Structured Review

Beijing CWBio superscript iii reverse transcriptase
Superscript Iii Reverse Transcriptase, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscript iii reverse transcriptase/product/Beijing CWBio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
superscript iii reverse transcriptase - by Bioz Stars, 2020-04
86/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Bone formation in rabbit cancellous bone explant culture model is enhanced by mechanical load
Article Snippet: Paragraph title: RNA extraction and quantitative real-time PCR ... The total RNA was reversely transcribed into single-stranded cDNA using SuperRT cDNA Kit (Invitrogen), which were performed in a 20 μL reaction mixture containing 500 μM 0f dNTP Mix, 2 μl of primer mix (Life Technologies, USA) and 200 units of Superscript III reverse transcriptase according to the manufacturer’s instructions (Cwbiotech, BEI JING).

RNA Extraction:

Article Title: Bone formation in rabbit cancellous bone explant culture model is enhanced by mechanical load
Article Snippet: Paragraph title: RNA extraction and quantitative real-time PCR ... The total RNA was reversely transcribed into single-stranded cDNA using SuperRT cDNA Kit (Invitrogen), which were performed in a 20 μL reaction mixture containing 500 μM 0f dNTP Mix, 2 μl of primer mix (Life Technologies, USA) and 200 units of Superscript III reverse transcriptase according to the manufacturer’s instructions (Cwbiotech, BEI JING).

SYBR Green Assay:

Article Title: Bone formation in rabbit cancellous bone explant culture model is enhanced by mechanical load
Article Snippet: The total RNA was reversely transcribed into single-stranded cDNA using SuperRT cDNA Kit (Invitrogen), which were performed in a 20 μL reaction mixture containing 500 μM 0f dNTP Mix, 2 μl of primer mix (Life Technologies, USA) and 200 units of Superscript III reverse transcriptase according to the manufacturer’s instructions (Cwbiotech, BEI JING). .. The reaction mixture was incubated at 42°C for 60 min and at 85°C for 5 min. Quantitative real-time PCR analysis was performed with an ABI 7500 fast Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA) using a Fast SYBR-green Master Mix kit (Life Technologies, USA).

Concentration Assay:

Article Title: Bone formation in rabbit cancellous bone explant culture model is enhanced by mechanical load
Article Snippet: Concentration and purity of RNA were determined by OD 260/280 nm absorption ratio. .. The total RNA was reversely transcribed into single-stranded cDNA using SuperRT cDNA Kit (Invitrogen), which were performed in a 20 μL reaction mixture containing 500 μM 0f dNTP Mix, 2 μl of primer mix (Life Technologies, USA) and 200 units of Superscript III reverse transcriptase according to the manufacturer’s instructions (Cwbiotech, BEI JING).

Incubation:

Article Title: Bone formation in rabbit cancellous bone explant culture model is enhanced by mechanical load
Article Snippet: The total RNA was reversely transcribed into single-stranded cDNA using SuperRT cDNA Kit (Invitrogen), which were performed in a 20 μL reaction mixture containing 500 μM 0f dNTP Mix, 2 μl of primer mix (Life Technologies, USA) and 200 units of Superscript III reverse transcriptase according to the manufacturer’s instructions (Cwbiotech, BEI JING). .. The reaction mixture was incubated at 42°C for 60 min and at 85°C for 5 min. Quantitative real-time PCR analysis was performed with an ABI 7500 fast Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA) using a Fast SYBR-green Master Mix kit (Life Technologies, USA).

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    Beijing CWBio superscript iii reverse transcriptase
    Superscript Iii Reverse Transcriptase, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase/product/Beijing CWBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase - by Bioz Stars, 2020-04
    86/100 stars
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