cardiac-specific CARK gene
    Article Snippet: .. Total RNAs were isolated using Trizol reagent (Invitrogen) and treated with DNase I (Ambion) according to the manufacturer's instructions. cDNAs were generated using oligo(dT)12-18 as a primer and SuperScript II RT-PCR kit (Invitrogen).cDNA was added to 25 μl of reaction volume containing 12.5 μl of 2× SYBR Green Premix EX Taq (TaKaRa, Otsu, Japan), and 400 nmol/L CARK gene-specific primers (forward: 5′-GACAAAGCAGCCAGGGAA; reverse: 5′-AGCAGGCAATCGGAAGAA). .. To confirm amplification specificity, the PCR products were subjected to a melting curve analysis.