superscript iii reverse transcriptase reagent  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript iii reverse transcriptase reagent
    Superscript Iii Reverse Transcriptase Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase reagent/product/Thermo Fisher
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase reagent - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development
    Article Snippet: Total RNA was isolated from E14 mouse tissues (tooth, skin, lung, liver, kidney, heart, eye, and brain) and from molar tooth buds at each developmental stage (E11, E13, E14, E15, and E18) using TRIzol reagent (Life Technologies) and then purified using an RNeasy Mini kit (Qiagen). cDNA was synthesized using SuperScript III reverse transcriptase reagent (Life Technologies). .. Expression of each gene was normalized to that of Gapdh . qRT-PCR was performed using iQ SYBR Green Supermix (Bio-Rad) with a CFX Connect Real-Time PCR detection system (Bio-Rad).

    Synthesized:

    Article Title: The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development
    Article Snippet: .. Total RNA was isolated from E14 mouse tissues (tooth, skin, lung, liver, kidney, heart, eye, and brain) and from molar tooth buds at each developmental stage (E11, E13, E14, E15, and E18) using TRIzol reagent (Life Technologies) and then purified using an RNeasy Mini kit (Qiagen). cDNA was synthesized using SuperScript III reverse transcriptase reagent (Life Technologies). .. The specific forward and reverse primers used for qRT-PCR were as follows: Nkx2-3 , 5′-tggccctgatgatgttacca-3′ and 5′-ggaaaactgcgtcccttcag-3′; Cdkn1a , 5′-tcccgactcttgacattgct-3′ and 5′-tccaaaatagaggggcagct-3′; Krt14 , 5′-gtacgagaagatggcggaga-3′ and 5′-ctttcatgctgagctgggac-3′; Vim , 5′-cagcagtatgaaagcgtgg-3′ and 5′-ggaagaaaaggttggcagag-3′; and glyceraldehyde 3-phosphate dehydrogenase ( Gapdh ), 5′-ggagcgagaccccactaacatc-3′ and 5′-ctcgtggttcacacccatcac-3′.

    Isolation:

    Article Title: The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development
    Article Snippet: .. Total RNA was isolated from E14 mouse tissues (tooth, skin, lung, liver, kidney, heart, eye, and brain) and from molar tooth buds at each developmental stage (E11, E13, E14, E15, and E18) using TRIzol reagent (Life Technologies) and then purified using an RNeasy Mini kit (Qiagen). cDNA was synthesized using SuperScript III reverse transcriptase reagent (Life Technologies). .. The specific forward and reverse primers used for qRT-PCR were as follows: Nkx2-3 , 5′-tggccctgatgatgttacca-3′ and 5′-ggaaaactgcgtcccttcag-3′; Cdkn1a , 5′-tcccgactcttgacattgct-3′ and 5′-tccaaaatagaggggcagct-3′; Krt14 , 5′-gtacgagaagatggcggaga-3′ and 5′-ctttcatgctgagctgggac-3′; Vim , 5′-cagcagtatgaaagcgtgg-3′ and 5′-ggaagaaaaggttggcagag-3′; and glyceraldehyde 3-phosphate dehydrogenase ( Gapdh ), 5′-ggagcgagaccccactaacatc-3′ and 5′-ctcgtggttcacacccatcac-3′.

    Quantitative RT-PCR:

    Article Title: The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development
    Article Snippet: Paragraph title: RNA isolation and qRT-PCR analysis ... Total RNA was isolated from E14 mouse tissues (tooth, skin, lung, liver, kidney, heart, eye, and brain) and from molar tooth buds at each developmental stage (E11, E13, E14, E15, and E18) using TRIzol reagent (Life Technologies) and then purified using an RNeasy Mini kit (Qiagen). cDNA was synthesized using SuperScript III reverse transcriptase reagent (Life Technologies).

    Purification:

    Article Title: The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development
    Article Snippet: .. Total RNA was isolated from E14 mouse tissues (tooth, skin, lung, liver, kidney, heart, eye, and brain) and from molar tooth buds at each developmental stage (E11, E13, E14, E15, and E18) using TRIzol reagent (Life Technologies) and then purified using an RNeasy Mini kit (Qiagen). cDNA was synthesized using SuperScript III reverse transcriptase reagent (Life Technologies). .. The specific forward and reverse primers used for qRT-PCR were as follows: Nkx2-3 , 5′-tggccctgatgatgttacca-3′ and 5′-ggaaaactgcgtcccttcag-3′; Cdkn1a , 5′-tcccgactcttgacattgct-3′ and 5′-tccaaaatagaggggcagct-3′; Krt14 , 5′-gtacgagaagatggcggaga-3′ and 5′-ctttcatgctgagctgggac-3′; Vim , 5′-cagcagtatgaaagcgtgg-3′ and 5′-ggaagaaaaggttggcagag-3′; and glyceraldehyde 3-phosphate dehydrogenase ( Gapdh ), 5′-ggagcgagaccccactaacatc-3′ and 5′-ctcgtggttcacacccatcac-3′.

    Real-time Polymerase Chain Reaction:

    Article Title: The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development
    Article Snippet: Total RNA was isolated from E14 mouse tissues (tooth, skin, lung, liver, kidney, heart, eye, and brain) and from molar tooth buds at each developmental stage (E11, E13, E14, E15, and E18) using TRIzol reagent (Life Technologies) and then purified using an RNeasy Mini kit (Qiagen). cDNA was synthesized using SuperScript III reverse transcriptase reagent (Life Technologies). .. Expression of each gene was normalized to that of Gapdh . qRT-PCR was performed using iQ SYBR Green Supermix (Bio-Rad) with a CFX Connect Real-Time PCR detection system (Bio-Rad).

    Expressing:

    Article Title: The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development
    Article Snippet: Total RNA was isolated from E14 mouse tissues (tooth, skin, lung, liver, kidney, heart, eye, and brain) and from molar tooth buds at each developmental stage (E11, E13, E14, E15, and E18) using TRIzol reagent (Life Technologies) and then purified using an RNeasy Mini kit (Qiagen). cDNA was synthesized using SuperScript III reverse transcriptase reagent (Life Technologies). .. Expression of each gene was normalized to that of Gapdh . qRT-PCR was performed using iQ SYBR Green Supermix (Bio-Rad) with a CFX Connect Real-Time PCR detection system (Bio-Rad).

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    Thermo Fisher superscript iii reverse transcriptase reagent set
    Superscript Iii Reverse Transcriptase Reagent Set, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase reagent set/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase reagent set - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher mmlv reverse transcriptase superscript iii
    Mmlv Reverse Transcriptase Superscript Iii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmlv reverse transcriptase superscript iii/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mmlv reverse transcriptase superscript iii - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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