Structured Review

Promega superscript iii reverse transcriptase kit
Superscript Iii Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscript iii reverse transcriptase kit/product/Promega
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
superscript iii reverse transcriptase kit - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Real-time Polymerase Chain Reaction:

Article Title: High expression of estrogen-related receptor α is significantly associated with poor prognosis in patients with colorectal cancer
Article Snippet: The SuperScript III Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA) was used to synthesize obtained RNA into cDNA according to the manufacturer's instructions, with the temperature protocol as follows: 42°C for 30 min, 85°C for 5 sec, then holding at 4°C. .. The Step One Plus Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.) was applied for the qPCR assay using SYBR Green mix (Takara Bio, Inc., Otsu, Japan).

Amplification:

Article Title: High expression of estrogen-related receptor α is significantly associated with poor prognosis in patients with colorectal cancer
Article Snippet: The SuperScript III Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA) was used to synthesize obtained RNA into cDNA according to the manufacturer's instructions, with the temperature protocol as follows: 42°C for 30 min, 85°C for 5 sec, then holding at 4°C. .. The thermocycling conditions were as follows: Initial denaturation at 95°C for 5 min; followed by 40 amplification cycles of 95°C for 5 sec; annealing at 60°C for 15 sec; and elongation at 72°C for 15 sec. Ribosomal protein L13a (RPL13A) served as the internal control and the relative gene expression levels was determined by 2−ΔΔCq method ( ).

Size-exclusion Chromatography:

Article Title: High expression of estrogen-related receptor α is significantly associated with poor prognosis in patients with colorectal cancer
Article Snippet: .. The SuperScript III Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA) was used to synthesize obtained RNA into cDNA according to the manufacturer's instructions, with the temperature protocol as follows: 42°C for 30 min, 85°C for 5 sec, then holding at 4°C. .. The Step One Plus Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.) was applied for the qPCR assay using SYBR Green mix (Takara Bio, Inc., Otsu, Japan).

Quantitative RT-PCR:

Article Title: High expression of estrogen-related receptor α is significantly associated with poor prognosis in patients with colorectal cancer
Article Snippet: Paragraph title: RT-qPCR ... The SuperScript III Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA) was used to synthesize obtained RNA into cDNA according to the manufacturer's instructions, with the temperature protocol as follows: 42°C for 30 min, 85°C for 5 sec, then holding at 4°C.

SYBR Green Assay:

Article Title: High expression of estrogen-related receptor α is significantly associated with poor prognosis in patients with colorectal cancer
Article Snippet: The SuperScript III Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA) was used to synthesize obtained RNA into cDNA according to the manufacturer's instructions, with the temperature protocol as follows: 42°C for 30 min, 85°C for 5 sec, then holding at 4°C. .. The Step One Plus Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.) was applied for the qPCR assay using SYBR Green mix (Takara Bio, Inc., Otsu, Japan).

Expressing:

Article Title: High expression of estrogen-related receptor α is significantly associated with poor prognosis in patients with colorectal cancer
Article Snippet: The SuperScript III Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA) was used to synthesize obtained RNA into cDNA according to the manufacturer's instructions, with the temperature protocol as follows: 42°C for 30 min, 85°C for 5 sec, then holding at 4°C. .. The thermocycling conditions were as follows: Initial denaturation at 95°C for 5 min; followed by 40 amplification cycles of 95°C for 5 sec; annealing at 60°C for 15 sec; and elongation at 72°C for 15 sec. Ribosomal protein L13a (RPL13A) served as the internal control and the relative gene expression levels was determined by 2−ΔΔCq method ( ).

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    Promega superscript iii reverse transcriptase kit
    Superscript Iii Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase kit/product/Promega
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase kit - by Bioz Stars, 2020-04
    93/100 stars
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