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GE Healthcare superscript iii reverse transcriptase
Superscript Iii Reverse Transcriptase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscript iii reverse transcriptase/product/GE Healthcare
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
superscript iii reverse transcriptase - by Bioz Stars, 2020-04
85/100 stars

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Agarose Gel Electrophoresis:

Article Title: Antisense morpholino targeting just upstream from a poly(A) tail junction of maternal mRNA removes the tail and inhibits translation
Article Snippet: After incubation for 1 h at 42°C with T4 DNA ligase (350 U) (TaKaRa), the samples were further incubated at 12°C for 1 h in the presence of 200 ng (dT)12 -anchor primer (5′-GCGAGCTCCG CGGCCGCGTT TTTTTTTTTT-3′) to generate the 3′-end of the poly (dT) primer, followed by incubation with SuperScript III reverse transcriptase (200 U) (GE Healthcare) for 1 h to make PAT complementary DNAs (cDNAs). .. Finally, the products of PCR using a gene-specific primer and the (dT)12 -anchor primer were subjected to 2.2% agarose gel electrophoresis and visualized by ethidium bromide.

Labeling:

Article Title: Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I?C)
Article Snippet: Paragraph title: RT and direct method for dye labeling. ... Samples were incubated at 70°C for 10 min in a dry bath to reduce secondary structure formation and chilled on ice for 5 min. RT of RNA into cDNA was performed by using a SuperScript III reverse transcriptase kit (Invitrogen) by mixing 5× strand buffer, 0.1 M dithiothreitol, 2 mM dCTP, 6.67 mM dATP, 6.67 mM dGTP, 6.67 mM dTTP, 200 units of SuperScript III reverse transcriptase, and 1 mM Cy3-dCTP or Cy5-dCTP dye (GE Healthcare Amersham Biosciences) in a total volume of 20 μl.

Purification:

Article Title: Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I?C)
Article Snippet: Samples were incubated at 70°C for 10 min in a dry bath to reduce secondary structure formation and chilled on ice for 5 min. RT of RNA into cDNA was performed by using a SuperScript III reverse transcriptase kit (Invitrogen) by mixing 5× strand buffer, 0.1 M dithiothreitol, 2 mM dCTP, 6.67 mM dATP, 6.67 mM dGTP, 6.67 mM dTTP, 200 units of SuperScript III reverse transcriptase, and 1 mM Cy3-dCTP or Cy5-dCTP dye (GE Healthcare Amersham Biosciences) in a total volume of 20 μl. .. Reverse-transcribed and dye-labeled samples of cDNA were column purified using a QIAquick PCR purification kit per the manufacturer's instructions (Qiagen).

Incubation:

Article Title: Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I?C)
Article Snippet: .. Samples were incubated at 70°C for 10 min in a dry bath to reduce secondary structure formation and chilled on ice for 5 min. RT of RNA into cDNA was performed by using a SuperScript III reverse transcriptase kit (Invitrogen) by mixing 5× strand buffer, 0.1 M dithiothreitol, 2 mM dCTP, 6.67 mM dATP, 6.67 mM dGTP, 6.67 mM dTTP, 200 units of SuperScript III reverse transcriptase, and 1 mM Cy3-dCTP or Cy5-dCTP dye (GE Healthcare Amersham Biosciences) in a total volume of 20 μl. .. Samples were incubated at 42°C for 3 h (dry bath) to allow RT, and samples were kept in the dark to avoid photobleaching of the dyes.

Article Title: Antisense morpholino targeting just upstream from a poly(A) tail junction of maternal mRNA removes the tail and inhibits translation
Article Snippet: .. After incubation for 1 h at 42°C with T4 DNA ligase (350 U) (TaKaRa), the samples were further incubated at 12°C for 1 h in the presence of 200 ng (dT)12 -anchor primer (5′-GCGAGCTCCG CGGCCGCGTT TTTTTTTTTT-3′) to generate the 3′-end of the poly (dT) primer, followed by incubation with SuperScript III reverse transcriptase (200 U) (GE Healthcare) for 1 h to make PAT complementary DNAs (cDNAs). .. Finally, the products of PCR using a gene-specific primer and the (dT)12 -anchor primer were subjected to 2.2% agarose gel electrophoresis and visualized by ethidium bromide.

Polymerase Chain Reaction:

Article Title: Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I?C)
Article Snippet: Samples were incubated at 70°C for 10 min in a dry bath to reduce secondary structure formation and chilled on ice for 5 min. RT of RNA into cDNA was performed by using a SuperScript III reverse transcriptase kit (Invitrogen) by mixing 5× strand buffer, 0.1 M dithiothreitol, 2 mM dCTP, 6.67 mM dATP, 6.67 mM dGTP, 6.67 mM dTTP, 200 units of SuperScript III reverse transcriptase, and 1 mM Cy3-dCTP or Cy5-dCTP dye (GE Healthcare Amersham Biosciences) in a total volume of 20 μl. .. Reverse-transcribed and dye-labeled samples of cDNA were column purified using a QIAquick PCR purification kit per the manufacturer's instructions (Qiagen).

Article Title: Antisense morpholino targeting just upstream from a poly(A) tail junction of maternal mRNA removes the tail and inhibits translation
Article Snippet: After incubation for 1 h at 42°C with T4 DNA ligase (350 U) (TaKaRa), the samples were further incubated at 12°C for 1 h in the presence of 200 ng (dT)12 -anchor primer (5′-GCGAGCTCCG CGGCCGCGTT TTTTTTTTTT-3′) to generate the 3′-end of the poly (dT) primer, followed by incubation with SuperScript III reverse transcriptase (200 U) (GE Healthcare) for 1 h to make PAT complementary DNAs (cDNAs). .. Finally, the products of PCR using a gene-specific primer and the (dT)12 -anchor primer were subjected to 2.2% agarose gel electrophoresis and visualized by ethidium bromide.

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    GE Healthcare superscript iii reverse transcriptase
    Superscript Iii Reverse Transcriptase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase/product/GE Healthcare
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    86
    GE Healthcare superscript iii rnase h reverse transcriptase enzyme
    Superscript Iii Rnase H Reverse Transcriptase Enzyme, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii rnase h reverse transcriptase enzyme/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscript iii rnase h reverse transcriptase enzyme - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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