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Fisher Scientific superscript iii reverse transcriptase kit
Superscript Iii Reverse Transcriptase Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscript iii reverse transcriptase kit/product/Fisher Scientific
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
superscript iii reverse transcriptase kit - by Bioz Stars, 2020-04
92/100 stars

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RNA Extraction:

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells
Article Snippet: RT-PCR analysis For all cell pelleting, cells were detached with 0.05% Trypsin–EDTA, washed twice with 1× PBS and stored at – 80 °C until RNA extraction. .. Equal concentrations of RNA (50 or 100 ng) was then reverse transcribed into cDNA using Superscript III Reverse Transcriptase Kit (Fisher Scientific, cat# 11752).

Amplification:

Article Title: Novel Reassortant Avian Influenza A(H5N1) Virus in Human, Southern Vietnam, 2014
Article Snippet: .. The 8 viral genes were amplified with SuperScript III Reverse Transcriptase Kit (Fisher Scientific, Pittsburg, PA, USA) and Phusion High-Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) with specific paired primers, according to the manufacturer’s instructions, and sequenced on an ABI 3730 automated sequencer with Big-Dye Terminator Cycle Sequencing reagents (Applied Biosystems, Foster City, CA, USA). ..

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells
Article Snippet: Equal concentrations of RNA (50 or 100 ng) was then reverse transcribed into cDNA using Superscript III Reverse Transcriptase Kit (Fisher Scientific, cat# 11752). .. PCR amplification was conducted using HotStarTaq Polymerase (Qiagen, cat# 203645).

Isolation:

Article Title: Novel Reassortant Avian Influenza A(H5N1) Virus in Human, Southern Vietnam, 2014
Article Snippet: Virus was isolated by inoculating the throat swab specimens into 10-day-old embryonated chicken eggs; the resulting isolate, A/Vietnam/14011801/2014 (GISAID accession nos. .. The 8 viral genes were amplified with SuperScript III Reverse Transcriptase Kit (Fisher Scientific, Pittsburg, PA, USA) and Phusion High-Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) with specific paired primers, according to the manufacturer’s instructions, and sequenced on an ABI 3730 automated sequencer with Big-Dye Terminator Cycle Sequencing reagents (Applied Biosystems, Foster City, CA, USA).

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells
Article Snippet: Total RNA was isolated from all treatment groups using RNeasy Kit (Qiagen, cat# 74134). .. Equal concentrations of RNA (50 or 100 ng) was then reverse transcribed into cDNA using Superscript III Reverse Transcriptase Kit (Fisher Scientific, cat# 11752).

Infection:

Article Title: Novel Reassortant Avian Influenza A(H5N1) Virus in Human, Southern Vietnam, 2014
Article Snippet: H5N1 virus infection was detected in the patient’s throat swab specimen by real-time reverse transcription PCR at the Pasteur Institute in Ho Chi Minh City. .. The 8 viral genes were amplified with SuperScript III Reverse Transcriptase Kit (Fisher Scientific, Pittsburg, PA, USA) and Phusion High-Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) with specific paired primers, according to the manufacturer’s instructions, and sequenced on an ABI 3730 automated sequencer with Big-Dye Terminator Cycle Sequencing reagents (Applied Biosystems, Foster City, CA, USA).

Sequencing:

Article Title: Novel Reassortant Avian Influenza A(H5N1) Virus in Human, Southern Vietnam, 2014
Article Snippet: .. The 8 viral genes were amplified with SuperScript III Reverse Transcriptase Kit (Fisher Scientific, Pittsburg, PA, USA) and Phusion High-Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) with specific paired primers, according to the manufacturer’s instructions, and sequenced on an ABI 3730 automated sequencer with Big-Dye Terminator Cycle Sequencing reagents (Applied Biosystems, Foster City, CA, USA). ..

Polymerase Chain Reaction:

Article Title: Novel Reassortant Avian Influenza A(H5N1) Virus in Human, Southern Vietnam, 2014
Article Snippet: H5N1 virus infection was detected in the patient’s throat swab specimen by real-time reverse transcription PCR at the Pasteur Institute in Ho Chi Minh City. .. The 8 viral genes were amplified with SuperScript III Reverse Transcriptase Kit (Fisher Scientific, Pittsburg, PA, USA) and Phusion High-Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) with specific paired primers, according to the manufacturer’s instructions, and sequenced on an ABI 3730 automated sequencer with Big-Dye Terminator Cycle Sequencing reagents (Applied Biosystems, Foster City, CA, USA).

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells
Article Snippet: Equal concentrations of RNA (50 or 100 ng) was then reverse transcribed into cDNA using Superscript III Reverse Transcriptase Kit (Fisher Scientific, cat# 11752). .. PCR amplification was conducted using HotStarTaq Polymerase (Qiagen, cat# 203645).

Activity Assay:

Article Title: Novel Reassortant Avian Influenza A(H5N1) Virus in Human, Southern Vietnam, 2014
Article Snippet: The 8 viral genes were amplified with SuperScript III Reverse Transcriptase Kit (Fisher Scientific, Pittsburg, PA, USA) and Phusion High-Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) with specific paired primers, according to the manufacturer’s instructions, and sequenced on an ABI 3730 automated sequencer with Big-Dye Terminator Cycle Sequencing reagents (Applied Biosystems, Foster City, CA, USA). .. The neuraminidase gene possessed no amino acid substitutions associated with decreased antiviral activity, nor did the virus have amino acid substitutions associated with increased adaptation, virulence, infectivity, or transmissibility in mammalian hosts, including the E627K and D701N mutations in polymerase basic protein 2 ( ).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells
Article Snippet: Paragraph title: RT-PCR analysis ... Equal concentrations of RNA (50 or 100 ng) was then reverse transcribed into cDNA using Superscript III Reverse Transcriptase Kit (Fisher Scientific, cat# 11752).

Staining:

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells
Article Snippet: Equal concentrations of RNA (50 or 100 ng) was then reverse transcribed into cDNA using Superscript III Reverse Transcriptase Kit (Fisher Scientific, cat# 11752). .. PCR product gel images were obtained using SYBR safe DNA gel stain (Fisher Scientific, cat# 1760400) and Gel Doc XR+ system (BioRad).

Binding Assay:

Article Title: Novel Reassortant Avian Influenza A(H5N1) Virus in Human, Southern Vietnam, 2014
Article Snippet: The 8 viral genes were amplified with SuperScript III Reverse Transcriptase Kit (Fisher Scientific, Pittsburg, PA, USA) and Phusion High-Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) with specific paired primers, according to the manufacturer’s instructions, and sequenced on an ABI 3730 automated sequencer with Big-Dye Terminator Cycle Sequencing reagents (Applied Biosystems, Foster City, CA, USA). .. By gene sequencing analysis, A/Vietnam/14011801/2014 was found to have the multibasic cleavage site of hemagglutinin (HA) protein, which indicates highly pathogenic avian influenza A(H5N1) viruses, and was shown to predict binding specificity to an avian α2,3 sialic acid receptor.

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    Fisher Scientific superscript iii reverse transcriptase kit
    Superscript Iii Reverse Transcriptase Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase kit/product/Fisher Scientific
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase kit - by Bioz Stars, 2020-04
    92/100 stars
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