superscript iii reverse transcriptase kit  (Roche)

 
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    Structured Review

    Roche superscript iii reverse transcriptase kit
    Superscript Iii Reverse Transcriptase Kit, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase kit/product/Roche
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase kit - by Bioz Stars, 2020-04
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves
    Article Snippet: One μg of total RNA was reverse transcribed in 25 μl reaction mixture containing gene-specific complementary primer using Superscript III reverse transcriptase kit (Roche Diagnostics, Mannheim, Germany) by following the manufacturer's instructions. .. Amplified fragments specific to each gene were cloned separately into pCR 2.1-TOPO vector (Invitorgen, Carlsbad, CA) and recombinant clones purified using QIAGEN plasmid mini-prep kit (Qiagen Inc., Valencia, CA, USA).

    RNA Extraction:

    Article Title: Using iPSC‐derived human DA neurons from opioid‐dependent subjects to study dopamine dynamics, Using iPSC‐derived human DA neurons from opioid‐dependent subjects to study dopamine dynamics
    Article Snippet: Quantitative reverse transcription‐PCR (qRT‐PCR) Dopaminergic cultures were harvested at DIV28 for total RNA extraction following instructions from the manufacturer (RNAqueous, Ambion). .. Total RNA (1 μ g) was treated with RQ‐1 Rnase‐free Dnase I and reverse transcribed into cDNA using the Superscript III reverse transcriptase kit. cDNA levels for GAPDH, HPRT1, Nurr1, TH, DAT, VMAT2, Drd2, ptx3, mu, kappa, and delta opioid receptors were determined by specific universal probe library primer probe sets (Roche) using Roche Light Cycler II 480.

    Amplification:

    Article Title: Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves
    Article Snippet: A list of primer pairs and amplicon lengths are provided in Table . .. One μg of total RNA was reverse transcribed in 25 μl reaction mixture containing gene-specific complementary primer using Superscript III reverse transcriptase kit (Roche Diagnostics, Mannheim, Germany) by following the manufacturer's instructions.

    Isolation:

    Article Title: CART peptide induces neuroregeneration in stroke rats
    Article Snippet: Quantitative Reverse Transcription–Polymerase Chain Reaction The SVZ was dissected out from stroke brains and processed for RNA isolation and DNAse I treatment using the RNAqueous-Micro Ambion kit (Applied Biosystems, Grand Island, NY). .. Total RNA was reverse transcribed into cDNA using the Superscript III reverse transcriptase kit. cDNA levels for HPRT, Hmbs, BDNF, GDNF (glial cell line-derived neurotrophic factor), and bone morphogenetic protein-7 (BMP7) ( ) were determined by specific universal probe Library primer probe sets (Roche, South San Francisco, CA) using quantitative reverse transcription–polymerase chanin reaction (RT–PCR).

    Transfection:

    Article Title: Zoospore interspecific signaling promotes plant infection by Phytophthora
    Article Snippet: For each transfection, 24 μl of dsRNA (4 μg) was dried under vacuum (20-30 min) and then suspended in 10 μl PEG and 0.8 M mannitol solutions, respectively then incubated with 10 μl Lipofectin (Invitrogen) for 15 min prior to mixing with 20 μl P. capsici protoplasts. .. All the RNAs were treated with the RNase-Free DNAase Set (Qiagen), quantified and subjected to reverse transcription using the SuperScript III Reverse Transcriptase kit (Roche) followed by PCR using primers RPIPcapF: 5'- CAG ACG TCG CAG ATA CTA TTA ACC A-3'; and RPIPcapR: 5'-CTC CAG GAA GTA ATG CAT GAC ACA A-3' for RPI and actin housekeeping gene primers [ ] for an endogenous control.

    Quantitative RT-PCR:

    Article Title: Using iPSC‐derived human DA neurons from opioid‐dependent subjects to study dopamine dynamics, Using iPSC‐derived human DA neurons from opioid‐dependent subjects to study dopamine dynamics
    Article Snippet: Paragraph title: Quantitative reverse transcription‐PCR (qRT‐PCR) ... Total RNA (1 μ g) was treated with RQ‐1 Rnase‐free Dnase I and reverse transcribed into cDNA using the Superscript III reverse transcriptase kit. cDNA levels for GAPDH, HPRT1, Nurr1, TH, DAT, VMAT2, Drd2, ptx3, mu, kappa, and delta opioid receptors were determined by specific universal probe library primer probe sets (Roche) using Roche Light Cycler II 480.

    Purification:

    Article Title: Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves
    Article Snippet: One μg of total RNA was reverse transcribed in 25 μl reaction mixture containing gene-specific complementary primer using Superscript III reverse transcriptase kit (Roche Diagnostics, Mannheim, Germany) by following the manufacturer's instructions. .. Amplified fragments specific to each gene were cloned separately into pCR 2.1-TOPO vector (Invitorgen, Carlsbad, CA) and recombinant clones purified using QIAGEN plasmid mini-prep kit (Qiagen Inc., Valencia, CA, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves
    Article Snippet: Paragraph title: Primers, RT-PCR and analysis of gene sequences ... One μg of total RNA was reverse transcribed in 25 μl reaction mixture containing gene-specific complementary primer using Superscript III reverse transcriptase kit (Roche Diagnostics, Mannheim, Germany) by following the manufacturer's instructions.

    Article Title: Zoospore interspecific signaling promotes plant infection by Phytophthora
    Article Snippet: Paragraph title: Transient RPI gene silencing mediated by dsRNA and RT-PCR analysis ... All the RNAs were treated with the RNase-Free DNAase Set (Qiagen), quantified and subjected to reverse transcription using the SuperScript III Reverse Transcriptase kit (Roche) followed by PCR using primers RPIPcapF: 5'- CAG ACG TCG CAG ATA CTA TTA ACC A-3'; and RPIPcapR: 5'-CTC CAG GAA GTA ATG CAT GAC ACA A-3' for RPI and actin housekeeping gene primers [ ] for an endogenous control.

    Article Title: CART peptide induces neuroregeneration in stroke rats
    Article Snippet: .. Total RNA was reverse transcribed into cDNA using the Superscript III reverse transcriptase kit. cDNA levels for HPRT, Hmbs, BDNF, GDNF (glial cell line-derived neurotrophic factor), and bone morphogenetic protein-7 (BMP7) ( ) were determined by specific universal probe Library primer probe sets (Roche, South San Francisco, CA) using quantitative reverse transcription–polymerase chanin reaction (RT–PCR). ..

    Polymerase Chain Reaction:

    Article Title: Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves
    Article Snippet: One μg of total RNA was reverse transcribed in 25 μl reaction mixture containing gene-specific complementary primer using Superscript III reverse transcriptase kit (Roche Diagnostics, Mannheim, Germany) by following the manufacturer's instructions. .. Reverse transcription (RT) was carried out at 50°C for 30 min followed by thirty five consecutive cycles of PCR amplification (denaturation at 94°C for 30 s, annealing at 56°C for 30 s, extension at 72°C for 30 s), with a final extension at 72°C for 5 min using 1 μM each of gene-specific forward and reverse primers.

    Article Title: Zoospore interspecific signaling promotes plant infection by Phytophthora
    Article Snippet: .. All the RNAs were treated with the RNase-Free DNAase Set (Qiagen), quantified and subjected to reverse transcription using the SuperScript III Reverse Transcriptase kit (Roche) followed by PCR using primers RPIPcapF: 5'- CAG ACG TCG CAG ATA CTA TTA ACC A-3'; and RPIPcapR: 5'-CTC CAG GAA GTA ATG CAT GAC ACA A-3' for RPI and actin housekeeping gene primers [ ] for an endogenous control. ..

    Incubation:

    Article Title: Zoospore interspecific signaling promotes plant infection by Phytophthora
    Article Snippet: After further incubation for 24 h at 23°C, the mixture was transferred to 200 ml pea broth with ampicillin and vancomycin then 4 ml was transferred into each well of 12-well plates. .. All the RNAs were treated with the RNase-Free DNAase Set (Qiagen), quantified and subjected to reverse transcription using the SuperScript III Reverse Transcriptase kit (Roche) followed by PCR using primers RPIPcapF: 5'- CAG ACG TCG CAG ATA CTA TTA ACC A-3'; and RPIPcapR: 5'-CTC CAG GAA GTA ATG CAT GAC ACA A-3' for RPI and actin housekeeping gene primers [ ] for an endogenous control.

    DNA Sequencing:

    Article Title: Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves
    Article Snippet: One μg of total RNA was reverse transcribed in 25 μl reaction mixture containing gene-specific complementary primer using Superscript III reverse transcriptase kit (Roche Diagnostics, Mannheim, Germany) by following the manufacturer's instructions. .. Two independent clones were sequenced in both orientations by automated DNA sequencing at Molecular Biology Core facility at the Center for Reproductive Biology, Washington State University, Pullman, WA, USA.

    Expressing:

    Article Title: Using iPSC‐derived human DA neurons from opioid‐dependent subjects to study dopamine dynamics, Using iPSC‐derived human DA neurons from opioid‐dependent subjects to study dopamine dynamics
    Article Snippet: Total RNA (1 μ g) was treated with RQ‐1 Rnase‐free Dnase I and reverse transcribed into cDNA using the Superscript III reverse transcriptase kit. cDNA levels for GAPDH, HPRT1, Nurr1, TH, DAT, VMAT2, Drd2, ptx3, mu, kappa, and delta opioid receptors were determined by specific universal probe library primer probe sets (Roche) using Roche Light Cycler II 480. .. Relative expression level was calculated using ΔΔCt method compared to GAPDH as a reference gene.

    Article Title: Zoospore interspecific signaling promotes plant infection by Phytophthora
    Article Snippet: To determine RPI expression in dsRNA-treated lines, mycelia from each well (line) were subcultured and extracted for RNA on day 7 using the Qiagen RNeasy plant kit. .. All the RNAs were treated with the RNase-Free DNAase Set (Qiagen), quantified and subjected to reverse transcription using the SuperScript III Reverse Transcriptase kit (Roche) followed by PCR using primers RPIPcapF: 5'- CAG ACG TCG CAG ATA CTA TTA ACC A-3'; and RPIPcapR: 5'-CTC CAG GAA GTA ATG CAT GAC ACA A-3' for RPI and actin housekeeping gene primers [ ] for an endogenous control.

    Modification:

    Article Title: Zoospore interspecific signaling promotes plant infection by Phytophthora
    Article Snippet: Protoplasts were prepared using a modified transformation protocol for P. sojae [ ]. .. All the RNAs were treated with the RNase-Free DNAase Set (Qiagen), quantified and subjected to reverse transcription using the SuperScript III Reverse Transcriptase kit (Roche) followed by PCR using primers RPIPcapF: 5'- CAG ACG TCG CAG ATA CTA TTA ACC A-3'; and RPIPcapR: 5'-CTC CAG GAA GTA ATG CAT GAC ACA A-3' for RPI and actin housekeeping gene primers [ ] for an endogenous control.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Zoospore interspecific signaling promotes plant infection by Phytophthora
    Article Snippet: .. All the RNAs were treated with the RNase-Free DNAase Set (Qiagen), quantified and subjected to reverse transcription using the SuperScript III Reverse Transcriptase kit (Roche) followed by PCR using primers RPIPcapF: 5'- CAG ACG TCG CAG ATA CTA TTA ACC A-3'; and RPIPcapR: 5'-CTC CAG GAA GTA ATG CAT GAC ACA A-3' for RPI and actin housekeeping gene primers [ ] for an endogenous control. ..

    Recombinant:

    Article Title: Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves
    Article Snippet: One μg of total RNA was reverse transcribed in 25 μl reaction mixture containing gene-specific complementary primer using Superscript III reverse transcriptase kit (Roche Diagnostics, Mannheim, Germany) by following the manufacturer's instructions. .. Amplified fragments specific to each gene were cloned separately into pCR 2.1-TOPO vector (Invitorgen, Carlsbad, CA) and recombinant clones purified using QIAGEN plasmid mini-prep kit (Qiagen Inc., Valencia, CA, USA).

    Transformation Assay:

    Article Title: Zoospore interspecific signaling promotes plant infection by Phytophthora
    Article Snippet: Protoplasts were prepared using a modified transformation protocol for P. sojae [ ]. .. All the RNAs were treated with the RNase-Free DNAase Set (Qiagen), quantified and subjected to reverse transcription using the SuperScript III Reverse Transcriptase kit (Roche) followed by PCR using primers RPIPcapF: 5'- CAG ACG TCG CAG ATA CTA TTA ACC A-3'; and RPIPcapR: 5'-CTC CAG GAA GTA ATG CAT GAC ACA A-3' for RPI and actin housekeeping gene primers [ ] for an endogenous control.

    Plasmid Preparation:

    Article Title: Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves
    Article Snippet: One μg of total RNA was reverse transcribed in 25 μl reaction mixture containing gene-specific complementary primer using Superscript III reverse transcriptase kit (Roche Diagnostics, Mannheim, Germany) by following the manufacturer's instructions. .. Amplified fragments specific to each gene were cloned separately into pCR 2.1-TOPO vector (Invitorgen, Carlsbad, CA) and recombinant clones purified using QIAGEN plasmid mini-prep kit (Qiagen Inc., Valencia, CA, USA).

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  • 95
    Roche superscript iii cdna reverse transcriptase kit
    Superscript Iii Cdna Reverse Transcriptase Kit, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii cdna reverse transcriptase kit/product/Roche
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscript iii cdna reverse transcriptase kit - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    90
    Roche superscript iii reverse transcriptase kit
    Superscript Iii Reverse Transcriptase Kit, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase kit/product/Roche
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase kit - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

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