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Epicentre Biotechnologies superscript iii reverse transcriptase
Superscript Iii Reverse Transcriptase, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscript iii reverse transcriptase/product/Epicentre Biotechnologies
Average 87 stars, based on 2 article reviews
Price from $9.99 to $1999.99
superscript iii reverse transcriptase - by Bioz Stars, 2020-04
87/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice
Article Snippet: First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers. .. Messenger RNA levels of ATP-binding cassette A1 (ABCA1), ABCG1, ApoE, low-density lipoprotein receptor, perilipin-1 (PLIN1), PLIN2, adipose tissue lipase, HSL, PPARalpha, PGC-1alpha, CPT1, UCP2 and L32 were measured by real-time reverse transcriptase-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA, USA) and SYBR Green Master Mix (Bioline USA Inc., Tauton, MA, USA).

Article Title: ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction [S]
Article Snippet: .. Total RNA was extracted from adipose tissue or cells using TRIzol Reagent (Invitrogen, Carlsbad, CA). cDNA was synthesized using Superscript III reverse transcriptase (Epicentre Biotechnologies, Madison, WI) and random hexamer primers. mRNA levels of mouse Nos2 (nitric oxide synthase 2) , Chil3 (chitinase-like 3), Il6, Arg1 (arginase), Abca1, Abcg1, Acat, MCP1, and L32 were measured by real-time RT-PCR using the 7500 real-time PCR System (Applied Biosystems, Foster City, CA) and SybrGreen Master Mix (Bioline USA Inc., Tauton, MA). .. PCR reactions were performed in duplicate, and the data were analyzed with the ddCt method as previously described ( ).

Synthesized:

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice
Article Snippet: .. First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers. .. Messenger RNA levels of ATP-binding cassette A1 (ABCA1), ABCG1, ApoE, low-density lipoprotein receptor, perilipin-1 (PLIN1), PLIN2, adipose tissue lipase, HSL, PPARalpha, PGC-1alpha, CPT1, UCP2 and L32 were measured by real-time reverse transcriptase-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA, USA) and SYBR Green Master Mix (Bioline USA Inc., Tauton, MA, USA).

Article Title: ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction [S]
Article Snippet: .. Total RNA was extracted from adipose tissue or cells using TRIzol Reagent (Invitrogen, Carlsbad, CA). cDNA was synthesized using Superscript III reverse transcriptase (Epicentre Biotechnologies, Madison, WI) and random hexamer primers. mRNA levels of mouse Nos2 (nitric oxide synthase 2) , Chil3 (chitinase-like 3), Il6, Arg1 (arginase), Abca1, Abcg1, Acat, MCP1, and L32 were measured by real-time RT-PCR using the 7500 real-time PCR System (Applied Biosystems, Foster City, CA) and SybrGreen Master Mix (Bioline USA Inc., Tauton, MA). .. PCR reactions were performed in duplicate, and the data were analyzed with the ddCt method as previously described ( ).

Isolation:

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice
Article Snippet: Real-time quantitative reverse transcriptase-PCR Total RNA samples were isolated from whole epididymal adipose tissue using TRIzol Reagent from Invitrogen (Carlsbad, CA, USA) and quantified by spectrometry. .. First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers.

Pyrolysis Gas Chromatography:

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice
Article Snippet: First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers. .. Messenger RNA levels of ATP-binding cassette A1 (ABCA1), ABCG1, ApoE, low-density lipoprotein receptor, perilipin-1 (PLIN1), PLIN2, adipose tissue lipase, HSL, PPARalpha, PGC-1alpha, CPT1, UCP2 and L32 were measured by real-time reverse transcriptase-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA, USA) and SYBR Green Master Mix (Bioline USA Inc., Tauton, MA, USA).

Quantitative RT-PCR:

Article Title: ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction [S]
Article Snippet: .. Total RNA was extracted from adipose tissue or cells using TRIzol Reagent (Invitrogen, Carlsbad, CA). cDNA was synthesized using Superscript III reverse transcriptase (Epicentre Biotechnologies, Madison, WI) and random hexamer primers. mRNA levels of mouse Nos2 (nitric oxide synthase 2) , Chil3 (chitinase-like 3), Il6, Arg1 (arginase), Abca1, Abcg1, Acat, MCP1, and L32 were measured by real-time RT-PCR using the 7500 real-time PCR System (Applied Biosystems, Foster City, CA) and SybrGreen Master Mix (Bioline USA Inc., Tauton, MA). .. PCR reactions were performed in duplicate, and the data were analyzed with the ddCt method as previously described ( ).

SYBR Green Assay:

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice
Article Snippet: First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers. .. Messenger RNA levels of ATP-binding cassette A1 (ABCA1), ABCG1, ApoE, low-density lipoprotein receptor, perilipin-1 (PLIN1), PLIN2, adipose tissue lipase, HSL, PPARalpha, PGC-1alpha, CPT1, UCP2 and L32 were measured by real-time reverse transcriptase-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA, USA) and SYBR Green Master Mix (Bioline USA Inc., Tauton, MA, USA).

Random Hexamer Labeling:

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice
Article Snippet: .. First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers. .. Messenger RNA levels of ATP-binding cassette A1 (ABCA1), ABCG1, ApoE, low-density lipoprotein receptor, perilipin-1 (PLIN1), PLIN2, adipose tissue lipase, HSL, PPARalpha, PGC-1alpha, CPT1, UCP2 and L32 were measured by real-time reverse transcriptase-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA, USA) and SYBR Green Master Mix (Bioline USA Inc., Tauton, MA, USA).

Article Title: ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction [S]
Article Snippet: .. Total RNA was extracted from adipose tissue or cells using TRIzol Reagent (Invitrogen, Carlsbad, CA). cDNA was synthesized using Superscript III reverse transcriptase (Epicentre Biotechnologies, Madison, WI) and random hexamer primers. mRNA levels of mouse Nos2 (nitric oxide synthase 2) , Chil3 (chitinase-like 3), Il6, Arg1 (arginase), Abca1, Abcg1, Acat, MCP1, and L32 were measured by real-time RT-PCR using the 7500 real-time PCR System (Applied Biosystems, Foster City, CA) and SybrGreen Master Mix (Bioline USA Inc., Tauton, MA). .. PCR reactions were performed in duplicate, and the data were analyzed with the ddCt method as previously described ( ).

Polymerase Chain Reaction:

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice
Article Snippet: First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers. .. PCR primers were designed using Primer Bank ( http://pga.mgh.harvard.edu/primerbank/ ) ( ).

Article Title: ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction [S]
Article Snippet: Total RNA was extracted from adipose tissue or cells using TRIzol Reagent (Invitrogen, Carlsbad, CA). cDNA was synthesized using Superscript III reverse transcriptase (Epicentre Biotechnologies, Madison, WI) and random hexamer primers. mRNA levels of mouse Nos2 (nitric oxide synthase 2) , Chil3 (chitinase-like 3), Il6, Arg1 (arginase), Abca1, Abcg1, Acat, MCP1, and L32 were measured by real-time RT-PCR using the 7500 real-time PCR System (Applied Biosystems, Foster City, CA) and SybrGreen Master Mix (Bioline USA Inc., Tauton, MA). .. PCR reactions were performed in duplicate, and the data were analyzed with the ddCt method as previously described ( ).

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    Epicentre Biotechnologies superscript iii reverse transcriptase
    Superscript Iii Reverse Transcriptase, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase/product/Epicentre Biotechnologies
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase - by Bioz Stars, 2020-04
    87/100 stars
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